NY/T 2423-2013 Guidelines for testing specificity, consistency and stability of new plant varieties Adzuki beans NY/T2423-2013 |tt||Standard compressed package decompression password: www.bzxz.net

ICS 65.020.20
Agricultural Industry Standard of the People's Republic of China
NY/T2423—2013
Guidelines for the conduct of tests for distinctness, uniformity and stability-Adzuki bean
[Vigna angularis(Willd)Ohwi & Ohashi ]Issued on September 10, 2013
Implemented on January 1, 2014
Ministry of Agriculture of the People's Republic of China
3Issued
Normative referenced documents
Terms and definitions
Requirements for propagation materials
Test methods
Determination of specificity, consistency and stability resultsCharacter table
Grouped traits
10Technical questionnaire:
Appendix A (Normative Appendix)Character table of adzuki beanAppendix B (Normative Appendix)
Appendix C (Normative Appendix)
Interpretation of the character table of adzuki bean
Format of technical questionnaire on adzuki bean
NY/T 2423--2013
AY/T2423—2013
This standard was drafted in accordance with the rules given in GB/T1.12009. This standard is proposed by the Department of Science, Technology and Education of the Ministry of Agriculture. This standard is approved by the National Technical Committee for Standardization of New Plant Varieties Testing (SAC/TC277). The drafting units of this standard are: Institute of Crop Sciences, Chinese Academy of Agricultural Sciences. Science and Technology Development Center of the Ministry of Agriculture. The main drafters of this standard are Cheng Xuzhen, Liu Ping, Xia Lixia, Xia Suhua, Liu Changyou, Xu Ning, II
1 Scope
NY/T2423—2013
Guidelines for testing specificity, consistency and stability of new plant varieties
This standard specifies the technical requirements and general principles for the specificity, consistency and stability testing of new varieties of adzuki beans [Vignuungutaris (Willd) Ohwi & Ohashi]. This standard is applicable to the testing and result determination of specificity, consistency and stability of new varieties of adzuki beans. 2 Normative references
The following documents are indispensable for the application of this document. For all referenced documents with a date, the version with the date of the date applies to this document. For any undated referenced document, the latest version (including all amendments) shall apply to this document. GB2905 Method for determination of crude protein in seeds of cereals and legumes (semi-micro Kjeldahl method) GB/T3543 Procedure for inspection of crop seeds
GB5006 Method for determination of crude starch in cereal kernels (improved hydrochloric acid hydrolysis-polarimetry) GB 5511 Method for determination of crude protein in inspection of grains and oilseeds GB/T5514 Method for determination of starch in inspection of grains and oilseeds GB5519 Method for determination of thousand-grain weight in inspection of grains and oils GB 10161 Method for determination of crude starch in grain kernels GB/T19557.1 Guidelines for specificity, consistency and stability testing of new plant varieties General 3 Terms and definitions
Terms and definitions defined in GB/T19557.1 and the following terms and definitions shall apply to this document. 3.1
Single measurement of a group of plants or parts of plants Single measurement of a group of plants or an organ or part of a plant to obtain a group record. 3.2
f measurement of a number of individual plants or parts uf plants Individual measurement
Measurement of a group of plants or an organ or part of a plant one by one to obtain a set of individual records. 3.3
Visual assessment by a single observation of a group of plants or parts nf plants Monthly measurement of a group of plants or an organ or part of a plant to obtain a group record. 3.4
Visual assessment by olxservation of individual plants or parts uf plants Visual assessment by olxservation of individual plants or parts uf plants 4 Symbols
The following symbols apply to this document:
MG: group measurement.
MS: individual measurement.
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VG: Group self-test,
VS: Individual self-test
QI. Quality traits,
QN, Quantitative traits
PQ: Pseudo-quality traits.
(a): The marked content is explained in detail in B.2 of Appendix 3. (+); The marked content is explained in detail in B.3 of Appendix B. II: The underlined content in this document is a special reminder of the scope of application of the test trait. 5 Requirements for propagation materials
5.1 Propagation materials are provided in the form of seeds.
5.2 The number of seeds submitted is at least 1kg. 5.3 The submitted propagation materials should be healthy in appearance, high in vitality, and free of pests and diseases. The specific quality requirements of propagation materials are as follows: purity ≥ 98.0%, germination rate 285%. Water content ≤ 12%. 5.4 The propagation materials submitted generally do not undergo any treatment that affects the normal expression of variety traits (such as seed coating treatment): if they have been treated, detailed descriptions of the treatment should be provided.
5.5 The propagation materials submitted should comply with the relevant regulations of China's plant quarantine. 6 Test method
6.1 Test cycle
The test cycle is at least 2 independent growth cycles. 6.2 Test location
The test is usually carried out at one location. If some traits cannot be fully expressed at this location, they can be observed at other qualified locations.
6.3 Field test
6.3.1 Test design
Please plant varieties and similar varieties adjacent to each other. At least 150 plants are planted in each plot with a row spacing of 50cm and a plant spacing of 15cm-~20cm. Each test variety is repeated twice. 6.3.2 Field management
The field management plan shall be the same as the field management measures at the test site. The field management of the application variety, similar varieties and standard varieties shall be strictly consistent.
6.4 Character observation
6.4.1 Observation period
Character observation shall be carried out according to the growth stages listed in Table A.1 and Table A.2. The growth stage description is shown in Table A.1. 6.4.2 Observation method
Character observation shall be carried out according to the observation methods specified in Appendix A and Appendix IB (VG, VS, MG, MS): For some character observation methods, see B.2 and B.3.
6.4.3 Number of observations
Unless otherwise specified, the number of samples for individual observed traits (VS.MS) shall not be less than 20. When observing the organs or parts of the plants, the number of samples for each plant shall be 1. The observed traits (VG, MG) of the population should be observed in the whole plot or a mixed sample of a specified size. 6.5 Additional tests
When necessary, the traits in Table A, 2 or the traits listed in this document can be used for additional tests. 7 Determination of specificity, consistency and stability results 7.1 General principles
The determination of specificity, consistency and stability shall be carried out in accordance with the principles determined in GB/T19557.1. 7.2 Determination of specificity
NY/T 2423—2013
The applied variety should be clearly distinguished from all known varieties. In the test, when the applied variety has obvious and reproducible differences with similar varieties and varieties in at least one trait, it can be determined that the applied variety has specificity. 7.3 Determination of consistency
When determining the consistency of the test variety, a 1% population standard and a probability of acceptance of at least 95% are used. When the sample size is 120 plants, a maximum of 3 heteromorphs can be allowed.
7.4 Determination of stability
If a variety has consistency, it can be considered stable. Generally, qualitative testing is not performed. If necessary, the next generation of seeds of the variety can be planted. If there is no obvious change in the expression of traits compared with the previously provided propagation materials, the variety can be determined to be stable.
8 Trait table
According to the test needs, the traits are divided into basic traits and selected traits. Basic traits are traits that must be used in the test. See Table A.1 for the basic traits of adzuki beans and Table A.2 for the selected traits.
8.1 Overview
The trait table lists the trait name, expression type, expression state and corresponding code and standard variety, observation period and method, etc. 8.2 Expression type
According to the expression method of the trait, the traits are divided into three types: quality traits, pseudo-quality traits and quantitative traits. 8.3 Expression state and corresponding code
8.3.1 Each trait is divided into a series of expression states to facilitate the definition of traits and standard descriptions. Each expression state is assigned a corresponding digital code to facilitate data recording, processing and the establishment and communication of variety descriptions. 8.3.2 For quality traits and pseudo-quality traits, all expression states should be listed in the test guide: For quantitative traits, in order to shorten the length of the trait table, the expression states with even-numbered codes can be omitted, and the expression states with even-numbered codes can be summarized in the form of the previous expression state to the next expression state.
8.4 Standard varieties
The table of traits lists standard varieties for reference of expression status of some traits, to help determine the different expression status of the traits and correct the differences caused by environmental factors.
9 Grouped traits
In this document, the grouped traits of varieties are as follows: a) Young stems: epicotyl anthocyanin II color (Table A.1 Neutral trait 1). b) Plants: Growth habit (Table A.1 Neutral trait 9). c) First flowering time (Table A.1 Neutral trait 3). d) Seed: Shape (Table A. 1 Neutral trait 17). e) Seed: Color (Table A.1 Neutral trait 18). Grains: Tremor color (Table A. 1 Neutral trait 21). 3
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10 Technical Questionnaire
The applicant shall fill in the technical questionnaire of adzuki beans in the format given in the attached document. A.1 Basic characteristics of adzuki beans
See Table A.1.
Stem: adipose axis anthocyanin Wu Sheng color
Leaf; single piece shape
Beginning of flowering
Compound leaf: size of terminal leaflet
Compound leaf: shape of terminal leaflet
(ai(+)
Compound leaf: degree of green
Flower: flower cyanin Wu Sheng color
Flower: degree of yellow
Plant, growth habit|| tt||Plant: Height
Maturity
Appendix A
[Normative Appendix]
Table of adzuki beans
Table A.1 Decline and decline of basic traits of adzuki beans
Observation period
and method
Expression status
Narrow oval
Broad oval
Narrow oval
Broad oval
Semi-climbing
Standard varieties
NY/T2423—2013
Xingtang red adzuki beans (HG107)
Yellow adzuki beans (B0572)||tt| |Jihong No. 4 (B3992)
Millet beans (R1571)
Aohong No. 3 (H4804)
Jihong No. 4 (B3992)
Zhonghong 11 (B1705)
Split-leaf beans (Ks193)
Jihong No. 4 (13932)
Millet beans (B1371)www.bzxz.net
Zhong 961 (B4806)
Jihong No. 4 (B3992)
Maxiaodou (B1480)
Jihong No. 4 (R3992)
Zaoxiongdou (B1546)|| tt||Jihong No. 4 (H3992)
Bao M908-15 (B4708)
Early Black Bean (B1546)
Jihong No. 4 (B992)
Xixian Adzuki Bean (B0611)
Xingtang Adzuki Bean (B9107)
Zhong 96806)
Jihong No. 4 (3992)
Yellow Adzuki Bean (BG572)
Black Adzuki Bean (L29SC)
Xingpian Adzuki Bean (I30107)
Zhong 961 (M806)
Jihong No. 4 (T399 2)
Red adzuki beans (B0137)
Rice adzuki beans (B1571)
Ma adzuki beans (B1480)
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Plant: Three tea nodes
Plant: Single plant division
Plant: Fruiting
Hangzhou plant; Number of fruits per plant
Fruit: Flaccidity
Fruit shape
Fruit: Color
Seed: Number of single grains
Zi: Length
Seed: Fried color
Seed: Red degree is only applicable to red varieties QN||tt| |Grain: shape
Grain: 100-grain weight＊
Table A.1 (continued)
Measurement period
Concept of our concept
Straight and simple shape
Knife-shaped
Short cylindrical
Long cylindrical
Standard and elegant varieties
Zhong 968
Yucheng red bean (2789)
Su 4: No. 4 (H3992)
Zhongyin 96 (H1806)
Yellow bean (E0372)
Xingdian red bean (30107)
200013 (148C6)| |tt||Binhong No. 4 ((13992)
Zhong (31806)
Jihong No. 1 (B3992)
Xingtang Red Bean (13:107)
B3992 (Jixiang No. 1.4)
Yucheng Red Bean (B2789)
Jihong No. 4 (K3992)
Black Bean (F2930)
Kehong No. 4 (B992)
Xingtang Red Bean (B107)
Yucheng Red Bean (B2789)
200013 (1806)
Yucheng Red bean (B2789)
130572 (Huang Xiaoyuan)
B278 (Yucheng red bean)
B3992 (Yihong 4)
BD107 (Xingtang red bean)
White bean (R22G1)
Gexian bean (EC61t)
Yellow bean (E0572)
Jihong 4 (I3992)
Period (B0257)
Black bean (B2930)
Flower bean (B2132)
Ma Xiao (B1180)
Medium size: 967(14805)
Jihong 4 (BR992)
Nanzheng County Adzuki Bean (B429C)
Tanghong Adzuki Bean (0107)
Zhong 1951(14806)
H1A80 (Ma Adzuki Bean)
B1571 (Rice Small Bean)
B0572 (Yellow Small Bean)
Jihong 4→(B3992)
Nanzheng County Adzuki Bean (B4290)
Seed ：Seed coat glossiness
Selected traits of adzuki bean
See Table A.2.
Plant: Mosaic virus disease
Plant: Leaf spot
Plant: Aphid
Seed: Resistance to bean weevils
Seed: Protein content
Seed: Starch content
Seed: Sand production rate
Table A1 (continued)
Observation period
and method
Expression status
Table A, 2 Table of selected traits of adzuki bean
Observation period
and method
Expression status
Standard variety
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Xingtang red adzuki bean (H0107)
Gonghong No. 4 (B3992)
Standard variety
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B.1 Growth stage of adzuki bean
See Table B.1.
Growth stage code
B.2 Explanation of multiple traits
Appendix B
(Normative Appendix)
Explanation of adzuki bean trait table
Table B.1 Adzuki bean growth stage table
+planted
Liu Sheng single leaf exposed mountain flat surface
The first flower appears on the plant
About 50% of the plants have flowers
About 71% of the plants have flowers
About 7% of the fruits show mature color
About 70% of the leaves turn green or fall off
Compound leaf: The top of the compound leaf matures in the upper 1/3 of the middle of the upper part (a)
B.3 Explanation of a single trait
Trait 2 Leaf: Single leaf shape, find Figure B.1. Weilang round
Character 3 Flowering period
Broad oval
Figure B.1 Leaf: Single leaf shape
Count the number of days from sowing to the first flower opening in the group. According to the number of first flowers, it is divided into 3 levels. (First flower day 5d)
Medium (50d first flower day 60d)
Late (first flower number 60d)
Character Compound leaf: The shape of the terminal leaflet is shown in Figure B.2. 0
Narrow oval
Broad oval
Figure B.2 Leaf: The shape of the terminal leaflet of the compound leaf
Character 9 Plant: Growth habit, see Figure B.3. Erect
Semi-climbing
Figure B.3 Plant: Growth habit
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Character 10 Plant: Height, see Figure B.4. Take the selected sample plants as the observation object and measure the height of each main stem from the cotyledon node or the ground to the place where the petiole of the last compound leaf is attached. According to the height of mature plants, they are divided into 5 levels: Very short (plant height <45cm)
Short (45.cm plant height 55cm)
Medium (55.cm≤plant height 65.cm)
High (65cm≤plant height 75cm)
Very tall (plant height ≥75cm)
The last compound leaf is located at
Cotyledon node
Figure B.4 Plant: Height
Character 11 Maturity period, the number of days from sowing until about 70% of the fruits are mature, the beans show mature color, and become hard. According to the number of days to maturity, they are divided into E levels.
Very early (maturity period 80d)
Early (80d maturity period <90d)
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