Some standard content:
National Standard of the People's Republic of China
Rules for agricultural seed testing-Purity analysis
1 Subject content and scope of application
GB/T3543.3-1995
Replaces GB3543---83
This standard specifies the determination method for seed purity analysis (including the determination of the number of seeds of other plants). This standard is applicable to the detection of the quality of crop seeds. 2 Reference standards
GB/T3543.2 Rules for the inspection of crop seeds-Sampling 3 Terminology
3.1 Pure seed
The seed unit or trench of the species described by the sender (including all botanical varieties and cultivars of the species) meets the requirements of Appendix A (Supplement).
3.2 Other seeds
Any plant seed unit other than clean seeds, including weed seeds and seeds of other crops. 3.3 Inert matter
Seed units other than clean seeds and other plant seeds and all other substances and structures. 4 Instruments
Clarity analysis table.
Bell-shaped sample divider, horizontal format sample divider. h.
Set sieves of different apertures (including oscillators), hair dryers. The specifications of sieves used for beet embryo seeds are shown in Appendix A (Supplement). Handheld magnifying glass or binocular microscope, etc.
Balance: The sensitivity is 0.1, 0.01, 0.001g and 0.1mg. 5 Determination Procedure
5.1 Inspection of Heavy Impurities
In the sample to be tested (or at least 10 times the weight of the sample to be analyzed for purity), if there are impurities that are obviously different in size or weight from the seeds to be tested and seriously affect the results, such as soil blocks, small stones or large seeds mixed with small seeds, etc., these heavy impurities should be picked out and weighed first. Then the heavy impurities should be separated into other plant seeds and impurities. 5.2 Separation of Test Samples
Approved by the State Administration of Technical Supervision on August 18, 1995 and implemented on June 1, 1996
GB/T 3543.3--1995
The test samples for purity analysis should be separated from the samples to be tested according to the prescribed slice method (see Article 6.2 of GB/T3543.2). The test sample should be estimated to contain at least 2500 seed units or not less than the weight specified in Table 1 of GB/T3543.2. Purity analysis can be carried out with a sample of the specified weight, or two half samples (half the weight of the sample). The test sample must be weighed in grams, accurate to the number of decimal places specified in Table 1, to meet the requirements of calculating the percentage of various components to the decimal place.
Table 1 Weighing and decimal places
Weight of sample or half sample and its components, g
Below 1.0000
1. 000~~~9.999
10.0099.99
100. 0~~999. 9
1000 or more than 1000
5.3 Separation of samples
Weigh to the following decimal places
a. After the sample is weighed, it shall be separated into three components: clean seeds, other plant seeds and impurities according to the provisions of Appendix A (Supplement). b. During separation, the sample may be examined with the aid of magnifying glasses, sieves, hair dryers and other instruments (see Chapter 4), or by applying pressure with tweezers, without damaging the germination ability.
During separation, each seed unit in the sample must be carefully examined and analyzed based on the obvious characteristics of the seeds, and identified based on morphological c
characteristics, seed specimens, etc. When it is difficult or impossible to distinguish between different plant species, the genus name shall be filled in, and all the species of the genus shall be clean seeds, with additional instructions.
d. Seed units with no obvious damage to the seed coat or pericarp, whether empty or full, shall be regarded as clean seeds or other plant seeds. If there is a crack in the seed coat or pericarp, the inspector must determine whether the remaining portion of the seed unit exceeds one-half of the original size. If such a determination cannot be made quickly, the seed unit shall be classified as clean seeds or other plant seeds. e. After separation, each component is weighed separately, expressed in grams, and converted into percentage. 6 Determination of the number of other plant seeds
Depending on the different requirements of the test sender, the number of other plant seeds can be determined by complete inspection, limited inspection and simplified inspection. 6.1 Complete inspection
The weight of the test sample shall not be less than 25,000 seed units or the weight specified in Table 1 of GB/T3543.2. With the help of magnifying glasses, sieves, hair dryers and other instruments, analyze and identify each seed according to the provisions of Appendix A (Supplement), take out all other plant seeds in the sample, and count the number of seeds of each species. When it is found that some seeds cannot be accurately determined to belong to the species, identification to the genus is allowed. 6.2 Limited inspection
The inspection method of limited inspection is the same as that of complete inspection (6.1), but it is limited to finding the species of other plant species specified by the test sender from the entire test sample. If the test sender only requires to test whether certain specified species exist, it is sufficient to find... seeds or a few seeds. 6.3 Simplified test
If the species specified by the tester is difficult to identify, a simplified test can be used. Simplified test is to use one-fifth of the weight of the specified test sample (see 6.1) (minimum amount) to identify the species. The test method of simplified test is the same as that of complete test (6.1). 7 Calculation and expression of results
7.1 Calculation of results
7.1.1 Verification of weight gain and loss during analysis
Whether it is one sample or two and a half samples, the sum of the weights of the various components after analysis should be compared with the original weight basis to verify whether there is any loss of material during the analysis. If the difference in gain and loss exceeds 5% of the original weight, it must be repeated and the results of the repeated analysis should be reported. . 39
7.1.2 Calculation of weight percentage of each component
GB/T 3543.3
When analyzing a sample, the weight percentage of all components (i.e., clean seeds, other plant seeds, and impurities) should be calculated to the right decimal. When analyzing a half sample, all components of each half sample should be calculated separately, and the percentage should be retained to at least two decimal places, and the average percentage of each component should be calculated.
The percentage must be calculated based on the sum of the weights of various components after analysis, rather than the original weight of the test sample. Other plant seeds and impurities are no longer classified for percentage calculation. 7.1.3 Check the error between replicates
7.1.3.1 Two half samples
If two half samples are analyzed, the difference in any component after analysis shall not exceed the allowable difference between replicate analyses shown in Table 2. The types of shelled seeds referred to in Table 2 are shown in Appendix B (Supplement). If the actual differences of all components are within the allowable range, calculate the average value of each component. If the actual difference exceeds the allowable range, the following procedures shall be followed: Table 2 Allowable difference in clarity analysis of the same sample submitted for inspection in the same laboratory (two-tailed determination at a 5% significance level)
Average of two analysis results
More than 50%
99. 95 ~100. 00
99.90~99.94
99.85~~99. 89
99.80~99.84
99. 75~99. 79
99. 70~99. 74
99. 65~~99. 69
99. 60~99. 64
99.55~99.59
99.50~99.54
99.40-99. 49
99.30~99.39
99.20~99.29
99. 10~~99. 19 | | tt | 99
97.50~~97.74
97. 25~~97. 49
97. 00 ~~97. 24
96. 50~96. 99
96. 00~~96. 49 | 04
0.05~~0. 09
0.10~~0.14
0.15~~0.19
0.20~0.24
0. 25~0. 29
0 . 30~0. 34
0.35~0.39
0.40~~0.44
0.45~0.49
0.50~~0.59
0. 60~0.69
0.70~0.79
0.80~~0.89
0. 90~0. 99
1.00~1.24
1. 25~~1. 49
1.50~1.74
1. 75~~1. 99
2.00~2.24
2.50~~2.74
2 . 75~~2. 99
3.00~3. 49
3.50~3.99
4.00~~4.49
4.50~4.99
5.00~~5.99
6. 00~~6. 99|| tt||7. 00~7. 99
Half sample
No 2-shell seeds
Tolerance between different measurements
With 2-shell seeds
No shell seeds||tt ||Hulled seeds
Average of two analysis results
More than 50%
91. 00~~91. 99
90. 00~~90. 99| |tt||88. 00~89. 99
86. 00~~87. 99
84.00~85.99
82. 00~83. 99
80 . 00~~81.99
78. 00~79. 99
76. 00~~77. 99
74. 00~75. 99
72. 00~73. 99
70. 00~71. 99
65. 00 ~~69. 99
60.00~~64.99
50.00~59.99
Below 50%
8.00~8. 99
9. 00~~9. 99| |tt||10. 00~11. 99
12. 00~~13. 99
14, 00~~15. 99
16. 00~17. 99
18. 00~~19. 99
20. 00~~21. 99
22. 00~~23. 99
24. 00~25 . 99
26. 00~27. 99
28.00~29.99
30. 00 ~~ 34. 99
35.00~39.99
40.00~49.99
GB/T 3543.3—1995
Continued Table 2
Allowable difference between different measurements
Half sample
Seeds without shell
Seeds with shell||tt ||Non-hulled seeds
Hulled seeds
Reanalyze the paired samples until one pair of values is within the tolerance range (but the total analysis does not need to exceed four pairs). ,||tt| |Whenever the difference between a pair exceeds twice the permissible difference, it shall be omitted. b.
The final record of the percentage of each component shall be calculated from the weighted average of all the pairs retained. c.||tt ||7.1.3.2 Two or more samples
If it is necessary to analyze a second sample under certain circumstances, the actual difference in the components of the two samples shall not exceed the difference in the components of the two samples as shown in Table 2. The types of hulled seeds referred to in Table 2 are given in Appendix B (Supplement). If all components are within the allowable range, take their average value. If they exceed, analyze another sample. If the difference between the highest value and the lowest value after analysis is not greater than twice the allowable error, report the three values. If one or more of the results are obviously due to errors, the result shall be eliminated. 7.1.4 Rounding
The final reported result of each component shall be retained to one decimal place. The sum should be 100.0%. Trace components less than 0.05% should be excluded from the calculation. If the sum is 99.9% or 100.1%, then add or subtract 0.1% from the maximum value (usually the net seed fraction). If the rounded value is greater than 0.1 %, then the calculation should be checked for errors.
7.1.5 Conversion of results with heavy impurities
Net seeds:
P,(%) = P ×
Other plant seeds:
OS(%) = OS, × 4
Impurity:
m2×100
I2(%) = I ×
Where: M-||tt| |-Weight of sample for inspection, g;
Weight of heavy mixture, g;
Weight of other plant seeds in heavy mixture, name, 1
GB/T 3543.31995
Weight of impurities in heavy impurities·g;
P-the percentage of net seed weight after removing heavy impurities, %;-the percentage of impurities weight after removing heavy impurities, %; OS ,
The weight percentage of other plant seeds after removing heavy impurities,%. The final check should be: (P2+12+0S2)%=100.0%. 7.2 Result expression
The purity analysis results are expressed in three When determining the number of seeds of other plants, the result is expressed as the number of seeds of the species (or genus) found in the determination, which can also be converted into the number of seeds per unit weight (such as per kilogram). Table 6 It can be used to determine whether there is a significant difference between two test results. However, when comparing, the weight of the two samples must be roughly the same.
8 Result Report
The results of the clarity analysis should be retained to one decimal place. The total of the percentages of the various ingredients must add up to 100%. If the weight percentage of a component is less than 0.05%, fill in "trace amount". If the result of a component is zero, fill in "0.0-". When the weight percentage of a certain type of impurity or a certain type of other plant seeds reaches or exceeds 1%, When measuring the number of seeds of other plants, the actual weight of the seeds, the scientific name and the number of seeds of each species found in the weight should be filled in on the result report. , and indicate whether full inspection, limited inspection or simplified inspection is used. Table 3 Tolerable difference in clarity analysis between samples sent for testing in the same or different laboratories
(one-tailed test at 1% significance level)
Average of two results
50 % or more
99.95~100.00
99. 90~99. 94
99. 85~99. 89
99.80~~99.84
99. 75~99.79
99.70~99.74
99. 65~~99. 69
99.60~~99.64
99.55~99.59
99.50~~99.54
99.40~99.49
99.30~99.39
99.20~ 99.29
99. 10~~99. 19
99. 00~~99. 09
98.75~98.99
98.50~~98.74
98.25~98.49
98 . 00~~98. 24
97. 75 ~~ 97. 99
Below 50%
0.00~0. 04
0.10~0. 14
0.15~0.19
0.20~~0.24||tt| |0.25~0. 29
0. 30~~0. 34
0.35~0.39
0.40~0.44
0.45~0.49
0.50~0.59
0.60~0.69
0.70~0.79| |tt||0.80~~0.89
0. 90 ~0. 99
1. 00~~ 1. 24
1.25~1.49
1. 50~1.74
1. 75 ~1. 99
2.00~~2.24
Seeds without shell
Allowable difference
Seeds with shell
More than 50%
97. 50~ 97. 74
97. 25~98. 49
97. 00~~97. 24
96. 50~96. 99
96.00~~96.49
95.50~95.99
95.00~~95. 49
94.00~~94.99
93. 00~~ 93. 99
92. 00~~92. 99
91. 00~~91. 99
90. 00~90. 99
88.00~89.99
86.00~-87.99
84.00~~85.99
82.00~83.99
80.00~~81.99
78.00~79. 99
76.00~~77.99
74.00~75.99
72. 00~73. 99
70. 00~71. 99
65.00~~69.99
60. 00~64. 99
50.00~59.99
More than 50%
99.95~100.00
99. 90~99.94
99.85~~99.89
99.80~99.84
99.75~99.79
99. 70~ 99. 74
99. 65 ~~99. 69
99.60~~99.64
99.55~99.59
99. 50~~99. 54
Average of two results
GB/T 3543.3--1995
Continued Table 3
Below 50%
2. 25~2. 49
2. 50~2. 74
2. 75~~2. 99
3.00~3. 49
3.50~3.99
4.00~~4.49
4.50~4.99
5.00~~5.99
6. 00 ~ 6. 99
7. 00~7.99
8.00~8.99bzxZ.net
9. 00 ~9. 99
10.00~~11.99
12.00~13.99
14.00~15.99
16.00~~17.99
18.00~19.99
20. 00~21. 99
22. 00~23. 99
24.00~25.99
26. 00~27. 99
28. 00~29. 99
30.00~34.99
35.00~39.99
40.0049.99
Hull-less seeds
Table 4 The allowable difference in clarity analysis between two different samples when the second test is conducted in the same or different laboratories (two-tailed test at 1% significance level)
Average of two results
Less than 50%
0. 00~0. 04
0.05~0.09
0. 10~0. 14
0.15~0.19
0.20~0.24
0.25~~0. 29
0.30~0. 34
0.35~0.39
0.40~~0.44
0.45~0.49
Seeds without shell
Allowable difference
Allowable difference
Seeds with shell
Seeds with shell
More than 50%
99.40~99.49
99. 30~~99. 39
99.20~99.29
99. 10~~99. 19
99.0099.09
98. 75 ~98. 99
98.50~98.74
98. 25~98. 49
98.00~98.24
97.75~97. 99
97.50~97.74
97. 25~-98. 49
97. 00~97. 24
96.50~~96.99
96. 00~96. 49
95.50~95.99
95.00~95.49
94. 00~~94. 99
93.00~93.99
92.00~92.99
91. 00~~91. 99
90.00~~90.99
88.00~89.99
86.00~~87.99
84.00~85.99
82. 00~83.99
80.00~~81.99
78. 00~79. 99
76. 00~~77. 99
74.00~75.99
72. 00~73. 99
70. 00~71.99
65.00~69.99
60. 00~64. 99
50.00~59.99
Average of two results
GB/T 3543.3—1995
Continued Table 4
Below 50%
0.60~0.69
0.70~0.79
0.80~~0.89
0.90~0.99
1. 00~~~1. 24
1.25~~1. 49
1. 50~~1. 74
1. 75~1. 99
2.00~2.24
2.25~~2.49
2.50~~2.74
2.75~~2.99
3.0 0~3.49
3.50~3.99
4.00~~4.49
4.50~4.99
5.00~5.99
6. 00~6. 99
7. 00~7. 99
8.00~8.99
9. 00~9. 99
10. 00~~11. 99
12.00~13.99
14.00~15.99
16.00~17.99
18. 00~19. 99
20. 00~21. 99
22.00~23.9g
24.00~25. 99
26. 00~27. 99
28. 00~29. 99
30.00~34.99
35. 00~39. 99
40.00~49.99
Seeds without shell
Tolerance
Seeds with shell
More than 50%
99.95~100.00
99. 90~ 99. 94
99.85~~99. 89
99. 80~99. 84
99. 75~99.79
99. 70~99. 74
99. 65~99. 69
99. 60~99.64
99. 55~~99. 59|| tt||99.50~99.54
99. 40--99. 49
99.30~-99.39
99.20~99.29
99.10~99. 19
99.00--99.09
98. 75~98.99
98.50~98.74
98.25~98. 49
98. 00~98. 24
97. 75~97. 99
97.50~97.74
97. 25~98. 49
97. 00~~97. 24
96.5096.99
96.00~96. 49
95.50~95.99
95.00~95.49
94. 00~94.99
93.00~~93.99
92. 00~92. 99
91.00~91.99
90. 00~90. 99
88. 0089. 99
86. 00~~87. 99
84.00~85.99
82. 00~83. 99|| tt||80. 00~81.99
78. 00~79. 99
76, 00~77. 99
74. 00~75. 99
72. 00~73. 99
70. 00 ~ 71. 99
65. 00~69. 99
60. 00~~64. 99
50.00~59.99
GB/T3543.3—1995
Table 5 The allowable difference between the clarity analysis and the standard value (one-tail determination at 5% significance level)| |tt||Standard value
50% or less
0.00~~0.04
0. 05~0. 09
0.10~0. 14||tt| |0.15~~0.19
0.20~0. 24
0. 25~~0. 29
0.30~0.34
0.35~0. 39||tt| |0.40~0. 44
0.45~0.49
0.50~0.59
0. 60~~0. 69
0. 70~~0. 79
0.80~ 0.89
0.90~0. 99
1.00~1.24
1.25~1.49
1.50~~1.74
1.75~1. 99
2. 00~2. 24|| tt||2.25~2. 49
2.50~2. 74
2. 75~2. 99
3.00~~3.49
3.50~3.99
4.00~4.49
4.50~4.99
5.00~5. 99
6 . 00~6.99
7. 00~7. 99
8.00~8. 99
9.00~9.99
10. 00~~11. 99
12. 00~13. 99
14.00 ~15.99
16. 00~17. 99
18. 00~19. 99
20. 00~21. 99
22. 00~23. 99
24. 00~25. 99
26. 00~27. 99| |tt||28. 00~~29. 99
30.00~34.99
35.00~39.99
40. 00~49. 99
Seeds without shell
Tolerance
Seeds with shell
Average of two measurement results
9~10
14 ~15
96~102
103~110
111~117
118~125
126~133
134~142
143~151
GB/T 3543.3-1995
Table 6 Tolerable difference in the determination of seed number of other plants (two-tailed determination at 5% significance level)
Tolerable difference
Average of two determination results||tt ||152~160
161~169
170~178
179~188
189~198
199~209
210~219
220~230
231~241
242~252
253~264
265~276
277~ 288||tt| |289300
301~313
314~326
327~339
340~353|| tt||354~366
367~380
381394
395~409
410~424
425~439
440454
455~469
470~485
486~5 01
502~518
519~534
Allowable gap
Average of two measurement results
15 ~17
41~45
96~104
105~113
114~122
123~131
132~141
142152
GB/T 3543.3—1995
Table 7 Tolerable difference in the determination of seed number of other plants (one-tailed determination at 5% significance level)
Tolerable difference
Average of two determination results||tt ||153~162
163~173
174186
187~198
199~210
211~223
224~ 235
236249
250~262
263~276
277~290
291 ~305
306~320
321~336
337~351
352~367
368~386
387~403
404~420
421~438||t t||439~456
457~474
475493
494~513
514~532
533~552
allowed gap
A7 term
A1.1 seed unit seed unit
GB/T 3543.3---1995
Appendix A
Net seed definition (PSD)
(Supplement)
Commonly seen Dispersal unit, including true seeds, achenes, caryopsis, schizocarps and florets. A1.2 Achene, achenium
A dry, indehiscent fruit containing one seed, with the pericarp separated from the seed coat. A1. 3 Caryopsis
Fruit whose seed coat and pericarp are tightly connected, such as naked fruit of Gramineae. A1.4 Floret
In Poaceae, refers to the inner and outer florets or mature caryopsis enclosing the male and female pistils. The floret in this standard refers to the fertile floret with or without sterile florets. A1.5 Fertile floret
A floret with functional uterus (i.e., with caryopsis). A1.6 Sterile floret
A floret lacking functional organs (i.e., lacking caryopsis). A1.7 Spikelet
A unit of Poaceae inflorescence composed of one or more florets, with the base surrounded by one or two sterile glumes. The spikelet referred to in this standard includes not only fertile florets, but also one or more fertile florets or completely sterile florets or glumes. A1.8 Schizocarp
A dry fruit that can be separated into two or more units (schizocarps) when mature. A7.9 mericarp
Part of a schizocarp, as in Apiaceae, which can be separated into two schizocarps. A1.10 pod
A cracked dry fruit, as in Leguminosae.
A1.11 cluster
A cluster of densely packed inflorescences. In Beta, part of the inflorescence. A1.12 nutlet
A small, indehiscent dry fruit containing one seed. A1.13 Accessory organs
A1.13.1 Awn, arista
A slender, erect or curved spine. In Poaceae, usually an extension of the ribs of the outer or frontal lamellae. A1.13.2 beak
A sharp extension of the fruit.
A1.13.3 Bract
A reduced leaf or scale-like structure that encloses the flower or the spikelet of the Poaceae family on the axis. A1.13.4 Glume
One of the two usually sterile bracts at the base of the Poaceae spikelet. A1.13.5 Lemma
The outer (lower) bract of a Poaceae floret. Also called the glume or outer (lower) inner of the flower. The bract enclosed on the outer side of the caryopsis. 48
A1.13.6 Palea
GB/T 3543.3—1995
The inner (upper) bract of a Poaceae floret. Also called the inner or upper shell. The bract enclosed on the inner (ventral) side of the caryopsis. A7.13.7 Invoiucre
A group of circular bracts or prickles surrounding the base of the inflorescence. A1.13.8 Perianth
The two coverings of a flower (calyx and corolla), or either of them. A1.13.9 Calyx, calyces
The outer perianth consisting of sepals.
A1.13.10 Pedicel
A short stalk of a flower on an inflorescence.
A1.13.11 Caruncle
A small projection near the micropyle.
A1.13.12 Hair
A unicellular or multicellular outgrowth of the epidermis. A1.13.13 Pappus
A type of annular fine hairs, sometimes feathery or scaly, densely borne on achenes. A1.13.14 Rachilla, rhachilla Secondary spikelet axis. In Poaceae, it refers specifically to the axis on which the florets are attached. A1.13. 15 Rachis, rhachis, rachides The main axis of the inflorescence.
A1.13.16 Stalk
The stem of any plant organ.
A2 General rules for distinguishing clean seeds, other plant seeds and foreign matter A2.1 Clean seeds
A2.1.1 The following structures that can clearly identify them as belonging to the species analyzed (except those that have become sclerotia, smut spores or nematode spores) should be regarded as clean seeds, even if they are immature, shriveled, shrunken, diseased or germinated seed units. a.
Complete seed units (see A3 for details). In the Poaceae family, seed units that are florets must have a caryopsis or naked caryopsis (lacking endosperm) that clearly contains endosperm.
Damaged seed units that are larger than half of their original size. b.
Based on the above principles, there are some exceptions in individual genera or species: A2.1.2
Leguminosae and Cruciferae, seed units with completely detached seed coats should be classified as impurities. a.
Even if there is an embryonic axis with plumules and radicles, and an attached seed coat that is larger than half of its original size, the detached cotyledons of Leguminosae seed units are classified as impurities. b.
Seed units of Beta vulgaris seeds that are larger than a certain size are classified as clean seeds. c.
d. In the genera Avena and Sorghum, attached sterile florets do not need to be removed and are classified as clean seeds. A2.2 Other plant seeds
The identification principle is the same as that of clean seeds, but the seed units of the genus Beet do not need to be screened when used as seeds of other plants, and the definition of clean seeds with genetic monoembryo can be used (see A3.14).
A2.3 Impurities
Seed units that obviously do not contain true seeds.
The size of the seed units of the genus Beet with multiple embryos does not reach the minimum size specified in the definition of clean seeds. b.
The fragments of the broken or damaged seed units are half or less than half of the original size. 49rachides The main axis of the inflorescence.
A1.13.16 Stalk
The stem of any plant organ.
A2 General rules for distinguishing clean seeds, other plant seeds and impurities A2.1 Clean seeds
A2.1.1 The following structures can clearly identify them as belonging to the species analyzed (except those that have become sclerotia, smut spore masses or nematode spores), even if they are immature, shrunken, diseased or germinated. a.
Complete seed units (see A3 for details). In the Poaceae family, seed units such as florets must have a caryopsis with obvious endosperm or naked caryopsis (lacking endosperm).
Damaged seed units that are more than half of their original size. b.
According to the above principles, there are some exceptions in individual genera or species: A2.1.2
Leguminosae and Cruciferae, seed units with completely detached seed coats should be classified as impurities. a.
Even if there is an embryonic axis with plumule and radicle, and the attached seed coat is more than half of the original size, the detached cotyledons of the leguminous seed units are also classified as impurities. b.
Seed units of Beta vulgaris with multiple embryos exceeding a certain size are classified as clean seeds. c.
d. In Avena and Sorghum, the attached sterile florets do not need to be removed and are classified as clean seeds. A2.2 Other plant seeds
The identification principle is the same as that of clean seeds, but the seed units of Beta vulgaris do not need to be screened when used as seeds of other plants, and can be defined as clean seeds of genetic monoembryonic embryos (see A3.14).
A2.3 Impurities
Seed units that obviously do not contain true seeds.
The size of the unit of the re-embryo of the beet genus does not reach the minimum size specified in the definition of clean seeds. b.
The fragments of the broken or damaged seed unit are half or less than half of the original size. 49rachides The main axis of the inflorescence.
A1.13.16 Stalk
The stem of any plant organ.
A2 General rules for distinguishing clean seeds, other plant seeds and impurities A2.1 Clean seeds
A2.1.1 The following structures can clearly identify them as belonging to the species analyzed (except those that have become sclerotia, smut spore masses or nematode spores), even if they are immature, shrunken, diseased or germinated. a.
Complete seed units (see A3 for details). In the Poaceae family, seed units such as florets must have a caryopsis with obvious endosperm or naked caryopsis (lacking endosperm).
Damaged seed units that are more than half of their original size. b.
According to the above principles, there are some exceptions in individual genera or species: A2.1.2
Leguminosae and Cruciferae, seed units with completely detached seed coats should be classified as impurities. a.
Even if there is an embryonic axis with plumule and radicle, and the attached seed coat is more than half of the original size, the detached cotyledons of the leguminous seed units are also classified as impurities. b.
Seed units of Beta vulgaris with multiple embryos exceeding a certain size are classified as clean seeds. c.
d. In Avena and Sorghum, the attached sterile florets do not need to be removed and are classified as clean seeds. A2.2 Other plant seeds
The identification principle is the same as that of clean seeds, but the seed units of Beta vulgaris do not need to be screened when used as seeds of other plants, and can be defined as clean seeds of genetic monoembryonic embryos (see A3.14).
A2.3 Impurities
Seed units that obviously do not contain true seeds.
The size of the unit of the re-embryo of the beet genus does not reach the minimum size specified in the definition of clean seeds. b.
The fragments of the broken or damaged seed unit are half or less than half of the original size. 49
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