Some standard content:
National Standard of the People's Republic of China
Rules for agricultural seed testing-General directives
1 Subject content and scope of application
GB/T 3543. 1—1995
Replaces GB3543--83
This standard specifies the seed sample support procedure, the operating procedures of seed quality testing items, the basic requirements for testing and the result report. This standard applies to the testing of crop seed quality. 2 Reference standards
GB/T3543.2 Inspection procedures for crop seeds Sampling GB/T3543.3 Inspection procedures for crop seeds Purity analysis GB/T3543.4 Inspection procedures for crop seeds Germination test
GB/T3543.5 Inspection procedures for crop seeds Authenticity and variety purity identification
GB/T3543.6 Inspection procedures for crop seeds Determination of moisture content
GB/T3543.7 Inspection procedures for crop seeds Other items inspection
GB8170 Numerical rounding rulesbZxz.net
3 Composition and operation procedures of inspection procedures for crop seeds Figure 3.1 Composition
The inspection procedures for crop seeds are composed of seven series of standards, including GB/T3543.1~3543.7. In terms of its content, it can be divided into three parts: sampling, testing and result reporting.
(Seed batch sampling procedure
Sampling part^Laboratory sampling procedure
Sample preservation
Clarity analysis (including determination of the number of seeds of other plants
)
Germination test
Authenticity and variety purity identification
Testing partMoisture determination
Biochemical determination of vitality
Weight determination
Seed health determination
Coated seed inspection
Approved by the State Administration of Technical Supervision on August 18, 1995 (see Chapter 4)
(see 5.1)
(see 5.2)
(see 5.3)
(see 5.4)
(see 5.5)
(see 5.5)||tt ||(See 5.5)
(See 5.5)
1996-06-01Implementation
【Tolerance
Result reportConditions for issuing result report sheetGB/T3543.1-1995
(See Chapter 6)
(See 7.2)
(Result report sheet
Among them, the purity analysis, germination test, authenticity and variety purity identification, and moisture determination of the test part are mandatory inspection items, and other inspection items such as biochemical determination of vitality are non-mandatory inspection items. 3.2 Seed inspection operation procedure diagram
The operation procedure to be followed during comprehensive inspection is shown in the figure below. Seed batch
Initial sample
Mixed sample
Sample for inspection
Test sample
|Samples for inspection
Test samples
Samples for inspection
Heavy impurities determination
Test samples
Purity analysis
Result report
Seed inspection procedure diagram
Retained samples
Samples for inspection
Test samples
Note: (1) The weights of the samples for inspection and the test samples in this diagram are different. Please refer to Articles 5.5.1 and 6.1 of GB/T3543.2. 2) Depending on the requirements of the measurement, the health measurement is sometimes carried out on clean seeds and sometimes on part of the samples for inspection. () If the number determination and purity analysis of seeds of other plants are to be carried out at the same time, the same sample for inspection can be used to perform purity analysis first and then determine the number of seeds of other plants.
4 Sampling part
The sample is randomly taken from a large number of seeds - a sample of appropriate weight and representative for inspection. The sample should be a small portion of seeds randomly taken from different parts of the seed batch, and then the sample is divided into samples of specified weight by bisection or random sampling. No matter which step, it must be representative. The specific sample method should comply with the provisions of GB/T3543.2. 22
5 Testing part
5.1 Purity analysis
CB/T3543.1—1995
Purity analysis is to determine the weight percentage of different components of the sample for inspection and the characteristics of the sample mixture, and to infer the composition of the seed batch based on this. During the analysis, the test sample is divided into three components: clean seeds, other plant seeds and impurities, and the weight percentage of each component is determined. All plant seeds and various impurities in the sample are identified as much as possible. For the convenience of operation, the number of other plant seeds is also included in the purity analysis. It is mainly used to determine whether the seed batch contains static or harmful seeds. It is expressed by the number of other plant seeds in the sample for inspection. If identification is required, it can be identified to the genus according to plant classification. The specific analysis should comply with the provisions of GB/T3543.3. 5.2 Germination test
Germination test is to determine the maximum germination potential of seed batches. Based on this, the quality of different seed batches can be compared and the field sowing value can be estimated. The germination test must be carried out with clean seeds after purity analysis, under suitable moisture and specified germination technical conditions. After the seedlings are suitable for evaluation, each repetition is checked according to the requirements of the result report, and different types of seedlings are counted. If pre-treatment is required, it should be indicated in the report.
The specific test method should comply with the provisions of GB/T3543.4. 5.3 Authenticity and variety purity identification
The authenticity and variety purity of the seeds of the sample to be tested are determined, and the authenticity and variety purity of the seeds of the seed batch are inferred from this. Authenticity and variety purity identification can be carried out using seeds, seedlings or plants. Usually, the seeds are compared with the seeds of the standard sample, or the seedlings and plants are compared with the seedlings and plants of the standard sample planted in the same period and at the same development stage under the same environmental conditions. When the identification characteristics of the variety are relatively consistent (such as self-pollinating crops), the seeds, seedlings or plants of different crops and varieties are counted; when the identification characteristics of the variety are less consistent (such as cross-pollinating crops), the obvious variants are counted and an overall evaluation is made. The specific method should comply with the provisions of GB/T3543.5. 5.4 Moisture determination
The moisture content of the seeds of the sample to be tested is determined to provide a basis for safe storage and transportation of seeds. The seed moisture determination must remove all free water and bound water in the seed moisture, and at the same time minimize the loss of oxidation, decomposition or other volatile substances.
The method shall comply with the provisions of GB/T3543.6. 5.5 Other Items Inspection
5.5.1 Biochemical (tetrazolyl) Determination of Vitality
When it is urgent to know the seed germination rate in a short period of time or when some samples still have a large number of dormant seeds at the end of germination, the biochemical method of vitality can be used to quickly estimate the seed vitality.
The vitality determination uses a colorless solution of 2,3,5-triphenyltetrazolium chloride (abbreviated as tetrazolyl, TTC) as an indicator. After being absorbed by the living tissue of the seed, this indicator accepts hydrogen in the dehydrogenase of the living cells and is reduced to a red, stable, non-diffusible and water-insoluble phenylformamide. Based on this, the staining reaction of the embryo and endosperm tissue can be used to distinguish between seeds with vitality and seeds without vitality. In addition to studying fully dyed viable seeds and completely undyed inactive seeds, the viability of partially dyed seeds is mainly determined by the location and area of necrotic tissue in the endosperm. The depth of dyeing color can determine whether the tissue is healthy, weak or dead.
5.5.2 Weight determination
Determine the weight of each 1,000 seeds of the sample to be tested. Take a certain number of seeds from the net seeds, weigh them, calculate the weight of 1,000 seeds, and convert them into the weight under the moisture conditions specified in the national seed quality standard.
5.5.3 Seed health determination
Through the health determination of the sample, the health status of the seed batch can be inferred, so as to compare the use value of different seed batches. At the same time, measures can be taken to make up for the shortcomings of the germination test. GB/T 3543.1-1995
According to the requirements of the tester, determine whether the sample contains pathogens and pests, and use appropriate methods to estimate the number of infected seeds as much as possible. For batches of seeds that have been treated, the tester should be asked to explain the treatment method and the chemicals used. 5.5.4 Coated seed inspection
Coated seeds refer to various treated seeds that use a certain method to wrap other non-seed materials outside the seeds. Including pelleted seeds, coated seeds, seed belts and seed blankets. Since it is difficult to directly test coated seeds according to the methods specified in GB/T3543.2~~3543.6, in order to obtain results with reproducible sowing value for coated seeds, corresponding regulations are made. With.The specific test methods of the above contents (5.5.1 to 5.5.4) shall comply with the provisions of GB/T3543.7. 6 Tolerance
Tolerance refers to the maximum difference allowed between the results of two tests of the same test item. Exceeding this limit is sufficient to cause doubts about the accuracy of the results or to believe that there are real differences in the conditions of the test. 6.1 The allowable difference between the repeated tests of the same sample in the same laboratory. Clarity analysis (see Table 2 of GB/T3543.3). #
b. Determination of the number of seeds of other plants (see Table 6 of GB/T3543.3). Germination test (see Table 3 of GB/T3543.4). c
d. Viability determination (see Table 2 of GB/T3543.7). 6.2 The same or different samples taken from the same seed batch shall be tested by the same or another testing agency to compare whether the two results are consistent. a. Clarity analysis (see Table 3 of GB/T3543.3). b. Determination of the number of seeds of other plants (see Table 6 of GB/T3543.3). C. Germination test (see Table 5 of GB/T3543.4). 6.3 The second sample sent for inspection from the same seed lot is inspected by the same or another inspection agency, and the result obtained is worse than the first one, such as low net seed weight percentage, low germination rate, and high number of seeds of other plants. a. Purity analysis (see Table 4 of GB/T3543.3). b. Determination of the number of seeds of other plants (see Table 7 of GB/T3543.3). Germination test (see Table 4 of GB/T3543.4). c.
6.4 Comparison of random inspection, unified inspection, arbitration inspection, regular inspection, etc. with the specified values of seed quality standards, contracts, labels, etc. Purity analysis (see Table 5 of GB/T3543.3). a.
Germination test (see Table 6 of GB/T3543.4). b.
Purity certification (commercial varieties, see GB/T3543.5 Table 2). Purity identification (breeder, original seeds, etc., see GB/T3543.5 Table 3). d.
7 Result report
The seed inspection result sheet is a certificate form for the inspection results obtained by sampling and testing in accordance with this standard. 7.1 Conditions for issuing the result report
In addition to filling in the inspection items, the institution that issues the seed inspection result sheet must also: the institution is currently engaged in this work;
The inspected variety belongs to a variety listed in this regulation; b.
The seed batch is in compliance with the requirements specified in this regulation; d. The samples sent for inspection are taken and processed in accordance with the requirements of this regulation; e
The inspection is carried out in accordance with the methods specified in this regulation. 7.2 Result Report
After the test items are completed, the test results should be reported on the seed test result report form (see the table below) in accordance with the relevant provisions of the result calculation and result reporting in GB/T3543.3~3543.724
GB/T3543.1--1995
. If some items are not measured and the result report form is blank, then the words "not tested" should be filled in these blanks.
Seed inspection result report
Inspection unit
Crop name
Variety name
Net seeds, %
Types and numbers of other plant seeds:
Types of impurities:
Normal seedlings%
Germination bed
Laboratory method
Field plot identification
Vitality
Weight (dry grain)
Health status:
Inspection unit (stamp):
Hard and solid%
Representative quantity
Other plant seeds, %
Impurities, %
Complete/limited/simplified inspection
Fresh non-germinated seeds%!
Abnormal seedlings%
, test duration
+variety purity
This variety
Inspector (technical person in charge):
%;Foreign variety
Reviewer:
Dead seeds%
Pre-germination treatment and method
Filling date:
Year-month-day
If the sampling is conducted by another inspection agency or individual, it should be noted on the result report that it is only responsible for the samples submitted for inspection. If it is urgent to know the results of a certain measurement item before the end of the inspection, a temporary result report can be issued, that is, the result report will be attached with the statement "The final result report will be issued at the end of the inspection". If this regulation does not provide for and requires numerical rounding, the provisions of GB8170 shall be implemented. The complete result report sheet shall report the following contents:.
The name of the issuing station,
The name of the sampling and sealing unit;
The official mark and seal of the seed batch;
The number of samples and the number of representatives;
The sampling date;
The date when the inspection station received the sample;
The sample number;
The inspection item;
The inspection date.
The result report sheet shall not be altered.
Additional remarks:
GB/T 3543.1—1995
This standard was proposed by the Ministry of Agriculture of the People's Republic of China. This standard is under the jurisdiction of the National Technical Committee for Standardization of Crop Seeds. This standard was drafted by the National Seed Station, Zhejiang Agricultural University, Sichuan Province, Heilongjiang Province, Tianjin Seed Company (Station), Nanjing Agricultural University, Beijing, and Hunan Seed Company. The main drafters of this standard are Zhi Juzhen, Bi Xinhua, Du Kemin, Chang Xiulan, Yang Shuhui, Ren Shuping, Wu Zhixing, Li Renfeng and Zhao Juying. This standard was first issued in March 1983.
This standard adopts the International Seed Inspection Procedure (ISTA, 1993 edition) with reference to it. 261--1995
Fill in the seed inspection result report form (see the table below). If some items are not tested and the result report form is blank, then fill in the words "not tested" in these blanks.
Seed inspection result report
Inspection unit
Crop name
Variety name
Net seeds, %
Types and numbers of other plant seeds:
Types of impurities:
Normal seedlings%
Germination bed
Laboratory method
Field plot identification
Vitality
Weight (dry grain)
Health status:
Inspection unit (stamp):
Hard and solid%
Representative quantity
Other plant seeds, %
Impurities, %
Complete/limited/simplified inspection
Fresh non-germinated seeds%!
Abnormal seedlings%
, test duration
+variety purity
This variety
Inspector (technical person in charge):
%;Foreign variety
Reviewer:
Dead seeds%
Pre-germination treatment and method
Filling date:
Year-month-day
If the sampling is conducted by another inspection agency or individual, it should be noted on the result report that it is only responsible for the samples submitted for inspection. If it is urgent to know the results of a certain measurement item before the end of the inspection, a temporary result report can be issued, that is, the result report will be attached with the statement "The final result report will be issued at the end of the inspection". If this regulation does not provide for and requires numerical rounding, the provisions of GB8170 shall be implemented. The complete result report sheet shall report the following contents:.
The name of the issuing station,
The name of the sampling and sealing unit;
The official mark and seal of the seed batch;
The number of samples and the number of representatives;
The sampling date;
The date when the inspection station received the sample;
The sample number;
The inspection item;
The inspection date.
The result report sheet shall not be altered.
Additional remarks:
GB/T 3543.1—1995
This standard was proposed by the Ministry of Agriculture of the People's Republic of China. This standard is under the jurisdiction of the National Technical Committee for Standardization of Crop Seeds. This standard was drafted by the National Seed Station, Zhejiang Agricultural University, Sichuan Province, Heilongjiang Province, Tianjin Seed Company (Station), Nanjing Agricultural University, Beijing, and Hunan Seed Company. The main drafters of this standard are Zhi Juzhen, Bi Xinhua, Du Kemin, Chang Xiulan, Yang Shuhui, Ren Shuping, Wu Zhixing, Li Renfeng and Zhao Juying. This standard was first issued in March 1983.
This standard adopts the International Seed Inspection Procedure (ISTA, 1993 edition) with reference to it. 261--1995
Fill in the seed inspection result report form (see the table below). If some items are not tested and the result report form is blank, then fill in the words "not tested" in these blanks.
Seed inspection result report
Inspection unit
Crop name
Variety name
Net seeds, %
Types and numbers of other plant seeds:
Types of impurities:
Normal seedlings%
Germination bed
Laboratory method
Field plot identification
Vitality
Weight (dry grain)
Health status:
Inspection unit (stamp):
Hard and solid%
Representative quantity
Other plant seeds, %
Impurities, %
Complete/limited/simplified inspection
Fresh non-germinated seeds%!
Abnormal seedlings%
, test duration
+variety purity
This variety
Inspector (technical person in charge):
%;Foreign variety
Reviewer:
Dead seeds%
Pre-germination treatment and method
Filling date:
Year-month-day
If the sampling is conducted by another inspection agency or individual, it should be noted on the result report that it is only responsible for the samples submitted for inspection. If it is urgent to know the results of a certain measurement item before the end of the inspection, a temporary result report can be issued, that is, the result report will be attached with the statement "The final result report will be issued at the end of the inspection". If this regulation does not provide for and requires numerical rounding, the provisions of GB8170 shall be implemented. The complete result report sheet shall report the following contents:.
The name of the issuing station,
The name of the sampling and sealing unit;
The official mark and seal of the seed batch;
The number of samples and the number of representatives;
The sampling date;
The date when the inspection station received the sample;
The sample number;
The inspection item;
The inspection date.
The result report sheet shall not be altered.
Additional remarks:
GB/T 3543.1—1995
This standard was proposed by the Ministry of Agriculture of the People's Republic of China. This standard is under the jurisdiction of the National Technical Committee for Standardization of Crop Seeds. This standard was drafted by the National Seed Station, Zhejiang Agricultural University, Sichuan Province, Heilongjiang Province, Tianjin Seed Company (Station), Nanjing Agricultural University, Beijing, and Hunan Seed Company. The main drafters of this standard are Zhi Juzhen, Bi Xinhua, Du Kemin, Chang Xiulan, Yang Shuhui, Ren Shuping, Wu Zhixing, Li Renfeng and Zhao Juying. This standard was first issued in March 1983.
This standard adopts the International Seed Inspection Procedure (ISTA, 1993 edition) with reference to it. 26
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