This part specifies the methods and basic requirements for field efficacy plot tests of fungicides for the control of sugarcane pineapple disease (Ceratocystisparadoxa). This part applies to field efficacy plot tests and efficacy evaluation of fungicides for the control of sugarcane pineapple disease for registration. GB/T 17980.101-2004 Guidelines for field efficacy tests of pesticides (II) Part 101: Efficacy tests of fungicides for the control of sugarcane pineapple disease GB/T17980.101-2004 Standard download decompression password: www.bzxz.net
This part specifies the methods and basic requirements for field efficacy plot tests of fungicides for the control of sugarcane pineapple disease (Ceratocystisparadoxa). This part applies to field efficacy plot tests and efficacy evaluation of fungicides for the control of sugarcane pineapple disease for registration.

GR/T17980.101—2304
National pesticide efficacy standard is one of the important contents of agricultural product management, and it is an important basis for formulating agricultural product labeling. In order to regulate the methods and contents of pesticide field testing more scientific and standardized, and to make my country's efficacy test reports have international recognition, my country has formulated national standards for field efficacy test criteria. This series of standards has considered the field efficacy test criteria of the European and Pacific Regional Organization for the Protection of Marine Animals (EPP) and the standards of the United Nations Food and Agriculture Organization (FAO), and has been revised and formulated after a large number of field efficacy tests. Gansu mulberry disease is one of the most important diseases in my country. Cattle farmers often need pesticides for prevention and treatment. In order to determine the best use of pesticides for treating mulberry disease, test the effects of pesticides on crops and non-target beneficial organisms, and provide a basis for the efficacy evaluation and safe and reasonable use of registered fungicides, this part of T1FG is specially determined. Part II of the series of standards for the test of pesticide efficacy is an independent part. This part was issued by the Ministry of Agriculture of China. The unit that initiated this part is the Institute for the Control of Pesticides, Ministry of Agriculture. The authors of this part are Liu Wanchi, Gong Hengchong, Feng Baoyin, Shi Xinping, Li Changfang, Lu Zuhai, and Zhang Shinian. This part is under the supervision of the Pesticide Inspection Institute of the Ministry of Agriculture. 1. Scope: Field efficacy test criteria (II) Part 01: Fungicide control of sweet pear disease GB/T17980.101-2C04 This part specifies the requirements for the daily efficacy of fungicides to prevent blue pear disease (Ctocvssisraaa) in small plots. This part is applicable to field efficacy of fungicides for preventing vegetable dwarfism disease in small plots. 2. Experimental conditions
2.1 Experimental objects. Selection of crops and varieties. The experimental objects are sugarcane. The experimental crop is sugarcane. It should be a sensitive variety. Double-bud seedlings should be collected and the variety name should be recorded. 2.2 Environmental conditions
The test should be carried out in the field where the disease has been more serious in previous years. All the cultivation conditions (such as soil type, irrigation, water supply, row spacing, etc.) of the test should be consistent and in line with the local agricultural practice (GAP). Experimental design and arrangement
3.1 Chemicals
3.1.1 Experimental chemicals
Please note the trade name or code, general name, product name, dosage form and manufacturer of the chemical. The three doses of the test drug shall be treated according to the pre-drug basis stipulated in the test agreement (the test agreement signed by the test entrusting authority and the test contractor). 3.1.2 Control period
For the subsequent preparation of the drug, if the drug has been proven to have a good efficacy in actual combat, the type of test drug and the method of operation should be the most reliable. Special circumstances may be modified according to the test. 3.2 Plot arrangement
3.2.1 Plot arrangement
For the test drug, the plot arrangement shall be randomized before the initial control, and any special circumstances shall be explained. 3.2.2 Area and preparation of plots
Plot area, 15m2~.5um
Total number of times: not less than 1 time,
3.3 Method of application
3.3.1 Method of use
Follow the requirements of the agreement and the instructions on the label: usually, soak the 20-minute spray in the spray for 5 minutes: spraying should be adapted to local agricultural practices
3.3.2 Type of equipment used
Select the type of equipment commonly used in production, and remember to use the type of equipment used. The equipment used should be as close to the design as possible. 3.3.3 Time and number of application
Follow the instructions on the agreement label, usually before sowing. 1
GB/T17980.101—2004
3.3. When using the same dosage form and the same dosage form as the standard, the content of the active ingredient in the dosage form shall be expressed as the threshold value, and the dosage shall be expressed as the viscosity per cubic meter (L/hm2): 3.3.5 Other pesticides for controlling electrical hazards If other pesticides are to be used, they should be selected for the test and test objects, and all plots should be treated uniformly, and they should be separated from the test and control drugs to minimize the interference of these drugs. 4.4 Meteorological and environmental materials
4.1.1 Meteorological dataWww.bzxZ.net
Record the almost uniform temperature during the entire test period, expressed in terms of diagnosis. 4.1.2 Soil material
Record soil type, organic matter content, moisture content and other relevant information 4.2 Investigation method, time and frequency
4.2.1 Investigation method
After the seedlings are all grown, randomly dig up the buds in each test plot to check the bud rate. Usually, no less than 25 double-bud seedlings are found in each plot, and each seedling is graded with a small thread to make a vertical line. The grading method is:
Grade 0: No disease
Grade 1: Red lesions appear at both ends of the double-bud seedlings; Grade 2: Red lesions appear at both ends of the double-bud seedlings; Grade 3: Red lesions appear at both ends of the double-bud seedlings; Grade 4: Pathogens only appear at the cut, and the cut accounts for 1% of the seedlings. /37 level: The disease has penetrated into 1/2 of the seedlings:
9 level: The whole seedlings are infected, the internal tissues have turned black, and the seeds are necrotic and cannot be regenerated. 4.2.2 Adjustment time and efficacy
Usually the survey is conducted once, and it must be conducted after all the seedlings are taken care of. 4.2.3 Calculation method of efficacy
Seedling incidence rate () =
Number of diseased seedlings
Total number of seedlings surveyed
Analysis index - the number of diseased seedlings at each level is 2×10 adjusted to the total number of seedlings
Control effect (%) = blank control area disease index (end disease index, control area disease index (incidence rate) × 130~ (3 )The diseased seedlings in the blank control area
4.3 Impact on crops
Observe the process of production of ganergic acid. If there is any activation, the process should be described in detail. In addition, the effects of the drug on crops (promoting maturity, stimulating growth, etc.) should be recorded using the following comparative methods.
) If the drug can be measured or calculated, it should be expressed in absolute values, for example, high (the frequency and intensity of the damage can be expressed in two ways). Show: 1) According to the drug level, the intensity of drug damage in each plot is divided into 1, 10, 10, 10·10, 110·10, 110·10. 1: No drug cellar;
: Slightly no impact on normal growth
10, obvious drug warning, can be explained, will not cause some forms: 10: Commercial drug cellar, slightly impact on normal growth 1 "Drug cellar seriously, cabbage is seriously affected 2) The percentage of the test plot and the blank is compared to evaluate its drug quality: GB/T 17980.101—2004
When recording, the symptoms of drug damage should be accurately described (effects, reports, etc.): provide biofilms, videos, etc. 4.4 Effects on other organisms
4.4.1 Effects on other tobacco pests
Record any effects on other pests and diseases, including beneficial and non-beneficial effects. 4.4.2 Effects on other non-standard organisms
Record the effects of the drug on wild organisms and beneficial insects in the test area. 4. E
Product yield and quality
No records are required.
5 Results
The results obtained in the test should be analyzed by biological statistics methods (especially using EMHT), and the conclusion report should be written in a formal format, and the test station should be analyzed. The original data should be kept for inspection and verification.
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