This standard specifies the determination of sulfadimethoxine and sulfamethoxazole residues in livestock and poultry meat by high performance liquid chromatography. This standard is applicable to the determination of sulfadimethoxine and sulfamethoxazole in livestock and poultry meat. SB/T 10388-2004 Determination of sulfadimethoxine and sulfamethoxazole in livestock and poultry meat SB/T10388-2004 Standard download decompression password: www.bzxz.net

ILS67.12C
Preparation No.: 14169—2004
Domestic Trade Industry Standard of the People's Republic of China SB110388—2004
Determination of sulfamethazine and sulfamelhoxazole in livestock and poultrywwW.bzxz.Net
Deterninalior of gnlfarethazine ad sulfamelhoxazoteiniieat of livestock and poultry2004-08-04Promulgated
Ministry of Commerce of the People's Republic of China
Implementation on 2004-09-01
Standardized by the Ministry of Commerce of the People's Republic of ChinaForeword
SB/T1C3B8—2004
This standard is formulated by the Technical Appraisal Center of the Ministry of Commerce of the People's Republic of China, and the National Institute of Edible Disease Control and Prevention and Hebei Provincial Center for Disease Control and Prevention participated in the formulation and approval of this standard.
This standard has been prepared by Yang Jiying, Ji Zhu, Liu She, Song Shufeng, Han Huixin, Zhang Xinling, Liu Wenma, and the Ministry of Commerce Technical Supervision Center for this standard. Interpretation: 1 Scope
Determination of sulfamethoxazole and sulfamethoxazole in old meat
SB/T 103B8—2094
This standard specifies the determination of the residual amount of sulfamethoxazole, sulfonamide and sulfamethoxazole in separated meat by high performance chromatography, and the determination of sulfamethoxazole and sulfamethoxazole in separated meat by high performance chromatography. 2 Principle
The sulfamethoxazole in the sample is collected by ethyl acetate (10V+1V), and then purified by water distribution. It is detected by high-performance phase chromatography with external detector and quantified by external standard method. 3 Reagents and materials
The following reagents are analytical reagents unless otherwise specified, and water is distilled water. 3.1 Japanese encephalogram (excellent grade, 0.0001mm). 3.2 Density (excellent).
3.3 Hexane.
3.4 ​​Methane:
3.5 Ethylene glycol + trichloromethane (10V1V):
3. 6 Z 1 \ methyl chloride (2V-1V),
3.7 Acidifier
3.8 Sodium sulfate solution, 2% water solution (mass concentration). 3.5.01mol/L salt limit: 3.8g fresh sodium hydrogen sulfide + 1.55g sodium disulfide, freshly mixed with water and adjusted to 1000 rsl, then filtered through a 0.5m pore filter.
3.1C glacial acetic acid,
3.11 Standard concentration 99%. ||t 3.11.1 Sulfonamide: 3.11.2 Sulfonamide. 3.12 Standard solution: Accurately weigh 1U of carbonyl chloride and 2mA of amine, respectively, and add 103mL of each solution. Then add acetonitrile to the solution. The relative concentration is 1Co"/mL. Store in ice and the validity period is 3 months. 12.2 Mix the intermediate solution: Take 5.nmL of each labeled solution in a 50mL volumetric plate and dilute with the mobile phase until the solution becomes sticky. Then you will get a carbon gel and divide it into 1 mL and 1 mL of the standard intermediate solution. Store in the refrigerator and it is valid for 1 month. 3.*2.3 Standard work: Divide the standard intermediate solution into 0.6 mL, 1.25 mL, 3.50 mL and 1.0 mL of each. Dilute to the mark with mobile phase and shake to mix the standard working solution. 0.5.0.10.0, 5.3.50.1.0 g/m1 of standard catalyst series solution, SB/T0386-2CC4
4 Receiver and equipment
4. High performance liquid spectrometer: attached outside the case detector. 4.2 System transfer instrument.
4.3 Disease system product introduction.
4. 4 The sound wave occurs densely
4.5 monitoring software case.
4. Rizhi porous filter module filter data,
5 Australia fixed steps
5.1 sample treatment
sampling is relatively accurate after the test, the whole material is tested and retained in the test, about 1 acid is sold under the shock of V+! Vm model: r editor mn, through the exciting in the natural hair, I enter again 6 mature ten flow 0:12)rl. sound handling nin, 250/min separation: 3ir will not be mixed and evaporated without ten, Ding 12. water resources rotation Transfer to 1 if large, can be divided into several times). 2. Heart nl.3 Huatong Tengji water tank wave rl. Orthodox, point pot with visual homogenizer system set up: light centrifuge tube. 1nnr/min nausea 3, with dense suction to remove the layer, discard. Open again. (Worker health mouth - once, discard the water phase. Add 3m:. Break 1 atmosphere also very suitable for reducing agent (2V! V), ten vortex add Fan about the strong entertainment, with suction to remove the upper layer and have: the lower layer machine transfer to the bottle, at 19. Double calculation to ten. Ocean two people 1.0 flow period solution or whole, full of nutrition on? r:.n, through.1m of the pore energy membrane over the cold, relatively [points, 5.2 determination
5.2.1 quasi-phase chromatography reference
color column Hxr! 2mmX4mm diameter 5m: h) washing phase: alcohol + r..m.: 1. phosphate buffer solution - glacial acetic acid = 2±75-1. 1 detection wavelength: 21m;
minimum) flow rate: 1.UL/m
5.2.2 Standard curve
According to the above conditions, flow through each point of the standard 1 respectively, and determine the final volume of each standard solution at 20, and then calibrate the product with the concentration of the standard solution to calculate the regression coefficient and correlation coefficient. 5.2.23 Detailed determination of the product
Under the above conditions, absorb the purified product E and analyze the new .6 results
6.1 Calculation
The method of aligning the center point and the decline of the benefit must be carried out for regression analysis. The formula (gate) has been designed to calculate the total sample safety and medical, The content of the substance in the vinegar and rubber,
or in:
X-xV:Lm
in the sample must be less than 30 grams of dry matter (mgkg); the content of the substance in the tested liquid must be less than 1 liter (micrograms per liter); the total amount of the tested liquid must be in grams per liter (1 liter); the detection limit is 5B/110388-2G4
. When the amount is 0/1, the amount of the sample must be within the acceptable range of the difference between the two analyses. The average value is about 20%.
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