Pesticide-Guidelines for the field efficacy trials(II)-Part 145:Plant growth regulator efficacy trials on bloom and furit set of fruiter
other information
Release date:2004-03-03
Review date:2004-10-14
drafter:Liu Xue, Jia Fuqin, Zhou Yushu, Zhang Jia, Ye Guibiao, Jiang Guokeng, Li Aihua
Drafting unit:Pesticide Testing Institute, Ministry of Agriculture
Focal point unit:Ministry of Agriculture of the People's Republic of China
Proposing unit:Ministry of Agriculture of the People's Republic of China
Publishing department:General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China Standardization Administration of China
competent authority:Ministry of Agriculture
Some standard content:
National Standard of the People's Republic of China
GB/T 17980.145—2004
Guidelines for the field efficacy trials (II)
Part 145: Plant growth regulator trials on flowering and fruit setting of fruit trees
Pesticide-
Guidelires for the field efficacy trials (I)-Part 145; Plant growth regulator trials on flowering and fruit setting of fruit trees fruiler2004-03-03 Issued
General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China Standardization Administration
2004-08-01 Implementation
GB/T17980.145--2004
The four-day efficacy test is one of the most important contents of the pesticide registration work in China, and is an important technical basis for the formulation of pesticide product labels, and the label is the only guide for the safe and reasonable use of pesticides. In order to standardize the content of the pesticide efficacy test method, make the test files scientific and unified, and make the efficacy test reports with international standards and international recognition, my country has formulated the national standards for the efficacy test criteria. This series of standards refers to the day-to-day efficacy test criteria of the European and Mediterranean Plant Protection Organization (EHP) and the standards of the Asia-Pacific region of the Joint Secretariat for the Prevention of Pesticides, and is formulated based on the actual situation in my country and verified by the French or international standards. In the process of fruit production, it can regulate the growth of plants. It can promote early flowering and fruit setting of trees and improve the quality of fruit yield. In order to determine the best time to use the control agent, dosage, biological effects on trees and non-fertilizers, and to reduce the growth of fruit trees, the efficacy evaluation of fruit regulators should be carried out according to the reasonable use of the technology, and the Ministry of Agriculture of the People's Republic of China has formulated GB/117980, which is part of the series of standards for the efficacy test of the standard (I) - but this point is an independent part. The main body of this part is proposed by the Ministry of Agriculture of the People's Republic of China. The main person in charge of this part is Liu Xue, Yan Fukan, Zhang Banye, Jiang Guolin, Li Ehua. This part is responsible for the interpretation of the Ministry of Agriculture. 5
1 Standard for the efficacy test of the standard (II)
GB/T 17980.145-2004
Part 145: Plant growth regulators to promote flowering and fruit setting in fruit trees This part specifies the method and basic requirements for the efficacy plot test of plant growth regulators on flowering and fruit setting in fruit trees. This part is applicable to the registration of plant growth regulators for flowering and fruit setting in fruit trees. Other daytime efficacy tests shall be carried out in accordance with this part.
2 Test conditions
2.] Selection of crops and grain varieties||t t||The fruiting varieties and trees used in the experiment should be representative of the local orchards, with proven varieties and names of the species. 2.2 Cultivation conditions
The experiment was conducted in a representative orchard, with apple trees aged 10-11 years, vigorous and with fewer fruits being the most suitable. The cultivation conditions (such as type, cropping) of all test trees should be uniform and consistent with the local scientific agricultural practices.
3 Experimental design and arrangement
3.1 Pesticides
3. 1.1 The test agent
should indicate the drug product name, public name, Chinese name or code, dosage form, chemical composition, and manufacturer. The test agent has four variables: full treatment, low-medium and medium doses (the multiple of the medium dose is set to score the safety of the test agent to crops) or the commercial dose set according to the agreement (signed by the test entrusting party and the test organizer). 3.1.2 For instantaneous agents
For intermediate agents, they should be registered: products that have been proven to have good efficacy in actual practice. Once the situation permits, the type and mode of action of the agent should be similar to the local dose of the test agent. Special circumstances may depend on the purpose of the test. The test agent should be used according to the purpose of the test. For the mud agent, each single plant in the mixture should be set up as a control. 3.2 Plot Arrangement
3.2.1 Plot Arrangement
The experimental agent, control agent and blank control plots should be arranged randomly. 3.2.2 Plot Accumulation and Insufficiency
Small K out! 2 plants--3 flavors.
Review, at least 1 control.
3.3 Application Method
3.3.1 Application Method
Follow the method indicated on the label or according to the efficacy requirements. Generally, spraying and other methods are used for application. 3.3.2 Type of Equipment Used
The equipment should be used in accordance with the plan: and the results should ensure that the application is accurate and evenly distributed. If the amount of medicine used exceeds the limit, it must be recorded. All data on the type and working conditions (such as working pressure, nozzle diameter) of the equipment used in the industry, 51:
GB/T179B0.145-2004
3.3.3 Application time and frequency
The time and frequency of application shall be in accordance with the instructions of the standard or the requirements of the agreement: record the number of applications, the dosage and date of the application. 3.3.4 Application electricity and water
The dosage shall be applied according to the dosage recommended in the agreement. The dosage of the drug shall be expressed in terms of active ingredient number/hm or multiples: if it is expressed in multiples, the amount of liquid must be stated: the water use station shall be expressed in L/hr. 3.3.5 Requirements for pesticide application information When pesticides are used to prevent weeds and insect pests, they should be applied uniformly in all plots. Test and control pesticides should be used separately. The amount of pesticides should be controlled within a certain range. The accurate information of such pesticide application (such as pesticide name, application time, pesticide application schedule, and measurement methods) should be recorded. 4.1 Meteorological and soil data 4.1. 1 Weather data
The data for the entire test period should be obtained from the nearest weather station in Shantou. If necessary, please provide the type and amount of precipitation, daily average temperature (expressed in Sichuan), wind speed, relative brightness and other information. The attached is the meteorological data before and after the application of pesticides.
All adverse climatic factors that affect the test performance during the entire test period, such as long-term drought, severe cold, ice and other conditions, must be recorded: 4.1.2 Soil data
Record the type of terrain, soil erosion conditions and soil fill materials such as weeds and intercrops. 4.2 Field management
Prepare current information such as water supply and drainage,
4.3 Investigation methods, time and frequency
4.3.1 Investigation methods and types
Select one fruit tree for each test, check the total number of flowering steps and the number of short fruit branches of the whole tree! Total length inspection number, crown height, crown straightness and lightness: mark the length according to the four directions of east, south, west and north, or mark the lower branches of the air bones according to the two directions of east and west, investigate the number of inflorescences, the dullness rate, the three average single fruit weights, the new growth and decline (including nested and ball oranges) and the length of the nodes: measure the yield of a single plant. 4.3.2 Investigation time and frequency
In the year of application, after the growth of autumn shoots stops, check the length of new shoots, the length of the tree crown, and the inner diameter of the crown; during the harvest period, check the average single fruit weight and yield per plant.
In some years of application, during the inflorescence height period, investigate the total flowering period of the whole tree, the number of short fruit branches, the number of medium and long fruit branches, the number of central flowering branches after the fruit drop, and the number of flowering branches in the middle flowering period. After the spring flowering period, investigate the length of new shoots, the length of node, the height of the crown, and the diameter of the crown. In the harvest period, investigate the average single fruit weight and yield per plant. 4.3.3 Efficacy estimation method
According to the relevant provisions of the following instruments: the total number of buds in the whole tree, the effect of the sequence, the fruit setting rate, the short fruit rate, the length of new shoots, the length of node, the height of the crown, Crown diameter, average and single fruit weight.
4.4 Other effects on crops
Record the type and degree of damage to crops caused by pesticide removal. In addition, other effects on the trees such as improved fruit yield, improved fruit shape index, etc. are also recorded. The following methods are used to record the pesticide card:
a) Fruit shadow protection is marked or cut, and it must be expressed in absolute numbers, such as fruit setting rate, single fruit number, single plant yield, etc. ) The frequency and degree of damage can be expressed by two evaluation methods: 1) Use the pesticide damage grading standard to distinguish the pesticide damage level in each plot: one, ten, eleven, ten plus ten, and eleven plus one. No pesticide:
Ten mild damage, does not affect the normal growth of crops: Ten obvious pesticide damage, can be repaired, will not cause full yield of crops, GB/1 17980.145—2004
11.1 Drug damage, crop growth stunting, yield or quality loss of black trees to a certain extent; 2.1.2 Production damage, crop growth stunting, yield or quality loss, 2.1.3 Annual test area, self-test ...1 Effect on pests and diseases
Record any effects of the agent on pests and diseases, including beneficial and unbeneficial effects: 4.5.2 Effect on other organismswwW.bzxz.Net
Record any effects of the agent on bison and (or) beneficial organisms. 6 Effect on fruit quality
At harvest, randomly select 10 fruits from four locations in the east and north and three locations in the top and bottom of each plot to measure the changes in the fruits, including the ends, ends, etc. 5 Results
Use biostatistics to calculate and analyze the test results, specify the statistical method used, write the test report in a formal format, and explain the test results. The original data should be kept for verification. 4
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