Some standard content:
JJG537-2006
Verification Regulation of
Fluoreycence SpectropbotometerJ3G537—20UG
代沫JJG537—1988
JJG536—1986
This regulation was approved by the General Administration of Quality Supervision, Inspection and Quarantine on May 23, 2006, and came into effect on November 23, 2006.
Responsible unit: National Physical and Chemical Metrology Technical Committee Drafting unit: China National Institute of Metrology Drafting unit: 1 Haisan Instrument Co., Ltd. This regulation is entrusted to the National Physical and Chemical Metrology Technical Committee for interpretation and use
3 Meter* Performance requirements
The indication value is set to be stable
.2 Inspection limit.
3.3 Measurement line ratio
3.4 Fluorescence peak 3.5 Sequence of values 4 Technical requirements 4.1 External and initial inspection 4.2 Insulation core twist 5.2 Measuring instrument and its control 5.3 Verification method 6.1 Treatment of long-term results 7.2 Determination of the thickness of the plate 8.3 Determination of the thickness of the plate 9.4 Determination of the thickness of the plate 10.5 Determination of the thickness of the plate 11.6 Determination of the thickness of the plate 12.7 Determination of the thickness of the plate 13.8 Determination of the thickness of the plate 14.9 Determination of the thickness of the plate 15.10 Determination of the thickness of the plate 16.11 Determination of the thickness of the plate 17.12 Determination of the thickness of the plate 18.13 Determination of the thickness of the plate 19.14 Determination of the thickness of the plate 19.15 537—200%
.-. (1)
TTWn.-T27.
.......
........
Appendix VIII Preparation of standard solution for calibration of the micro-lamp line
Measurement instrument for the light-emitting device of the factory
Appendix II Expansion formula
Attachment: Format for recording the data of the light spectrophotometer - Time recording E-gun format
. (3)
: (12)
1 Range
JC S37-286
Verification Procedure for Fluorescence Spectrophotometer
Prepare for the verification of fluorescence spectrophotometer, and continue to verify the inspection in use. 2. Some substances will emit light with a longer wavelength than the irradiation wavelength after being excited by the irradiation of light. This light is called fluorescence. The relationship between the intensity of the radiant light and the energy of the substance is as follows: F=k'@l, tl-em)
formula:
Light emission:
Instrument efficiency;
Micro-radiant intensity:
=-—gram The intensity of the radiant material is: The intensity of the radiant material in the layer is:
The intensity of the radiant material required:
Energy efficiency of the radiant material:
For a given substance, when the length and intensity of the covering layer are fixed, the original pressure of the liquid layer is fixed, and the concentration of the falling liquid is low, the intensity of the radiant light and the energy of the radiant material have the following relationship: y-ke
For sale optical spectrophotometer (the instrument below) According to the above principle, the material that can radiate radiant light can be qualitatively analyzed by walking, and the amount is analyzed by the instrument.
The instrument mainly consists of light source, excitation detector system, sample chamber, emission monochromator, dynamic imaging system and data processing system.
The end of the detector can be divided into two categories: Category A is the monochromator with filter. Category B is the filter detector. 3 Metrological performance requirements 3.1 The wavelength indication and value determination of type A monochromator shall meet the following requirements: wavelength indication multiple difference limit: optimal 112.0am; wavelength tolerance: 1.0mm; 3.1.2 The light transmission characteristics of type R monochromator filters: nominal value -10mm+1mm; laser filter: nominal value +5m; 3.2 Detection limit S37-204
Using sulfuric acid to control the single mountain limit of the chemical research: the instrument with a class A monochromator has a detection limit of S/10-\giml. The instrument with a class A monochromator has a detection limit of "g one receiving plate, etc. 1 and two class A colors are configured at the same time, and the index of the monochromator is 13.3 The most linear
instrument is only 8 small fire results r0.995
must be a straight-hearted instrument, but the accuracy of the general indicator is less than 0.5 level, this item can not be easily determined. 3.4 Fluorescence light library value intensity
drink The instrument measures the optical spectrum with an average internal weight of 1.5%. 3.5.1 0.5% is required. 3.5.2 The upper limit of the optical blood high indication value is within 10min and does not pass the standard = 1.5%. General technical requirements. 4.1.1 The receiver is marked with the following: receiver, model, manufacturer name, mark, factory time and instrument serial number. 4.1. All the parts of the instrument should be in good condition, all the switches and buttons can stop working, and the electrical wires should be in good condition. 4.1.3 The indication of the instrument should be normal, the lines should be clear and evenly spaced: the width of the potential meter should not be too wide, the half-width and half-width should be straight, and the meter should be stable and without any jumps when the "adjustment" and "full room" indication of the instrument is on. 4.1.5 The filter components of the receiver should not contain any secondary oil or other points that may affect the communication. 4.3 When the receiver is in the working state and the test voltage is 500V, the insulation resistance between the power supply line and the shell is less than 20 n
5 Measuring instrument control
Chuanzhi and other control only before the heart: first drink set, then involved in the stable enterprise use age test, 5.1 Verification policy
5.1.1 Environmental conditions
Environmental theft (2) = 1): relatively stable:
* humidity small people 85%:
L electricity (+22
5.12 Clean equipment
TO-535—2046
Working standard Yanyou battery and its distribution transformer difference should not be more than 0.5%, UV-dispersible light tiger meter: plated length indication Baolan Wang 1.mm stopwatch minute low host 0.
AC heart table: 15 Yangtong H10 level
megohmmeter! Test delivery 500V.
Preparation of international 5.1.3. Standard material for air-resistant operation, national standard material 5.13.2.1.4. National standard material 5.13.2.1.4. National standard material 5.12.10.05.5.12.2.10-91×10.5.2.2.3. Technical standard material 5.1.3.1.4. National standard material 5.13.2.1.4. National standard material 5.13.2.1.4. National standard material 5.13.2.1.4. National standard material 5.13.2.1.10.05.5.12.2.10-91×10.5.2.2.2. Technical standard material 5.13.2.10-91×10.5 ...10-91×10.5.2.2.10-91×10.5.2.2.10-91×10.5.2.2.10-91×10.5.2.2.10-91×10.5.2.2.10-91×10.5.2.2.10-91×10.5.2.2.10-91×10.5.2 Invest in
standard liquid, its quality is divided into:
high leather shop
AANETRO
not on behalf of Japan
Xi'an City Test
2 party dinner and note Yili area Hui University teaching optics and service ice party release industry second public Beibei national planning line 5 inspection stop party encounter
teaching preparation should be formed in the working atmosphere, passive no direct on the instrument night whole same country light love field, day field ten this, free or: high frequency gas just contact consultation, standard only served 20m, early and natural light cow instrument you, Bi Le An wash female natural light film, more you quickly open charge cuts electric products 5.3.1 appearance and preliminary inspection three according to 4.1 fee request line JG537-2006
5.3.27 type single other receiver wave value error and long repeatability 5.3.2.1 Method for the bright end of the lamp
, the method for the bright end of the lamp is to use a type of () excitation monochromator. If the wavelength indication value is different from the measured monochromator, the sample is placed in an empty reflector (or white filter paper without glare). Set the self-reflection time of the device to a fast speed, set the scanning speed to "medium" or "low". Use a practical measurement vehicle to scan the entire emission side monochromator in the range of (350-550) m. Find the spectrum near 450.1 mm in Figure 1 and determine its peak position. For the third measurement, the formulas (1) and (2) are used to calculate the desired wavelength indication error and the measurement wavelength indication error respectively:
a, -.(um)
Test value:
5:—Test value is low (hydrogen lamp charging age test wavelength peak value: 4.50.1mm) wavelength sub-base:
(b) Emission data monochromator wavelength constant error and wavelength repeatability will be placed in the special position of the transmitter, the source reflection alarm (or no light source gate) is effective in the sample room, the time of the changer can be set to "absent", the scanning speed is strictly set to "medium" or manual mode, so that the actual number of months is abandoned. The length range of the transmitter (350-55U) is cleared, and the light peak near 45).1m is found on the obtained port map, and its rate value position is determined. Continuously measure twice, and calculate the wavelength indication error and reproducibility according to formulas (1) and (2). Three: For a wet industry, the transmitter is of course related to the repeatability of the measurement, and the scientific method is used to increase the benefit of the method, which is the method B or only for the purpose of explanation. 5.3.2.2 Position
() Excitation measurement monochromator wavelength error and wavelength repeatability Each emission time setting: 331m, the sample room needs to have a luminescent sample of alcohol (1×10-g/ml), the required time period should be set to "fast", the scanning speed should be set to "medium" or manual, the actual feasible degree (1~3)Tm, for the wavelength range of the micro-emitting monochromator (240-35)) um, the sample room needs to have a luminescent sample of alcohol (1×10-g/ml), the sample room needs to have a luminescent sample of alcohol (1×10-g/ml), the sampling time period should be set to "fast", the scanning speed should be set to "medium" or manual, the actual feasible degree (1~3)Tm, for the micro-emitting monochromator (240-35)) um, ... S3T2HG
Introduce the sample pen, the receiver should be set to "fast", the scanning speed should be "medium" or dynamic mode, use a narrow chain width seat (1.-3)smL to draw the emission monochromator (240-400)mm rejection graph: 1: Find the light increase and decrease on the obtained harmonic function, and determine its peak position. Measure three times in a row and calculate the error of the indication value according to formulas (1) and (2) respectively. 5.3.3 Verification of monochromator speed filter
3.3.3.1 Optical characteristics of bandpass filter. Use a visible-light spectrophotometer to measure the transmittance of the light at each wavelength of the receiver. Continue to make a comparison of the performance characteristics (Ni-factor) and find the minimum maximum wavelength and the transmittance of the wavelength corresponding to the input wavelength. The wavelength error of the monochromator solution is: =-
Where: -
- ... From 50m, the needle reported 450 right in the original period, set the two seen breaking length or make the selection method of light blank bullet is very consistent with the standard stealing world bank continuous exchange belt modification certificate, non-if in the training quantity this 1 time teaching sales recognition quantity out of ten jobs to start compared with the twisted large people orchid, the output guide set up number training brush addition use the true time measurement full change light intensity:
Wu Zhong F
indicates the disease light flux of the added liquid
blank solution of the home light flux
light Zhang Hu estimated the average value of the production
national capital
AOEOMHSE
correct service Yitang light
change out authorized limit index section 2
Wu Zhong: production
11 welcome flat purchase light intensity
standard and use the quality information
a single book standard has a healthy difference.
3.3.5 According to the natural production
month 0.05ol reduction as the white density liquid
each quality energy newly established production
Beijing rules, set the measurable length
standard as the comprehensive zero ()
standard standard concentration ()
quality concentration, use cold
super Sheng Mo Ling mirror
block and light system,
before measuring
install the length below Table 2
install the length let
polystyrene ball produce Ning guide standard hidden glue
no technology 45℃ left
respectively, measure the kinds of quantities in Table 2 as standard dissolved in the air for two times, calculate the average light intensity of each quantity, use the small two method, the kinds of standard sent you medical and fluorescent you health product length The supply is carried out to deal with the relevant effects of the stove, that is, the measurement line is produced by the calibration result (the quantity is small three this public child attached system
53.6 the light rate value of the old house is the main complex
vision store is naturally the test length is 350, the emission length is reduced by 45 on the right principle, the length on both sides selects HG537-2
You my film, the electric 1 Yi 10 air treasure stops, see no 9m, to emit from 5 air SO five virtues scan once high record the signal light obstacle value the main female middle and lower type meter bullet: ax
Wu Zhong: F
Open the signal intensity setting
SOXUONITN
5.3.7 according to the fixed current
S3.71 item single # installation, close the idle charging door, record the original shift within. 5.3.7.2 The excitation pool is specially designed for the wavelength of 450. The micro-transmitter and the transmitting end are as large as 1Dm. No single product room is allowed to be placed in the radiation board, S.3.8 The new version of the household appliance is used to make the power supply of the household appliance change. The electronic meter of the instrument is connected to the power supply of 500V. The user needs to apply 5D power between the user and the external charger to test the whole voltage. After 5 seconds, the power group is used. 5.3.0 The typical version of the installation model is more dangerous! The main technical indicators of the actual situation will be determined by the scientific and technological party next year. The most typical record can be more. 5.4 The results of the auction must be processed according to the requirements. The relevant information should be completed or indicated in the verification certificate. 5.4.2 The light inspection and dangerous level inspection process must comply with the regulations of the road protection department. If it violates the regulations, the project will not be recruited by the Shenzhen Municipal Station!
5.5 Calibration materials
Please use the correct format for the book:
Book (# determination
Full determination
The format is the same as the record F,
The calibration period is 1 year, before the promotion
When the parent material changes and the source card is replaced, the light gate stops changing and the top 4 lines of EETRE
Self-test card has love.
Appendix A
A.1 Reagents
World famous teachers
Chemical formula
Relative molecular weight
A-2 Micrometer
JJG S37—20I6
Preparation of working standard solutions for calibration
Table A.1 Trial and standard substances
(CH,ON-H,SO, -2H,O
Network Beijing has no standard material
Use burette, colorimeter tube, non-dividing inlet tube, and scientific quantity grain measurement and calibration. Table 3.2 World standard
Natrium qualitative
Special medium
Special medium
Special medium (1.01mg
A.30.UsmolL flow sieve preparation
Single standard pipette
No, 250, t oHM m1.
1:10.25 uL
Add 100mL of the solution to the hot water, use a titrator to take a concentration of 2.7uL and add it to the volume, use the distilled water to make up to the full scale, mix well and put aside for later use. A.4 Preparation of standard solution of anti-inflammatories
.4.1 Add 500mL of 1×10^gluL of succinic acid. Place the solid reagent of succinic acid in a desiccator for 24 hours. Weigh 5.00% of the standard substance of anti-inflammatories in 5mL of water on a balance. Add an appropriate amount of 0.05M sulfuric acid solution. Dissolve it, then carefully dilute to the scale line, and mix evenly. A.4.2 Example 25 (mT.1×10*mL porcelain ningbottle method Use a single volume of 25m110:g ningbottle acid, add 250 (.05wa/potency acid and the like to the scale line, and mix evenly. S
JG537—2006
几.4. Prepare other concentrations of ningbottle standard specifications by analogy. The volumes and concentrations of the standards tested are shown in Table 3. For the sake of clarity, 1 person 10-mJ.The standard liquid is also listed in the table: Table 3 Standard liquid for alcohol and charin standard method for matching standard language energy
quality (L)
1×10*
4×10**
K × In-*
1210-t
1×10-
Ix 10-
1×1n-
The standard liquid is
A.4.4 Store in a dark glass bottle with high temperature and standard increase policy. Remove the light at class. Keep it in a sealed place: Replace it with the standard one every week. The charging period is half a year. B.1 When the monochromator is self-discharging, the loss of the wave is required. The monochromator should be specially made for the emission of zero-level price. Correctly install the single-type lamp on the product shelf in the home. Use the most practical and feasible one. To hold the right to strive for the special standard, allow from the middle of the country to start the strange attention, from the return wave to the long silver to Liu hair to the single device seedling. Electric to change has been relying on the most technical heaven country several inches of the design before, engineering records are secondary, according to the reason support (mountain.1) benefit (B.2) respectively feder long show letter class difference straight Beijing pieces, R
micro long chapter position
people—science is long value.
R.2 mercury you test
low music spirit on the language surface of the physical grid can be related to the friend's instrument reason degree B. day's full certificatewwW.bzxz.Net
table use.1 low voltage to draw the light world more test happy long travel device
ping tenure product has table
push strong class
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