NY/T 2427-2013 Guidelines for testing specificity, consistency and stability of new plant varieties - Bean
Some standard content:
ICS 65.020.20
Agricultural Industry Standard of the People's Republic of China
NY/T2427—2013
Guidelines for the conduct of tests for distinctness, uniformity and stability-French bean
(Phaseolus vulgaris L.)
(UPOV:TG/12/9,Guidelines for the conduct of tests for distinctness, uniformity and stabilityFrench bean
(Phaseolus vulgaris L.)
(UPOV:TG/12/9, bean,NEQ)2013-09-10Promulgated
2014-01-01Implementation
Ministry of Agriculture of the People's Republic of China
Scope,
Normative references
Terms and definitions
Requirements for propagation materials
Test methods
Determination of specificity, consistency and stability resultsCharacter table
Grouped traits
10 Technical questionnaire
Appendix A (Normative Appendix)
Clearance B Normative Appendix)
Appendix ((Normative Appendix)
Character table of kidney beans
Interpretation of the character table of kidney beans
Format of technical questionnaire on kidney beans
NY/T 24272013
NY/T24272013
This standard was drafted in accordance with the rules given in GB/T1.1-2009. This standard was revised using the redrafting method and adopted the Guidelines for the conduct of tests for distinctness, uniformity and stability - French bean (Phoseulus vutgurisL) of the International Union for the Protection of New Varieties of Plants (UPOV) TG/12/9.
This standard corresponds to the UPOV Guidelines TG/12/9 and is not equivalent to IG/12/9. There are technical differences between this standard and the UPOV Guidelines TG/12/9. The main differences are as follows: 1. Added "white seed coat varieties: immature grain color (early stage of fluorescent swelling)", "beauty: texture type", "sunflower: stripe color intensity", "seed coat: stripe shape", "seed: navel ring color" 5 Individual traits; · Deleted three botanical traits, namely "thickness", "seed length" and "seed coat: secondary color spot density" and four traits, namely "resistance to bean charcoal blight", "resistance to bean common mosaic virus", "resistance to bean bacterial leaf spot" and "resistance to bean bacterial blight"; · Adjusted the expression status of five traits, namely "leaf, degree of green", "leaf, degree of concavity and convexity of leaf surface", "shape of terminal brook", "stripe color" and "curvature of beak".
This standard was proposed by the Science, Technology and Education Department of the Ministry of Agriculture and is under the jurisdiction of the National Technical Committee for Testing and Standardization of New Plant Varieties (SAC/TC277). Drafting units of this standard: Vegetable and Flower Research Institute of Chinese Academy of Agricultural Sciences, Vegetable and Flower Research Institute of Gulin Province, Science and Technology Development Center of the Ministry of Agriculture. Drafters of this standard: Xu Zhaoniu, Zhang Helong, Xu Liming, Xie Neiyan, Gao Jiande, Jian Hong, Liu Su, Du Yuanyuan. 1 Scope
NY/T 2427—2013
Guidelines for specificity, consistency and stability testing of new plant varieties
This standard specifies the technical requirements and general principles for result determination for specificity, consistency and stability testing of new varieties of kidney beans. This standard applies to specificity, consistency and stability testing and result determination of new varieties of Phaseulus vulgaris L. 2 Normative references
The following documents are indispensable for the application of this document. For dated references, only the dated versions apply to this document. For all undated references, the latest versions (including all amendments) apply to this document. GB/T 19557.1 Guidelines for testing specificity, consistency and stability of new plant varieties General Principles 3 Terms and definitions:
The terms and definitions defined in GB/T 19557.1 and the following terms and definitions apply to this document. 3.1
Population measurement single measurement of a group of plants or parts of plants A group record is obtained by measuring the organs or parts of a group of model plants. 3.2
Individual measurement of a nuinber of individual plants or parts of plants A group record is obtained by measuring the organs or parts of a group of plants one by one. 3.3
Visual assessment of a group: visual assessment by a single observation of a group of plants or parts of plants A group record is obtained by visually observing an organ or part of a group of plants or plants. 3.4
Visual assessment by observation of individual pltants or parts of plainis A set of individual records is obtained by visually observing a group of plants or a particular organ or part of a plant. Symbols
The following symbols apply to this document:
MG: Group measurement.
MS: Individual measurement.
VG: Group visual assessment.
VS: Individual visual assessment.
QL: Qualitative trait,
QN: Quantitative trait.
PQ: Pseudo-quality trait.
*: The indicated traits are those that are considered important by UPOV for the purpose of uniform variety description. All UPOV members shall use these traits, except for the expression state test for traits that are limited by environmental conditions. (a): The notation is explained in detail in B.2. NY/T2427—2013
(）: The marked content is explained in detail in B.3. 1: The underlined content in this document is a special reminder of the scope of application of the test trait, C: creeping type,
D: dwarf type.
5 Requirements for propagation materials
5.1 Propagation materials are provided in the form of seeds.
5.2 The number of seeds submitted shall be at least 20,000 seeds. 5.3 The submitted propagation materials shall be healthy in appearance, high in vitality, and free from pests and diseases. The specific quality requirements for propagation materials are as follows: purity 299.0%, germination rate 290%, moisture ≤12%. 5.4 The submitted propagation materials are generally not subjected to any treatment that affects the normal expression of variety traits (such as seed coating treatment). If they have been treated, detailed descriptions of the treatment should be provided.
5.5 The submitted propagation materials shall comply with the relevant provisions of China's plant quarantine. 6 Test methods
6.1 Test cycle
The test cycle is at least 2 independent growth cycles. 6.2 Test location
The test is usually carried out at one location. If some traits cannot be fully expressed at this location, they can be observed at other qualified locations.
6.3 Field test
6.3.1 Test design
The medium variety and similar varieties are planted adjacent to each other. Sow 2 to 3 seeds in a hole, and leave 1 seedling in each hole. Use appropriate plant spacing. At least 150 plants of dwarf beans and at least 60 plants of dwarf beans, divided into 2 replicates.
6. 3. 2 Field management
The management measures of the test field are basically the same as those of the bean production field. The field fertilization of the medium variety, similar varieties and standard varieties should be strictly consistent.
6.4 Observation of traits
6.4.1 Observation period
Observation of traits shall be carried out according to the growth stages listed in Table A.1 and Table A.2. The description of growth stages is shown in Table B.1.6.4.2 Observation method
Observation of traits shall be carried out according to the observation methods (VG, VS, MG, MS) specified in A.1 and Table A.2. 6.4.3 Number of observations
Unless otherwise specified, the number of samples for individual trait observation (VS, MS) shall not be less than 20. When observing the organs or parts of plants, the number of samples for each plant shall be 1. For group observation of traits (VG, MG), the whole plot or a mixed sample of a specified size shall be observed.5 Additional tests
When necessary, additional tests may be performed using the traits listed in Table A, 2 or traits not listed in this document. 7 Determination of specificity, consistency and stability results 7.1 General principles
Specificity, consistency and stability shall be determined in accordance with the requirements of GB/T19557.1 7.2 Determination of specificity
NY/T 2427—2013
The applied variety shall be clearly distinguished from all known varieties. In the test, when the applied variety has a significant and reproducible difference from similar varieties in at least one trait, the applied variety can be determined to have specificity. 7.3 Determination of consistency
The determination of consistency adopts a population standard of 1% and a probability of acceptance of at least 95%. When the sample size is 60 strains, a maximum of 2 atypical strains are allowed; when the sample size is 150 strains, a maximum of 4 atypical strains are allowed. 7.4 Determination of stability
If a variety has consistency, it can be considered to have stability. Generally, stability is not tested. If necessary, the next generation of the variety can be planted. If there is no obvious change in the expression of the trait compared with the previously supplied propagation material, the variety can be determined to be stable.
8 Trait table
According to the test needs, the traits are divided into basic traits and selected traits. Basic traits are traits that must be used in the test. The basic traits of beans are shown in Table A.1, and the selected traits are shown in Table A.2. 8.1 Overview
The trait table lists the trait name, expression type, expression state and corresponding code and standard variety, observation period and method, etc. 8.2 Expression type:
According to the trait expression method, the traits are divided into three types: quality traits, pseudo-quality traits and quantitative traits. 8.3 Expression states and corresponding codes
8.3.1 Each trait is divided into a series of expression states to facilitate the definition of traits and standardized descriptions; each expression state is assigned a corresponding digital code to facilitate data recording, processing and the establishment and communication of variety descriptions. 8.3.2 For quality traits and pseudo-quality traits, all expression states should be listed in the test guide; for number traits, in order to shorten the length of the trait table, the expression states of the number codes may not be listed, and the expression states of the even codes may be described in the form of the previous expression state to the next expression state.
8.4 Standard varieties
The trait table lists the standard varieties that can be used as reference for the expression states of some traits to help determine the different expression states of the relevant traits and correct for differences in environmental factors.
9 Grouped traits
In this document, the variety grouping traits are as follows: a) * Plant: Growth type (Character 3 in Table A.1). b) * Flower: Petal color (Character 17 in Table A.1). c) * Flower: Base color (Character 22 in Table A.1). d) * Flower: Cross-section shape (Character 34 in Table A.1). c): Texture type (Table A.1 Neutral trait 35) f) Seed coat: Number of colors (Table A.1 Neutral trait 42). g) * Seed coat: Primary color (Table A.1 Neutral trait 43). 10 Technical Questionnaire
The applicant shall fill in the technical questionnaire of kidney beans in the format given in Appendix C. NY/T 2427—2013
A.1 Basic traits of kidney beans
See Table A. 1.
Serial number!
Plant: Hypocotyl anthocyanin color
Plant special: Hypocotyl anthocyanin test color intensity
+Plant: Growth type
Applicable to basal types of varieties: Starting period of vine emergence (80% of plants have vine emergence)
Applicable only to dwarf types of varieties: Vine emergence speed QN
Applicable to dwarf types of varieties: Plant: Morphology
Applicable only to dwarf types of varieties: Plant: Height
*Leaf: Green degree
Leaf: Leaf surface degree
Terminal leaflet: Size
Terminal leaflet: Color
Native subtotal, leaf tip length
Appendix A
(Normative Appendix]
Bean trait table
Table A. 1 Table of basic characteristics of kidney beans
Observation period
and method
22~-33
Expression state
Triangle
Ovate
Standard elegant varieties
Purple bean (C)
Supplier (D)
Purple bean (C)
Supplier (D)
Bifeng (C)
Bifeng (C)
White flower rack bean ()
Mengyin, Qiubulao; Meidou (C)
Yellow Seed beans (C)
Feng (C)
Supplier (D)
White sheep horn (D)
C-BR-18 (D:
Supplier (D)
Purple horn beans (D)
Hebei Jinjia oil beans (C)
Shuangfeng 2 (C)
Yellow seed beans (C)
Xi Ji beans (（)
351 (C)
Golden diesel beans (C)
Shuangfeng 2 (C)
Bifeng (C)|| tt||Flowering period (50% of the plants have at least seven flowers
Applies only to dwarf varieties; inflorescence; position
Flower: bract size
*Flower · Flag color
*Flower color
This applies to creeping varieties: Plant type QL
*This applies to dwarf American varieties: English: Length
(excluding Xi)
*Applies only to American varieties of English: Length
(excluding Xi)
English: Width||tt ||Pod; thickness-width ratio
*英:基他
英:基色强度
*英:斑纹
*莱·斑纹色
Table A.1 (continued)
Observation period
and method
33~-39
33--39
Expression status
Leaf from the Rui
Intermediate type
Outside the leaf cluster
Pink-white
Pink-white
Rectangular
NY/T 2427—2013
Standard variety
86-2(D)
Shuangfeng 2(C℃)
Bifeng(C)
Big flower sparrow egg ()
351(C)
(GBR-18(ID)
Green peel(C)
Super long string beans(C)
Bifeng(()
High yield beans(C)
Red arm pump (D)
Purple beans (C)
Bifeng (C)
# Bean production (C)
Red kidney beans (D)
Purple beans ()
Super long four-season hat
Dwarf golden hook ((D)
Red beans (D)
Wide Ying white lotus (D)
Golden hook (C)
High-yield beans (C)
Bifeng ()
Super Long season beans (C)
Supplier (D)
Kuan Ying Bai Lian (D)
Shan+ (C)
CC-BB-18 (D)
Super long season beans (C)
Golden hook (C)
Lu Feng (C)
Purple horn beans (D)
NY/T2427—2013
Lai: Strength of pattern color
*Class: Suture Red vegetative
英: curvature
英: curvature shape
fluorescence: end shape (not included)
* vegetable: Xi length
pod: beak curvature
type: surface texture
×英: section shape
兼: texture type
: shrinkage (ten dry)
card seed; 100-grain weight
种了: longitudinal section shape
Table A.1 (continued)www.bzxz.net
Observation period
and method
Expression state
None or very weak
Sharp to blunt
Smooth or slightly rough
Moderately rough
Oval
Oval
8 Shape
None or polar
Round to tree round
Round
Standard variety
Purple horn ()
Red kidney bean ()|| tt||Concave flower kidney beans (C)
H-ben flower face kidney beans (C
Super long four seasons (C)
Green peel (C)
Bifeng()
Super long green beans (C)
Super long green beans ()
Red kidney beans (D)
Bifeng (heart)
Provider (D)
Double car 2()
White flower frame beans (C)
Seven-inch big bend()
Bifeng ( C)
Red bean width (C)
This applies to kidney-shaped seeds in longitudinal section; curvature QN
seed: cross-section shape
seed: cross-section width
*Seed coat: number of quivering
*Seed coat: primary color
Seed coat: dark color
Seed coat: secondary color distribution
Seed coat: stripe shape
Seed coat: vein pattern
seed·umbilical ring color
Selected traits of kidney beans
See Table A.2.
Table 4.1 (continued)
Observation period
and method
Expression status
Observation ellipse
Medium ellipse
2 more types
Brown yellow
Brown yellow
Around the hilum
Half a seed
Slightly a seed
Different from the seed coat color of the crumbs
Same as the seed coat color of the surrounding
NY/T 2427—2013
Standard Varieties
Extra Long Green Beans (C)
Red Kidney Beans (D)
Shuangyang Large Flower Peel (C)
Xi Ji Beans (C)
Bifeng (C)
Purple Corn Beans (D)
Red Beans Wide (C)
Black Beans (C)
NY/T 2427—2013
! White seed coat varieties: color of mature grains (401 days early)
Table A.2 Table of selected traits of kidney beans
Observation period
and method
Dead state
Light green
Standard variety
Bifeng (C)
CX-BB-18D)
B.1 Kidney bean growth stage
See Table B.1.
Main growth stage code
Explanation of multiple traits
Appendix B
(Normative Appendix)
Explanation of bean trait table
The first pair of true leaves unfold
Bean growth stage table
For creeping varieties, the upper end of the main stem creeps and begins to twine. About 50% of the plants have at least one flower in bloom
At full bloom, all plants have bloomed
Middle stage of pod elongation and swelling (pod length and width are about 1/2 of the maximum value) The pod fully elongates and swells and reaches the maximum length and width (fruit green maturity stage) The pod physiologically matures and dries
Seeds are dried and harvested
(a) Leaves: Observe the functional leaves in the middle of the plant during the full bloom period. B.3 Explanation of individual traits
See Table A.1 for trait classification and codes in the figure.
MY/T2427—2013
Character 5 is only applicable to vine-growing varieties: vine-growing speed, the number of days from the first pair of true leaves to the plant height of 1.5m. Character 9 Leaf: the degree of leaf surface convexity, see Figure B1. Cut
Leaf: the degree of leaf surface convexity
Character 11 Terminal leaflet: shape, see Figure B.2. Strong
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