This part specifies the methods and basic requirements for field efficacy plot tests of insecticides for controlling apple aphids. This part applies to field efficacy plot tests and efficacy evaluation of insecticides for controlling apple aphids for registration purposes. NY/T 1464.5-2007 Guidelines for Field Efficacy Tests of Pesticides Part 5: Insecticides for Controlling Apple Aphids NY/T1464.5-2007 Standard download decompression password: www.bzxz.net
This part specifies the methods and basic requirements for field efficacy plot tests of insecticides for controlling apple aphids. This part applies to field efficacy plot tests and efficacy evaluation of insecticides for controlling apple aphids for registration purposes.

TCS65.100
Agricultural Industry Standard of the People's Republic of China
NY/T 1464.5—2007wwW.bzxz.Net
Guidelines on efficacy evaluation of pesticides Part 5: Insectides against apple woolly aphid2007-12-18 issued
2008-03-01 implementation
The Ministry of Agriculture of the People's Republic of China issued
NY/T1464 "Guidelines for Field Efficacy Tests of Pesticides" is a series of standards, totaling 26 parts: Part 1: Insecticides for controlling locusts;
Part 2; Insecticides for controlling rice water weevils-Part 3; Insecticides for controlling cotton stink bugs; Part 4: Insecticides for controlling pear yellow aphids
Part 5: Insecticides for controlling apple earthworms: Part 6: Insecticides for controlling vegetable thrips; Part 7: Fungicides for controlling tobacco anthracnose: Part 8: Fungicides for controlling tomato virus diseases; Part 9: Fungicides for controlling pepper virus diseases; Part 10: Fungicides for controlling mushroom wet bubble disease: Part 11: Fungicides for controlling banana black spot; Part 12: Fungicides for controlling grape powdery mildew; Part 13 Part: Fungicides for the control of grape anthracnose; Part 14: Fungicides for the control of rice damping-off; Part 15: Fungicides for the control of wheat scab; Part 16: Fungicides for the control of wheat root rot; Part 17: Herbicides for the control of green sub-weeds; Part 18: Herbicides for the control of sesame weeds; Part 19: Herbicides for the control of weeds in wolfberry fields; Part 20: Herbicides for the control of weeds in tomato fields; Part 21: Herbicides for the control of weeds in cucumber fields; Part 22: Herbicides for the control of weeds in dachu fields; Part 23: Herbicides for the control of weeds in first hunting fields; Part 24: Herbicides for the control of weeds in red beans; Part 25: Herbicides for the control of weeds in tobacco seedbeds, Part 26: Test of cotton wilt accelerators.
This part is Part 5 of the "Field Efficacy Test of Pesticides". This part was proposed by the Crop Management Department of the Ministry of Agriculture of the People's Republic of China and is under the jurisdiction of the Pesticide Testing Institute of the Ministry of Agriculture. The main drafters of this part are Tao Lingmei, Chen Hanjie, Qiu Guisheng, Li Xiaojun, Ji Lili, Zhang Nan, Wang Xiaojun, NY/T1464.5-2007
1 Scope
Guidelines for field efficacy tests of pesticides
Part 5: Insecticide control of woolly apple aphids
NY/T 1464.5—2007
This part specifies the methods and basic requirements for field efficacy plot tests of insecticides for control of woolly apple aphids (Eriusomu lanigerum). It does not apply to the registration of insecticides for control of woolly apple aphids. Field efficacy plot tests and efficacy evaluation 2 Test conditions
2.1 Test objects and crops
The test object is woolly apple aphid. The main developmental stages of woolly apple aphids in the test plots shall be recorded. The test crop is apple tree, and it is advisable to select the local main crop variety, and record the variety name, growth period, and planting density. 2.2 Test site
The test should select plots with moderate or severe damage to apple aphids. The cultivation conditions of the test site (such as soil type, fertilizer cultivation, etc.) should be consistent and comply with local good agricultural practices. 3 Test design and arrangement
3.1 Test treatment
3. 1. 1 Agent
3.1.1.1 Test agent
The test agent should be set with 3 or more treatment doses, or set according to the requirements of the agreement. Record the generic name (in Chinese or English) or code of the drug, dosage form, content, manufacturer and treatment dosage (expressed in effective concentration mg/kg or mg/L) 3.1.1.2 Control drug
The control drug must be a registered drug that has been proven to be effective in practice. Its type and mode of action are similar to those of the test drug. The control drug is used according to the local conventional application amount
The test drug is a single agent, and at least another local commonly used single agent is set as the control drug; when the test drug is a mixture, each single agent of the mixture is used as the control drug. It must also contain a local commonly used drug as the control drug. Record the generic name, dosage form, content, manufacturer, and application amount of the control drug. 3.1.2 Blank control
Set the treatment without drug as the blank control.
3.2 Number of repetitions
Each treatment is repeated at least 4 times.
3.3 Plot arrangement
3.3. 1 Plot area
There shall be no less than 10 trees in each plot.
A protective row or isolation belt shall be set between plots, and 1/2 of the area of ​​the protective row or isolation belt shall be treated in the same way as the adjacent plots. Record the plot area and the width of the row or protection belt between plots. 3.3.2 Plot arrangement
Each plot shall be arranged in random blocks, and the plot arrangement diagram shall be recorded. NY/T 1464.5 2007
3.4 ​​Requirements for agents used to control pests other than the test object If other agents are needed to control diseases, weeds and weeds other than the test object during the test, other test agents and agents that have no effect on the test object shall be selected, and they shall be used separately from the test agents and control agents, and all test plots shall be treated equally. Record the exact data of the application of such agents (name of agent, content, dosage form, manufacturer, application dosage, application method, and application time). 4 Application of pesticides
4.1 Application method
Perform according to the agreement. Application should comply with local good industry practices.4.2 Application equipment
Use the equipment that is usually used in the orchard, or select the equipment according to the agreement. Record the type and operating conditions of the equipment used.4.3 Application time
Application is usually carried out at the early stage of apple aphid infestation (the beginning and peak period of migration of 1st instar nymphs), or according to the agreement. Record the application time (date). 4.4 Application times
Perform according to the agreement and record.
4.5 Application amount
Determine the application amount according to the application method, crop type and growth period, and plot area, or according to the requirements of the agreement: record the application amount, expressed as 1.lirn, when spraying with ding. At the same time, record the multiple of the application and the average application amount per plant. 5 Investigation
5.1 Effect investigation
5.1.1 Investigation method
Investigate 2→3 plants in each plot, and mark a branch in each of the five directions of east, west, south, north, and center. The marked branches are small and the number of all live apple aphids on them is investigated and recorded. When investigating the base number, the number of insects in each plot is not less than 100. When the tree is small, the number of insects on the whole tree can be investigated and recorded. During the investigation, the darkening of the color of the apple aphid is considered dead, and the white and fresh ones are considered alive.
2 Survey time and frequency
Survey the base number before application (number of live insects before application), and investigate the number of surviving insects on the 7th, 14th and 21st day after application (number of live insects after application). According to the test requirements and the dosage of the pesticide, increase the number of surveys or extend the survey time. 5.2 Direct effect on crops
Observe whether the pesticide has any damage to the crops. If there is any damage, record the symptoms, type and degree of damage: In addition, record the beneficial effects on the crops (such as accelerated maturity, increased yield, etc.). Record the damage in the following way:
If the damage can be counted or measured, it should be expressed as an absolute value. In other cases, the degree and percentage of damage can be estimated by the following two methods: 1) According to the damage classification method, record the damage situation in each plot. The damage classification method is expressed as 1, 2, 3, 4, and 5:
-: No damage;
- Mild damage, visible damage, no impact on normal growth of crops 1: Obvious damage, can be recovered, will not cause crop yield reduction; 110: Severe damage, affecting normal growth of crops, causing a certain degree of loss to crop yield and quality, generally requiring compensation for part of the economic loss;
-: Severe damage, crop growth is hindered. Crop yield and quality loss are serious, and economic losses must be compensated. 2
NY/T 1464.5 2007
2) Compare the treated area with the blank control area to evaluate the damage percentage. When consulting, the crop damage symptoms (dwarfing, chlorosis, deformity, etc.) should be accurately described, and photos and videos of the actual objects should be provided. 5.3 Impact on other organisms
5. 3. 1 Impact on other pests and diseases
Any impact on other pests and diseases should be recorded, including beneficial and unbeneficial impacts. 5.3.2 Impact on other non-target organisms
Record the impact of the pesticide on endogenous organisms and beneficial insects in the test area. 5.4 Other information
5.4.1 Meteorological data
Adjust and record the meteorological data of the test site during the entire test period, including rainfall (rainfall type and oral rainfall, expressed in mm) and temperature (daily average humidity, maximum and minimum temperature, expressed in mm), etc. 5.4.2 Soil culture data
Investigate and record soil type, soil fertility, crop yield level, and crop or weed coverage. 6 Calculation formula and data analysis
Calculate the control effect according to the following formula, and use the biostatistical method (I) MR method) for statistical analysis. r =
Wherein:
PT——control effect, unit is white score (%); CK.X PT.1
CK,X FT,!
PT——number of live insects before application in the treatment area, unit is individual; PT1——number of live insects after application in the treatment area, unit is individual: CKa
number of live insects before application in the empty control area, unit is individual; CKi
number of live insects after application in the solid white control area, unit is individual. 7 Evaluation of the agent and report writing
Analyze and evaluate the agent in reverse order according to the results, write a formal test report, and list the original data. 3
NYI T1464.5-2007
People's Republic of China
Agricultural Industry Standard
Guidelines for Field Efficacy Tests of Pesticides
Part 5: Insecticides for Control of Woolly Apple Aphids NY/T 1464.5—2007
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