This standard specifies the use of spectrophotometry to test the content of polyethylene glycol or protein of different molecular weights in a solution, calculate the retention rate, estimate the pore size of the hollow fiber membrane, and confirm the performance of the hollow fiber ultrafiltration membrane. This standard is applicable to the performance test of hollow fiber ultrafiltration membranes made of different materials. HY/T 050-1999 Hollow Fiber Ultrafiltration Membrane Test Method HY/T050-1999 Standard Download Unzip Password: www.bzxz.net
This standard specifies the use of spectrophotometry to test the content of polyethylene glycol or protein of different molecular weights in a solution, calculate the retention rate, estimate the pore size of the hollow fiber membrane, and confirm the performance of the hollow fiber ultrafiltration membrane. This standard is applicable to the performance test of hollow fiber ultrafiltration membranes made of different materials.
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ICS.07.060 Marine Industry Standard of the People's Republic of China HY/T 050—1999 Test methods for hollow fiber ultrafiltration membranes Test methods for hollow fiber ultrafiltration membranesPublished on April 26, 1999 Published by the State Oceanic Administration Implemented on July 1, 1999 HY/T050--1999 This standard refers to ASTM E1343:1990 "Test method for evaluation of ultrafiltration membranes by molecular weight cutoff method", ASTM D5090:1990 "Standardized test data for ultrafiltration permeation flow rate" of the American Society for Testing and Materials and Japanese Industrial Standard JIS K3821--1990 "Test method for pure water permeability of ultrafiltration components", JIS K3802-1989 "Terminology of membrane and membrane process". Appendix A of this standard is the standard appendix, and Appendix B is the suggested appendix. This standard was proposed by Tianjin Textile Engineering College. This standard is under the jurisdiction of the National Marine Standard and Metrology Center. The drafting unit of this standard is Tianjin Textile Engineering College. The main drafters of this standard are Wei Jianmin, Zhang Huixin, Dai Haiping, and Zhao Changsheng. This standard is entrusted to Tianjin Textile Engineering College for interpretation. 1 Scope Marine Industry Standard of the People's Republic of China Test methods for hollow fiber ultrafiltration membranes Test methods for hollow fiber ultrafiltration membranesHY/T050—1999 This standard specifies the use of spectrophotometry to test the content of polyethylene glycol or protein of different molecular weights in the solution, calculate the retention rate, estimate the pore size of the hollow fiber membrane, and confirm the performance of the hollow fiber ultrafiltration membrane. This standard is applicable to the performance test of hollow fiber ultrafiltration membranes of different materials. 2 Terms This standard adopts the following definitions. 2.1 Pure water permeability purewaterflux Under certain flow rate, temperature and pressure, the amount of pure water permeated by the ultrafiltration membrane per unit time and per unit membrane area. 2.2 Rejection The ratio of specific solutes retained by the membrane to the total specific solutes in the solution. 2.3 Retention molecular weight retentionmolecularweight Under certain conditions, substances of certain molecular weights are retained by the membrane. The minimum molecular weight of the retained substance is the membrane’s retention molecular weight, which is used to characterize the membrane’s separation ability. 3 Test method 3.1 Test of pure water permeability Under operating pressure of 0.1MPa and room temperature, test the amount of pure water permeated per unit time and per unit membrane area. 3.2 Polyethylene glycol content test Polyethylene glycol and Dragendoff reagent can generate an orange-red complex, and the polyethylene glycol content in the solution is tested by spectrophotometry. Add polyethylene glycol of known molecular weight to water and pass it through the ultrafiltration membrane. Test the change in the concentration of polyethylene glycol solution before and after ultrafiltration, and calculate the retention rate of the hollow fiber ultrafiltration membrane for polyethylene glycol of this molecular weight. 3.3 Protein content test Prepare protein solutions of different molecular weights, and use ultraviolet spectrophotometry to test the change in the concentration of the protein solution before and after passing through the ultrafiltration membrane, and calculate the retention rate of the hollow fiber ultrafiltration membrane for proteins of different molecular weights. 4 Main reagents and materials - Distilled water or water of equivalent purity; Polyethylene glycol: molecular weight 6000, 10000, 20000 imported packaging; bismuth subnitrate [4BiNO:(OH),BiO(OH)J: analytical grade; glacial acetic acid (CH.COOH): analytical grade; Potassium iodide (KI): analytical grade; Approved by the State Oceanic Administration on April 26, 1999 Implemented on July 1, 1999 HY/T 050—1999 Sodium acetate (CHCOONa·3H,O): analytical grade; cytochrome C (molecular weight 13000): freeze-dried preparation; ovalbumin (molecular weight 45000): egg protein tablets; bovine serum albumin (molecular weight 67000): biochemical reagent. 5 Instruments and equipment Ultraviolet spectrophotometer; Photoelectric spectrophotometer; Analytical balance; A vacuum drying oven; Dryer; Volume flask, pipette, beaker, etc. 6 Schematic diagram of ultrafiltration membrane test device The schematic diagram of ultrafiltration membrane test device is shown in Appendix A (Standard Appendix). 7 Test steps 7.1 Test of pure water transmittance 7.1.1 The test method of pure water transmittance is the same as 3.1. 7.1.2 Calculation of pure water transmittance Where: F- Pure water transmittance, m/m2.h); Pure water transmittance, m; Membrane area, m2; -Time used to collect pure water transmittance, h. 7.1.3 Calculation of membrane area A = naDL Wherein; A Membrane area, m; Number of hollow fiber membranes; D--diameter of hollow fiber membrane (inner diameter for inner pressure membrane, outer diameter for outer pressure membrane), m; L--effective length of hollow fiber membrane, m. 7.2 Test of polyethylene glycol content 7.2.1 Preparation of reagents (1) 7.2.1.1 Preparation of Dragendoff reagent Solution A: Accurately weigh 0.800g of bismuth subnitrate and place it in a 50mL volumetric flask, add 10mL of glacial acetic acid, and then dilute to the mark with distilled water. Solution B: Accurately weigh 20.000g of potassium iodide and place it in a 50mL brown volumetric flask, and dilute to the mark with distilled water. Dragendoff reagent: Measure 5 mL of solution A and solution B and place them in a 100 mL brown volumetric flask, add 40 mL of glacial acetic acid, and then dilute to the mark with distilled water. Its validity period is half a year. 7.2.1.2 Preparation of acetic acid-sodium acetate buffer Measure 590 mL of 0.2 mol/L sodium acetate solution and 410 mL of 0.2 mol/L glacial acetic acid solution and place them in a 1000 mL volumetric flask to prepare pH 4.8 acetic acid-sodium acetate buffer. 2 7.2.2 Preparation of standard solution HY/T050—1999 Polyethylene glycol is placed in a vacuum drying oven at 60°C and dried for 4 hours to remove moisture. Accurately weigh 1.000g of polyethylene glycol and dissolve it in a 1000ml volumetric flask. Take 0, 0.5, 1.0, 1.5, 2.0, 2.5, and 3.0mL of polyethylene glycol solution and dilute them in a 100mL volumetric flask to prepare polyethylene glycol standard solutions with concentrations of 0, 5, 10, 15, 20, 25, and 30mg/L. 7.2.3 Preparation of standard curve Take 5mL of the standard solutions of different concentrations in 7.2.2 and place them in 10mL volumetric flasks. Add 1mL of Dragendoff reagent and 1mL of acetic acid-sodium acetate buffer respectively, and dilute to the scale with distilled water. After standing for 15 minutes, measure the optical density on a photoelectric spectrophotometer at a wavelength of 510nm using 1cm colorimetric blood, with distilled water as the blank. Plot the standard curve with polyethylene glycol concentration as the horizontal axis and optical density as the vertical axis. 7.2.4 Sample testing 7.2.4.1 Preparation of sample solution Select polyethylene glycol of a certain molecular weight; prepare a polyethylene glycol solution with a concentration of 5000 mg/L, which is used as the solution for evaluating the performance of hollow fiber ultrafiltration membrane. 7.2.4.2 Evaluation of hollow fiber ultrafiltration membrane The prepared sample solution is run through the hollow fiber ultrafiltration membrane at 0.1 MPa and room temperature, and the ultrafiltrate is collected after 20 minutes. After the stock solution and the ultrafiltrate are diluted by appropriate times, their optical density is determined, and the corresponding concentration is obtained from the standard curve. 7.2.5 Calculation of retention rate Ru = 2 × 100 Where: Ru——-Retention rate, % -Polyethylene glycol concentration in the stock solution, mg/L; C2—Polyethylene glycol concentration in the permeate, mg/L. 7.3 Protein content test 7.3.1 Preparation of standard solution (3) Bovine serum albumin (BSA) was vacuum dried at 105°C to constant weight. Accurately weigh 1.000 g BSA and dissolve it in a 1000 mL volumetric flask. Pipette 0.2, 0.4, 0.6, 0.8, and 1.0 mL of BSA solution into a 10 mL volumetric flask, add distilled water to dilute to the mark, and prepare concentrations of 20, 40, and 60.80, 100 mg/L bovine serum albumin standard solution. 7.3.2 Preparation of standard curve Protein has maximum absorption of ultraviolet light at 280nm. The absorption value of protein solution at 280nm is proportional to its concentration. Measure the optical density of the standard solution in 7.3.1 on a UV spectrophotometer at a wavelength of 280nm using 1cm colorimetric blood, and use distilled water as a blank. Plot the protein concentration as the horizontal axis and the optical density as the vertical axis to prepare a standard curve. 7.3.3 Sample testing 7.3.3.1 Preparation of sample solution Select a protein of a certain molecular weight and prepare a protein solution with a concentration of 1000mg/L to 3000mg/L. The egg white protein solution is used after centrifugation or filtration. The prepared protein solution is used as a solution for evaluating the performance of hollow fiber ultrafiltration membranes. 7.3.3.2 Evaluation of hollow fiber ultrafiltration membrane The prepared sample solution was run through a hollow fiber ultrafiltration membrane at 0.1MPa and room temperature, and the ultrafiltrate was collected after 20 minutes. The optical density of the stock solution and the ultrafiltrate was measured in the 280nm ultraviolet region, and the corresponding concentration was obtained from the standard curve. 7.3.4 Calculation of interception rate The calculation formula of the interception rate is the same as formula (3) in 7.2.5. 8 Test resultsWww.bzxZ.net Two samples were taken from each sample for parallel testing, and the arithmetic mean of their test values was taken as the test result. 3 Test report The test report shall include the following contents: HY/T050—1999 a) Type, specification, batch number and manufacturer name of the test membrane sample; b) Room temperature and liquid temperature during the test; c) Test results; d) Observation of phenomena during the test; e) Test date; f) Tester. The schematic diagram of the ultrafiltration membrane test device is shown in Figure A1. HY/T050—1999 Appendix A (Standard Appendix) Ultrafiltration membrane test device 1—Water tank; 2—Pump 3—Pressure gauge: 4-Ultrafiltration membrane assembly; 5—Valve Ultrafiltration membrane test device and schematic diagram Appendix B (Suggestive Appendix) References ASTME1343:1990 ASTMD 5090:1990 3JISK3821:1990 JISK3802:1989 Test method for evaluating ultrafiltration membranes by molecular weight cutoff method Standard test data for ultrafiltration permeation rate Test method for pure water permeability of ultrafiltration components Terminology of membranes and membrane processes HY/T050-1999 Marine Industry standard of the People's Republic of China Hollow fiber ultrafiltration Filter membrane test method HY/T050—1999 Published by China Standards Press No.16, Sanlihebei Street, Fuxingmenwai, Beijing Postal code: 100045 Tel: 6852394668517548 Printed by Qinhuangdao Printing Factory of China Standards Press, distributed by Xinhua Bookstore in Beijing, sold by Xinhua Bookstores in various places* Word count: 12,000 words||tt| |Printing sheet 3/4 Format 880×1230 First printing in July 2002 First edition in July 2002 Print run 1-500 Website www.bzcbs.com Subjects 610—683 Copyright reserved Infringements must be investigated Report phone number: (010)68533533 —0SO L/XH Tip: This standard content only shows part of the intercepted content of the complete standard. 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