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Test methods for hollow fiber ultrafiltration membranes

Basic Information

Standard ID: HY/T 050-1999

Standard Name:Test methods for hollow fiber ultrafiltration membranes

Chinese Name: 中空纤维超滤膜测试方法

Standard category:Marine Industry Standard (HY)

state:in force

Date of Release1999-04-26

Date of Implementation:1999-07-01

standard classification number

Standard ICS number:Mathematics, Natural Sciences >> 07.060 Geology, Meteorology, Hydrology

Standard Classification Number:Comprehensive>>Basic Subjects>>A45 Oceanography

associated standards

alternative situation:State Oceanic Administration

Procurement status:ASTM E1343-1990 NEQ ASTM D5090-1990 NEQ JIS K3821-1990 NEQ JIS K3802-1989 NEQ

Publication information

publishing house:China Standards Press

ISBN:155066.2-14495

Publication date:1999-07-01

other information

drafter:Wei Jianmin, Zhang Huixin, Dai Haiping, Zhao Changsheng

Drafting unit:Tianjin Textile Institute

Focal point unit:National Marine Standards and Metrology Center

Proposing unit:Tianjin Textile Institute

Publishing department:State Oceanic Administration

competent authority:State Oceanic Administration

Introduction to standards:

This standard specifies the use of spectrophotometry to test the content of polyethylene glycol or protein of different molecular weights in a solution, calculate the retention rate, estimate the pore size of the hollow fiber membrane, and confirm the performance of the hollow fiber ultrafiltration membrane. This standard is applicable to the performance test of hollow fiber ultrafiltration membranes made of different materials. HY/T 050-1999 Hollow Fiber Ultrafiltration Membrane Test Method HY/T050-1999 Standard Download Unzip Password: www.bzxz.net
This standard specifies the use of spectrophotometry to test the content of polyethylene glycol or protein of different molecular weights in a solution, calculate the retention rate, estimate the pore size of the hollow fiber membrane, and confirm the performance of the hollow fiber ultrafiltration membrane. This standard is applicable to the performance test of hollow fiber ultrafiltration membranes made of different materials.


Some standard content:

ICS.07.060
Marine Industry Standard of the People's Republic of China
HY/T 050—1999
Test methods for hollow fiber ultrafiltration membranes
Test methods for hollow fiber ultrafiltration membranesPublished on April 26, 1999
Published by the State Oceanic Administration
Implemented on July 1, 1999
HY/T050--1999
This standard refers to ASTM E1343:1990 "Test method for evaluation of ultrafiltration membranes by molecular weight cutoff method", ASTM D5090:1990 "Standardized test data for ultrafiltration permeation flow rate" of the American Society for Testing and Materials and Japanese Industrial Standard JIS K3821--1990 "Test method for pure water permeability of ultrafiltration components", JIS K3802-1989 "Terminology of membrane and membrane process". Appendix A of this standard is the standard appendix, and Appendix B is the suggested appendix. This standard was proposed by Tianjin Textile Engineering College. This standard is under the jurisdiction of the National Marine Standard and Metrology Center. The drafting unit of this standard is Tianjin Textile Engineering College. The main drafters of this standard are Wei Jianmin, Zhang Huixin, Dai Haiping, and Zhao Changsheng. This standard is entrusted to Tianjin Textile Engineering College for interpretation. 1 Scope
Marine Industry Standard of the People's Republic of China
Test methods for hollow fiber ultrafiltration membranes
Test methods for hollow fiber ultrafiltration membranesHY/T050—1999
This standard specifies the use of spectrophotometry to test the content of polyethylene glycol or protein of different molecular weights in the solution, calculate the retention rate, estimate the pore size of the hollow fiber membrane, and confirm the performance of the hollow fiber ultrafiltration membrane. This standard is applicable to the performance test of hollow fiber ultrafiltration membranes of different materials. 2 Terms
This standard adopts the following definitions.
2.1 Pure water permeability purewaterflux
Under certain flow rate, temperature and pressure, the amount of pure water permeated by the ultrafiltration membrane per unit time and per unit membrane area. 2.2 Rejection
The ratio of specific solutes retained by the membrane to the total specific solutes in the solution. 2.3 Retention molecular weight retentionmolecularweight Under certain conditions, substances of certain molecular weights are retained by the membrane. The minimum molecular weight of the retained substance is the membrane’s retention molecular weight, which is used to characterize the membrane’s separation ability.
3 Test method
3.1 Test of pure water permeability
Under operating pressure of 0.1MPa and room temperature, test the amount of pure water permeated per unit time and per unit membrane area. 3.2 Polyethylene glycol content test
Polyethylene glycol and Dragendoff reagent can generate an orange-red complex, and the polyethylene glycol content in the solution is tested by spectrophotometry. Add polyethylene glycol of known molecular weight to water and pass it through the ultrafiltration membrane. Test the change in the concentration of polyethylene glycol solution before and after ultrafiltration, and calculate the retention rate of the hollow fiber ultrafiltration membrane for polyethylene glycol of this molecular weight. 3.3 Protein content test
Prepare protein solutions of different molecular weights, and use ultraviolet spectrophotometry to test the change in the concentration of the protein solution before and after passing through the ultrafiltration membrane, and calculate the retention rate of the hollow fiber ultrafiltration membrane for proteins of different molecular weights. 4 Main reagents and materials
- Distilled water or water of equivalent purity;
Polyethylene glycol: molecular weight 6000, 10000, 20000 imported packaging; bismuth subnitrate [4BiNO:(OH),BiO(OH)J: analytical grade; glacial acetic acid (CH.COOH): analytical grade;
Potassium iodide (KI): analytical grade;
Approved by the State Oceanic Administration on April 26, 1999
Implemented on July 1, 1999
HY/T 050—1999
Sodium acetate (CHCOONa·3H,O): analytical grade; cytochrome C (molecular weight 13000): freeze-dried preparation; ovalbumin (molecular weight 45000): egg protein tablets; bovine serum albumin (molecular weight 67000): biochemical reagent. 5 Instruments and equipment
Ultraviolet spectrophotometer;
Photoelectric spectrophotometer;
Analytical balance;
A vacuum drying oven;
Dryer;
Volume flask, pipette, beaker, etc.
6 Schematic diagram of ultrafiltration membrane test device
The schematic diagram of ultrafiltration membrane test device is shown in Appendix A (Standard Appendix). 7 Test steps
7.1 Test of pure water transmittance
7.1.1 The test method of pure water transmittance is the same as 3.1. 7.1.2 Calculation of pure water transmittance
Where: F-
Pure water transmittance, m/m2.h);
Pure water transmittance, m;
Membrane area, m2;
-Time used to collect pure water transmittance, h. 7.1.3 Calculation of membrane area
A = naDL
Wherein; A
Membrane area, m;
Number of hollow fiber membranes;
D--diameter of hollow fiber membrane (inner diameter for inner pressure membrane, outer diameter for outer pressure membrane), m; L--effective length of hollow fiber membrane, m.
7.2 Test of polyethylene glycol content
7.2.1 Preparation of reagents
(1)
7.2.1.1 Preparation of Dragendoff reagent
Solution A: Accurately weigh 0.800g of bismuth subnitrate and place it in a 50mL volumetric flask, add 10mL of glacial acetic acid, and then dilute to the mark with distilled water.
Solution B: Accurately weigh 20.000g of potassium iodide and place it in a 50mL brown volumetric flask, and dilute to the mark with distilled water. Dragendoff reagent: Measure 5 mL of solution A and solution B and place them in a 100 mL brown volumetric flask, add 40 mL of glacial acetic acid, and then dilute to the mark with distilled water. Its validity period is half a year. 7.2.1.2 Preparation of acetic acid-sodium acetate buffer Measure 590 mL of 0.2 mol/L sodium acetate solution and 410 mL of 0.2 mol/L glacial acetic acid solution and place them in a 1000 mL volumetric flask to prepare pH 4.8 acetic acid-sodium acetate buffer. 2
7.2.2 Preparation of standard solution
HY/T050—1999
Polyethylene glycol is placed in a vacuum drying oven at 60°C and dried for 4 hours to remove moisture. Accurately weigh 1.000g of polyethylene glycol and dissolve it in a 1000ml volumetric flask. Take 0, 0.5, 1.0, 1.5, 2.0, 2.5, and 3.0mL of polyethylene glycol solution and dilute them in a 100mL volumetric flask to prepare polyethylene glycol standard solutions with concentrations of 0, 5, 10, 15, 20, 25, and 30mg/L. 7.2.3 Preparation of standard curve
Take 5mL of the standard solutions of different concentrations in 7.2.2 and place them in 10mL volumetric flasks. Add 1mL of Dragendoff reagent and 1mL of acetic acid-sodium acetate buffer respectively, and dilute to the scale with distilled water. After standing for 15 minutes, measure the optical density on a photoelectric spectrophotometer at a wavelength of 510nm using 1cm colorimetric blood, with distilled water as the blank. Plot the standard curve with polyethylene glycol concentration as the horizontal axis and optical density as the vertical axis.
7.2.4 Sample testing
7.2.4.1 Preparation of sample solution
Select polyethylene glycol of a certain molecular weight; prepare a polyethylene glycol solution with a concentration of 5000 mg/L, which is used as the solution for evaluating the performance of hollow fiber ultrafiltration membrane.
7.2.4.2 Evaluation of hollow fiber ultrafiltration membrane
The prepared sample solution is run through the hollow fiber ultrafiltration membrane at 0.1 MPa and room temperature, and the ultrafiltrate is collected after 20 minutes. After the stock solution and the ultrafiltrate are diluted by appropriate times, their optical density is determined, and the corresponding concentration is obtained from the standard curve. 7.2.5 Calculation of retention rate
Ru = 2 × 100
Where: Ru——-Retention rate, %
-Polyethylene glycol concentration in the stock solution, mg/L; C2—Polyethylene glycol concentration in the permeate, mg/L. 7.3 Protein content test
7.3.1 Preparation of standard solution
(3)
Bovine serum albumin (BSA) was vacuum dried at 105°C to constant weight. Accurately weigh 1.000 g BSA and dissolve it in a 1000 mL volumetric flask. Pipette 0.2, 0.4, 0.6, 0.8, and 1.0 mL of BSA solution into a 10 mL volumetric flask, add distilled water to dilute to the mark, and prepare concentrations of 20, 40, and 60.80, 100 mg/L bovine serum albumin standard solution. 7.3.2 Preparation of standard curve
Protein has maximum absorption of ultraviolet light at 280nm. The absorption value of protein solution at 280nm is proportional to its concentration. Measure the optical density of the standard solution in 7.3.1 on a UV spectrophotometer at a wavelength of 280nm using 1cm colorimetric blood, and use distilled water as a blank. Plot the protein concentration as the horizontal axis and the optical density as the vertical axis to prepare a standard curve. 7.3.3 Sample testing
7.3.3.1 Preparation of sample solution
Select a protein of a certain molecular weight and prepare a protein solution with a concentration of 1000mg/L to 3000mg/L. The egg white protein solution is used after centrifugation or filtration. The prepared protein solution is used as a solution for evaluating the performance of hollow fiber ultrafiltration membranes. 7.3.3.2 Evaluation of hollow fiber ultrafiltration membrane
The prepared sample solution was run through a hollow fiber ultrafiltration membrane at 0.1MPa and room temperature, and the ultrafiltrate was collected after 20 minutes. The optical density of the stock solution and the ultrafiltrate was measured in the 280nm ultraviolet region, and the corresponding concentration was obtained from the standard curve. 7.3.4 Calculation of interception rate
The calculation formula of the interception rate is the same as formula (3) in 7.2.5. 8 Test resultsWww.bzxZ.net
Two samples were taken from each sample for parallel testing, and the arithmetic mean of their test values ​​was taken as the test result. 3
Test report
The test report shall include the following contents:
HY/T050—1999
a) Type, specification, batch number and manufacturer name of the test membrane sample; b) Room temperature and liquid temperature during the test; c) Test results;
d) Observation of phenomena during the test;
e) Test date;
f) Tester.
The schematic diagram of the ultrafiltration membrane test device is shown in Figure A1.
HY/T050—1999
Appendix A
(Standard Appendix)
Ultrafiltration membrane test device
1—Water tank; 2—Pump 3—Pressure gauge: 4-Ultrafiltration membrane assembly; 5—Valve Ultrafiltration membrane test device and schematic diagram
Appendix B
(Suggestive Appendix)
References
ASTME1343:1990
ASTMD 5090:1990
3JISK3821:1990
JISK3802:1989
Test method for evaluating ultrafiltration membranes by molecular weight cutoff method Standard test data for ultrafiltration permeation rate Test method for pure water permeability of ultrafiltration components Terminology of membranes and membrane processes
HY/T050-1999
Marine
Industry standard of the People's Republic of China
Hollow fiber ultrafiltration Filter membrane test method
HY/T050—1999
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