This part specifies the methods and requirements for field efficacy plot tests of fungicides against strawberry powdery mildew (Sphaerotheca macularis). This part applies to field efficacy plot tests and efficacy evaluation of fungicides for registration of strawberry powdery mildew control. Other field efficacy tests shall refer to this part. GB/T 17980.119-2004 Guidelines for field efficacy tests of pesticides (II) Part 119: Efficacy test of fungicides against strawberry powdery mildew GB/T17980.119-2004 Standard download decompression password: www.bzxz.net
This part specifies the methods and requirements for field efficacy plot tests of fungicides against strawberry powdery mildew (Sphaerotheca macularis). This part applies to field efficacy plot tests and efficacy evaluation of fungicides for registration of strawberry powdery mildew control. Other field efficacy tests shall refer to this part.

GB/T17980.119—2004
Field efficacy test is an important part of pesticide registration management and an important technical basis for the formulation of pesticide product labels. Labels are the only guide for the safe and rational use of pesticides. In order to standardize the methods and contents of pesticide field tests, make the tests more scientific and unified, and align with international standards, so that my country's efficacy test reports have international recognition, the national standards for my country's field efficacy test guidelines are specially formulated. This series of standards refers to the field efficacy test guidelines of the European and Mediterranean Plant Protection Organization (EPPO) and similar guidelines of the Food and Agriculture Organization of the United Nations (FAO) in the Asia-Pacific region. It is formulated based on my country's actual situation and verified by a large number of field efficacy tests. Strawberry powdery mildew is one of the important diseases of grass crops in my country, and fungicides are often used for prevention and control in production. In order to determine the optimal dosage of pesticides for the prevention and control of strawberry powdery mildew, test the effects of pesticides on crops and non-target beneficial organisms, and provide a basis for the efficacy evaluation and safe and rational use technology of fungicide registration, this part of GB/T17980 is specially formulated. This part is one of the standards of the Guidelines for Field Efficacy Tests of Pesticides (II), but it is an independent part. This part was proposed by the Ministry of Agriculture of the People's Republic of China. The drafting unit of this part: Institute for the Control of Pesticides of the Ministry of Agriculture. The main drafters of this part: Wu Xinping, Li Gou, Liu Naizhi, Gu Baogen, Chen Liping, Xiao Bin, Zhang Xiaofeng. This part is interpreted by the Institute for the Control of Pesticides of the Ministry of Agriculture. Scope
Guidelines for Field Efficacy Tests (II)
Part 119: Fungicides for Control of Strawberry Powdery Mildew GB/T 17980.119-2004
This part specifies the methods and requirements for field efficacy plot tests of fungicides for control of grass powdery mildew (Sphaerotheca macularis). This part is applicable to field efficacy plot tests and efficacy evaluation for registration of fungicides for control of strawberry powdery mildew. Other field efficacy tests shall be carried out in accordance with this part.
2 Experimental conditions
2.1 Selection of crop varieties and test subjects
The test subject is powdery mildew.
The test crop is strawberry. Select susceptible varieties and record the variety name. 2.2 Environmental conditions
Field trials should be conducted in areas where strawberry is grown on a large scale and where powdery mildew has occurred seriously in previous years. The cultivation conditions (such as soil type, fertilization, variety, planting density, etc.) of all test plots should be consistent and in line with local scientific agricultural practices (GAP). If fumigants and smoke sprays are tested in greenhouses, each treatment should use a single greenhouse or isolation room. 3 Experimental design and arrangement
3.1 Agents
3.1.1 Test agents
Indicate the agent's trade name or code, generic name, Chinese name, dosage form content and manufacturer. The test agent treatment should be no less than three doses or the dosage specified in the agreement (the test agreement signed by the test entrusting party and the test undertaking party). 3.1.2 Control Agents
Control agents should be registered products that have been proven to have good efficacy in practice. The type and mode of action of control agents should be similar to those of test agents and the local commonly used dosage should be used. Special circumstances may depend on the purpose of the test. 3.2 Plot Arrangement
3.2. 1 Plot Arrangement
The plots of test agents, control agents and blank controls are arranged randomly. Special circumstances should be explained. 3.2.2 Plot Area and Repetition
Plot area: 15m2~50m2, with a greenhouse of not less than 8m2. Number of Repetitions: not less than 4 repetitions.
3.3 Application Method
3.3.1 Usage Method
Carry out according to the requirements of the agreement and the instructions on the label. The application of pesticides should be compatible with local scientific agricultural practices. 3.3.2 Types of equipment used
Use commonly used equipment in production and record all information on the type of equipment used and operating conditions (such as working pressure, nozzle diameter). The amount of medicine applied should be accurate and evenly distributed. Any deviation in the amount of medicine applied should be recorded if it exceeds ±10%. 1
GB/T 17980.119—2004
3.3.3 Time and number of times of application
Follow the requirements of the agreement and the instructions on the label. Usually, the first application of medicine is carried out at the early stage of the disease or when dark spots appear on the back of the leaves of strawberry plants and high temperature and high humidity appear alternately. The second application of medicine can be determined according to the development of the disease and the duration of the medicine. 3.3.4 Dosage and capacity
Use according to the requirements of the agreement and the dosage indicated on the label. Usually, the content of active ingredients is expressed as g/hm2. When used for spraying, record the multiple of medicine used and the amount of liquid medicine used per public item (L/hm). 3.3.5 Requirements for the data of pesticides used to control other pests and diseases If other pesticides are to be used, pesticides that have no effect on the test pesticides and test objects should be selected, and all test plots should be treated uniformly. They should be used separately from the test pesticides and control pesticides to minimize the interference of these pesticides and record the accurate data of the application of such pesticides.
4 Investigation, recording and measurement methods
4.1 Meteorological and soil data
4.1.1 Meteorological data
During the test, rainfall (rainfall type and daily rainfall, expressed in mm) and temperature (daily average temperature, maximum and minimum temperature, expressed in °C) data should be obtained from the test site or the nearest meteorological station. Severe climatic factors that affect the test results during the entire test period, such as severe or long-term drought, heavy rain, ice signs, etc., should be recorded. 4.1.2 Soil data
Record soil type, soil fertility, moisture (dry, wet or waterlogged), soil cover (such as crop residues, plastic film covering, weeds) and other data.
4.2 Survey method, time and frequency
4.2.1 Survey method
Sampling at five points on the diagonal of each plot, and surveying 3 plants at each point. Survey all leaves of each plant. Grading method:
Grade 0: No disease spots,
Grade 1: The area of ​​disease spots accounts for less than 5% of the total leaf area; Grade 3: The area of ​​disease spots accounts for 6% to 15% of the total leaf area, Grade 5: The area of ​​disease spots accounts for 16% to 25% of the total leaf area; Grade 7: The area of ​​disease spots accounts for 26% to 50% of the total leaf area; Grade 9: The area of ​​disease spots accounts for more than 51% of the total leaf area. 4.2. 2 Survey time and frequency
Carry out according to the requirements of the agreement. Usually, the disease base is investigated before applying pesticides, and the control effect is investigated before the next application and 7 to 10 days after the last application.
4.2.3 Calculation method of drug efficacy
Disease index = Z (number of diseased leaves at each level × relative level value) × 100...(1)
Total number of leaves investigated × 9
Pre-drug disease index of blank control area × pre-drug disease index of treated area/or control effect (no base before application) (%) Blank control area disease index × 100 (2) Control effect (%) = (1-
Pre-drug disease index of blank control area × pre-drug disease index of treated area/or control effect (no base before application) (%) ）Disease index of blank control area
4.3 Other effects on crops
Observe whether crops are injured by pesticides. If so, record the symptoms and extent of the injury. In addition, the beneficial effects on fruit trees (such as promoting maturity, stimulating growth, etc.) should also be recorded. Use the following methods to record pesticide damage:
If the pesticide damage can be measured or calculated, it should be expressed in absolute values. b) In other cases, the extent and frequency of pesticide damage can be estimated by the following two methods: GB/T17980.119—2004
1) Record the pesticide damage situation of each plot according to the pesticide damage grading method, expressed as one, ten, ten ten, ten ten ten, and ten ten ten ten. Grading method of pesticide damage: bZxz.net
One: No pesticide damage;
Ten: Mild pesticide damage, no impact on normal crop growth; Ten Ten: Obvious pesticide damage, recoverable, no crop yield reduction; Ten Ten Ten: Severe pesticide damage, affecting normal crop growth, causing certain losses to crop yield and quality, generally requiring compensation for part of the economic losses;
Ten Ten Ten Ten: Severe pesticide damage, crop growth is hindered, yield and quality losses are serious, and economic losses must be compensated. 2) Evaluate the percentage of pesticide damage in each test plot compared with the blank control. At the same time, the crop pesticide damage symptoms (dwarfing, chlorosis, deformity) should be accurately described, and physical photos, videos, etc. should be provided. 4.4 Impact on other organisms
4.4.1 Impact on other pests and diseases
Any signs of impact on other pests and diseases should be recorded. 4.4.2 Impact on other non-target organisms
Record the impact of the pesticide on wild organisms and beneficial insects in the test area. 4.5 Product yield and quality
The yield of each plot should be recorded, expressed in kg/hm2. 5 Results
The results obtained from the experiment should be analyzed by biostatistical methods (using DMRT method), and a conclusion report should be written in a formal format, and the test results should be analyzed. The original data should be kept for inspection and verification.
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