NY/T 2522-2013 Guidelines for testing specificity, consistency and stability of new plant varieties Persimmon NY/T2522-2013 |tt||Standard compression package decompression password: www.bzxz.net

ICS65.020.20
Agricultural Industry Standard of the People's Republic of China
NY/T2522—2013
Guidelines for the conduct of tests for distinctness, uniformity and stability-Persimmon
(Diospyros kaki Thunb.)
(UPOV: TG/92/4, Guidelines for the conduct of tests for distinctness, uniformity and stability-Persimmon
(Diospyros kaki Thunb.)
(UPOV: TG/92/4, Guidelines for the conduct of tests for distinctness, uniformity and stability Stability-Persimmon, NEQ) 2013-12-13 issued
2014-04-01 implementation
The Ministry of Agriculture of the People's Republic of China issued
Normative reference documents
Terms and definitions
Symbols,
Requirements for propagation materials
Test methods
Specificity, consistency and determination of stability results Characteristic table
Explanation of the character table
Grouped characters…
Technical questionnaire
Persimmon character table
Appendix A (Normative Appendix)
Appendix B (Normative Appendix)
Appendix C (Normative Appendix)
Explanation of the character table of persimmon
Format of technical questionnaire on persimmon.
NY/T 2522—2013
NY/T 2522—2013
This standard was drafted in accordance with the rules given in GB/T 1.1-2009. Introduction
This standard was revised using the redrafting method and adopted the International Union for the Protection of New Varieties of Plants (UPOV) Guidelines "TG/92/≤, Guidelines far the eonduct of tests for distinctness, uniformity and stabiliry-Persimmon\This standard corresponds to UPOV Guide TG/92
The consistency between the standard and TG
is non-equivalent.
This standard has technical differences compared with UPOV Guide TG/4, the main differences are as follows: 8 traits were added:
Flower: Flower shape"
Deleted 7 traits
Shape" "Persimmon pedicle
Adjusted"
"Astringent persimmon fruit
Color" was revised
This standard is
This standard is
This standard is
This standard exceeds
The main items of this standard are: "length of bud tip", "petal color of female flower", "female leaf blade, fruit variety type"; "degree of cracking", "cross-section shape of fruit", "sweet persimmon peel color" and "fruit peel color", "concave degree of astringent persimmon flesh", and "pedicle concave".
SEARCH CENTER
The Beijing Seed Administration Bureau proposed
Technical Committee for Standardization of Testing Methods for New Plant Varieties
Beijing Agriculture and Forestry Science and Technology University
Gong Renku Chen Qicun Pei Xiaofeng Yang Hui
Yang Zai Li Tan Ge
Wang Jianping, Du Lianmeng.
1 Scope
NY/T2522—2013
Guidelines for Testing Specificity, Consistency and Stability of New Plant Varieties Persimmon
This standard specifies the technical requirements for testing specificity, consistency and stability of new persimmon varieties and the general principles for judging the results. This standard applies to the testing of new varieties of persimmon (Dio. spyroskaki Thunb.). 2 Normative References
The following documents are indispensable for the application of this document. For all referenced documents with dates, only the versions with the dates are applicable to this document. For any undated referenced document, its latest version (including all amendments) shall apply to this document. GB/T 19557.1 Guidelines for specificity, consistency and stability testing of new plant varieties General Principles 3 Terms and definitions
The terms and definitions defined in GB/T 19557.-1 and the following terms and definitions apply to this document. 3.1
Individual measurement.- mneasorement of a nurnber of individual plants or parts of plants. A group of individual records are obtained by measuring a certain organ or part of a batch of plants. 3.2
Group visual inspection: tissment by a single chservation of a group of plants or parts of plants. A group record is obtained by visually inspecting a certain organ or part of a batch of plants. 4 Symbols
The following symbols apply to this document,
MS: individual measurement
VG: population measurement
QL: quality trait,
QN: quantitative trait,
PQ: pseudo-quality trait.
*: The trait marked as P()V is used for the important traits required for variety description. Unless the expression state of the trait cannot be tested due to environmental conditions, all P()V members should use these traits. (a)-~(d): The marked content is explained in detail in B.2 (i); The marked content is explained in detail in B.3: The underline in this document is a special reminder of the scope of application of the test trait. 5 Requirements for propagation materials
5.1 Propagation materials are provided in the form of seedlings or scions. 5.2 The number of seedlings or scions submitted shall be at least 10 plants (branches). 5.3 The submitted propagation materials should be healthy in appearance, high in vitality, and free from pests and diseases. Seedlings should be one-year-old grafted seedlings, and the species of brick and wood should be marked. Grafting NY/T 2522—2013
Secrets should be healthy dormant vegetative scions, and each scion should have at least IC effective buds. 5.4 The propagation materials submitted are generally not subjected to any treatment that affects the expression of variety traits. If they have been treated, detailed descriptions of the treatment should be provided.
5.5 The propagation materials submitted should comply with the relevant provisions of China's plant quarantine. 6 Test methods
6.1 Test cycle
The test cycle is at least 2 independent annual growth cycles. Normal fruits should be produced before the end of each growth cycle. A complete growth cycle of persimmon refers to the entire growing season from the beginning of budding (flower buds and/or vegetative buds), through flowering, fruit ripening, entering dormancy, and until the end of the dormancy period (new buds swelling). 6.2 Test location
The test is usually carried out at one location. If some traits cannot be fully expressed at this location, they can be observed at other qualified locations.
6.3 Field test
6.3.1 Test design
Please plant the varieties and similar varieties adjacently. Seedlings are directly planted: single plant plot, 5 times of replication, row spacing 5m, plant spacing 2m. The scion is grafted onto the perennial cow's junqianzi (Dias yrosiotus L.) or other suitable rootstocks by branch grafting. 6.3.2 Field management
Carry out the normal production management method of the persimmon orchard. 6.4 Character observation
6. 4. 1 Observation period
Character observation should be carried out according to the growth and development stages listed in Appendix A. Appendix B explains these growth and development stages. 6.4.2 Observation methods
Observation of traits should be carried out in accordance with the observation methods (VG, MS) specified in Appendix A. 6.4.3 Number of observations
Unless otherwise specified, the number of samples for individual observation traits (MS) shall not be less than 5. When observing the organs or parts of the plants, the number of samples for each plant shall be 2. The entire plot shall be measured for group observation traits (VG). 6.5 Additional tests
If necessary, additional tests may be performed on traits not listed in Appendix A. 7 Determination of specificity, consistency and stability results 7.1 General principles
Specificity, consistency and stability shall be determined in accordance with the principles determined in Membrane CB/Work 157.1. 7.2 Determination of specificity
The variety under application should be clearly distinguishable from all known varieties. In the test, when the application variety has a significant and reproducible difference with similar varieties in at least one trait, the application variety can be determined to have specificity. 7.3 Determination of consistency
Use a 1% population standard and at least 95% acceptance probability. When the sample size is 5 plants, no heteromorphic plants are allowed. 7.4 Determination of stability
If a variety has consistency, it can be considered stable. Generally, stability is not tested. 2
NY/T 2522—2013
If necessary, the seedlings or scions of the variety can be replanted or grafted. Compared with the previously provided propagation materials, if there is no significant change in the expression of the trait, the variety can be determined to have stability. 8 Trait table
According to the test needs, the traits are divided into basic traits and test traits. Basic traits are traits that must be used in the test. Appendix A lists the basic traits of persimmon.
8.1 Overview
The trait table lists the trait name, expression type, expression state and corresponding code and standard variety, observation period and method, etc. 8.2 Expression type
According to the expression method of the trait, the traits are divided into three types: quality traits, pseudo-quality traits and quantitative traits. 8.3 Expression state and corresponding code
8.3.1 Each trait is divided into a series of expression states. In order to facilitate the definition of traits and standardized descriptions, each expression state is assigned a corresponding digital code to facilitate data recording, processing and the establishment and communication of variety descriptions. 8.3.2 For quality traits and pseudo-quality traits, all expression states should be listed in the test guide. For quantitative traits, in order to shorten the length of the trait table, the expression states of even codes can be omitted. The expression states of even codes can be described in the form of the previous expression state to the next expression state.
8.4 Standard varieties
The standard varieties corresponding to the expression states of some traits are listed in the trait table to help determine the different expression states of related traits and correct the differences caused by years and locations.
9 Explanation of trait table
Appendix B provides additional explanations on the observation period and observation methods of some traits in the trait table. 10 Grouped traits
In this document, the cultivar grouping traits are as follows: a) *Fruit: Fruit size (Table A.1 Neutral trait 26). b) *Fruit: Fruit shape (Table A.1 Neutral trait 27). c) *Fruit: Peel color (Table A.1 Neutral trait 28). d) *Sweet persimmon, fruit: Fruit edible maturity period (Table A.1 Neutral trait 45). e) *Astringent persimmon: Fruit: Fruit edible maturity period (Table A.1 Neutral trait 16). Fruit: Variety type (Table A.1 Neutral trait 47). f
11 Technical Questionnaire
The applicant should fill in the persimmon technical questionnaire in the format given in Appendix C. -
NY/T2522—2013
Basic characteristics of persimmon are shown in Table A. 1.
·Tree body
*Branch: length
Branch: internode length
Branch: lenticel size
Branch: anti-reed shape
Branch: lenticel density
Branch: bud tip exposure
*Branch: bud end surface shape
Branch: color (sunny side)
Appendix Record A
(Normative Appendix]
Persimmon Characters Table
Persimmon Basic Characters Table
Observation Period and Method
Expression State
Half-open
Oval
Narrow Shape
Triangle
Short Round
OblongbzxZ.net
Yellow Color
Gray Brown
Reddish Brown Color
Standard variety
Chicken heart yellow
Xinpan persimmon
Haian small square persimmon
Xicun early-born
Flat core dead
Fuping sharp
Meixian ox heart persimmon
Mopan persimmon
Meili ox heart persimmon
July red
Flat core free
Queen persimmon
Meixian ox heart persimmon||t t||Mopan Persimmon
Huanping Sharp Persimmon
Mopan Persimmon
Meixian Niuxin Persimmon
Fuping Sharp Persimmon
Pinghe Wu
July Red
Bud Cutting Period
Leaf: Length
Leaf: Width
Table AI (Continued)
Observation Period and Method
Expression Status
NY/T 2522—2013
Standard variety
None flat core
Yuhuan long
None flat core
Meixian Niuxinchi
Mopan persimmon
Huixian small persimmon
UBESHING
*Leaf shape
STANDARD
Leaf: top shape
*Leaf
（t）
Female flower: open
?Plant: Sex type| |tt||★Male flower: crown diameter
Flower: petal color
Carving: equal piece shape makeup
dark green
yellow green
even with flowers
muffled white
regular cross shape
irregular cross shape
Lantian water persimmon
SRESEARCHEEN
five flower persimmon
fire crystal persimmon
flat coreless
Lintong green
NY/T 2522-—2C13
*Carved flowers: Number of flower scales
Female flowers: Shape of flower tube
Net fruit, nest fruit size
Table A1 (continued)
Observation period and method
Pei Da state
Greater than 4
Xi Ling shape
High four-angle
Short oval
Standard variety
Fuping Jianpei
Huping persimmon
Oval
PUBHISHING
STANDAR
*Fruit: Fruit shape
Fruit single!
Fruit: Fruit base truth
Fruit: Fruit base scar type
Fruit: Fruit top cross groove
Fruit: Fruit top shape (longitudinal section)
Rectangular
Fan-shaped shadow
Young vein color
Orange-red
Partially available
Through prototype
Ring type
Inner cover type
None or not obvious
Boai August Yellow
ARCH GENG
Chengjiang Niuxinlang
Yugulan
Xiangyang Niuxinzhong
Tianbaogai
Mopanshi
Mopanshi
Zhouboshi
Small paste
Fruit: stalk length
Fruit: stalk thickness
Fruit: pedicel shape
Table A' (continued)
Observation period and method
Expression status
VY/T 2522—2013
Standard variety
Meixian Niuxin Persimmon
Yangshuo Niuxin Persimmon
PUBHSHING
STANDARD
Fruit: cotton stalk, concave and convex
Wu Shi.Diao
*Many fruits
?Fruit, soft and loose flesh color
Fruit: flesh brown spots
Fruit: flesh brown spots size
·Sweet Drag
Fruit: Edible Fruit Maturity Period
Fang Yinxing
Sigou Persimmon
Xian Niuxin
Plant Red
Gaoping Jianwei
Some Have
Chendengwan
Black Heart Persimmon
Gan Baizi
Xiongsiwan
Xihansheng
Sweet Baogai
NY/T 2522—2013
*According to the following:
Fruit: Fruit edibility Blackening period
Fruit: Variety type
Table A.1 (continued)
Observation period and method
Seed size
STANDARD
Seed shape
Seed: Brown plug degree
Expression status
Completely astringent
Completely sugar
Incompletely astringent cotton
Incomplete product box
Standard variety
July grass
Fuping sharp Nan
Fuping sharp persimmon
Pingheguang
Chendengwan
Fire product cabinet
Fuping sharp persimmon
B.1 Persimmon growth stage
See Table B. 1.
Dormancy period
Bud burst period
Leaf formation period
Fruit hardening period
After-ripening and education period
Explanation of multiple traits
Appendix B
[Normative Appendix]
Explanation of persimmon trait table
Table B.1 Persimmon growth stage table
The period after the tree leaves fall and before the beginning of bud formation
The period when the buds on the branches begin to show golden yellow bud tipsOn persimmon trees: The period from 25% to 75% of the flowers are openThe period from the time when the flowers are formed into 1 leaf to the time before the leaves fallThe period when the fruit reaches its inherent color and the seed coat has dried upThe period when the fruit is ripened naturally or artificiallyThe period when the fruit is ripened more than 10 cm in lengthThe period when the dormancy period is observed. (h)
Observe the middle part of the branches in summer and autumn.
Observe the flowers in the middle nodes of the branches during the flowering period. (d)
Observe the fruit ripening period.
Explanation of individual traits
See Table A, 1 for trait classification and codes in the figure,
NY/T 2522-2013
Trait 1*Tree posture, see Figure B. 1. Observe the outline of the entire tree, and also observe according to the growth angle of the lower main branches. Upright
Trait 2*Branch: length, see Table B.2.
Half a pound
Tree: posture
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