This part specifies the methods and basic requirements for field efficacy plot tests of fungicides for the control of soft rot of Chinese cabbage (Erwinia carotovora). This part applies to field efficacy plot tests and efficacy evaluation of fungicides for the control of soft rot of Chinese cabbage for registration. Other field efficacy tests shall refer to this part. GB/T 17980.114-2004 Guidelines for field efficacy tests of pesticides (II) Part 114: Efficacy test of fungicides for the control of soft rot of Chinese cabbage GB/T17980.114-2004 Standard download decompression password: www.bzxz.net
This part specifies the methods and basic requirements for field efficacy plot tests of fungicides for the control of soft rot of Chinese cabbage (Erwinia carotovora). This part applies to field efficacy plot tests and efficacy evaluation of fungicides for the control of soft rot of Chinese cabbage for registration. Other field efficacy tests shall refer to this part.

GB/T17980.114—2004
Field efficacy test is one of the important contents of pesticide registration management and an important technical basis for the formulation of pesticide product labels. Labels are the only guide for the safe and rational use of pesticides. In order to standardize the methods and contents of pesticide field tests, make the tests more scientific and unified, and align with international standards, so that my country's efficacy test reports have international recognition, the national standards for my country's field efficacy test guidelines are specially formulated. This series of standards refers to the field efficacy test guidelines of the European and Mediterranean Plant Protection Organization (EPPO) and similar guidelines of the Food and Agriculture Organization of the United Nations (FAO) in the Asia-Pacific region. It is formulated based on my country's actual situation and verified by a large number of field efficacy tests. Cabbage soft rot is one of the main diseases of Chinese cabbage in my country, and fungicides are often used for prevention and control in production. This part of GB/T17980 is specially formulated to determine the optimal dosage of pesticides for controlling Chinese cabbage soft rot, test the effects of pesticides on crops and non-target beneficial organisms, and provide a basis for the efficacy evaluation and safe and reasonable use technology of fungicide registration. This part is one of the standards in the series of guidelines for field efficacy tests of pesticides (II), but it is an independent part in itself. This part was proposed by the Ministry of Agriculture of the People's Republic of China. The drafting unit of this part: Pesticide Control Institute of the Ministry of Agriculture. The main drafters of this part: Liu Naizhi, Xie Bing, Wu Xinping, Li Jianqiang, Li Baoju, Chen Bo, Wang Wanli. This part is interpreted by the Pesticide Control Institute of the Ministry of Agriculture. 1 Scope
Guidelines for field efficacy tests (II)
GB/T 17980.114—2004
Part 114: Fungicides for the control of soft rot of Chinese cabbage This part specifies the methods and basic requirements for field efficacy plot tests of fungicides for the control of soft rot of Chinese cabbage (Erwinia carotovora).
This part is applicable to field efficacy plot tests and efficacy evaluation of fungicides for the control of soft rot of Chinese cabbage for registration. Other field efficacy tests shall refer to this part.
2 Test conditions
2.1 Selection of test objects, crops and varieties The test object is soft rot.
The test crop is Chinese cabbage, and local conventional varieties are selected, and the variety name is recorded. 2.2 Environmental conditions
Field trials should be conducted in open fields or greenhouses where the disease has been severe in previous years. The cultivation conditions (variety, soil type, fertilization, planting period, etc.) of all test plots should be consistent and in line with local scientific agricultural practices (GAP). If irrigation is used, record the irrigation method, time and amount of water.
If fumigants and fogging agents are tested in greenhouses, each treatment should use a single greenhouse or an isolated greenhouse. 3 Experimental design and arrangement
3.1 Agents
3.1.1 Test agents
Indicate the trade name or code, generic name, Chinese name, dosage form content and manufacturer of the agent. The test agent treatment shall be no less than three doses or the dosage specified in the agreement (the test agreement signed by the test entrusting party and the test undertaking party). 3. 1.2 Control agents
Control agents should be registered products that have been proven to have good efficacy in practice. The type and mode of action of the control agent should be similar to that of the test agent and the local commonly used dosage should be used. Special circumstances may depend on the purpose of the test. 3.2 Plot arrangement
3.2.1 Plot arrangement
The plot treatment of the test agent, control agent and blank control is arranged randomly, and special circumstances should be explained. 3.2.2 Plot area and repetition
Plot area: 15m2~50m2, and the greenhouse is not less than 8m2. Number of repetitions: at least 4 repetitions.
3.3 Application method
3.3.1 Usage method
Carry out according to the requirements of the agreement and the instructions on the label. The application of pesticides should be adapted to local scientific agricultural practices. 3.3.2 Types of equipment used
Use commonly used equipment in production and record all information on the type of equipment used and operating conditions (such as working pressure, nozzle diameter). Application 1
GB/T17980.114—2004
The dosage should be accurate and evenly distributed. Any deviation of the dosage exceeding ±10% should be recorded. 3.3.3 Application time and frequency
Follow the agreement requirements and label instructions, record the number of applications and the date of each application and the crop growth period. Usually the first application is when the disease first occurs, and the subsequent application depends on the development of the disease and the duration of the drug. 3.3.4 Dosage and volume
Use according to the agreement requirements and the dosage indicated on the label. Usually the active ingredient content of the drug is expressed as g/hm2. At the same time, record the multiple of application and the amount of liquid per hectare (L/hm2). 3.3.5 Data requirements for pesticides for controlling other diseases and insect pests If other pesticides are used, pesticides that have no effect on the test pesticides and test objects should be selected, and all test plots should be treated uniformly. They should be used separately from the test pesticides and control pesticides to minimize the interference of these pesticides and record accurate data on the application of such pesticides.
4 Investigation, recording and measurement methods
4.1 Meteorological and soil data
4.1.1 Meteorological data
During the test, rainfall (rainfall type and daily rainfall, expressed in mm) and temperature (daily average temperature, maximum and minimum temperature, expressed in °C) data should be obtained from the test site or the nearest meteorological station. Adverse climatic factors that affect the test results during the entire test period, such as severe and long-term droughts, heavy rains, etc., should be recorded. 4.1.2 Soil data
Record data such as soil type, soil fertility, moisture (such as dry, wet or waterlogged), soil cover (such as crop residues, plastic film covering, weeds), etc.
4.2 Survey methods, time and frequency
4.2.1 Survey methods
Survey all vegetable plants in each plot, record the total number of surveyed plants and the number of diseased plants. 4.2.2 Survey time and frequency
Perform according to the requirements of the agreement. Usually, the disease base is investigated before the application of medicine, and the control effect is investigated 7 to 14 days before the next application of medicine and after the last application of medicine. 4.2.3 Calculation method of drug efficacy
Disease rate (%) = total number of surveyed plantswww.bzxz.net
Number of diseased plants
F×100
Blank control area disease rate - treatment area disease rate × 100 Control effect (%) =
4.3 Other effects on crops
Blank control area disease rate
Observe whether the crops have been damaged by medicine. If so, record the degree of damage. In addition, other beneficial effects on crops (such as promoting maturity, stimulating growth, etc.) should also be recorded.
Record pesticide damage using the following methods:
a) If the pesticide damage can be measured or calculated, it should be expressed in absolute values, such as plant height, plant weight, fruit shape and fruit setting rate. b) In other cases, the extent and frequency of pesticide damage can be estimated using the following two methods: 1) Record the pesticide damage situation in each plot according to the pesticide damage grading method, expressed as one, ten, ten ten, ten ten ten, ten ten ten ten. Grading method of pesticide damage:
1. No pesticide damage;
10: Mild pesticide damage, which does not affect the normal growth of crops; 10-10: Obvious pesticide damage, which can be restored and will not cause crop yield reduction; 10-10-10: Severe pesticide damage, which affects the normal growth of crops and causes certain losses to crop yield and quality. Generally, compensation for part of the economic losses is required;
GB/T17980.114—2004
10-10-10: Severe pesticide damage, crop growth is hindered, and yield and quality losses are serious. Economic losses must be compensated. 2) Evaluate the percentage of pesticide damage in each test plot compared with the blank control. At the same time, the symptoms of crop pesticide damage (dwarfing, chlorosis, deformity) should be accurately described, and physical photos, videos, etc. should be provided. 4.4 Impact on other organisms
4.4.1 Impact on other pests and diseases
Any impact on other pests and diseases should be recorded, including beneficial and harmful impacts. 4.4.2 Impact on other non-target organisms
Record the impact of the pesticide on wild organisms and beneficial insects in the test area. 4.5 Product yield and quality
Record the yield of each plot, expressed in kg/hm2. 5 Results
The results obtained from the test shall be analyzed by biostatistical methods (using DMRT method), and a conclusion report shall be written in a formal format, and the test results shall be analyzed. The original data shall be kept for inspection and verification.
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