This standard specifies the detection method and reagents for lymphocytic choriomeningitis virus (LCMV). This standard is applicable to the detection of LCMV in mice, guinea pigs and hamsters. GB/T 14926.18-2001 Detection method for lymphocytic choriomeningitis virus in experimental animals GB/T14926.18-2001 Standard download decompression password: www.bzxz.net

ICS65.020.30
National Standard of the People's Republic of China
GB/T14926.18—2001
Laboratory animal
Microbiological examination methods (4)
Laboratory animalMicrobiological examination methods2001-08-29Promulgated
People's Republic of China
General Administration of Quality Supervision, Inspection and Quarantine
Implementation on 2002-05-01
GB/T14926.18—2001
This standard is a revision of GB/T14926.18—1994 (Detection method for lymphocytic choriomeningitis virus in laboratory animals). This standard excludes the provisions of GB/T14926.18—1994 Enzyme-linked immunosorbent assay, indirect immunofluorescence assay and immunoenzyme staining method
This standard is proposed and approved by the Ministry of Science and Technology of the People's Republic of China. The drafting unit of this standard is the Chinese Laboratory Animal Society. The main drafter of this standard is He Zhengming.
This standard was first issued in January 1994.
National Standard of the People's Republic of ChinabzxZ.net
Laboratory animal-Method for examination of lymphocytic choriomeningitis virus (LCMW) This standard specifies the following standards. This standard is applicable to small surface products. 2 Reference standards The following standards are located in the relevant documents and are used in the relevant documents. All standards have been revised and GB/T1492O 2001 Experimental Procedure GB/T149 GB/T149 3 Principle 2001 Experimental Enzyme Immunoassay 2001 Experimental Enzyme Immunoassay for LCMV Main Reagents and Apparatus 4.1 Test Method: ||4.1.1 ELISA Antibodies
4.1.1.1 Specific Antibodies
GB/T14926-18-2001
Represents GB/T149261F-1994
The versions shown are the latest versions of the standards when the standards are published.
LCMV
In a biological safety cabinet, use Vero
+, harvest the culture, thaw three times, and then concentrate the cell supernatant by ultra-vibration to form ELISA antigens. Or after ultrasonic treatment, centrifuge at low speed to remove antigens. 4.1.1.2 Normal Antigens
After Vero cells are frozen and thawed, the supernatant is centrifuged at low speed and high speed to obtain the ELISA antigens. 4.1.2 Antigen slices
In a biosafety cabinet, LCMV infected Vero cells, replaced Vero cells every 2-3 days, cultured for 7-10 days, and measured intracellular specific fluorescence by IFA method. When the fluorescence reached +10+~+10+, the cells were digested and dispersed with trypsin, washed with PBS, smeared, dried at room temperature, irradiated at 20cm below the line for 30 minutes, fixed with cold ketone for 10 minutes, and stored at -20°C. 4.1.3 Positive serum
Use β-propiolactone to infect LCMV antigen, and immunoprecipitate or antiserum obtained from SPF mice, guinea pigs, and hamsters. 4.1.4 Anti-atrial serum
Approved by the General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China on August 29, 2001 and implemented on May 1, 2002
Antiserum obtained from SPF mice, guinea pigs, and hamsters. 4.1.5 Static conjugates
GB/T14926-18-2001
Horseradish peroxidase-labeled goat or rabbit anti-mouse, guinea pig, hamster IgG antibodies, used to detect serum antibodies of related animals: horseradish peroxidase-labeled serotype ASPA) can be used to detect serum antibodies of related animals, guinea pigs, and hamsters. 4.1-6 Fluorescein isocyanate-labeled goat or rabbit anti-mouse, guinea pig, and hamster IgG antibodies, used to detect serum antibodies of related animals. 4.2.1. Labels only.
4.2.2 Fluorescence microscope.
4.2.3 Ordinary microscope.
42.437℃ incubator or water box.
5 Detection method
51 ELISA method
52 IFA method
5.3 IEA method
Sperm determination
B/T149
Sperm selection network A large
For positive test
Sperm report
Make a report based on the judgment.
If positive, light is used
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