This standard specifies the determination of iron, magnesium and manganese in food by atomic absorption spectrophotometry. This standard is applicable to the determination of iron, magnesium and manganese in various foods. The detection limit of this method is: 0.2μg/mL for iron, 0.05μg/mL for magnesium, and 0.1μg/mL for manganese. GB/T 5009.90-2003 Determination of iron, magnesium and manganese in food GB/T5009.90-2003 Standard download decompression password: www.bzxz.net

1C567.040
National Standard of the People's Republic of China
GB/T5009.90—2003
G3/T12336—1990
Determininatlon of iron,magnesium and manganese in food
Promulgated on August 11, 2003
Ministry of Health of the People's Republic of China
National Standardization Administration
Implemented on January 1, 2004
GR/T5009.90—2003
This standard replaces B/T1355—Determination of iron, magnesium and manganese in food. Compared with GR1123-1991, the main revisions of this standard are as follows: The Chinese name of the standard was changed to the determination of allergens and chains in foods; GB/T20001.2001 standard revised four parts: Chemical analysis and made revisions to the original structure. This standard was proposed by the Ministry of Health of the People's Republic of China. The drafting of this standard was carried out by: Xie Xinghua, Institute of Nutrition and Food Hygiene, Chinese Academy of Medical Sciences. The original standard was first issued in 1991 and this is the first revision. F.46
1 Scope
Determination of iron, magnesium and manganese in food
This standard specifies the determination of iron, magnesium and manganese in food by photometric method. This standard is applicable to food, and the limits of determination are: 1.3 g/ml, 2.05 g/l, 1.1 k/nL. 2 Principle
GB/T5009.90—2003
After digestion, the sample is introduced into the atomic absorption spectrometer. After initialization, iron, magnesium and lithium absorb the wavelengths of 243.3 nm, 235.2 nm and 279.am respectively. The absorption spectrum is compared with the actual content or ratio of the absorption spectrum and the standard series. 3 Test results
3.1 Hydrochloric acid.
3.2 Sulfuric acid.
3.3 High,
3.4 ​​Mixed acid digestion, 1 aldehyde = 1 high aldehyde. 3.5 3.ril/1. Acid solution: add 32L of electrolytic acid and dilute 1000mL of deionized water. 3.6 Standard
Titanium magnesium, standard filter, standard gold, gold store magnesium, gold (purity greater than 99.9%), each 1.000C, or 1.30 pure gold oxide. Dissolve the acid in three 1600ml volumetric flasks respectively, add 0.5mol/L acid solution and dilute to the scale. Store in ethylene at 4℃, these two solutions are equivalent to 1ms iron, chopsticks, and 3.7 Standard sound efficiency
method, magnesium, chain standard reduction, see Table 1 for the preparation. Table 1 Standard wet preparation
Standard concentration!
Standard concentration range:
Including volume phase (volume flask)
Absent, difficult to use, store in acetone, store, 4 ...
4.2 Atomic absorption spectrophotometer:
GB/T 5009.9--2303
5 Analysis stepswww.bzxz.net
5.1 Test conditions
5.1.1 The preparation of new test specimens shall be especially prevented from various contaminations. All equipment such as electric grinder, internal machine, induction machine, crusher, etc. shall be made of stainless steel. The container used must be fresh or polyethylene products (such as blue pulp, rice, fish, etc.) rinsed with oral technology, and then thoroughly washed with deionized water. Dry samples (such as flour, milk, etc.) are immediately stored in a sealed container after sampling to prevent air and moisture release. 5.1.2 Sample digestion, weigh and collect uniform samples. Wet samples 0.5~1.58 wet samples 2.8~4.08 liquid samples such as beverages 5.3g--10.F tablets! Put in a 250ml. energy beaker, add 2-3mL of digestion acid filter, cover the surface with blood, and heat it on an electric heating plate or medium sand bath. If the digestion membrane is too little, add a few more knives of mixed acid to digest it, and digest it until it is colorless and transparent. Then add a liter of water, heat to remove excess acid, and control the dripping bed in the beaker to nearly 2- -3, cool down, wash with water and transfer to 11mL test tube, add water to the concentration, remove the selected amount of test tube, and make a reagent blank according to the process. 5.2 Determination of the concentration of the standard solution, the method is shown in Table 2, and the preparation parameters are shown in Table 3. Table 2 Preparation method of the standard solution series of different concentrations of the standard solution ... F~4, 3
0. 05-.- 0
Hydroxyprogesterone
0. 5 mcl/L
Weak acid solution
3. ml/:.
New gelatin solution
.5 mol/1.
Hu drop solution
Rare disease
Renal nitrate According to
GB,T5009.90-2303
Other experimental conditions: Instrument, air and flow rate, lamp head, lamp outlet flow should be adjusted to the best state according to the instrument instructions.
5.3 Calculation of results
The concentration series should be determined by the corresponding observation and design of the medical test system. The concentration of the test sample is obtained from the standard curve (see Figure 1), and the formula (1) is used for calculation. x-axxrx10c
XI UU
In this case, the chlorine content of the sample center is mg/100: C
The concentration of the blank sample (obtained from the standard curve) is g/mL (I): The concentration of the blank sample center (obtained from the standard curve) is g/mL (g/mL) The estimated volume should be mL: Multiple: The mass of the sample is g (), and the total volume is shown to two decimal places: 6 The absolute difference between the two independent results obtained under the condition of reciprocity shall not exceed 10% of the arithmetic mean. 54:
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