This standard specifies the detection method and reagents for monkeypox virus (SPV). This standard is applicable to the detection of monkey SPV. GB/T 14926.64-2001 Detection method of monkeypox virus in experimental animals GB/T14926.64-2001 Standard download decompression password: www.bzxz.net

ICS65.020.30
National Standard of the People's Republic of China
GB/T14926.64—2001
Laboratory animal
Microbiological examination methods (4)
Laboratory animalMicrobiological examination methodsPromulgated on August 29, 2001
General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China
Implementation on May 1, 2002
GB/T14926-64—2001
This standard specifies the detection methods for acquired viruses. Monkeypox virus is a human pathogen. It is a natural host of monkeypox virus. Monkeypox virus is a must-test item for SPF and SPF. Therefore, the detection method of this virus is added. The Ministry of Science and Technology of the People's Republic of China has issued this standard. The drafting unit of this standard is: China Laboratory Animal Society. The main drafter of this standard is: Tian Kegong.
1 Scope
National Standard of the People's Republic of China
Laboratory animal-Method for examination of Simian Poxwirus (SPW)
This standard specifies the detection method of Simian Poxwirus
This standard applies to SI
2 Referenced Standards
The following standards include the following articles, which have become the standards of this standard by citing them in this standard GB/T14926.64-2001
. When this standard was published, the versions shown were valid. All standards will be revised and revised + use this standard GB/T14926CA
through the use
|001 Laboratory Animal Quality
GB/T14926
3 Principle
Vaccinia virus and mono
Laboratory Animal Immunization
There is a close antigenic relationship
4 Main Reagents and Equipment
4.1 Reagents
4.1.1 ELISA Antigens
4.1.1.1 Specific Antigens, the right
The possibility of calling the latest version of the standard. Vaccine virus antigen detection SPV antibody in serum
The whole cabinet is in use, and the product certificate is 15 girls. After 2-3 days of vaccination, when the lesions reach ++~100, harvest. After freeze-thaw three times, the supernatant was subjected to ultracentrifugation and then prepared into ELISA antigen.
4.1.1.2 Normal antigen: BHK21 cells were thawed and broken, and the supernatant was obtained by low-speed centrifugation to remove the cells. 4.1.2 Original slide
BHK21 cells were inoculated with vaccinia virus. 12 days after inoculation, when the lesions reached +, they were dispersed with trypsin in a biosafety cabinet, washed with PBS, and sliced. While drying at room temperature, they were irradiated at 20 cm under ultraviolet light for 30 minutes, fixed with cold acetone for 10 minutes, and stored at -20℃. 4.1.3 Positive serum
Vaccinia virus antigen-immunized cystic erythrocyte serum
4.7.4 Negative serum
Serum obtained without SPV or vaccinia virus infection. 41.5 Required binding substances
Approved by the General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China on August 29, 2001 and implemented on May 1, 2002
GB/T14926-64-2001
Horseradish peroxidase-labeled sheep or rabbit anti-Lin IgG antibody: or horseradish peroxidase-labeled Staphylococcus aureus protein A (SPA), 42 ApparatusWww.bzxZ.net
4.2.1 Enzyme marker only.
4.2.2 Ordinary microscope.
4.2.3 37C culture medium or water tank
Detection method
51 ELISA method (see GB/T14926.50-2001) shall be used for direct inspection. Serological test is carried out by IEA method (see GB/14926.51-2001). 5.2
Fin fruit determination
For positive test results, the same method or another method is used for retry. If it is still positive, it is determined to be positive. Serological report
The results of the determination are reported
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