This standard specifies the methods and basic requirements for field efficacy plot tests of insecticides for controlling corn borer (Ostriniaf urnacalis, O. nu bilalis). This standard applies to field efficacy plot tests and efficacy evaluation of insecticides for controlling corn borer larvae for registration. Other field efficacy tests shall refer to this standard. GB/T 17980.6-2000 Guidelines for field efficacy tests of pesticides (I) Insecticides for controlling corn borer GB/T17980.6-2000 Standard download decompression password: www.bzxz.net
This standard specifies the methods and basic requirements for field efficacy plot tests of insecticides for controlling corn borer (Ostriniaf urnacalis, O. nu bilalis). This standard applies to field efficacy plot tests and efficacy evaluation of insecticides for controlling corn borer larvae for registration. Other field efficacy tests shall refer to this standard.

GB/T17980.6—-2000
Field efficacy test is one of the important contents of pesticide registration management in my country, and is an important technical basis for the formulation of pesticide product labels, while labels are the only guide for the safe and rational use of pesticides. In order to standardize the methods and contents of pesticide field tests, make the tests more scientific and unified, and align with international standards, so that my country's efficacy test reports have international recognition, the national standards for my country's field efficacy test guidelines are specially formulated. This series of standards refers to the field efficacy test guidelines of the European and Mediterranean Plant Protection Organization (EPPO) and similar guidelines of the Food and Agriculture Organization of the United Nations (FAO) in the Asia-Pacific region. It is formulated based on my country's actual situation and verified by a large number of field efficacy tests. Corn is one of the important corn pests in my country, and pesticides are often used for prevention and control in production. In order to determine the optimal field dosage for corn prevention and control, test the effects of pesticides on crops and non-target beneficial organisms, and provide a basis for the efficacy evaluation and safe and rational use technology of pesticide registration, this standard is specially formulated.
This standard is one of the standards in the Guidelines for Field Efficacy Trials of Pesticides () series, but it is an independent standard in itself. This standard is proposed and managed by the Ministry of Agriculture of the People's Republic of China. This standard is drafted and interpreted by the Pesticide Control Institute of the Ministry of Agriculture. The main drafters of this standard are Chen Jingfen, Jiang Hui, Wang Xiaojun, Ka Yan, Wu Shixiong and Wang Shaocheng. 365
1 Scope
National Standard of the People's Republic of China
Guidelines for the field efficacy trials (I)
Pesticide-
Guidelines for the field efficacy trials (I)Insecticides against corn borerwormGB/T 17980.6—2000
This standard specifies the methods and basic requirements for field efficacy trials of insecticides against corn (Ostrinia furnacalis, O.nubilalis).
This standard applies to the field efficacy plot test and efficacy evaluation of insecticides for registration to control corn larvae. Other field efficacy tests shall be carried out in accordance with this standard.
2 Test conditions
2.1 Selection of test objects, crops and varieties The test objects are corn toad larvae.
Any variety of test crops can be selected. Record the variety name. 2.2 Environmental conditions
Field tests should be carried out in large areas of corn fields and plots where pests occur all year round and are more serious. The cultivation conditions (soil type, fertilizer, tillage, row spacing, water depth) of all test plots must be uniform and consistent and in accordance with local scientific agricultural practices (GAP. 3 Experimental design and arrangement
3.1 Pesticides
3.1.1 Experimental Pesticides
Indicate the trade name/code, Chinese name, generic name, dosage form content and manufacturer of the pesticide. The experimental pesticide treatment shall be no less than three doses or the dosage specified in the agreement (the experimental agreement signed by the test entrusting party and the test undertaking party). 3.1.2 Control Pesticides
The control pesticide must be a registered product that has been proven to have good efficacy in practice. The type and mode of action of the control pesticide should be similar to that of the experimental pesticide and the local commonly used dosage should be used. Special circumstances may be determined according to the experimental pesticide. The purpose of the test depends on the plot arrangement. 3.2 Plot arrangement
3.2.1 Plot arrangement
The plot treatments of the test agent, control agent and blank control are generally arranged in random blocks. Special circumstances must be explained. 3.2.2 Plot area and repetition
Plot area: 50m2. Select 6 rows of corn, with 2 rows on the edge as protection rows, and the remaining 4 rows should be 10m long each. There should be at least 160 seedlings in the plot.
Number of repetitions: at least 4 repetitions.
3.3 Application method
3.3.1 Usage
GB/T 17980.6--2000
Perform according to the requirements of the agreement and the instructions on the label. The application of pesticides should be adapted to local scientific agricultural practices. 3.3.2 Use of equipment
Select commonly used equipment in production, and record all information on the type of equipment used and operating conditions (operating pressure, nozzle diameter). The application of pesticides should ensure accurate dosage and uniform distribution. The deviation of the dosage should be recorded if it exceeds ±10%. 3.3.3 Application time and frequency
Spring corn is used to control the first generation, and summer corn is used to control the second generation. The pesticide should be applied during the peak period of egg hatching and the end of the heart leaf stage of corn. Further tests can be carried out according to the requirements of the agreement. Record the quantity and date of each application. 3.3.4 Use of dosage and capacity
Apply the pesticide according to the requirements of the agreement and the dosage indicated on the label. The content of active ingredients in the pesticide is usually expressed as g/hm (g/hectare). When used for spraying, the multiple of application and the amount of liquid per hectare (L/hm2) should be recorded at the same time. 3.3.5 Requirements for information on drugs used to control other pests and diseases If other drugs are used, drugs that have no effect on the test drugs and test objects should be selected, and all plots should be treated uniformly. They should be used separately from the test drugs and control drugs to minimize the interference of these drugs. Record accurate data on the application of such drugs.
4 Investigation, recording and measurement methods
4.1 Meteorological and soil data
4.1.1 Meteorological data
During the test, data on rainfall (precipitation type, daily rainfall in mm) and temperature (daily average temperature, maximum and minimum temperature in °C) should be obtained from the test site or the nearest meteorological station. Adverse climatic factors that affect the test results during the entire test period, such as severe or long-term dry spells, violent droughts, and ice poverty, must be recorded. 4.1.2 Soil data
Record data such as soil type, soil fertility, crop yield level, weed coverage, etc. 4.2 Survey method, time and frequency
4.2.1 Survey method
Survey 50 corn plants in each plot, count the rate of damaged plants, check the number of live insects on the damaged plants, and calculate the relative control effect by comparing with the control area. 4.2.2 Survey time and frequency
Survey at the end of the pest generation, that is, 10 to 15 days after the application of the pesticide. 4.2.3 Calculation method of drug efficacyWww.bzxZ.net
The drug efficacy is calculated according to formula (1), formula (2), formula (3), and formula (4): Damaged plant rate (%) ==
Control effect (%) =
Where: CK blank control area damaged plant rate; PT—-damaged plant rate in the pesticide treatment area.
Number of affected plants
Total number of plants investigated
CK- PT
（2）
GB/T 17980. 6--2000
Number of live larvae
Live insect rate (%) = Total number of affected plants investigated
Larvae control efficiency (%) =
Where: CK,—Live insect rate in blank control area; PT---
—Live insect rate in pesticide treatment area.
4.3 Direct impact on crops
CK, PT
× 100
It is necessary to check whether the pesticide has any damage to the crops, and record the type and degree of damage. In addition, the beneficial effects on the crops (such as accelerated maturity, increased vitality, etc.) should also be recorded.
Record pesticide damage in the following ways:
a) If the pesticide damage can be counted or measured, it should be expressed in absolute values, such as plant height. b) In other cases, the extent and frequency of pesticide damage can be estimated by the following two methods: 1) Record the pesticide damage situation in each plot according to the pesticide damage classification method, expressed as one, ten, ten ten, ten ten ten, ten ten ten ten. Pesticide damage classification method:
·No pesticide damage,
Ten: Mild pesticide damage, does not affect the normal growth of crops; Ten ten: Moderate pesticide damage, can be restored, will not cause crop alkali production: Ten ten ten: Severe pesticide damage, affects the normal growth of crops, causes a certain degree of loss in crop yield and quality; Ten ten ten ten: Severe pesticide damage, crop growth is hindered, and crop yield and quality loss are serious. 2) Compare the pesticide treatment area with the blank control area to evaluate the percentage of pesticide damage. At the same time, the pesticide damage symptoms of crops (dwarfing, chlorosis, deformity, etc.) should be accurately described. 4.4 Impacts on other organisms
4.4.1 Impacts on other pests and diseases
Any impacts on other pests and diseases should be recorded, including beneficial and unbeneficial impacts. 4.4.2 Impacts on other non-target organisms
Record any impacts of the pesticide on wildlife and beneficial insects. 4.5 Product quality and yield
If measurement is required, the yield of each plot is calculated as the weight of 50 fruit ears. Plants that have fallen due to pests should be recorded separately from healthy plants. 5 Results
The test data were analyzed using the Duncan's new multiple range (DMRT) method, and corresponding biostatistical methods were used in special cases. A formal test report was written, and the test results were analyzed and evaluated. The test report should list the original data. 3368
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