NY/T 2386-2013 Technical regulations for the prevention and control of rice black-streaked dwarf disease
Some standard content:
ICS65.020
Agricultural Industry Standard of the People's Republic of China
NY/T2386—2013
Technical regulation for rice black-strcaked dwarf disease management2013-09-10Issued
2014-01-01Implementation
Ministry of Agriculture of the People's Republic of China
This standard was drafted in accordance with the rules given in GB/T1.1—2009. This standard was proposed and managed by the Department of Crop Management of the Ministry of Agriculture. NY/T 2386—2013
The drafting units of this standard are: National Agricultural Technology Extension Service Center, Zhejiang Plant Protection and Quarantine Bureau, Jiangsu Dry Goods Protection Station, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Plant Protection Station of Hangzhou City, Zhejiang Province, and Plant Protection and Quarantine Station of Wenling City, Zhejiang Province. The main drafters of this standard are: 1. Hua Di, Guo Rong, Shi De, Ye Jianren, Zhu Yeqin, Yang Rongming, Liu Jin, Zhou Yijun, Wang Daoze, Zhang Hengmu. 1. Scope
Technical regulations for the prevention and control of rice black-streaked dwarf disease
NY/T2386—2013
This standard specifies the control indicators, control strategies and integrated control methods of waterborne black-streaked dwarf disease and its vector, gray leafhopper, in my country. This standard is applicable to the prevention and control of waterborne black-streaked dwarf disease and its vector, gray leafhopper, in various rice-growing areas in China. 2. Normative references
The following documents are indispensable for the application of this document. For all references with a date, only the dated version applies to this document. For all undated references, the latest version (including all amendments) applies to this document. GB42851989 Pesticide Safety Use Standards
GB/T8321.1～8321.9 Pesticide Rational Use Criteria (：)～~(IX) VY/T2156—2012 Technical Regulations for the Prevention and Control of Major Water Frost Diseases 3 Terms and Definitions
The following terms and definitions apply to this document. 3.1
Rice black-streak dwarf disease Rice black-streak dwarf disease A viral disease of rice, transmitted by Laudelphar striatettes Fallen, small brown planthoppcr, SRPH, and the pathogen is Rice black-streak dwarf virus (Rice bluck-streak dwarf virus, RBSDV). 3.2
Rare of viruliferous SBPH The percentage of viruliferous insects of SBPH to the total number of insects surveyed. The determination of the rate of viruliferuus infection was carried out by using the spot immunoassay and the bioassay method, see Appendix A and Appendix B. 3.3
Number of viruliferuus infection The product of the total number of viruliferuus infection per unit area obtained from the SBPH survey and the infection rate. 3.4
Discase management by insect control strategy Control the occurrence of rice black streaked dwarf disease by controlling the viruliferuus infection. 4 Prevention and control strategies
Based on the selection of disease-resistant (tolerant) varieties and the appropriate adjustment of the sowing period, a comprehensive management strategy combining insect control with insect nets or non-woven fabrics and pesticides to control the viruliferuus infection is adopted. 5 Prevention and control indicators
5.1 Prevention and control indicators
The number of viruliferuus infection per 667m2 of hybrid rice seedling fields is 1000, and the number of viruliferuus infection per 667m2 of the field in the early stage is 3000. The number of viruliferuus infection per 667m2 of the field can be appropriately relaxed for other varieties and types of fields.
5.2 Survey methods
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See Appendix C
5.3 Fields for control
The fields with the number of SBPHs reaching the control target within 20 days after transplanting the seedlings from the 2-5-month period are determined as control targets. See Appendix D for the calculation of the number of SBPHs carrying the virus. 6 Key points of control technology
6.1 Agricultural control
6.1.1 Planting disease-resistant (tolerant) varieties
According to local conditions, promote and apply high-quality, high-yield, disease-resistant (tolerant) varieties. 6.1.2 Adjust rice sowing period
According to the migration period of SBPHs, appropriately reduce the sowing period of water so that the sensitive period of rice disease avoids the peak period of SBPHs migration. 6.1.3 Centralized seedling raising
The site selection should be far away from wheat fields and wasteland, and the seedlings should be raised in a concentrated area. Strengthen the management of intercropping fertilizer and water, apply nitrogen fertilizer, phosphorus fertilizer and potassium fertilizer scientifically and rationally, promote the healthy growth of rice and improve the plant's resistance to stress and disease. 6.1.4 Clean the fields
Combined with methods such as farmland plowing, intertillage weeding and chemical weeding, do a good job in weed control in insect source areas of seasonal and winter idle fields to reduce the number of overwintering insect sources.
6.2 Physical control
The seedlings should be covered with insect-proof nets or non-woven fabrics throughout the process, that is, before the rice seedlings fall or after the film is removed, use an insect-proof net with a mesh size of more than 20 or a non-woven fabric with a specification of 15g/m2 to 20g/m2 to cover the seedlings. When covering with insect-proof net, supports should be set up all around, and the top of the supports should be kept at a height of more than 30 cm from the seedlings to facilitate ventilation and light transmission. 6.3 Chemical control
6.3.1 Control in wheat fields and fallow land
In seriously diseased areas, combine the control of wheat mildew, aphids and other diseases. Also control ash fly ash. For row 667m, 30g of 25% pymetrozine wettable powder or 150mL~~200ml of 20% isocyanate emulsifiable concentrate can be selected and sprayed with 30L~45L of water. 6.3.2 Seed dressing with pesticides
Choose thiamethoxam, imidacloprid and other pesticides for seed dressing. For every dry gram of rice seeds, use 1.2g~3.5g of 30% nitrobenzene seed treatment suspension or 2ml~ml of 60% imidacloprid seed treatment suspension. 6.3.3 Rice seedling field control
For every 667m2, use 25mL of 20% nitrobenzene aqua, or 30g of 25% pyriproxyfen moist-release powder, or 150ml~200ml of 20% isoprocarb emulsifiable concentrate, or 60l~80nl of 10% ethoxyfen suspension, add 30L~45L of water, and spray evenly on the leaves. After the first application of pesticides, conduct a daytime control effect survey. Depending on the disease situation, control 2~3 times continuously with a control interval of 7d~1d. Control once 2d~3d before transplanting rice seedlings or when covering the seedlings and removing the net (cloth), and transplant them. 6.3.4 Field control
Mountain seedlings should be controlled once within 20d after transplanting. For every 667In2, 20% nitenpyram aqueous solution 25mL, or 10% ethoxyfen suspension 60ml.~-80mL, or 25% pymetrozine wettable powder 30g, or 20% isoprocarb emulsifiable concentrate 150mL~200ml., or 25% thiamethoxam water dispersible granules 4g~6g can be used, add 30L~-45L of water, and spray evenly on the surface. After application, keep a 3cm~5cm water layer for 3d~5d to ensure the control effect.
7 Remedial measures
In the early stage of the disease, use 50ml of 8% Ningnanmycin aqueous solution or 60g of 30% chlorpyrifos wettable powder per 667m2, and spray the leaves evenly once or twice with 30L-45L of water to relieve the symptoms. If the disease is serious, remove the diseased plants in time, replant 2
seedlings, or peel and cut from healthy bushes.
8 Evaluation of control effect
8.1 Control record
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Establish a record of anti-silting, and keep a record of the type, dosage and time of the drug used for each control. The record should be kept for no less than 2 years. 8.2 Evaluation of control effect
Check the control effect of the vector gray leafhopper 3cl~5 after application of the drug, and the number of gray leafhoppers is checked in the same way as in 5.2. For fields where the number of insects still exceeds the control index after prevention, supplementary treatment is required. After the disease is stable at the end of rice jointing, a field survey is carried out. The fields are divided into different types according to the cropping type, variety, and growth period. Three fields are surveyed for each type. Linear parallel sampling is carried out, and 200 clusters are surveyed for each field. The total number of clusters and plants investigated, the number of diseased clusters and the number of diseased plants are recorded, and the rice disease rate (plant) is calculated (see Appendix E). The degree of disease and damage losses (see Appendix F) and the control effect are evaluated.
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(Normative Appendix)
Rapid determination method of spot immunity for the rate of infection of gray leafhoppers If the gray leafhoppers adults or old ones are collected in the future, place the single head insect in a 200u centrifuge tube and add 100uL carbonate buffer solution. Use a wooden toothpick to crush it and make the sample to be tested. Draw 0.5cm x 0.5cm squares on the nitrocellulose membrane and add 3μl of the sample to each square. Let it dry at room temperature. Block the membrane with 4% bovine serum (or 0.4% BSA) at 37°C.After 5 hours, immerse in enzyme-labeled monoclonal antibody (blocking solution diluted 500 times) for incubation for 1.5 hours, wash and immerse in solid color substrate solution for 0.5 hours. Wash three times in PBST for 3 minutes each time. Check the reaction type (positive reaction of infected gray planthoppers) and record the number of infected insects. Appendix B
(Normative Appendix)
Biological determination method of the virus rate of Laodelphax striatelluswwW.bzxz.Net
NY/T2386—2013
Under the condition of insect-proof net (net cover), sow the main local rice varieties. When the seedlings are in the 2-leaf stage to 3-leaf stage, each seedling is covered with a copper yarn tube or glass tube with two connected ends. Put an adult of Laodelphax striatellus in each tube with a straw, cover the hole at one end with gauze, feed a single tube, a single segment, and a single insect for 4 days, and then remove the adult (check the feeding once 2 days after the insect is inoculated. If the insect survives less than 2 days, it should be removed together with the seedlings and not counted). Move the single plant of Laodelphax striatellus to the insect-proof shed for further observation. If there are Laodelphax striatellus egg masses and low-age nymphs appear, spray insecticide in time. After the disease is stable, record the number of plants infected with rice black-streaked dwarf disease and calculate the plant rate, which is the Laodelphax striatellus virus rate of this generation. 5
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Appendix C
(Normative Appendix)
Survey Method for the Insect Traps of Gray Planthopper
Divide the fields into three types according to the variety and growth period. Each type of field has 3 plots. Each plot of the fast field is checked with 10 points. The white porcelain pot is used to sweep the chessboard sampling, and each point is 0.15m; each fast field is checked with 50 clusters. Parallel jumping sampling is used. Each point is sampled with 2 clusters. The white porcelain pot is used to shoot food and record the number of gray planthoppers. Determine the gray planthopper infection rate and calculate the number of gray planthoppers. Appendix D
[Normative Appendix]
Calculation Method for the Insect Traps of Gray Planthoppers and Insect Traps D.1 The infection rate of leaping flyhoppers is calculated according to formula (D.1). ,
Where:
L. —The virus-carrying rate of gray leafhopper, in percentage (%); N.——The number of gray leafhoppers infected with the virus;
N,——The total number of gray leafhoppers tested.
2The number of gray leafhoppers infected with the virus is calculated according to formula (D.2). D.2
N, = N, XL.
Where:
N. -The number of gray leafhoppers infected with the virus per unit area: NThe total number of gray leafhoppers per unit area
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E.1The incidence rate of rice from (plants) is calculated according to formula (E.3). In the formula:
Appendix E
(Normative Appendix)
Incidence rate and control effect
Incidence rate of clusters (plants), unit is white percentage (%); number of diseased clusters (plants);
Total number of clusters (plants) investigated.
E.2 The control effect of the disease is calculated according to formula (E.2). p
In the formula:
-control effect, unit is percentage (%); 1
I-the incidence rate of clusters (plants) in the blank control area after control, unit is white percentage (%) I-the incidence rate of clusters (plants) in the treated area after control, unit is percentage (%). E.3 The reduction rate of planthopper population is calculated according to formula (E3). DN
N×100
Wherein:
Export reduction rate, in percentage (in): insect quantity before control;
-insect quantity after control.
E.4 The control effect of gray leafhopper is calculated according to formula (F.4). P. =
100-D
Wherein:
P--the control effect of gray leafhopper, in percentage (in); I,——the insect population reduction rate in the control area, in percentage (); D
X 100-
Blank control area export reduction rate, unit is percentage (%), (E.1)
Appendix F
(Normative Appendix)
Grading standard for the incidence of rice black-streaked dwarf diseaseGrading standard for the incidence of rice black-streaked dwarf disease is shown in Table F1.Table F.1
Incidence rate of plant, %
Ratio of affected area%
Light occurrence
( Grade 1）
Rice black-streaked dwarf disease severity grading standard Moderate to mild outbreak
(Grade 2)
Moderate occurrence
(Grade 3)
[10,20
Moderate to severe outbreak
(Grade 4)
2>20,30
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Severe outbreak
(Grade)30
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Reoccurrence
(level)30
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Reoccurrence
(level)
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