Guidelines for the conduct of tests for distinctness,uniformity and stability Durum wheat
Some standard content:
1CS 65.020.20
National Standard of the People's Republic of China
GB/T19557.3——2004
Guidelines for the conduct of tests for distinctncss, uniforinity and stability of new plant varieties
Durum wheat
Guidelines for the conduct of tests for distinctncss, uniforinity and stability of new plant varieties stability-Durumwhcat (TriticumdurumDest) Issued on June 11, 2004
General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China Administration of Standardization of the People's Republic of China
Implementation on December 1, 2004
GB/T19557.3—2004
Normative references
Technical explanation and definitions
Requirements for test tolerant varieties
Observation and identification of traits
7 Traits
Appendix A (auxiliary appendix) Table of test traits of hard-grained small-grain wheat varieties (Excludes Appendix B (Normative Appendix)
Explanation of traits
Appendix C (Existing Normative Appendix)
Technical method for testing new varieties of hard-grained wheat 10
Appendices A, B and C of this standard are normative appendices. This standard was proposed by the Ministry of Agriculture of the People's Republic of China. The drafting units of this standard are the Science and Technology Development Center of the Ministry of Agriculture and the Sichuan Academy of Agricultural Sciences. The main contributors to this standard are: Zhu Rubang, Yang Wuyun, Yu Xiao, Hou Bo, Chen Ruming, Na Wanhua, Pei Jianfan. GB/T19557.3—2004
1 Scope
Guidelines for testing the specificity, consistency and stability of new plant varieties Durum wheat
GB/T19557.3—2004
This standard defines the technical requirements for testing the specificity, consistency and stability of new varieties of hard grains, and the judgment of the test results is in the format of point technical reports.
This standard applies to the testing and evaluation of the specificity, consistency and qualitative properties of hard grains (IriticunurumLesl). 2 Normative references
The following documents have been incorporated into the clauses of this standard through reference in this document. Except for the date of the reference, the subsequent revisions (excluding errors) do not apply to this standard. However, the latest versions of these documents may be used after the parties have reached an agreement based on this standard. For references without specifying the date, the latest version shall apply to this standard. GR/3E43 Inspection of crop species
GB135i Wheat
B44J=.1 Inspection of food crop seeds Cereals
GB/T5=11 Inspection of protein quality of single food and oilseeds GB/T19557.1:2004 Guidelines for testing specificity, consistency and qualitative characteristics of new species of prey General Principles 3 Terms and definitions
The terms and definitions specified in GB/T19557.1:2004 apply to this standard. 4 Requirements for seeds of test varieties
4.1 Quality and quantity of seeds of test varieties
4.1.1 The quality of hard-grained small seeds for reverse test shall at least meet the requirements of original seeds in G4401.:, the quantity is 1k, 4.1.2 If the hard-grained small new varieties (including new combinations, new germplasm and new materials) submitted have special uses, the quality and quantity of the submitted seeds can be determined according to their characteristics and application areas, depending on the specific circumstances. For selective test items, the quality and quantity requirements of the submitted seeds are shown in Table 1.
Table 1 Requirements for submission of selective test items Seed sensitivity and quality items
Disease resistance
Environmental resistance
4.2 Treatment requirements for test variety seeds
Seed quantity
Seed approval authority shall agree with you that the submitted seeds shall not be subjected to any treatment that affects the growth of the seed plants. If treatment is carried out, a detailed explanation of the treatment shall be given.
4.3 Storage of test variety seeds
After receiving the test seeds, the testing unit shall immediately separate the stored seeds and bring them with it for inspection. 4.4 In addition to submitting the seeds, the tester shall also fill in the "Technical Document" according to GB/T19557.3-200d4
5 Test
5.1 Test time| |tt||The test time should be at least two alternate seasons or about the growth cycle. 5.2 The test site should be able to ensure the normal growth and expression of the test variety and its characteristics. One site should be set for each test variety. If there are special requirements, multiple sites can be tested. 5.3 Set up
67m2 plots in the field. Each plot has 110,000 to 130,000 seedlings. The row spacing is 70cm×3m when sowing in holes. When sowing in rows, the row spacing is 23cm. Each test variety has at least two blocks. The environmental conditions between blocks should be consistent, and separate rows and protection rows should be set.
5.4 The management of the test site is basically the same as that of the field. The field management of the test variety and the test variety should be strictly consistent. 5.5 Sampling and Observation
The approval authority shall determine the test avoidance characteristics of the variety based on the strong data provided by the applicant. The sampling, secret and brushing of the sample shall be carried out in accordance with the requirements of the corresponding characteristics in Appendix B. The observation samples shall come from the middle of the plot or the area without environmental and human factors. The observation data area shall be taken from two plot groups.
5.6 Other
The setting of test pieces and test points for the selective sampling test items shall meet the requirements of Appendix B. When there are special requirements for the approved variety, additional tests can be carried out.
The seeds used for the second test of the same growth season are the seeds harvested in the first growing season. Sterile lines and hybrids are seeds prepared in the previous year.
6 Observation and judgment of traits
6.1 Basic requirements for observation and judgment
6.1.1 The observation of traits shall be carried out in accordance with the requirements of Appendix A and Appendix B. If the secondary test traits of the standard variety are not expressed or judged due to force majeure or human factors, the test shall be repeated. 6.1.2 The record of observation shall be carried out in accordance with the requirements of GB/T195.7.1-2004, and the final calculation results shall be converted into codes and reported, and the recorder shall sign. The original records shall be reviewed and signed by the reviewer and auditor. 6.1.3 Judgment of specificity, consistency and stability shall be carried out in accordance with the requirements of GB/T19557.1-2004. 6.2 Judgment of specificity
If the same trait of the test variety and the similar variety is the same code, it means that the test variety has no difference with the similar variety in this trait. If the test variety has one quality trait or two or more quantity traits that are different from the near variety, or one quantitative trait has two or more codes different from the near variety, it can be determined that the test variety is different from the near variety. 6.3 Consistency judgment: The test variety is analyzed by taking the code as the analysis unit, and the mutation rate is calculated. If the mutation rate of the whole plot is lower than 2.5% and the mutation rate of the test variety does not exceed the mutation rate of the near variety in this trait, the test variety will be regarded as having consistency. Traits that cannot be tested individually cannot be identified for consistency. 6.4 Consistency judgment: If the same trait of the test variety is tested in the same generation in two identical growing seasons, or the mutation rate of the second test is not significantly different from that of the first test, it means that the variety is stable in this trait, otherwise it is unstable. 7 Characters
CI/T 19557.3—2004
The test of grains includes 0 mandatory characters and supplementary characters. The mandatory characters are the basic characters of the test and examination plan for each variety. The supplementary characters are the characters selected for further testing when the mandatory characters cannot distinguish the variety from similar varieties. 7. 2 Test characters (22 characters)
Color of the anthocyanin, yellowish color, budding time, wax powder of flag leaf sheath, powder on the back of flag leaf blade, wax powder of the neck, powder on the neck, plant height, shape of reed, relationship between the length of the small sugar awn and the length of the awn. Forehead shape, length of the tip, shape of whiskers, outer surface of the glume, thickness of the cross section, color, color, shape of iodine, shape of grain, grain carbolic acid staining, winter swallowing, dry grain weight. 7.3 Supplementary traits (18 items)
Anthocyanin color of the leaf, plant growth rate of the leaf, color staining of the Miscanthus flower, pubescence of the individual nodes, shape of the vine, shoulder width, pubescence of the stalk, facet shape. Stem color, pubescence of the grain tail, lodging resistance, coarse convex content, resistance to streaking disease, resistance to powdery mildew, resistance to succumb, padding, compatibility, and production, GR/T19557, 3-2004
1.2. The color of the first leaf flower is bright
5, activity
5. Seeding period, (the first sample can see the first
shallot
6 hours morning
. Wax
8. Seven flowers are blue and white
(Normative Appendix)
Hard grain small plastic variety test trait decline
Table A. 1 Trait table
Sinking period
c9--11
35 ~t9
Trait is close
Half sound collection
Standard products and
Lin 16
Alrar8.Snzgi4
I.xhn/3/Gde:v2469/An//T:
Shag22
Altars
Mexieali7s
Risp,Deakioa
Du:ex, Duifor:
Aldene. Dules
Sle, Alt ayey
Swew3. Raron?
Aldente
Aidarxits
L.sha/1/Gcnv2463/Ap//Tg
Srigna
?, lower section
10, Ge Pin wax pad
11. Type structure
1E. Not (opening to reason
six containing Yun)
15. Miscanthus type-|| tt||:4. The small mango is long and the god is long
Guan Ru·
15. Protect the shape of Huizhong 1/a at the center
16. More self-seeking: <inter-characteristics 15
17. The axis has the same character 15
Observation period
55·75
SC--Gc
75--92
TE-~92
9:-~- 92
Table A.1《Continuation》
Character donation
Zhi Yi Shen Chang
Characteristics and appearance*
Elongated
High frequency
Narrow
GB/T19557.3—2004
Soft standard varieties
Fuinc:
Alhnr&1, Ris22
Erolig
Sanzhuanwu Tanlan
Aitar34
Etai Bangbanglan Seat
Be-rig5
Sle, Mexicalir5
Altar64
3ettius
GD/T 19557.3—20Q4
18. Exact length~ (same character 15)
10. Pre-piercing shape: (concentric shape 15)
20, chlorine outside forget ring hair
21. Open cut surface early high
22. Silver colorbzxz.net
21. Double color (period)
25. Seed with surface shape
9(1-~93
9(1r95
Table A.1 (continued)
Descending fingering
Point one bend
Medium central classic
Rectangular
Near fall fast
Medicine Xiangge
Standard home species
Chen./Al-a :84/:30=hz
Selga-5
Lel:u/3/Gdovx+69/Aa//Pg
Altaro4
Aldente.Cocrit
Shagl:.Raecorl
Esulia.Sp.gas
Rinua,Aruba
A:1erg:
Sl:sg22
Altu81
27，Grain shape
.Tennibu velvet
29，Grains are chrysanthemum-like in color, color-developing
30% winter nature
3]. Blue color【hot milk】: Anti-inflammatory properties 33. Dry granules 1. Granular protein content Detection period 10--33 Table 1 (continued) Properties Description Shape Long-lasting Extremely long-lasting Medium-to-long-lasting GA/T 19557.3-2004
Standard variety
them/A(rer84//yoe.18
Slheg22
Sheg14
Sheg14
(B/T19557.3—2004
35, resistance to blast disease (my period
36, resistance to powdery mildew (bulb period)
37, resistance to equal control
3%, salt resistance
39. Resistance to only party period
40, resistance
, "must be tested traits
Table A. 1 (Continued)
Character description
Standard species
Not damaged||Table 4.2 Grain change test period decimal code table
First leaf in semi-shaded
No new growth
First leaf from bud
Starting from the beginning! Expand
Fifth expansion
Ninth or more leaves open
Only the upper part
First leaf can appear
Five leaves appear
Appear with the first or second leaf
The first leaf appears together
Flag leaf Tongue/mountain ring
1:2~63
1:1--$5
1:5~1:9
F4~·F5
6R~·F9
Big period
Thia leaf period
The first seven roots
The first: small flower point
The small mountain appears
1/2 of the small dragon comes out
34 of the small is
The small flower is completely out
And the flowering period
Half of the small flower slices
Sweat flower special bundle
Planting-sinking mature shot
I am happy to be mature
GB/E 19557.3—2004
Mid-term
Early stage of marriage
Middle stage of marriage
Maturity
Fruit (use fingernails to distinguish!
To prevent seed from coming out)
Overripe, fresh, steamed, period
Lightly sleep
Vital God 0%
God ends with body mouse
! Reading guide secondary rest reflection
Second body reflection conclusionMore self-seeking: <inter-character 15
17. Axis has the same character 15
Observation period
55·75
SC--Gc
75--92
TE-~92
9:-~- 92
Table A.1 "Continuation"
Character donation
Knowing one god long
Geometry appearance*
Long Ou shape
High frequency activity
Get narrow
GB/T19557.3—2004
Soft standard variety
Fuinc:
Alhnr&1, Ris22
Erolig
Sanzhuanwu Tanlan
Aitar34
Etai Bangbanglan Seat
Be-rig5
Sle, Mexicalir5
Altar64
3ettius
GD/T 19557.3—20Q4
18. Exact length~ (same character 15)
10. Pre-piercing shape: (concentric shape 15)
20, chlorine outer ring hair
21. Opening surface early high
22. Amber color
21. Two-color (period)
25. Seed surface system
9(1-~93||t t||9(1r95
Table A.1 (continued)
Falling fingering
Point a bend
Medium Central Classic
Rectangular
Near throw fast
Medicine Xiangge
Standard home species
Chen./Al-a:84/:30=hz
Selga-5
Lel:u/3/Gdovx+69/Aa//Pg
Altaro4
Aldente.Cocrit
Shagl:.Raecorl
Esulia.Sp.gas
Rinua,Aruba
A:1erg:
Sl:sg22
Alt u81
27, grain shape
. Tunisian velvet
29, grain color is chrysanthemum color, color
30% winter color
3]. Blue grain color [heat milk
: anti-example
33. Dry grain
1. Grain protein content
Test period
10--33
Table ^, 1 (continued)
Characteristics
Heat shape
Elongation
Extreme injection
Medium type
GA/T 19557.3-2004
Standard variety
them/A(rer84//yoe.18
Slheg22
Sheg14
Sheg14
(B/T19557.3—2004
35, resistance to blast disease (my period
36, resistance to powdery mildew (bulb period)
37, resistance to equal control
3%, salt resistance
39. Resistance to only party period
40, resistance
, "must be tested traits
Table A. 1 (Continued)
Description of properties
Standard species
Not spoiled T species in
Decay Table 4.2 Small grain changes in the current measurement period decimal code table
The first leaf is semi-sticky and shade-grown
Lack of new growth
The first leaf starts from the bud
! Expand
Fifth expansion
Ninth or more leaves open
Only the upper part
The first part can appear
The fifth part appears
Appears with the first or the second part
The first person's view
Flag leaf appears together
Flag leaf tongue/ring appearance
1:2~63
1:1--$5
1:5~1:9
F4~·F5| |tt||6R~·F9
The period is large
The period of thiocyanate is large
The seventh root
The first: small flower point
The hill appears
1/2 of the small dragon comes out
34 is
The small flower is completely out
And the flowering period
Half of the small flower slices
Sweat flower special bundle
Planting-sinking shot
I am happy with the water
GB/E 19557.3—2004
Mid-term
Early stage of marriage
Middle stage of marriage
Maturity
Fruit (use fingernails to distinguish!
To prevent seed from coming out)
Overripe, fresh, steamed, period
Lightly sleep
Vital God 0%
God ends with body mouse
! Reading guide secondary rest reflection
Second body reflection conclusionMore self-seeking: <inter-character 15
17. Axis has the same character 15
Observation period
55·75
SC--Gc
75--92
TE-~92
9:-~- 92
Table A.1 "Continuation"
Character donation
Knowing one god long
Geometry appearance*
Long Ou shape
High frequency activity
Get narrow
GB/T19557.3—2004
Soft standard variety
Fuinc:
Alhnr&1, Ris22
Erolig
Sanzhuanwu Tanlan
Aitar34
Etai Bangbanglan Seat
Be-rig5
Sle, Mexicalir5
Altar64
3ettius
GD/T 19557.3—20Q4
18. Exact length~ (same character 15)
10. Pre-piercing shape: (concentric shape 15)
20, chlorine outer ring hair
21. Opening surface early high
22. Amber color
21. Two-color (period)
25. Seed surface system
9(1-~93||t t||9(1r95
Table A.1 (continued)
Falling fingering
Point a bend
Medium Central Classic
Rectangular
Near throw fast
Medicine Xiangge
Standard home species
Chen./Al-a:84/:30=hz
Selga-5
Lel:u/3/Gdovx+69/Aa//Pg
Altaro4
Aldente.Cocrit
Shagl:.Raecorl
Esulia.Sp.gas
Rinua,Aruba
A:1erg:
Sl:sg22
Alt u81
27, grain shape
. Tunisian velvet
29, grain color is chrysanthemum color, color
30% winter color
3]. Blue grain color [heat milk
: anti-example
33. Dry grain
1. Grain protein content
Test period
10--33
Table ^, 1 (continued)
Characteristics
Heat shape
Elongation
Extreme injection
Medium type
GA/T 19557.3-2004
Standard variety
them/A(rer84//yoe.18
Slheg22
Sheg14
Sheg14
(B/T19557.3—2004
35, resistance to blast disease (my period
36, resistance to powdery mildew (bulb period)
37, resistance to equal control
3%, salt resistance
39. Resistance to only party period
40, resistance
, "must be tested traits
Table A. 1 (Continued)
Description of properties
Standard species
Not spoiled T species in
Decay Table 4.2 Small grain changes in the current measurement period decimal code table
The first leaf is semi-sticky and shade-grown
Lack of new growth
The first leaf starts from the bud
! Expand
Fifth expansion
Ninth or more leaves open
Only the upper part
The first part can appear
The fifth part appears
Appears with the first or the second part
The first person's view
Flag leaf appears together
Flag leaf tongue/ring appearance
1:2~63
1:1--$5
1:5~1:9
F4~·F5| |tt||6R~·F9
The period is large
The period of thiocyanate is large
The seventh root
The first: small flower point
The hill appears
1/2 of the small dragon comes out
34 is
The small flower is completely out
And the flowering period
Half of the small flower slices
Sweat flower special bundle
Planting-sinking shot
I am happy with the water
GB/E 19557.3—2004
Mid-term
Early stage of marriage
Middle stage of marriage
Maturity
Fruit (use fingernails to distinguish!
To prevent seed from coming out)
Overripe, fresh, steamed, period
Lightly sleep
Vital God 0%
God ends with body mouse
! Reading guide secondary rest reflection
Second body reflection conclusion1 (continued)
Description of properties
Standard species
Not spoiled T species in
Decay Table 4.2 Small grain variation Commercial measurement period decimal code table
First leaf is semi-sticky and shade-cultivated
Lack of new growth
The first leaf starts from the bud
! Expand
Fifth expansion
Ninth or more leaves open
Only the upper part
The first part can appear
The fifth part appears
Appears with the first or the second part
The first person's view
Flag leaf appears together
Flag leaf tongue/ring appearance
1:2~63
1:1--$5
1:5~1:9
F4~·F5| |tt||6R~·F9
The period is large
The period of thiocyanate is large
The seventh root
The first: small flower point
The hill appears
1/2 of the small dragon comes out
34 is
The small flower is completely out
And the flowering period
Half of the small flower slices
Sweat flower special bundle
Planting-sinking shot
I am happy with the water
GB/E 19557.3—2004
Mid-term
Early stage of marriage
Middle stage of marriage
Maturity
Fruit (use fingernails to distinguish!
To prevent seed from coming out)
Overripe, fresh, steamed, period
Lightly sleep
Vital God 0%
God ends with body mouse
! Reading guide secondary rest reflection
Second body reflection conclusion1 (continued)
Description of properties
Standard species
Not spoiled T species in
Decay Table 4.2 Small grain variation Commercial measurement period decimal code table
First leaf is semi-sticky and shade-cultivated
Lack of new growth
The first leaf starts from the bud
! Expand
Fifth expansion
Ninth or more leaves open
Only the upper part
The first part can appear
The fifth part appears
Appears with the first or the second part
The first person's view
Flag leaf appears together
Flag leaf tongue/ring appearance
1:2~63
1:1--$5
1:5~1:9
F4~·F5| |tt||6R~·F9
The period is large
The period of thiocyanate is large
The seventh root
The first: small flower point
The hill appears
1/2 of the small dragon comes out
34 is
The small flower is completely out
And the flowering period
Half of the small flower slices
Sweat flower special bundle
Planting-sinking shot
I am happy with the water
GB/E 19557.3—2004
Mid-term
Early stage of marriage
Middle stage of marriage
Maturity
Fruit (use fingernails to distinguish!
To prevent seed from coming out)
Overripe, fresh, steamed, period
Lightly sleep
Vital God 0%
God ends with body mouse
! Reading guide secondary rest reflection
Second body reflection conclusion
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