This standard specifies the test method for Salmonella in food. This standard is applicable to the test for Salmonella in various types of food and food poisoning samples. GB/T 4789.4-2003 Food Hygiene Microbiological Test Salmonella Test GB/T4789.4-2003 Standard Download Decompression Password: www.bzxz.net

ICS07.100.30
National Standard of the People's Republic of China
GB/T4789.4—2003
Replaces GB/T4789.4—1994
Microbiological examination of food hygiene
Examination of Salmonella
Promulgated on August 11, 2003
Ministry of Health of the People's Republic of China
Standardization Administration of the People's Republic of China
Implementation on January 1, 2004
GB/T4789.4—2003
This standard revise GB/T4789.4—1994 "Microbiological examination of food hygiene--Examination of Salmonella".
Compared with GB/T4789.4-1994, this standard has the following major revisions: The format and text of the standard text are revised in accordance with GB/T1.1-2000. - Modify and standardize the "equipment and materials" in the original standard. This standard is implemented from the date of implementation, and GB/T4789.4-1994 is abolished at the same time. This standard is proposed and managed by the Ministry of Health of the People's Republic of China. The drafting units of this standard are: Jiangxi Provincial Health and Epidemic Prevention Station, and the Institute of Nutrition and Food Safety of the Chinese Center for Disease Control and Prevention. The main drafters of this standard are: He Xiaoqing, Ran Lu, Fu Ping, Yang Baolan, and Yao Jinghui. This standard was first issued in 1984, revised for the first time in 1994, and this is the second revision. 20
1 Scope
Food Hygiene Microbiological Examination
Salmonella Examination
This standard specifies the test methods for Salmonella in food. This standard is applicable to the test of Salmonella in various types of food and food poisoning samples. 2 Normative references
GB/T4789.4—2003
The clauses in the following documents become the clauses of this standard through reference in this standard. For all dated references, all subsequent amendments (excluding errata) or revisions are not applicable to this standard. However, parties to an agreement based on this standard are encouraged to study whether the latest versions of these documents can be used. For all undated references, the latest versions are applicable to this standard. GB/T4789.28--2003 Food hygiene microbiology inspection staining method, culture medium and reagents 3 Equipment and materials
3.1 Refrigerator: 0℃~4℃.
3.2 Constant temperature incubator: 36℃±1℃, 42℃. 3.3 Microscope: 10×~100X×.
3.4 ​​Homogenizer or sterile mortar.
3.5 Shelf drug volume: 0g~500g, accurate to 0.5g. 3.6 Sterile wide-mouth bottle: 500mL.
3.7 Sterile conical flask: 500mL, 250mL. 3.8 Sterile pipette: 10mL (with 0.1mL scale). 3.9 Sterile culture dish. 90mm.
3.10 Sterile small test tube: 3mm×50mm.
3.11 Sterile capillary tube.
4 Culture medium and reagents
Buffer protein water (BP): in accordance with 4.12 of GB/T4789.28-2003. 4.1
4.2 Magnesium chloride malachite green (MM) enrichment solution: in accordance with 4.13 of GB/T4789.28--2003. 4.3 Sodium tetrasulfate brilliant green (TTB) enrichment solution: in accordance with 4.14 and 4.15 of GB/T4789.28-2003. Selenite cystine (SC) enrichment solution: in accordance with 4.16 of GB/T4789.28-2003. 4.4
Bismuth sulfite agar (BS): in accordance with 4.19 of GB/T4789.28-2003. 4.5
DHL agar: in accordance with 4, 20 of GB/T4789.28-2003. 4.6
HE agar: in accordance with 4.21 of GB/T4789.28-2003. 4.7
WS agar: in accordance with 4.23 of GB/T4789.28-2003. 4.8
4.9SS agar: in accordance with 4.22 of GB/T4789.28-2003. Tri-sugar iron agar: in accordance with 4.26 and 4.27 of GB/T4789.28-2003. 4.10
Protein stale water, indigo matrix reagent: in accordance with 3.13 of GB/T4789.28-2003. Urea agar (pH7.2): in accordance with 3.15 of GB/T4789.28-2003. Potassium cyanide (KCN) culture medium: in accordance with 3.16 of GB/T4789.28-2003. Amino acid decarboxylase test culture medium: in accordance with 3.12 of GB/T4789.28-2003. 21
GB/T4789.4—2003
Sugar fermentation tube: in accordance with 3.2 of GB/T4789.28—2003. 4.15
ONPG medium: in accordance with 3.3 of GB/T4789.28—2003. 4.16
Semisolid agar: in accordance with 4.30 of GB/T4789.28-2003. Sodium malonate medium: in accordance with 3.7 of GB/T4789.28-—2003. 4.18
Salmonella factor serum: 26 types for preliminary typing; 57 types for further typing; 163 types for detailed typing. 5 Inspection procedures
See Figure 1 for the inspection procedure for Salmonella.
Pre-enrichment method
Frozen meat, egg crystals, dairy products
and other processed foods
25g+BP225mL
36℃±1Generally 4h, dry strong products 18h~24h
10mL+MM
(or TTB)100mL
10mL+SC100mL
18h~24h36℃±1
H,S+indigo matrix
urea-KCN-
Lysine+
Salmonella chisel
Serological test
18h～24h
40h~48h
Pick suspicious colonies
Direct bacterial enrichment method
Fresh meat, fresh eggs, fresh milk or other unprocessed foods 25+sterile physiological
saline 25mL, make sample homogenization
sample hook solution 25mL+
MM (or TTB) 100mL|| tt||18h~24h
Test sample solution 25mL+
SC100mL
DHL (or HE, WS, SS)
36℃±18
TSI (slant, bottom layer, gas production, H, S), protein water (Xin matrix), urea (pH7.2), KCN, lysine
H, S+knock matrix+
urea-KCN-
carboxylic acid+
glycanol, sorbitol
Salmonella
Serological test
H,S-indigo matrix-
Urea-KCN-
Lysine+/-
Non-Salmonella
18hm24h
18h~24h
Not as described on the left
Various reaction results
Non-Salmonella
6 Operation steps
6.1 Pre-enrichment and enrichment
GB/T4789.4—2003
Frozen meat, eggs, dairy products and other processed foods should all be pre-enriched. Take 25g (mL) aseptically and add it to a 500mL wide-mouth bottle filled with 225mL buffered protein water. Solid food can be crushed with a homogenizer at 8000r/min~10000r/min for 1min, or ground with sterilized sand in a mortar. Powdered food can be ground with a sterilized spoon or glass rod to emulsify, incubate at 36℃±1℃ for 4h (dry eggs for 18h~24h), transfer 10mL, transfer to 100mL magnesium chloride malachite green enrichment solution or sodium tetrasulfate brilliant green enrichment solution, and incubate at 42℃ for 18h~24h. At the same time, take another 10mL and transfer to 100mL selenite cystine enrichment solution, and incubate at 36℃±1℃ for 18h~24h.
Fresh meat, fresh eggs, fresh milk or other unprocessed foods do not need to be pre-enriched. Take 25g (25mL) of each and add 25mL of sterile physiological saline to make a sample homogenate according to the previous method; take 25mL and inoculate it into 100mL of magnesium chloride malachite green enrichment solution or sodium tetrasulfonate brilliant green enrichment solution, and culture it at 42℃ for 24h; take another 25mL and inoculate it into 100mL of selenite cystine enrichment solution, and culture it at 36℃±1℃ for 18h~24h. 6.2 Separation
Take 1 loop of enrichment solution and streak it on a bismuth sulfite agar plate and a DHL agar plate (or HE agar plate, WS or SS agar plate). The two enrichment solutions can be streaked on the same plate at the same time. The plates were cultured at 36°C ± 1°C for 18 h 24 h (DHL, HE, WS.SS) or 40 h ~ 48 h (BS), and the colonies grown on each plate were observed. The colony characteristics of Salmonella I, IIII, IV, V, VI and Salmonella III on each plate are shown in Table 1. Table 1 Colony characteristics of various groups of Salmonella on various selective agar plates Selective agar plates
Bismuth sulfite agar
DHL agar
HE agar
WS agar
SS agar
6.3 Biochemical tests
Salmonella I, I, N, V, I
Salmonella III (i.e. Arizona strain)
The colonies of hydrogen sulfide-producing strains are black with metallic luster, brown or black with metallic luster and gray, and the culture medium around the colonies may be black or brown; some strains do not produce hydrogen sulfide and form gray-green colonies, and the surrounding culture medium remains unchanged
Colorless and translucent, the center of the colonies producing hydrogen sulfide is black or almost completely black
Blue-green or blue, most strains produce hydrogen sulfide, and the colonies are Black or almost completely black in the center
Colorless and translucent, some colonies of hydrogen sulfide-producing strains have black centers, but not as obvious as the above culture media
Lactose-retardant positive or negative strains are the same as Salmonella 1Ⅱ, IV, V, V: lactose-positive strains are pink with black centers
Lactose-positive strains are yellow with black or almost completely black centers, lactose-retardant positive or negative strains are blue-green or blue with black or almost completely black centers. Lactose-retardant positive or negative strains are the same as Salmonella I, I, IV, V, V, and lactose-positive strains are pink with black centers, but when there is no black formation in the center, it cannot be distinguished from Escherichia coli
6.3.1 Pick several suspicious colonies directly from the selective agar plate and inoculate them into triple sugar iron agar respectively. The reaction results of common genera and species of Enterobacteriaceae in triple sugar iron agar are shown in Table 2.
Table 2 Reaction results of each genera of Enterobacteriaceae in triple sugar iron agar Slant
Hydrogen sulfide
Possible genera and species
Salmonella, Citrobacter freundii, Proteus, Edwardsiella lentus
Salmonella III, Citrobacter freundii, Proteus vulgaris Salmonella, Escherichia coli, Hafnia alveolar, Morganella, Providencia
GB/T4789.4—2003
Note, ten positive; one negative; ten/one mostly positive, a few negative. Table 2 (continued)
Hydrogen sulfide
Possible bacterial genera and species
Salmonella enterica, Salmonella gallinarum, Shigella, Escherichia coli, Hafnia alveolar, Kugenella, Providencia, Escherichia coli, Enterobacter, Klebsiella, Serratia, Citrobacter freundii
Table 2 shows the strains that only produce acid on the slant and are negative for hydrogen sulfide (H, S) in triple sugar iron agar It can be ruled out. Other reaction results may be Salmonella, but they may not be Salmonella. 6.3.2 While inoculating the three-sugar iron agar, inoculate one tube each of protein aged water (for indigo test), urea agar (pH7.2), potassium cyanide (KCN) culture medium, lysine decarboxylase test culture medium and control culture medium. Incubate at 36℃±1℃ for 18h~24h, which can be extended to 48h if necessary. Determine the results according to Table 3. According to the reaction sequence classification, the results of Salmonella should belong to A1, A2 and B1. The other five reaction results can be ruled out.
Table 3 Preliminary identification of various genera of Enterobacteriaceae by biochemical reactionReaction number
Hydrogen sulfide
Indigo matrix
pH7.2Urea
Potassium cyanide
Lysine decarboxylase
Note 1: The bottom layer of triple sugar iron agar all produces acid; those that do not produce acid can be excluded; the production of acid and gas on the inclined surface is not limited. Note 2: One of KCN and lysine can be selected, but if the result cannot be determined, the other one still needs to be supplemented. Note 3: Ten means positive: Ten means negative Ten/One means majority positive and minority negative, determine the bacterial genus
Salmonella
Salmonella (rare) Slow
Edelweiss
Citrobacter freundii,
Proteus mirabilis
Proteus vulgaris
Salmonella, Escherichia coli
Salmonella paratyphi A||t t||Bacteria, Escherichia coli, Shigella
Escherichia coli, Escherichia coli, Shigella
Klebsiella
Enterobacter cloacae, Citrobacter freundii
Morganella morganii, Prophy
Densella
6.3.2.1 Reaction No. A1: Typical reaction is determined as Salmonella. If one of the three items of urea, potassium cyanide and lysine is abnormal, it can be determined as Salmonella according to Table 4. If two items are abnormal, it can be determined as Citrobacter freundii according to A3. Table 4
PH7.2 Urea
Potassium cyanide (KCN)
Note: Ten means positive, one means negative. 24.
Lysine
Judgment result
Salmonella paratyphi A (serological identification results are required)Salmonella IV or V (must meet the biochemical characteristics of this group)Salmonella individual variants (serological identification results are required)6.3.2.2Reaction No. A2: Perform mannitol and sorbitol tests again, and judge the results according to Table 5. Table 5
Mannitol
Sorbitol
Note, ten means positive, one means negative. Judgment result
GB/T4789.4—2003
Salmonella indigo matrix positive variant (serological identification results are required)Edwardella lentus
6.3.2.3Reaction No. B1: Perform ONPG again. ONPG ten is Escherichia coli, ONPG one is Salmonella. At the same time, Salmonella should be lysine ten, but Salmonella paratyphi A should be lysine one. 6.3.2.4 If necessary, identify the biochemical group of Salmonella according to Table 6. Table 6 Identification of each biochemical group of Salmonella
Dulcitol
Sorbitol
Salmon
Malonate
Potassium lysate
Note, ten means positive, one means negative. 6.4 Serological typing identification
6.4.1 Preparation of antigen
Generally, 1.5% agar slant culture is used as the antigen for slide agglutination test. When V
O serum does not agglutinate, inoculate the strain on a medium with a higher agar content (such as 2.5% to 3%) and then check again: If the presence of Vi antigen prevents the O agglutination reaction, pick up the bacterial moss and make a concentrated bacterial solution in 1mL of physiological saline, boil it on the flame of an alcohol lamp and then check again. When the H antigen is underdeveloped, inoculate the strain in the center of a 0.7% to 0.8% semi-solid agar plate, and when the colony spreads and grows, take bacteria from its edge for inspection; or pass the strain through a small glass tube filled with 0.3% to 0.4% semi-solid agar once or twice, take bacteria from the far end for culture and then check again.
6.4.Identification of 20 antigens
Use A~F polyvalent O serum for slide agglutination test, and use physiological saline as control. Those that self-agglutinate in physiological saline are rough strains and cannot be typed.
Those agglutinated by A~F polyvalent 0 serum are subjected to agglutination test with 04;03, 10;07;0809;02 and 011 factor sera in sequence. According to the test results, the 0 group is determined. For strains agglutinated by 03 and 10 sera, agglutination test is performed with 010, 015, 034.019 single factor sera, and the 0 group is determined according to the test results. For strains agglutinated by O3 and 10 sera, agglutination test is performed with 010015, 034, 019 single factor sera to determine the E1, E2, E3, and E4 subgroups. The final determination of each O antigen component should be based on the test results of O single factor serum. If there is no 0 single factor serum, two 0 composite factor sera should be used for verification. For those who are not agglutinated by A to F multivalent 0 sera, first use 9 multivalent 0 sera among the 57 or 163 Salmonella factor sera for examination. If one of the sera agglutinates, use the O group sera included in this serum to examine one by one to determine the ○ group. The ○ factors included in each multivalent ○ serum are as follows:
O multivalent 1A, B, C, D, E, F group (including 6, 14 groups)0 multivalent 213, 16, 17, 18, 21 groups
0 multivalent 328, 30, 35, 3839 groups
0 multivalent 440, 41, 42, 43 groups
GB/T4789.4—2003
0 multivalent 544, 45, 47, 48 groups
0 multivalent 650, 51, 52, 5 Group 3
0 Multivalent 755.56.5758 Group
0 Multivalent 859, 60, 61, 62 Group
0 Multivalent 96365, 66, 67 Group
6.4.3 Identification of H antigen
For uncommon bacterial types, first use 8 polyvalent H sera among the 163 Salmonella factor sera for inspection. If one or two of the sera agglutinate, use the various H factor sera included in this one or two sera to check one by one to determine the H antigens of the first and second phases. The H factors included in the 8 polyvalent H sera are as follows: H polyvalent 1a, b, c, d, i
H polyvalent 2eh, enxenzisfg.gmsgpu.gp.gq, mt.gzaH polyvalent 3k, r.yz, zo, lv.lw, lz, lz2, lzaoH polyvalent 41,2;1,5;1,6;1,7;z
H polyvalent 5Z4Z23+Z4724, Z4Z321Z 29,235+236.238H multivalent 6
Z3g+Z41Z429Z44
H multivalent 7
252Z53254255
H multivalent 8258#Z57,2609Z61,Z62
The final determination of each H antigen component should be based on the test results of H single factor serum. If there is no H single factor serum, two H composite factor sera should be used for verification.
If the first phase H antigen is detected but the second phase H antigen is not detected, or the second phase H antigen is detected but the first phase H antigen is not detected, it can be transferred to the agar slant for 1 to 2 generations before re-examination. If only one phase H antigen is still detected, the other phase should be checked by the phase variation method. Monophasic bacteria do not need to be tested for phase variation. The phase variation test method is as follows:
Small glass tube method: Dissolve the semisolid tube (about 1ml.~2mL per tube) on an alcohol lamp and cool to 50℃, take 0.05mL~0.1mL of H factor serum of known phase, add it to the dissolved semisolid, mix it, and use a capillary pipette to dispense it into the small glass tube for phase variation test. After solidification, use an inoculation needle to pick up the bacteria to be tested and inoculate it at one end. Place the small glass tube flat in a flat III, and put a ball of wet cotton next to it to prevent the water in the agar from evaporating and shrinking. Check the results every day. After the bacteria in the other phase are dissociated, bacteria can be picked from the other end for inspection. The concentration of serum in the culture medium should have an appropriate ratio. If it is too high, the bacteria cannot grow, and if it is too low, the power of bacteria in the same phase cannot be suppressed. Generally, it is added at a ratio of 1:200 to 1:800 of the original serum. Small inverted tube method: Place a small glass tube with both ends open (a gap should be left at the lower end, not flush) in a semi-solid tube. The upper end of the small glass tube should be higher than the surface of the culture medium. Sterilize and set aside. Heat and dissolve on an alcohol lamp before use, cool to 50°C, pick up a ring of factor serum, add it to the semi-solid in the small sleeve, stir it slightly to mix it, wait for solidification, inoculate the strain to be tested into the semi-solid surface in the small sleeve, check the results every day, wait for the other phase of bacteria to dissociate, take bacteria from the semi-solid surface outside the sleeve for inspection, or transfer to 1% soft agar slant, culture at 37°C and then do agglutination test.
Simple plate method: Dry the surface moisture of 0.7%-0.8% semi-solid agar plate, pick up a ring of factor serum, drop it on the surface of the semi-solid plate, leave it for a while, wait for the serum to be absorbed into the agar, and inoculate the strain to be tested in the center of the serum area. After culture, take bacteria from the edge of the spreading bacterial lawn for inspection.
6.4.4 Identification of Vi antigen
Use Vi factor serum to test. Known bacterial types with Vi antigen include: Salmonella typhi, Salmonella paratyphi C, and Salmonella dublin.
6.5 Determination of bacterial type and result report
Combining the results of the above biochemical tests and serological typing identification, determine the bacterial type according to Table 7 or the relevant Salmonella antigen table and report the results.
Salmonella Paratyphi A
Salmonella Kisangani
Salmonella Arechavarta
Salmonella Abortus
Salmonella Paratyphi B
Salmonella Limette
Salmonella Abonne
Salmonella Vienna
Salmonella Burry
Salmonella Stanley
Salmonella St. Paul
Salmonella Riddle
Salmonella Chester
Salmonella Deltro
Salmonella Agona
Salmonella Essen
Salmonella California
Salmonella Kingston
Salmonella Budapest
Salmonella typhimurium
Salmonella Lagush
Salmonella Bradenni
Salmonella Kilwa II
Salmonella Heidelberg
Salmonella Indiana
Salmonella Stanleyville
Salmonella Ituri
Salmonella Oslo
Salmonella Edinburgh
Salmonella Bloemfontein II
Salmonella Paratyphi C
S.paratyphiA
S.kisangani
S.arechavaleta
S.abortus-equi
S. paratyphi B
S. limete
S. taint- paut
S.reading
S.chester
S.california
S.kingston
S. budapest
S.typhimurium
S. bredeney
S.kitua n
S. heidelberg
S.indiana
S. stanleyville
S.edinburg
Common Salmonella antigen table
S.bloemfontein II
S.paratyphiC
1,21,2
1,4,[5],12
4,53,12
1,4,5,12
1,4,1 2,27
1,4,57,12,27
1,4,12,27
4,12,27
1,4,[57,12,27
1,4,53,12||t t||1,4,53,12
1,4,5),12
1,4,5],12
1,4,12
1,4,(57.12,27
1,4,12,2 7
1,4,53,12
1+4.53,12
1,4,12,27
1,4,15,12
1,4,12
1,45,12,27
1,4,12
6,7,cvi
Phase 1
Z4 +228
GB/T4789,4-2003
H antigen
Phase 2
[en,x]z
GB/T4789.4—2003
Salmonella choleraesuis
Salmonella typhisuis
Salmonella romitalis
Salmonella Brundenloops
Salmonella rissen
Salmonella Montevideo
Salmonella rigier Bacteria
Salmonella Orenburg
Salmonella Oritamanlin
Salmonella Thompson
Salmonella Concord
Salmonella Irumu
Salmonella Mkaba
Salmonella Bonn
Salmonella Potsdam
Salmonella Gdansk
Salmonella Virchow
Salmonella Infantis
Salmonella Papua
Salmonella Bareli Salmonella
Salmonella Hartford
Salmonella Mikawashima
Salmonella Mbandaka
Salmonella Tennessee
Salmonella Narashino
Salmonella Nagoya
Salmonella Galvani
Salmonella Munich
Salmonella FluorhardenbzxZ.net
Salmonella Newport
Salmonella Cottbus
Salmonella Zonwe
Lindenburg Salmonella
S.cholerae-suis
S,typhi-suis
S.braenderup
S.montevideo
S.oranienburg
S.oritamerin
S.thompson
S.concord
S.potsdam
S. rirchow
S.infantis
S.papuana
S. bareilly
S. hartford
S,mikawasima
S.mbandaka
S, tennessee
S. narashino
S,muenchen
S.manhattan
S. newport
S.kottbus
S.tshiongwe
S.lindenburg
Table 7 (continued)
0 antigen
Phase 1
H antigen
g,m,pl,s
Phase 2
e,n+215
[1,2,7]
e,n ,z15
e,n,zls
e,n,z15
Salmonella Takoradi
Salmonella Bonarene
Salmonella Litchfield
Salmonella Sick Bovine
Salmonella Charlie
Salmonella Baldo
Salmonella Imax
Salmonella Kentucky
Salmonella Brundenloop|| tt||14+ variants
Salmonella Jerusalem
Salmonella Sendai
Salmonella Typhi
Salmonella Tasi
Salmonella Eastbourne
Salmonella Israel
Salmonella Enteritidis
Salmonella Buridan
Salmonella II
Salmonella Dublin
Salmonella Hibiscus
Salmonella Panama
Salmonella Gottingen
Salmonella Ana Java
Salmonella Gallinarum
Salmonella Okefuko
Salmonella Waile
Salmonella Muenster
Salmonella Duck
Salmonella Newland
S, takoradi
S. bonariensis
S. litchfield
S.bovismorbificans
S.chailey
S.hentucky
S.braenderup
S.jerusalem
S.tarshyne
S.aastbourne
S. israel | |tt | gallinarum-pullorum
S.ckefoko
S. muenster
S.newlands
6,7,14
6,7,14
1,9,12
912,Vi
1,9,12||tt ||1,9,12
1,9,12
1,9,12,i
1,9,12
1,9,12
3,10153
Phase 1
Z4 ,223
GB/T4789.4—2003
H antigen
Phase 2
en,zis
esn,zis
gm,(s],t
[1,5][z】
GB/T4789.4—2003
Salmonella enterica
Salmonella regentis Salmonella
Salmonella Westhampton
Salmonella Armdelness
Salmonella New Rogoer
Salmonella Enluga
Salmonella New Stovf
Salmonella London
Salmonella Give
Salmonella Ruzizi
Salmonella Uganda
Salmonella Ugeli||tt| |Salmonella Wetevreden
Salmonella Clerkenwell
Salmonella Lexington
Salmonella Sarnos
Salmonella Calabar
Salmonella Sanftenberg
Salmonella Stellarfort||Salmonella Tuxony
Salmonella Thornburg
Salmonella Chandans
A Salmonella Bertin
Salmonella Briham
Salmonella Venezia
Salmonella Abatetuba
Salmonella Rubislau
Salmonella Poona
Salmonella Ritter
Salmonella Atlanta
Salmonella Mississippi
S. meleagridis
S. regent
S. tuesthampton
S.amounderness
S. neterochelle
S. mchanga
S. sinstorf
S,ughelli
S. ueltevreden
S. cderkenwell
S.lerington
S.calabar
S. senftenberg
S.sratford
S.taksony
S.schoeneberg
S,chandans
S.aberdee n
S.brijbhumi
S.reneziana
S.abaetetuba
S.rubislaw
S.atlanta
S.mississippi
Table 7 (continued)
0 antigen
3,10[15
3,1015
3, 1015
1,3,19
1,3,19
1,3,19
1,3,19
1,3,19
1,3,19
1,13,22
1,13,22
1,13,23
H antigen||tt ||Phase 1
Phase 2
24,723
e,n,zis
esn+zis
e+n,2/s
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