Guidelines for crop safety evaluation of pesticides--Part 2:Laboratory test for crop safety of photosynthesis-inhibiting herbicides
Some standard content:
ICS 65.100
Agricultural Industry Standard of the People's Republic of China
NY/T1965.2--2010
Guidelines for crop safety evaluation of pesticides-Part 2: Laboratory test for crop safety of photosynthesis-inhibiting herbicides2010-12-23 Issued
2011-02-01 Implementation
Ministry of Agriculture of the People's Republic of China
NY/T1965.2—2010
NY/T1965 "Guidelines for Crop Safety Evaluation of Pesticides" is a series of standards. Part 1: Indoor test methods for crop safety evaluation of fungicides and germicides; Part 2: Test methods for crop safety determination of photosynthesis-inhibiting herbicides. This part is the second part of NY/T1965. This part was drafted in accordance with the rules given in GB/T1.1-2009. This part was proposed and managed by the Crop Management Department of the Ministry of Agriculture of the People's Republic of China. The drafting units of this part are: Pesticide Control Institute of the Ministry of Agriculture, Institute of Cereals and Oil Crops of Hebei Academy of Agricultural Sciences. The main drafters of this part are: Zhang Jia, Zheng Guiqi, Zhang Hongjun, Xu Xian, Zhang Wenjun, Liu Xue, Zhou Xinxin. T
1 Scope
Criteria for the evaluation of the safety of pesticides to crops
NY/T1965.2-2010
Part 2: Test methods for the determination of the safety of photosynthetic inhibitory herbicides to crops This part specifies the basic requirements and methods for the determination of the safety of photosynthetic inhibitory herbicides to crops. This part applies to the indoor test and evaluation of the safety of photosynthetic inhibitory herbicides for crop registration. 2 Instruments and Equipment
2.1 Lighting incubator: light intensity 0[x～30000[x, temperature 10C～50C, humidity 50%~～95%; or a controllable solar greenhouse that can meet the above light intensity, temperature and humidity requirements. 2.2 Controllable quantitative spray equipment: spray volume 30mL/m2~~60mL/in2.3 Electronic balance: shake volume 0.1mg.
2.4 Pipette or pipette, etc.
3 Test Materials
3.1 Soil
Use sieved (60 mesh) air-dried sandy loam with an organic matter content of 1%~4%, neutral (pH 6.0~～8.0), and good permeability, and measure and record the soil organic matter content and pH
3.2 Crops
Select the test crop types according to the type of herbicide, and record the crop varieties and seed sources. The seed quality of crops should not be lower than the corresponding national standard level 2 requirements.
3.3 Weeds
Select representative sensitive weeds that are easy to cultivate and have the same growth period, and their seed germination rate should be above 80%. Common weeds include: Digitaria sunguinatis, Setaria viridis, Echinochtoa crus-galli, Eleusine indica, Aloperurus aequalix, Alopecurus japonicus, Avenfatua, Polypogrynfugax, Beckmannia syzigachne, Poa annua, Abhuti-lon tfieuphrasti, Anaranthus retrofteaus, Portulaca aleraceu, Chenopodium at-bum, Rumercrispus, Commelina communis, Xanthium strumarium, Sotunum nigrum, Stellarius tnedia), Malachium aquaticum, Galium apa-rine, Vermica didym, Erigerunannuus, Vicin satiua, Descuruiniasophia, Cyperusiria, Cyperusdifformis, Junceltusserotinus, Cyperusrotundus, Leptochloachinensis, Paspatumdistichun, Eclipta prostrata, Monocharia vaginalis, Suagittaria trifutia, Lenna mi-20r, etc.
3.4 Agents
3.4.1 Test Agents
Original drug (mother drug) or preparation, indicate the manufacturer and production period or batch. 3.4.2 Control Agents
NY/T1965.2—2010
Use the original drug (mother drug) or preparation of pesticides that have been registered and commonly used in production. Its chemical structure type or mode of action should be the same or similar to that of the test agent.
4 Test Methods
4.1 Test Material Preparation
4.1.1 Crops
Fill the test soil to 4/5 of the pot, and soak the soil from the bottom to make it completely moist. When the test agent is a soil treatment agent, evenly sow 20 seeds of the test crop on the surface of the soil, 0.5cm~2. 0cm according to the seed size, and set aside 24 hours after sowing. When the test agent is a stem and leaf treatment agent, the test crop seeds are evenly spread on the soil surface. According to the seed size, cover the soil with 0.5cm~2.0cm, and move to the greenhouse or light culture box for routine cultivation after sowing. After the crop emerges, thin out the seedlings and set the plants, and keep 10 plants in each pot. According to the characteristics of the agent, select the test materials of the appropriate period for use. Early field crops are watered by percolation irrigation at the bottom of the pot, and paddy field crops (water dams) are watered to saturation by irrigation at the top of the pot. According to the application method, select the appropriate test material cultivation method. 4.1.2 Weeds
Cultivate in the same way as the test crops and set aside. 4.2 Preparation of agents
Dissolve the water-soluble technical in distilled water, and other technical in a suitable solvent (acetone, dimethylformamide or dimethyl sulfoxide, etc.) and dilute with 0.02%~~0.1% Tween 80 aqueous solution. The preparation is directly diluted with water. Each test agent and control agent has 5 to 7 series of agent scenes.
4.3 Agent treatment
According to the test requirements, select the spray equipment parameters such as spray pressure, nozzle height, spray volume and nozzle model, and perform soil spray or stem and leaf spray treatment in sequence from low dose to high dose. Each treatment shall be repeated at least 4 times, and a treatment without agent shall be set as a blank control. Record the dynamic data of temperature and humidity in the greenhouse or light incubator during the test. 4.4 Result investigation
After treatment, observe and record the growth status of crops and weeds regularly. At 11d or 21d, use visual inspection and growth inhibition rate survey results to investigate the effects of the agent on crops and weeds. The investigation time can be adjusted according to the test requirements and agent characteristics. 5 Visual inspection results and description
5. 1 Symptoms of damage
Color changes: yellowing, bleaching, purple, scorching, etc.; Morphological changes: leaf deformity, stem and leaf twisting, curling, etc.; Growth changes: dehydration, braiding, and stalk growth, etc. 5.2 Damage degree
5.2.1 Crops
According to the damage symptoms and severity of the test crops, safety evaluation is carried out. The following unified methods can be used for investigation: Safety, no pesticide damage: The degree of pesticide damage is 0:
Minor pesticide damage: The degree of pesticide damage is 1%~10%;
Moderate pesticide damage: The degree of pesticide damage is 11%~30%; Severe pesticide damage: The degree of pesticide damage is 31%~50%;
Severe pesticide damage: The degree of pesticide damage is >50%,
5.2.2 Weeds
NY/T 1965.2—2010
Evaluate the herbicidal activity of the pesticide according to the symptoms and severity of the target weeds. The following classification method can be used for investigation:
Grade 1: All dead;
Grade 2: Equivalent to 0%~~2.5% of the weeds treated with the blank control; Grade 3: Equivalent to 2.6%~5% of the weeds treated with the blank control; Grade 4: Equivalent to 5.1%~10% of the weeds treated with the blank control; Grade 5: Equivalent to 10.1%~15% of the weeds treated with the blank control; Grade 6: Equivalent to 15.1%~25% of the weeds treated with the blank control; Grade 7: Equivalent to 25.1%~~35% of the weeds treated with the blank control; Grade 8: Equivalent to 35.1%~67.5% of the weeds treated with the blank control; Grade 9: Equivalent to 67.6%~~100% of the weeds treated with the blank control; Grade 10: Equivalent to 10.1%~15% of the weeds treated with the blank control; Grade 11: Equivalent to 10.1%~25% of the weeds treated with the blank control; Grade 12: Equivalent to 25.1%~~35% of the weeds treated with the blank control; Grade 13: Equivalent to 35.1%~67.5% of the weeds treated with the blank control; Grade 14: Equivalent to 67.6%~~100% of the weeds treated with the blank control; Grade 15: Equivalent to 10.1%~15% of the weeds treated with the blank control; Grade 16: Equivalent to 10.1%~25% of the weeds treated Data statistics and analysis
6.1 Calculation methodbZxz.net
6.1.1 Crop growth inhibition rate
The inhibition rate R of the crop fresh weight (or plant height) of each treatment is expressed as mass fraction (%) and calculated according to formula (1): R=X=Xi×100
X——fresh weight of the crop (or plant height), in grams or centimeters (g or ctm); X——fresh weight (or plant height) of the treated crop, in grams or centimeters (g or cm). The calculation result is retained to two decimal places.
6.1.2 Weed growth inhibition rate
The control effect E of the fresh weight (or number of plants) of the weeds of each treatment is expressed as mass fraction (%) and calculated according to formula (2): Te=T×100
Where:
T. ——Fresh weight of the aboveground part of the control weeds (or the number of weeds), in grams or plants (or plants): T-Fresh weight of the aboveground part of the treated weeds (or the number of weeds), in grams or plants (g or plants). The calculation results are kept to two decimal places.
6.1.3 Selectivity index
Calculate the inhibition percentage of the fresh weight or plant (number of plants) of the test crops and weeds by each treatment, and calculate the ED of the test herbicide on weed growth inhibition and the ED of the test herbicide on crop growth inhibition by regression analysis. The selectivity index I is calculated according to formula (3):
W:
C--EDIc of crops;
W--EDIc of weeds.
The calculation results are kept to two decimal places.
6.2 Statistical analysis
Use DPS, SAS or SPSS standard statistical software to conduct regression analysis on the logarithmic value of the dosage of the pesticide and the probability value of the inhibition rate, and calculate the crop ED value and 95% confidence limit and the weed ED% value and 95% confidence limit. 3
NY/T1965.2—2010
Results and report writing
Analyze and evaluate the statistical results, write a formal test report, list the original data, and attach photos of the symptoms of pesticide damage. 4
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