NY/T 603-2002 Technical regulations for breeding of double-low conventional rapeseed seeds of Brassica juncea and Brassica juncea
Some standard content:
1S E5.020-20
Agricultural Industry Standard of the People's Republic of China
NY/T6032002
Technical procedure for mulliplication of double low rapeseed of Brassica juncea2002-11- 05Promulgated
Implementation on 2002-12-20
Ministry of Agriculture of the People's Republic of China
This standard was proposed by the Market and Whole Information Department of the Ministry of Agriculture, NY/T 603-2002
This standard was drafted by the Quality Supervision and Inspection Center of Crop Seeds of the Ministry of Agriculture [Wuhan], and finalized by the Seed Management Station of Hebei Province. This standard mainly refers to the following persons: Wu Qingfeng, Liu Hanju, Ting Lianbing, Fang Shangying 1 Standard Use
Technical Regulations for Breeding of Cabbage-type and Mustard-type Double Low Conventional Rapeseed Seeds
The standard specifies the relevant technical methods and procedures for breeding cabbage-type and double low conventional rapeseed seeds. NY/T 603—2002
The standard is applicable to the breeding and production of H-type and H-type conventional rapeseed seeds, Ding type and Dashan type seeds, and is also applicable to the breeding and production of non-effective and non-standard oilseed varieties. 2 Normative Reference Documents
The provisions in the following documents become the provisions of this standard through reference in this standard. From the date of the reference document, all subsequent changes (excluding those included in the package or revision) shall apply to this standard. However, all parties to the agreement on the standard may use the latest version of these documents. For all reference documents not dated, the latest version shall apply to this standard. GB/3343 [All Identification] Inspection Procedure for Seeds of Agricultural Crops G4417.2 Oil Seeds of Oil Crops NYT91 Determination of Glucose Content in Rapeseed by Gas Chromatography (formerly GBU211988) NY414 ISO5161 Determination of Glucose Content in Rapeseed by High Performance Liquid Chromatography 3 Terms and Definitions The following terms and definitions apply to this standard. 3.1 Double-low rapeseed double luw 3. 2
Conventional rapeseed: seed of rapeseed is produced by release rather than hybridization, and the offspring can maintain the natural characteristics of the variety. Seeds for breeding or rejuvenation are typical of the species: waiting for birth, this is the first batch of family seeds with stable genetic traits or parent varieties. Used for natural seed and seed: original seed lansie seexl
The first generation of effective seed producers have been planted, and the original seed production technology has been well produced according to the original seed quality standards, and the seeds are widely used in the field. Seed
The strategy of using original seed to breed the first generation of seeds, using Ding Da for production, NF/T 603-2D02
4 Commercial method
Use the two-centimeter system <lineage, original seed solid> lineage comparison method to breed breeder seeds and original seeds. That is, use the single plant selection and lineage comparison method (lineage comparison method) to breed the original seeds. Small original seeds are used to breed large four-state varieties. 5 Commercial seed breeding plan
5.1 Single sample selection
5.1.1 Seed field for single plant selection
Single plant selection is carried out in the seed breeding or in the original seed market with strict isolation (such as isolation room) 5. 1.2 Selection period and quantity
The selection of individual plants is carried out in the seedling stage, the inflorescence stage and the maturity stage according to the typical characteristics and appearance. More plants should be selected in the early stage, and the selected plants should be marked for further selection in the inflorescence and maturity stages. During the early stage, 15-200 plants are sent, and 30-100 plants selected in the seedling stage are selected for flower phase and bagged; during the inflorescence stage, 70 plants selected in the flowering stage are selected, and the selected plants are selected for bagging. Quality analysis is conducted. Based on the economic traits and quality analysis results, the single plants with good quality and excellent economic traits are selected. 5-1.3 Selection criteria
5.1.3.1 Seedling stage
Select according to the typical characteristics of the source variety, leaf shape, leaf color, wax powder thickness, heart leaf color, whether the prickles are notched, stem color, seedling growth habit, growth potential, appearance and early return, etc., and mark the selected items. 5-1.32 Flowering period
Based on the original variety and plastic characteristics, select the selected individuals in the seedling stage for traits such as plant quotient, color, number of branches, flower shape and color, and consistency of tree flowering period and final flowering period, mark and bag the plants with typical characteristics of the original variety, and remove the marks on the individuals that are not selected in the seedling stage but flowered
5.1.3.3 Maturity period
Based on the original variety and typical characteristics, select the individual plants in the seedling stage for traits such as plant height, thickness, branch length, branch position, main inflorescence length, fruiting rate, silique shape and length, number of individual fruits, consistency, and maturity period, and select the individuals with good yield, disease resistance and consistency for marking, and remove the marks in advance on the individuals that are not selected in the flowering period but flowered in the maturity period. 5.1.3-4 Seed testing and quality inspection
After harvest, the varieties in the mature stage should be placed in a well-ventilated room for 10 days before going out for testing. The quality inspection includes dry grain weight, single head yield, color, etc. The seeds with high glycerol content and monohydrate content are selected according to the method of Y1919167-1. 5.2 Planting
5.2.1 Seeds from the original
The selected plants
5.2.2 Planting
The planting of the plants should be carried out in a grid isolation system, using barrier isolation (mountain, small field, lake island or human isolation room or distance (more than 100m), small area planting should be carried out in isolation: metal frame, plastic and glass cover (aperture less than 0.38m).
5.2.3 Planting
Each plant selected in the previous season should be planted in a core area (sample) in the sample system, each area, 10 rows to 10 rows, if the original variety has been planted, it will be planted as a control. The planting method is to use transplanting or direct planting. The active strains are used. The soil is carefully prepared and the base is applied, but the soil is not allowed to be made of farmyard manure made from oilseed stems and fruit shells. The typical single plant is selected, and the plant spacing is several cm × W cm. The transplanting office should strengthen management to prevent pests and diseases: 5.2.4 Selection period
NY/T603—2002
According to the characteristics of the original variety, selection is carried out at the seedling stage, warning stage, germination stage and maturity stage. The typical fast-growing system, disease signs, inferior system,
5.2.5 Selection criteria
5-2.5.1 Seedling stage
Observe the leaf shape, leaf color, wax powder, thorn flying, petiole length, young stem color, paleobiosis, growth potential, winter sensitivity, etc.
5. 2. 5.2 General period
Observe the shape of petiole, leaf color, number of leaf pieces, color selection, growth characteristics, growth potential, cold tolerance, etc. 5.2.5.3 Flowering period
Observe the uniformity of flowering, flowering period and growth period, check the samples with early maturity or poor uniformity, and also observe the size and color of early flowering, overlapping conditions, and carefully sort according to habits, etc. For each sample selected during the flowering period: separate them after weeding and removing inferior ones. 5-2.5-4 Maturity period
The selected strains are determined by the morphology of siliques, the skin of the main inflorescence, the density of fruits, lodging resistance, disease resistance and other characteristics during the heat period, and the number of schizophyllum diseases and the number of schizophyllum diseases.
5. 7. 6 Harvest,The selected lines in the final selection and purchase period are harvested according to length, tested, threshed and analyzed for quality. The test and quality analysis items include: height, branch position, number of effective branches per plant, length and quality of inflorescence, number of effective siliques in the main inflorescence, number of siliques in the whole plant, number of fruits per fruit, whole weight, yield, seed color, oil content, fat content, erucic acid and linalool content, etc. NYTS1 and 1SO9167-1 are used for tocopherol and phytochemical testing. Then the test and quality analysis results are compared with the original breeding standards, and the lines with traits that meet the original variety standards and double low indicators are determined as the final selection lines. Finally, the seeds of the final selection lines are mixed to form the seedlings. Some varieties are planted and stored in a dryer after being fully dried for ten years, so that they can be used year by year. 6. 1 Seeds from the source of breeding. 6.2 Sun-isolated natural barrier, small basins or tidal islands, or the distance from the isolation zone (more than 1 heart) for two consecutive years, the oilseed plots are used as purple fungi. Other cruciferous crops such as oilseed, mustard, etc. are not allowed to be planted in the isolation zone. 6.3 Before planting, the land should be carefully prepared, and sufficient base should be applied. Organic fertilizers should be used as the main fertilizer, and organic fertilizers, companion fertilizers, and fertilizers should be applied in combination. Sow in time and cultivate strong. The planting method is to adopt direct seeding by transplanting. When the belt transplanting method is used, the seed donation amount of the seedbed is 4/m~0.5/m, and the ratio of the seedbed to the day is (1:5)~(1:15). The planting density is 12-15 cubic meters per hectare. When the direct splicing method is used, the seed donation amount is 6kg/lum*~7.5kg/hm, and the planting density is 15-18 cubic meters per hectare. After the strong pruning, the area of the area of the dam was slightly affected. Follow the treatment of pests and diseasesbZxz.net
6.4 Weeding and removing inferior plants
Weeding is carried out four times during the whole growth period, at the reed stage, the vine stage, the flowering stage and the vine stage: the seedling stage and the vine stage. The focus of the work is to remove the rapeseed, the self-growing rapeseed and other 10 flowering crops in the area; during the flowering and ripening stages, weeds, variants and foreign varieties in the natural area should be removed; from the ripening stage to the harvest, inferior plants and diseased plants should be removed, and the weed rate after weeding should not exceed 2%. 6.5 Harvest After harvesting or ripening, the seeds are strictly removed and the qualified blocks are mixed, threshed, filtered, dried, and selected for inspection. The seeds that meet the seed quality and double low index are the fan seeds, which are packed into bags and stored in a dry and windy place. 6.6 Seed inspection and quality testing After the seeds are selected: Sampling and inspection are carried out in accordance with GB/T3543 (the stored samples are sent for sampling and inspection in accordance with NY/97 and IS0157-1NY/T 6D3—2002
Carry out acid and sulfur test. The original seed should meet the requirements of GB07.2, and the sulfur should be reduced to the requirements of Y414 before it can be used as a commodity. The quality and efficiency of the seed should be tested by a qualified testing agency.
7 Seed cultivation for field use
7.1 Seed source
Original seed.
1.2 Two-time interval
7.3 Planting
Same as 6.3.
7.4 Removal of relatives and inferior
Same as 6.4-
. 5 Harvest and storage
To 6.5.
t-6 Seed inspection and quality inspection
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