Some standard content:
National Metrology Verification Regulation of the People's Republic of China JJG536-98
Polarimeter and Saccharimeter
Approved on July 14, 1998
Implemented on January 15, 1999
State Administration of Quality and Technical Supervision
Verification Regulation .of
Polarimeter and SaccharimeterJJG 536—98
Replaces JJG536—88
JJG675-
This verification procedure was approved by the State Administration of Quality and Technical Supervision on July 14, 1998, and came into effect on January 15, 1999.
Responsible unit: National Technical Committee for Physical and Chemical Metrology Drafting unit: Heilongjiang Provincial Metrology Verification and Testing Institute The drafting unit is responsible for interpreting the technical provisions of this procedure. Main drafters of this regulation:
Gu Zhiming
Ding Haiming
Participating drafters:
(Heilongjiang Provincial Metrology Verification and Testing Institute)
(Heilongjiang Provincial Metrology Verification and Testing Institute)
(Heilongjiang Provincial Metrology Verification and Testing Institute)
(Heilongjiang Provincial Metrology Verification and Testing Institute)
Technical requirements
Verification conditions
Verification items and verification methods
Verification result processing and verification cycle
Appendix 1 ICUMSA International Sugar Scale
Appendix 2 Verification record format
Appendix 3 Verification certificate and verification result notification format (back) 12
(2)
(6)
(7)
JJG 536—98
Total 20 pages Page 1
Verification procedures for polarimeters and polarimeter sugar meters
This procedure is applicable to the verification of 0.02, 0.05 visual polarimeters, 0.1, 0.2 visual polarimeter sugar meters, 0.01, 0.02, 0.05 automatic polarimeters, 0.05, 0.1, 0.2 automatic polarimeter sugar meters, newly manufactured, in use and after repair. I. Overview
Polarimeter is an instrument for measuring the optical rotation of a substance. The instrument is divided into two categories: visual polarimeter and automatic polarimeter according to the working principle of the instrument. The polarimeter is a polarimeter based on the international sugar scale. According to the working principle of the instrument, it is divided into two categories: visual polarimeter and automatic polarimeter. Through the determination of optical rotation or sugar content, the concentration, purity, sugar content, etc. of the substance can be analyzed. It is widely used in production, inspection and scientific research departments such as pharmaceuticals, sugar making, petroleum, food, chemical industry, and medical treatment. The optical rotation of optically active substances is related to the wavelength of the light source, the optical path length of the optically active substance, The optical rotation of a solution at a certain wavelength and temperature is:
Where: α - optical rotation, unit is degree (°) (1)
C - concentration of the optically active substance solution, unit is gram/milliliter (g/ml): L - length of the liquid column filled with the optically active substance solution, unit is decimeter (dm). If C=, L=, then α=, which is called specific optical rotation. By measuring the optical rotation α, it can be based on The concentration of the substance being measured is calculated using the following formula: C
The working principle of the polarimeter and polarimeter is: a parallel beam of monochromatic light is generated by a light source, condenser, aperture, filter, etc., and natural light is converted into polarized light through a polarizer, and then is emitted to the eyepiece (visual instrument) or photomultiplier tube (automatic instrument) through a test tube and an analyzer. When the instrument is at optical zero, the vibration planes of the polarizer and the analyzer are perpendicular to each other, and are basically opaque to light. The eyepiece field of view is a dark field or the photomultiplier tube has no output. When an optically active substance is put into the test tube, the optically active substance rotates the polarized light by a certain angle, so that the incident light is not perpendicular to the vibration plane of the analyzer, thereby generating a certain intensity of transmitted light, the eyepiece field of view is a bright field of view or the photomultiplier tube has an output, and then the analyzer (or polarizer, quartz wedge) connected to the dial is rotated manually or by a servo motor to reach the optical equilibrium point that is basically opaque, so that the optical rotation or sugar content can be read or the instrument displays it. Figure 1 is the optical structure diagram of a visual polarimeter and a visual optical polarimeter. Figures 2 and 3 are working principle diagrams of common automatic polarimeters and automatic polarimeter sugar meters. 15
1—light source;
2--ground glass;
3-condenser:
4-filter:
5polarizer;
optical structure diagram of visual polarimeter
6 half-wave plate;
7-test tube:
8-analyzer;
9objective lens group,
10 one-degree disk rotation hand wheel:
11--focusing hand wheel;
|12--Reading magnifying glass;
13--Dial and vernier;
14--Protective glass:
15--Eyepiece set
II Technical requirements
(--) Visual polarimeter and otic polarimeter 1 The basic parameters of the visual polarimeter and visual polarimeter and the sensitivity threshold and indication error of the instrument shall comply with the provisions of Table 1
2 The appearance of the instrument shall comply with the following requirements
2.1 The instrument shall have complete markings (name, model, number, manufacturer and date of manufacture) 536—98
Figure 2 Automatic polarimeter with dial indication
Total 20 pages
Page 3
1—light source; 2—aperture: 3—condenser; 4—polarizer; 5—dial: 6—Faraday modulator; 7—test tube; 8—filter; 9—analyzer; 10—photomultiplier tube; 11—amplifier 12—servo motor; 13—worm gear Table 1
Basic parameters and some verification items of visual polarimeter and visual polarimeter Instrument level
Minimum graduation value
Sensitivity threshold
Average indication Error
Visual polarimeter
-180°
+180°
≤0.02°
≤0.05°
+180°
Visual polarimeter
(20°2-
+105°z)
≤0.1°z
2(-20°Z-
+105°2)
2.2 The exposed surface of optical parts shall not have cracks, scratches, oil stains, mildew, etc. Total 20 pages
Page 4
JJG 536-98
Figure 3 Automatic polarimeter with quartz wedge compensator 1- self-incandescent lamp; 2- filter; 3- polarizer; 4- quartz wedge compensator; 5- test tube; 6- Faraday modulator; 7- analyzer; 8- photomultiplier tube; 9- amplifier; 10- servo motor; 11- counting and reading device 2.3 All engraved lines and inscriptions should be clear and even, and there should be no rust, glare and other phenomena that hinder reading and measurement.
3 All movable parts of the instrument should be able to level Rotate steadily without any stagnation or sudden jump. 4 The field of view of the instrument eyepiece should be circular after focusing, with a clear outline, clean field of view, and clear boundary lines. When the instrument is at optical zero, the boundary lines disappear, and the brightness and color of the field of view are consistent. (II) Automatic polarimeter and automatic polarimeter sugar meter 5 The basic parameters of the automatic polarimeter and automatic polarimeter sugar meter and the indication error, repeatability and stability of the instrument should comply with the provisions of Table 2. 6 The temperature rise in the test box of the automatic polarimeter sugar meter should not exceed 1 ℃.
JJG 536—98
7 The measuring time of the automatic polarimeter should not exceed 308.8 Appearance inspection and preliminary test,
Total 20 pages Page 5
Table 2 Basic parameters and some verification items of automatic polarimeter and automatic polarimeter Instrument
Minimum graduation value
Measuring range
Indication error
Repeatability
Stability
≥(-45°
Automatic polarimeter
≤0.005°
≥( 45 ° |
≥(-20
+105°z)
003°z
≤0.1°Z
≤0.05°z
≥(-20°z
～+105)
≤0.07°z
≤0.2°z
8.1 The instrument should have complete markings (name, model, factory number, manufacturer and date of manufacture, etc.).
8.2 All fasteners should be well tightened, all adjustment knobs, buttons and switches should work normally, the servo motor should work smoothly without obvious noise, the test box should be easy to open and close, and the exit and entrance window glass should be clean and free of scratches. 8.3 The light source lamp should glow and emit light normally, and its beam should not have jitter and flashing. 8.4 The dial should be clean and bright, the engraved lines or displayed numbers should be clear, and the engraved lines to be aligned should be parallel.
(III) Test tube
9 The cover glass on the test tube shall not have cracks, scratches or gaps. 10 Empty test tube test
When an empty test tube with a nut but without a cover glass is placed in the test light path and rotated along its tube axis: for visual instruments, the brightness balance of the field of view when there is no empty tube should not be destroyed; for automatic instruments, there should be no zero point offset when there is no empty tube. 11 Check for stress in the cover glass: When a cover glass installed on an empty test tube is relatively When the other piece rotates around the tube axis between 0° and 180°, and the empty test tube together with the cover glass rotates between 0° and 180° (or when such rotation is not possible, the two cover glasses are rotated simultaneously relative to the test tube), the instrument indication change shall not be greater than the minimum graduation value. 12 The error in the length of the test tube, at 20°C, the indication error shall not be greater than 1/3 of the length tolerance in the second column of Table 3 The measuring tool or meter is calibrated, and the measurement is made at 4 equally divided points on the circumference of the two end faces. The difference between the average value and the nominal length should comply with the provisions of Table 3 and Table 3
Nominal length
Requirements for test tube length
Tolerance between actual length and nominal length
(0.01%)
Test tube products should be marked with the manufacturer or factory name and the actual length value with an accuracy level of 0.01% or a level of accuracy of 0.2%. The verification results give the actual length value with an accuracy level of 0.01% or 0.2%.
Three verification conditions
13 Verification environment conditions
13.1 Indoor temperature (15~30)℃, relative humidity ≤85%13.2 Power supply voltage (220±22)V, frequency (50±1)Hz13.3 The workbench should be stable, without obvious impact and vibration, and without strong electromagnetic interference.
13.4 The room for visual instrument verification should be shielded from light, or carried out in a dark room or semi-dark room so that the eyes can adapt to the dark field well.
14 Verification equipment and instruments are shown in Table 4.
JJG 536—98
Serial number name
Calibration equipment and instruments
(1) Optical rotation, sugar content (6 kinds in total, at a wavelength of 589.4400mm):
+5.194°(+15Z), 5.194°(-15z) Standard rotation
Length measurement
Tool or measurementwwW.bzxz.Net
+17.313(+50°Z), -17.313°(-50°2)+34.626(+100°Z), -34.626(-100°Z) (2 Direction error*: not more than 0.003
(±0.01°z)
(3) Expanded uncertainty*￥(k=2): ≤0.004%(0. 01°Z)
(4) Range of value variation: not more than 1° (±3°Z) Digital thermometer (probe can be attached): (1) Measuring range: not less than (15℃~40℃) (2) Indication error: not more than ±0.2℃
(1) Measuring range: not less than (50nm~200nm) (2 Indication error: not more than 1/3 of the difference in Table 3 220V.0.5kVA, stability 0.5%
Total 20 pages Page 7
(1) Standard polarimeter calibration certificate, should be given
58 at 20℃ The optical rotation and sugar content at 9.4400nm and 546.2271nm wavelengths and the sugar content at 587.0000mm wavelength should also be given. The expanded uncertainty and directional error should also be given. (2) The directional error of the two branches of +5°C and -5°C is allowed to be no more than
. It is recommended to use a specially designed thermistor.
Digital thermometer
No need to specially configure
No need to specially configure
No need to specially configure
* Direction error refers to The maximum difference between the calibration value at a wavelength of 589.4400m and the displayed value when the calibrated polarimeter rotates along the tube axis.
** The calculation of the expanded uncertainty should include the direction error. IV. Verification Items and Verification Methods
(-I) Verification of Otoscope Polarimeter and Visual Polarimeter 15. Item 24. Verification by visual observation or manual test according to the requirements of each article.
16. Verification of Instrument Sensitivity
After the instrument is lit according to the use requirements and the light source is normal, first adjust to find the zero point of the brightness balance of the two-thirds or three-thirds field of view, and then adjust the dial from Rotate the zero position left or right (3 times each) to adjust the sensitivity tolerance, and then observe whether there is any perceptible change in the brightness balance of the two-thirds or three-thirds field of view. If so, it means that the instrument is qualified. For the slow sensitivity verification, just read one side of the scale values on the left and right sides of the dial. A total of 20 pages, page 8
JJG536---98
If the sensitivity reading is unqualified, it can be re-debugged or the light source lamp can be replaced and then verified again. 17 Verification of instrument indication error
Each reading in this verification, if there are two reading windows, refers to the average of the two readings on the left and right sides of the dial.
First, measure the instrument zero value when the empty test tube is closed, rotate the dial clockwise and counterclockwise in turn to achieve field brightness balance, write down the dial reading, and repeat this process. Take the average of 6 readings as the instrument zero value. Put the standard polarimeter into the test tube and position it in the middle (the instrument with the test tube set at an angle needs to be fixed with plasticine), stick the probe of the digital thermometer on the body of the standard polarimeter close to the quartz plate with white tape, and connect the digital thermometer. Close the test tube. Depending on the temperature difference between the tube and the standard polarimeter, the standard polarimeter needs to be kept still in the tube for (7-10) minutes to allow the temperature of the standard polarimeter to reach equilibrium with that of the test tube. When measuring, rotate the dial clockwise and counterclockwise in turn. When the brightness of the field of view is consistent, record the temperature value and the dial reading in turn. Repeat this measurement 6 times. The dial reading minus the instrument zero value is the instrument measurement value. Next, according to the temperature measured each time, calculate the standard value of the optical rotation or sugar content of the standard polarimeter t℃ using the following formula: α-a201+0.000144(t-20)1
Where: α is the optical rotation or sugar content of the standard polarimeter at the measuring temperature; α20℃ is the optical rotation or sugar content of the standard polarimeter at 20℃; t is the temperature of the standard polarimeter during measurement, (3)
The average value of the difference between the instrument's measured value and the standard value (indication error) shall comply with the indication error verification specified in Table 1. According to the instrument's measuring range, the indication error verification readings are taken at +5° and -5° (+15°Z and -15\Z), +17Z and 17°Z (+50Z and -50), +35\Z and -35Z (+100°Z and -100°Z). It is required that the difference between the maximum and minimum values of the six values measured at each point shall not exceed the sensitivity tolerance. When the reading does not meet the requirements, it shall be measured and read again. (II) Verification of automatic polarimeters and automatic polarimeter sugar meters 18 Article 8 Verification shall be carried out by visual observation and manual test as required. 19 Verification of instrument indication error and repeatability According to the instrument's measuring range, the verification of instrument indication error and repeatability shall be carried out by using six standard4 The dial should be clean and bright, the scale lines or displayed numbers should be clear, and the scale lines to be aligned should be parallel.
(III) Test tubes
9 The cover glass on the test tube should not have cracks, scratches or gaps 10 Empty test tube test
When an empty test tube with a nut but no cover glass is placed in the test light path and rotated along its tube axis: for visual instruments, the field brightness balance when there is no empty tube should not be destroyed; for automatic instruments, there should be no zero point offset when there is no empty tube 11 Check for stress in the cover glass: When a cover glass installed on an empty test tube is relatively When the other piece rotates around the tube axis between 0° and 180°, and the empty test tube together with the cover glass rotates between 0° and 180° (or when such rotation is not possible, the two cover glasses are rotated simultaneously relative to the test tube), the instrument indication change shall not be greater than the minimum graduation value. 12 The error in the length of the test tube, at 20°C, the indication error shall not be greater than 1/3 of the length tolerance in the second column of Table 3 The measuring tool or meter is calibrated, and the measurement is made at 4 equally divided points on the circumference of the two end faces. The difference between the average value and the nominal length should comply with the provisions of Table 3 and Table 3
Nominal length
Requirements for test tube length
Tolerance between actual length and nominal length
(0.01%)
Test tube products should be marked with the manufacturer or factory name and the actual length value with an accuracy level of 0.01% or a level of accuracy of 0.2%. The verification results give the actual length value with an accuracy level of 0.01% or 0.2%.
Three verification conditions
13 Verification environment conditions
13.1 Indoor temperature (15~30)℃, relative humidity ≤85%13.2 Power supply voltage (220±22)V, frequency (50±1)Hz13.3 The workbench should be stable, without obvious impact and vibration, and without strong electromagnetic interference.
13.4 The room for visual instrument verification should be shielded from light, or carried out in a dark room or semi-dark room so that the eyes can adapt to the dark field well.
14 Verification equipment and instruments are shown in Table 4.
JJG 536—98
Serial number name
Calibration equipment and instruments
(1) Optical rotation, sugar content (6 kinds in total, at a wavelength of 589.4400mm):
+5.194°(+15Z), 5.194°(-15z) Standard rotation
Length measurement
Tool or measurement
+17.313(+50°Z), -17.313°(-50°2)+34.626(+100°Z), -34.626(-100°Z) (2 Direction error*: not more than 0.003
(±0.01°z)
(3) Expanded uncertainty*￥(k=2): ≤0.004%(0. 01°Z)
(4) Range of value variation: not more than 1° (±3°Z) Digital thermometer (probe can be attached): (1) Measuring range: not less than (15℃~40℃) (2) Indication error: not more than ±0.2℃
(1) Measuring range: not less than (50nm~200nm) (2 Indication error: not more than 1/3 of the difference in Table 3 220V.0.5kVA, stability 0.5%
Total 20 pages Page 7
(1) Standard polarimeter calibration certificate, should be given
58 at 20℃ The optical rotation and sugar content at 9.4400nm and 546.2271nm wavelengths and the sugar content at 587.0000mm wavelength should also be given. The expanded uncertainty and directional error should also be given. (2) The directional error of the two branches of +5°C and -5°C is allowed to be no more than
. It is recommended to use a specially designed thermistor.
Digital thermometer
No need to specially configure
No need to specially configure
No need to specially configure
* Direction error refers to The maximum difference between the calibration value at a wavelength of 589.4400m and the displayed value when the calibrated polarimeter rotates along the tube axis.
** The calculation of the expanded uncertainty should include the direction error. IV. Verification Items and Verification Methods
(-I) Verification of Otoscope Polarimeter and Visual Polarimeter 15. Item 24. Verification by visual observation or manual test according to the requirements of each article.
16. Verification of Instrument Sensitivity
After the instrument is lit according to the use requirements and the light source is normal, first adjust to find the zero point of the brightness balance of the two-thirds or three-thirds field of view, and then adjust the dial from Rotate the zero position left or right (3 times each) to adjust the sensitivity tolerance, and then observe whether there is any perceptible change in the brightness balance of the two-thirds or three-thirds field of view. If so, it means that the instrument is qualified. For the slow sensitivity verification, just read one side of the scale values on the left and right sides of the dial. A total of 20 pages, page 8
JJG536---98
If the sensitivity reading is unqualified, it can be re-debugged or the light source lamp can be replaced and then verified again. 17 Verification of instrument indication error
Each reading in this verification, if there are two reading windows, refers to the average of the two readings on the left and right sides of the dial.
First, measure the instrument zero value when the empty test tube is closed, rotate the dial clockwise and counterclockwise in turn to achieve field brightness balance, write down the dial reading, and repeat this process. Take the average of 6 readings as the instrument zero value. Put the standard polarimeter into the test tube and position it in the middle (the instrument with the test tube set at an angle needs to be fixed with plasticine), stick the probe of the digital thermometer on the body of the standard polarimeter close to the quartz plate with white tape, and connect the digital thermometer. Close the test tube. Depending on the temperature difference between the tube and the standard polarimeter, the standard polarimeter needs to be kept still in the tube for (7-10) minutes to allow the temperature of the standard polarimeter to reach equilibrium with that of the test tube. When measuring, rotate the dial clockwise and counterclockwise in turn. When the brightness of the field of view is consistent, record the temperature value and the dial reading in turn. Repeat this measurement 6 times. The dial reading minus the instrument zero value is the instrument measurement value. Next, according to the temperature measured each time, calculate the standard value of the optical rotation or sugar content of the standard polarimeter t℃ using the following formula: α-a201+0.000144(t-20)1
Where: α is the optical rotation or sugar content of the standard polarimeter at the measuring temperature; α20℃ is the optical rotation or sugar content of the standard polarimeter at 20℃; t is the temperature of the standard polarimeter during measurement, (3)
The average value of the difference between the instrument's measured value and the standard value (indication error) shall comply with the indication error verification specified in Table 1. According to the instrument's measuring range, the indication error verification readings are taken at +5° and -5° (+15°Z and -15\Z), +17Z and 17°Z (+50Z and -50), +35\Z and -35Z (+100°Z and -100°Z). It is required that the difference between the maximum and minimum values of the six values measured at each point shall not exceed the sensitivity tolerance. When the reading does not meet the requirements, it shall be measured and read again. (II) Verification of automatic polarimeters and automatic polarimeter sugar meters 18 Article 8 Verification shall be carried out by visual observation and manual test as required. 19 Verification of instrument indication error and repeatability According to the instrument's measuring range, the verification of instrument indication error and repeatability shall be carried out by using six standard4 The dial should be clean and bright, the scale lines or displayed numbers should be clear, and the scale lines to be aligned should be parallel.
(III) Test tubes
9 The cover glass on the test tube should not have cracks, scratches or gaps 10 Empty test tube test
When an empty test tube with a nut but no cover glass is placed in the test light path and rotated along its tube axis: for visual instruments, the field brightness balance when there is no empty tube should not be destroyed; for automatic instruments, there should be no zero point offset when there is no empty tube 11 Check for stress in the cover glass: When a cover glass installed on an empty test tube is relatively When the other piece rotates around the tube axis between 0° and 180°, and the empty test tube together with the cover glass rotates between 0° and 180° (or when such rotation is not possible, the two cover glasses are rotated simultaneously relative to the test tube), the instrument indication change shall not be greater than the minimum graduation value. 12 The error in the length of the test tube, at 20°C, the indication error shall not be greater than 1/3 of the length tolerance in the second column of Table 3 The measuring tool or meter is calibrated, and the measurement is made at 4 equally divided points on the circumference of the two end faces. The difference between the average value and the nominal length should comply with the provisions of Table 3 and Table 3
Nominal length
Requirements for test tube length
Tolerance between actual length and nominal length
(0.01%)
Test tube products should be marked with the manufacturer or factory name and the actual length value with an accuracy level of 0.01% or a level of accuracy of 0.2%. The verification results give the actual length value with an accuracy level of 0.01% or 0.2%.
Three verification conditions
13 Verification environment conditions
13.1 Indoor temperature (15~30)℃, relative humidity ≤85%13.2 Power supply voltage (220±22)V, frequency (50±1)Hz13.3 The workbench should be stable, without obvious impact and vibration, and without strong electromagnetic interference.
13.4 The room for visual instrument verification should be shielded from light, or carried out in a dark room or semi-dark room so that the eyes can adapt to the dark field well.
14 Verification equipment and instruments are shown in Table 4.
JJG 536—98
Serial number name
Calibration equipment and instruments
(1) Optical rotation, sugar content (6 kinds in total, at a wavelength of 589.4400mm):
+5.194°(+15Z), 5.194°(-15z) Standard rotation
Length measurement
Tool or measurement
+17.313(+50°Z), -17.313°(-50°2)+34.626(+100°Z), -34.626(-100°Z) (2 Direction error*: not more than 0.003
(±0.01°z)
(3) Expanded uncertainty*￥(k=2): ≤0.004%(0. 01°Z)
(4) Range of value variation: not more than 1° (±3°Z) Digital thermometer (probe can be attached): (1) Measuring range: not less than (15℃~40℃) (2) Indication error: not more than ±0.2℃
(1) Measuring range: not less than (50nm~200nm) (2 Indication error: not more than 1/3 of the difference in Table 3 220V.0.5kVA, stability 0.5%
Total 20 pages Page 7
(1) Standard polarimeter calibration certificate, should be given
58 at 20℃ The optical rotation and sugar content at 9.4400nm and 546.2271nm wavelengths and the sugar content at 587.0000mm wavelength should also be given. The expanded uncertainty and directional error should also be given. (2) The directional error of the two branches of +5°C and -5°C is allowed to be no more than
. It is recommended to use a specially designed thermistor.
Digital thermometer
No need to specially configure
No need to specially configure
No need to specially configure
* Direction error refers to The maximum difference between the calibration value at a wavelength of 589.4400m and the displayed value when the calibrated polarimeter rotates along the tube axis.
** The calculation of the expanded uncertainty should include the direction error. IV. Verification Items and Verification Methods
(-I) Verification of Otoscope Polarimeter and Visual Polarimeter 15. Item 24. Verification by visual observation or manual test according to the requirements of each article.
16. Verification of Instrument Sensitivity
After the instrument is lit according to the use requirements and the light source is normal, first adjust to find the zero point of the brightness balance of the two-thirds or three-thirds field of view, and then adjust the dial from Rotate the zero position left or right (3 times each) to adjust the sensitivity tolerance, and then observe whether there is any perceptible change in the brightness balance of the two-thirds or three-thirds field of view. If so, it means that the instrument is qualified. For the slow sensitivity verification, just read one side of the scale values on the left and right sides of the dial. A total of 20 pages, page 8
JJG536---98
If the sensitivity reading is unqualified, it can be re-debugged or the light source lamp can be replaced and then verified again. 17 Verification of instrument indication error
Each reading in this verification, if there are two reading windows, refers to the average of the two readings on the left and right sides of the dial.
First, measure the instrument zero value when the empty test tube is closed, rotate the dial clockwise and counterclockwise in turn to achieve field brightness balance, write down the dial reading, and repeat this process. Take the average of 6 readings as the instrument zero value. Put the standard polarimeter into the test tube and position it in the middle (the instrument with the test tube set at an angle needs to be fixed with plasticine), stick the probe of the digital thermometer on the body of the standard polarimeter close to the quartz plate with white tape, and connect the digital thermometer. Close the test tube. Depending on the temperature difference between the tube and the standard polarimeter, the standard polarimeter needs to be kept still in the tube for (7-10) minutes to allow the temperature of the standard polarimeter to reach equilibrium with that of the test tube. When measuring, rotate the dial clockwise and counterclockwise in turn. When the brightness of the field of view is consistent, record the temperature value and the dial reading in turn. Repeat this measurement 6 times. The dial reading minus the instrument zero value is the instrument measurement value. Next, according to the temperature measured each time, calculate the standard value of the optical rotation or sugar content of the standard polarimeter t℃ using the following formula: α-a201+0.000144(t-20)1
Where: α is the optical rotation or sugar content of the standard polarimeter at the measuring temperature; α20℃ is the optical rotation or sugar content of the standard polarimeter at 20℃; t is the temperature of the standard polarimeter during measurement, (3)
The average value of the difference between the instrument's measured value and the standard value (indication error) shall comply with the indication error verification specified in Table 1. According to the instrument's measuring range, the indication error verification readings are taken at +5° and -5° (+15°Z and -15\Z), +17Z and 17°Z (+50Z and -50), +35\Z and -35Z (+100°Z and -100°Z). It is required that the difference between the maximum and minimum values of the six values measured at each point shall not exceed the sensitivity tolerance. When the reading does not meet the requirements, it shall be measured and read again. (II) Verification of automatic polarimeters and automatic polarimeter sugar meters 18 Article 8 Verification shall be carried out by visual observation and manual test as required. 19 Verification of instrument indication error and repeatability According to the instrument's measuring range, the verification of instrument indication error and repeatability shall be carried out by using six standard4400mm wavelength):
+5.194°(+15Z),5.194°(-15z) standard rotation
length measurement
tool or measurement
+17.313(+50°Z),-17.313°(-50°2)+34.626(+100°Z),-34.626(-100°Z) (2 direction error*: not more than 0.003
(±0.01°z)
(3) expanded uncertainty*￥(k=2): ≤0.004%(0. 01°Z)
(4) Range of value variation: not more than 1° (±3°Z) Digital thermometer (probe can be attached): (1) Measuring range: not less than (15℃~40℃) (2) Indication error: not more than ±0.2℃
(1) Measuring range: not less than (50nm~200nm) (2 Indication error: not more than 1/3 of the difference in Table 3 220V.0.5kVA, stability 0.5%
Total 20 pages Page 7
(1) The calibration certificate of the standard polarimeter should give the optical rotation and sugar content at wavelengths of 589.4400nm and 546.2271nm at 20℃ The sugar content value and the sugar content value at a wavelength of 587.0000mm
, and the expanded uncertainty and directional error should be given
(2) The directional error of the two branches at +5°℃ and -5°℃ is allowed to be no more than
It is recommended to use a specially designed thermistor
Digital thermometer
No need to be specially configured
No need to be specially configured
No need to be specially configured
* The directional error refers to the maximum difference between the calibrated value at a wavelength of 589.4400m and the displayed value when the calibrated polarimeter rotates along the tube axis.
** The calculation of the expanded uncertainty should include the directional error. IV. Verification Items and Verification Methods
(-I) Verification of Otological Polarimeter and Visual Polarimeter 15. Item 24. Verification by visual observation or manual test according to the requirements of each article.
16 Verification of Instrument Sensitivity
After the instrument is lit according to the use requirements and the light source is normal, first adjust to find the zero point of the brightness balance of the two-thirds or three-thirds field of view, then rotate the dial left or right (3 times each) from the zero point position to the degree of sensitivity tolerance, and then observe whether there is a perceptible change in the brightness balance of the two-thirds or three-thirds field of view. If there is, it means that the instrument is qualified. For slow sensitivity verification, just read one side of the scale value on the left and right sides of the dial. Total 20 pages Page 8
JJG536---98
Sensitivity reading Unqualified instruments can be re-debugged or replaced with light sources and then recalibrated. 17 Verification of instrument indication error
Each reading in this verification, if there are two reading windows, refers to the average of the two readings on the left and right of the dial.
First, measure the instrument zero value when the empty test tube is closed, rotate the dial clockwise and counterclockwise in turn to achieve field brightness balance, record the dial reading, repeat this process, and take the average of 6 readings as the instrument zero value. Put the standard polarimeter into the test tube and place it in the middle (the instrument with the test tube set at an angle needs to be fixed with plasticine), stick the probe of the digital thermometer on the body of the standard polarimeter tube close to the quartz plate with white tape, and connect the digital thermometer. Close the test tube. Depending on the temperature difference between the tube and the standard polarimeter, the standard polarimeter needs to be kept still in the tube for (7-10) minutes to allow the temperature of the standard polarimeter to reach equilibrium with that of the test tube. When measuring, rotate the dial clockwise and counterclockwise in turn. When the brightness of the field of view is consistent, record the temperature value and the dial reading in turn. Repeat this measurement 6 times. The dial reading minus the instrument zero value is the instrument measurement value. Next, according to the temperature measured each time, calculate the standard value of the optical rotation or sugar content of the standard polarimeter t℃ using the following formula: α-a201+0.000144(t-20)1
Where: α is the optical rotation or sugar content of the standard polarimeter at the measuring temperature; α20℃ is the optical rotation or sugar content of the standard polarimeter at 20℃; t is the temperature of the standard polarimeter during measurement, (3)
The average value of the difference between the instrument's measured value and the standard value (indication error) shall comply with the indication error verification specified in Table 1. According to the instrument's measuring range, the indication error verification readings are taken at +5° and -5° (+15°Z and -15\Z), +17Z and 17°Z (+50Z and -50), +35\Z and -35Z (+100°Z and -100°Z). It is required that the difference between the maximum and minimum values of the six values measured at each point shall not exceed the sensitivity tolerance. When the reading does not meet the requirements, it shall be measured and read again. (II) Verification of automatic polarimeters and automatic polarimeter sugar meters 18 Article 8 Verification shall be carried out by visual observation and manual test as required. 19 Verification of instrument indication error and repeatability According to the instrument's measuring range, the verification of instrument indication error and repeatability shall be carried out by using six standard4400mm wavelength):
+5.194°(+15Z),5.194°(-15z) standard rotation
length measurement
tool or measurement
+17.313(+50°Z),-17.313°(-50°2)+34.626(+100°Z),-34.626(-100°Z) (2 direction error*: not more than 0.003
(±0.01°z)
(3) expanded uncertainty*￥(k=2): ≤0.004%(0. 01°Z)
(4) Range of value variation: not more than 1° (±3°Z) Digital thermometer (probe can be attached): (1) Measuring range: not less than (15℃~40℃) (2) Indication error: not more than ±0.2℃
(1) Measuring range: not less than (50nm~200nm) (2 Indication error: not more than 1/3 of the difference in Table 3 220V.0.5kVA, stability 0.5%
Total 20 pages Page 7
(1) The calibration certificate of the standard polarimeter should give the optical rotation and sugar content at wavelengths of 589.4400nm and 546.2271nm at 20℃ The sugar content value and the sugar content value at a wavelength of 587.0000mm
, and the expanded uncertainty and directional error should be given
(2) The directional error of the two branches at +5°℃ and -5°℃ is allowed to be no more than
It is recommended to use a specially designed thermistor
Digital thermometer
No need to be specially configured
No need to be specially configured
No need to be specially configured
* The directional error refers to the maximum difference between the calibrated value at a wavelength of 589.4400m and the displayed value when the calibrated polarimeter rotates along the tube axis.
** The calculation of the expanded uncertainty should include the directional error. IV. Verification Items and Verification Methods
(-I) Verification of Otological Polarimeter and Visual Polarimeter 15. Item 24. Verification by visual observation or manual test according to the requirements of each article.
16 Verification of Instrument Sensitivity
After the instrument is lit according to the use requirements and the light source is normal, first adjust to find the zero point of the brightness balance of the two-thirds or three-thirds field of view, then rotate the dial left or right (3 times each) from the zero point position to the degree of sensitivity tolerance, and then observe whether there is a perceptible change in the brightness balance of the two-thirds or three-thirds field of view. If there is, it means that the instrument is qualified. For slow sensitivity verification, just read one side of the scale value on the left and right sides of the dial. Total 20 pages Page 8
JJG536---98
Sensitivity reading Unqualified instruments can be re-debugged or replaced with light sources and then recalibrated. 17 Verification of instrument indication error
Each reading in this verification, if there are two reading windows, refers to the average of the two readings on the left and right of the dial.
First, measure the instrument zero value when the empty test tube is closed, rotate the dial clockwise and counterclockwise in turn to achieve field brightness balance, record the dial reading, repeat this process, and take the average of 6 readings as the instrument zero value. Put the standard polarimeter into the test tube and place it in the middle (the instrument with the test tube set at an angle needs to be fixed with plasticine), stick the probe of the digital thermometer on the body of the standard polarimeter tube close to the quartz plate with white tape, and connect the digital thermometer. Close the test tube. Depending on the temperature difference between the tube and the standard polarimeter, the standard polarimeter needs to be kept still in the tube for (7-10) minutes to allow the temperature of the standard polarimeter to reach equilibrium with that of the test tube. When measuring, rotate the dial clockwise and counterclockwise in turn. When the brightness of the field of view is consistent, record the temperature value and the dial reading in turn. Repeat this measurement 6 times. The dial reading minus the instrument zero value is the instrument measurement value. Next, according to the temperature measured each time, calculate the standard value of the optical rotation or sugar content of the standard polarimeter t℃ using the following formula: α-a201+0.000144(t-20)1
Where: α is the optical rotation or sugar content of the standard polarimeter at the measuring temperature; α20℃ is the optical rotation or sugar content of the standard polarimeter at 20℃; t is the temperature of the standard polarimeter during measurement, (3)
The average value of the difference between the instrument's measured value and the standard value (indication error) shall comply with the indication error verification specified in Table 1. According to the instrument's measuring range, the indication error verification readings are taken at +5° and -5° (+15°Z and -15\Z), +17Z and 17°Z (+50Z and -50), +35\Z and -35Z (+100°Z and -100°Z). It is required that the difference between the maximum and minimum values of the six values measured at each point shall not exceed the sensitivity tolerance. When the reading does not meet the requirements, it shall be measured and read again. (II) Verification of automatic polarimeters and automatic polarimeter sugar meters 18 Article 8 Verification shall be carried out by visual observation and manual test as required. 19 Verification of instrument indication error and repeatability According to the instrument's measuring range, the verification of instrument indication error and repeatability shall be carried out by using six standardThe maximum difference between the calibration value at 4400m wavelength and the displayed value when the standard polarimeter rotates along the tube axis,
**The calculation of expanded uncertainty should include the direction error, IV. Verification items and verification methods
(-I) Verification of otoscope polarimeters and visual polarimeter sugar meters 15. Item 24 Verification by visual observation or manual test according to the requirements of each article,
16 Verification of instrument sensitivity
After the instrument is lit according to the use requirements and the light source is normal, first adjust to find the zero point of the brightness balance of the two-thirds or three-thirds field of view, and then adjust the dial from the zero point Rotate the position left or right (3 times each) to the degree of sensitivity tolerance, and then observe whether there is a noticeable change in the brightness balance of the two-thirds or three-thirds field of view. If so, it means that the instrument recording sensitivity is qualified. For the slow sensitivity verification, just read one side of the scale values on the left and right sides of the dial. A total of 20 pages, page 8
JJG536---98
If the sensitivity reading is unqualified, it can be re-debugged or the light source lamp can be replaced and then verified again. 17 Verification of instrument indication error
Each reading in this verification, if there are two reading windows, refers to the average of the two readings on the left and right sides of the dial.
First, measure the instrument zero value when the empty test tube is closed, rotate the dial clockwise and counterclockwise in turn to achieve field brightness balance, write down the dial reading, and repeat this process. Take the average of 6 readings as the instrument zero value. Put the standard polarimeter into the test tube and position it in the middle (the instrument with the test tube set at an angle needs to be fixed with plasticine), stick the probe of the digital thermometer on the body of the standard polarimeter close to the quartz plate with white tape, and connect the digital thermometer. Close the test tube. Depending on the temperature difference between the tube and the standard polarimeter, the standard polarimeter needs to be kept still in the tube for (7-10) minutes to allow the temperature of the standard polarimeter to reach equilibrium with that of the test tube. When measuring, rotate the dial clockwise and counterclockwise in turn. When the brightness of the field of view is consistent, record the temperature value and the dial reading in turn. Repeat this measurement 6 times. The dial reading minus the instrument zero value is the instrument measurement value. Next, according to the temperature measured each time, calculate the standard value of the optical rotation or sugar content of the standard polarimeter t℃ using the following formula: α-a201+0.000144(t-20)1
Where: α is the optical rotation or sugar content of the standard polarimeter at the measuring temperature; α20℃ is the optical rotation or sugar content of the standard polarimeter at 20℃; t is the temperature of the standard polarimeter during measurement, (3)
The average value of the difference between the instrument's measured value and the standard value (indication error) shall comply with the indication error verification specified in Table 1. According to the instrument's measuring range, the indication error verification readings are taken at +5° and -5° (+15°Z and -15\Z), +17Z and 17°Z (+50Z and -50), +35\Z and -35Z (+100°Z and -100°Z). It is required that the difference between the maximum and minimum values of the six values measured at each point shall not exceed the sensitivity tolerance. When the reading does not meet the requirements, it shall be measured and read again. (II) Verification of automatic polarimeters and automatic polarimeter sugar meters 18 Article 8 Verification shall be carried out by visual observation and manual test as required. 19 Verification of instrument indication error and repeatability According to the instrument's measuring range, the verification of instrument indication error and repeatability shall be carried out by using six standardThe maximum difference between the calibration value at 4400m wavelength and the displayed value when the standard polarimeter rotates along the tube axis,
**The calculation of expanded uncertainty should include the direction error, IV. Verification items and verification methods
(-I) Verification of otoscope polarimeters and visual polarimeter sugar meters 15. Item 24 Verification by visual observation or manual test according to the requirements of each article,
16 Verification of instrument sensitivity
After the instrument is lit according to the use requirements and the light source is normal, first adjust to find the zero point of the brightness balance of the two-thirds or three-thirds field of view, and then adjust the dial from the zero point Rotate the position left or right (3 times each) to the degree of sensitivity tolerance, and then observe whether there is a noticeable change in the brightness balance of the two-thirds or three-thirds field of view. If so, it means that the instrument recording sensitivity is qualified. For the slow sensitivity verification, just read one side of the scale values on the left and right sides of the dial. A total of 20 pages, page 8
JJG536---98
If the sensitivity reading is unqualified, it can be re-debugged or the light source lamp can be replaced and then verified again. 17 Verification of instrument indication error
Each reading in this verification, if there are two reading windows, refers to the average of the two readings on the left and right sides of the dial.
First, measure the instrument zero value when the empty test tube is closed, rotate the dial clockwise and counterclockwise in turn to achieve field brightness balance, write down the dial reading, repeat this process, and take the average of 6 readings as the instrument zero value. Put the standard polarimeter into the test tube and position it in the middle (the instrument with the test tube set at an angle needs to be fixed with plasticine), stick the probe of the digital thermometer on the body of the standard polarimeter close to the quartz plate with white tape, and connect the digital thermometer. Close the test tube. Depending on the temperature difference between the tube and the standard polarimeter, the standard polarimeter needs to be kept still in the tube for (7-10) minutes to allow the temperature of the standard polarimeter to reach equilibrium with that of the test tube. When measuring, rotate the dial clockwise and counterclockwise in turn. When the brightness of the field of view is consistent, record the temperature value and the dial reading in turn. Repeat this measurement 6 times. The dial reading minus the instrument zero value is the instrument measurement value. Next, according to the temperature measured each time, calculate the standard value of the optical rotation or sugar content of the standard polarimeter t℃ using the following formula: α-a201+0.000144(t-20)1
Where: α is the optical rotation or sugar content of the standard polarimeter at the measuring temperature; α20℃ is the optical rotation or sugar content of the standard polarimeter at 20℃; t is the temperature of the standard polarimeter during measurement, (3)
The average value of the difference between the instrument's measured value and the standard value (indication error) shall comply with the indication error verification specified in Table 1. According to the instrument's measuring range, the indication error verification readings are taken at +5° and -5° (+15°Z and -15\Z), +17Z and 17°Z (+50Z and -50), +35\Z and -35Z (+100°Z and -100°Z). It is required that the difference between the maximum and minimum values of the six values measured at each point shall not exceed the sensitivity tolerance. When the reading does not meet the requirements, it shall be measured and read again. (II) Verification of automatic polarimeters and automatic polarimeter sugar meters 18 Article 8 Verification shall be carried out by visual observation and manual test as required. 19 Verification of instrument indication error and repeatability According to the instrument's measuring range, the verification of instrument indication error and repeatability shall be carried out by using six standard
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