SC/T 1057-2002 Technical specification for transplantation and proliferation of whitebait Transplantation and proliferation of whitebait
Some standard content:
ICS 67. 120. 30
Aquatic Industry Standard of the People's Republic of China
SC/11057—2002
Technical specification for transplantation and enhancement of salangids-Technigues for transplantation andcnhancement of largeicefish
Published on 2002-11-05
Ministry of Agriculture of the People's Republic of China
Implementation on 2002-12-20
"Technical specification for transplantation and enhancement of salangids-Technigues for transplantation andcnhancement of largeicefish" series of standards are divided into two independent standards: -----SC/T1057 "Technical specification for transplantation and enhancement of salangids-Technigues for transplantation andcnhancement of largeicefish"; -S/T1058 "Technical specification for transplantation and enhancement of salangids-Technical specification for transplantation and enhancement of largeicefish in Taihu Lake" Appendix A of this standard is a normative appendix.
This This standard is issued by the Fisheries Bureau of the Ministry of Agriculture. bZxz.net
This standard is under the jurisdiction of the Freshwater Aquaculture Sub-Technical Committee of the National Technical Committee for Standardization of Aquatic Products. SC/T10572002
This standard is drafted by: Freshwater Fisheries Research Center of the Chinese Academy of Fishery Sciences, Fisheries Bureau of the Inner Mongolia Agricultural Department. Drafters of this standard: Shi Weigang, Zhou Xin, Sun Xiaowen, Liu Wei. 1 Scope
Technical specifications for transplantation and proliferation of silverfish
Transplantation and proliferation technology of large silverfish
SC/T1057—2002||tt| |This standard specifies the environmental conditions for the transplantation of Pasaianyuranis (Ahint), the preparation of fertilized eggs and fry, quality determination and transportation, and the management of stocking and proliferation. It is used for the transplantation, proliferation and reproduction of large silver carp.
2 Normative references
The clauses in the following documents become the clauses of this standard through reference in this standard. For any dated referenced document, all subsequent amendments (excluding those made in the survey) or revisions are not applicable to this standard. However, the parties to the agreement based on this standard are encouraged to Study whether the latest versions of these documents are available. For any referenced documents that are not in use, their latest versions shall apply to this standard. GB11607 Industrial Water Quality Standard
GB/115806 Method for calculating the fertilization rate of eggs of carp, grass carp, silver carp and bighead carp 3 Environmental conditions for transplantation waters
3.1 Abiotic factors
3-1.1 Lakes and reservoirs north of 28°N in my country are suitable areas for transplantation. 3.1.2 Water temperature range for growth 0℃-32℃ Suitable temperature for spawning 1℃～~5℃ 3.1.3 Area of suitable water bodies for transplantation 100h or more 3.1.4 The water depth should be above 1m, and the bottom should be hard and gravel bottom. 3-1.5 The water quality should meet the requirements of GB311607. The transparency of the water body in the water should be 20rm~-300cm+pH %.5~-5.2. The radiometric index should be less than 5.
3.2 Biological factors
3.2.7 It is required that the fish fauna is simple and there are vacant ecological niches in the middle and upper water layers. 3.2.2 Carnivorous species in the middle and upper water layers such as perch, horse mouth angler, sugar angler, etc. are the main enemies of big silver angler. 3-2.3 The main bait competitors of silver fish in the early stage of growth are some fish that mainly feed on zooplankton throughout their lives, such as: Zhanyu, surface spike angler, smart stripe, male fish, fragrant color, Taihu petal fish, etc. 3.2.4 The potassium feed of silver fish in the early stage is zooplankton, and the zooplankton in the spring of transplanted water bodies is not less than 1.0mg/1. In the middle and late stages, the bait is mainly small species and shrimps, such as fish, wheat sugar fish, Taihu new silver fish, and white shrimp. 4 Preparation of fertilized eggs
4.1 Selection of broodstock
4.1.1 Mature broodstock are those caught during the peak breeding period of whitebait in lakes and reservoirs: 4.1.2 The abdomen of sexually mature female fish is full and soft, and the eggs are fully fertilized. When the abdomen is lightly pressed, the eggs can be seen flowing out of the cloaca. The head and sides of the genitals of sexually mature fish are dark red. The anus is large and fan-shaped, and there is a row of reproductive scales with strong adsorption on the anal fin. The testes are located at the back of the abdomen. The sperm is crescent-shaped, and the sperm is milky white and spreads out in the water. 4.1.3 The body length of broodstock should be greater than 110.
4.1.4 After being caught, the broodstock should be stored in a drain, and the surface should be slightly covered with wet gauze to prevent wind, sun, cold and squeezing. When the temperature is 1 (-3°C), the temporary storage time should not exceed 1 hour. In cold areas, it can be extended to 4 hours to -5 hours. Artificial insemination should be completed as soon as possible during the temporary storage time. 4.2 Artificial insemination
4.2.7 Suitable water temperature for insemination is 1°C -5 C.4.2.2 The ratio of male to female is 1:1.
4.2.3 During artificial insemination, use your thumb and index finger to gently lift the head of the female rabbit, wipe off the water and dirt on the female body, and do not use your thumb to push the female body! Slowly push the abdomen downward and squeeze the eggs into the cloaca. Then quickly pick up the male fish, aim the knife at the male fish nest, cut it and let the white semen flow out. Immediately use a rubber suction ball to absorb clean water to rinse the male semen on the eggs, and use the tail of the cockroach to mix the semen and eggs while rinsing. Continue to stir 10g-15-2cm. Add new water to exceed the surface of the egg layer. After standing for 1min~2ii, put it in a container for temporary storage. After cleaning and rinsing, the fertilized eggs can be sent to the incubator for incubation. 4.2.4 Calculation of fertilization rate according to GB/T15806 The regulations are to be implemented. 4.3 Quality requirements for fertilized eggs
4.3.1 Observed with the naked eye, the fertilized eggs are approximately shaped like a synovium, with a crystal clear color, full and sinking, and slightly adsorbent. 4.3.2 Observed under a dissecting microscope, the fertilized eggs have smooth membranes, with evenly distributed oolemma fibers, clear perivitelline, and different phases of metamorphosis, zygote, liver and fetal development. See Appendix A.
4.3.3 Fertilized eggs of high blastocyst and later stages. After being allowed to settle for 3 minutes, the number of fertilized eggs per milliliter is (1250-250) 4.3.4: 05% of the metamorphosis stage sperm should be sent to 0.8ml or 1 5 Preparation of fry
5.1 Hatching of fertilized eggs
5.1.1 Hatching rate: ventilation, facing the sun and avoiding direct sunlight. 5.1.2 Suitable water temperature for hatching: 2C~8C
5.1.3 Water quality: in accordance with the provisions of GB11607. 5.1.4 The hatching container should be a glass aquarium with a height of more than 50cm and a water depth of 30cm35cm. The density of eggs is 3×10°/m. Change the water twice a day. Stir the water thoroughly before changing the water until the eggs float up. Change the water when the eggs sink. The amount of water changed each time is one-fourth:. The water temperature change range is ±1. 5 C.
5.1.5 In the appropriate incubation temperature range, lowering the water temperature or appropriately raising the temperature can effectively control the incubation period. Aerating until the eggs are slightly turned and increasing the light intensity (not exceeding the outdoor light intensity) can accelerate the embryonic development process. Under normal indoor light, when the incubation water temperature is 2C~8C without aeration, the fry will emerge in 50 days-60 days.
5.2 Incubation management
5.2.1 There should be a dedicated person on duty. After each water change, it should be ensured that there is no accumulation of fertilized eggs. During the incubation process, the dead eggs that have been fertilized and fertilized should be removed in time. The development of fertilized eggs should be checked regularly with a dissecting microscope (see Appendix A). 5.2.2 If the fertilized eggs are infected with water strawberry, they can be soaked in 2×10-concentration high-purity potassium for 5min~10min. Then rinse with clean water until they are clean.
6 Fertilized eggs, fry transportation
6. 1 Fertilized eggs and fry counting
6.1.1 When sampling and counting fish eggs, stir the water in the egg storage container to make the eggs evenly distributed in the water: every 5tml, randomly sample 3ml-4ml of the sample in a measuring tube. Let it stand for 3m to allow the eggs to fully settle, read the milliliters of the volume of the fish eggs, count the gold-colored eggs in the measuring tube, and obtain the number of eggs sampled for the first time. Repeat the counting of fish egg bundles twice and calculate the average number per liter by weight. 6.1.2 To calculate the total amount of fertilized eggs, take the eggs to be tested in a larger single cylinder. Let it stand for 3 minutes, read the volume occupied by the egg, and multiply it by 2. The number of fish eggs per milliliter and the fertilization rate obtained from the sampled eggs is the total amount of fertilized eggs: SC/T10572002
6.1.3 When sampling and counting fry, stir the water in the container slowly to make the fry evenly distributed. Randomly sample 3ml~1mI with a 5mL measuring cylinder. Read the milliliters of the water sample in the collected volume, and use a pipette to suck all the fish eggs in the measuring cylinder for counting. Repeat the sampling and counting times to find the average number of fry per milliliter of water sample. 6.1.4 Total number of fry. The water in the fry container should be stirred slowly to make the fry evenly distributed in the water. Take a large amount of fry to be tested, read the number of milliliters of the fry-containing water sample, and multiply it by the number of fry per milliliter obtained by sampling to get the total number of fry. 6.2 Packaging and transportation of fertilized eggs and fry
6.2.1. The water composition for transporting fertilized eggs and fry shall comply with the provisions of GB11607. 6.2.2. Suitable water temperature for transporting fertilized eggs is (4-3). It is best to transport fertilized eggs after they have developed to the high blastocyst stage. If they can reach the transfer and release site within half a day, they can be transported in plastic barrels: the water volume is the container; 1. 3000-5000 fertilized eggs can be loaded in 1 liter of water. If it takes more than half a day to reach the transfer and release site, they must be packed in double-layered ethylene plastic bags and sealed with oxygen. The water volume should be no more than one-fourth of the plastic bag volume, and the oxygenated volume should account for about one-fourth. 10,000-20,000 fertilized eggs can be loaded in each liter of water. 6.2.3. Suitable water temperature for transporting fry is (7-2)℃: 7-10℃. It takes 7 days to 10 days from hatching to swimming. After the water level drops, the fish can be packed in double-layer vinyl plastic bags and filled with oxygen for transportation. The number of fry per liter of water is about 500. If the distance of the ship is short, the number can be increased to 8,000-10,000 per liter of water. The water volume accounts for one ninth of the volume of the plastic bag, and the oxygen volume accounts for about three fifths. 7. Release of fertilized eggs and fry
7.1 Release of fertilized eggs
7.1.1 The release site should be selected in a sunny place with leeward wind and a water depth of 1, (tm1-2.5 m, the water level is stable, the bottom is sandy or hard mud, and the bait is very rich in waters. Avoid multiple placement.
7.1.2 When placing, the container should be placed in the water body of the transplanting site. After the water temperature in the container is consistent with the water temperature in the transplanting site, the fertilized eggs should be slowly placed in the transplanting water body.
7.1.3 The density of fertilized eggs is (100 ~ 1000) grains/hm. If the water area is less than 200hm, the density can be increased to (9nm ~ 1100) grains/hm.
7.2 The placement of fish fry
The placement location and method shall be carried out in accordance with the provisions of 7.1. "and 7.1.2. The stocking density is 1500 tails/hrm~3000 tails /hm2. If the water area is less than 2000 t/hm2, the stocking density can be increased to 5000 t/hm2. 8.1 Strengthen fishery administration
Conscientiously implement the Fisheries Law of the People's Republic of China, strictly prohibit illegal operations such as electrocution, poisoning and blasting, 8.? Establish breeding protection facilities
Designate 5000 to 1000 t of water area as a seasonal protection area, focusing on protecting spawning grounds. Determine the beach ban period, implement seasonal equalization of lakes during the growth stage of young tuna, strengthen resource monitoring, focus on monitoring growth, resource density, bait and predators, make monitoring records, and take relevant technical and management measures.
8.3 Make a good fishing plan
8. 3.1 Estimate the existing stock of resources according to resource density, and formulate a plan for the maximum and surplus fishing. 8.3-2 Adjust the intensity of gathering in a timely manner, adjust the fishing plan according to the dynamics of resource density, and make timely adjustments to the number of vessels, horsepower, nets, etc. When the amount of resources decreases significantly, prohibitive measures should be taken, and eggs or seedlings should be replenished in time for release and reproduction. 3
SC/T 1057—2002
Thanks to A
(Normative Appendix)
The different phases of embryonic development of fertilized eggs of whitebait are very different. See Figure A.1 Figure A.14, the different phases of embryonic development of fertilized eggs are shown in Figure A.1, Figure A.14, the different phases of embryonic development of fertilized eggs are shown in Figure A.1, Figure A.2, the high blastocyst stage, Figure A.5, Figure A.3, the B cell, Figure A.6, the 16 cell, Figure A.4, the 4 Cell
Figure A, 764 Cell
Figure A.8 Blastocyst stage
Figure A.11 Somite emergence stage
Bacteria A.9 Gastrula stage
Figure 4.12 Muscle effect stage
Figure 4.14 Whitebait fry
SC/T 1057 2002
Figure A: 10 Neuraminid stage
Emergence stage
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