Guidelines for the conduct of tests for distinctness,uniformity and stability Plum
Some standard content:
ICS 65. 020. 20
National Standard of the People's Republic of China
GB/T19557.8\u20142004
Guidelines for the conduct of tests for distinctness, uniformity and stability\u2014Plum (Prunus spp.)
Issued on June 11, 2004
General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China Administration of Standardization of the People's Republic of China
Implemented on December 1, 2004
GB/T 19557.8\u20142004
Appendices A, B and C of this standard are normative appendices. This standard is issued by the Ministry of Agriculture of the People's Republic of China. Drafting units of this standard: Science and Technology Development Center of the Ministry of Agriculture, Institute of Fruit Research, Chinese Academy of Agricultural Sciences. The main contributors to this standard are: Zhang Jingru, Wang Rufeng, Lu Zhicheng, Chen Ruming, Gong Wenhong, Li Zhiqiang, Yu Xianghe, Li Ying, Liu Ning. 1 Scope
GB/T 19557.B--2004
Guidelines for testing specificity, consistency and stability of new plant varieties This standard specifies the technical requirements for testing specificity, consistency and stability of new plant varieties, the criteria for determining test results, and the content and format of technical reports.
This standard applies to the testing and evaluation of specificity, consistency and stability of Prurus 5sPp. varieties. 2 Normative references
The clauses in the following documents become clauses of this standard through reference in this standard. For any dated referenced document, all subsequent amendments (excluding errata) or revisions are not applicable to this standard. However, parties to an agreement based on this standard are encouraged to study whether to use the latest versions of these documents. For any undated referenced document, the latest version shall apply to this standard. GB/T19557.1-2000 Guidelines for testing specificity, consistency and stability of new plant varieties General Principles 3 Terms and definitions
Terms and definitions established in GB/T19557.1~-2004 apply to this standard. 4 Requirements for materials for test varieties
4.1 Quality and quantity of test materials
Test materials are divided into scions and seedlings. The types of materials required for the applicant to submit for testing shall be determined by the approval authority based on the test requirements. If the new variety applied for testing has special uses, the quality and quantity of the test materials submitted can be determined by the approval authority based on its characteristics and scope of application.
4. 1. Scion
Scion should be healthy, free of pests and diseases, and free of quarantine objects, and obtained by non-in vitro propagation methods. The number shall be at least 50, and each root shall have at least 10 full and plump buds (excluding hidden buds). 4.1.2 Yellowwood
Seedlings shall be free of diseases and insects, quarantine objects, and healthy, with a seedling height of 0.9m~1m and a base diameter of more than 1cm. The number shall be no less than 20.
4.2 Instructions for the treatment of test variety materials
The submitted test materials shall not be treated in any way that may affect their growth without the approval of the approval authority. 4.3 Preservation of test varieties
After receiving the test materials, the testing unit shall condense them immediately for review and examination. 4.4 Others
In addition to submitting the test materials, the applicant shall also fill in the technical volume according to the appendix. 5 Test
5.1 Test time
The test duration shall be at least two normal growth cycles, and these two growth cycles shall be able to show the normal characteristics of this variety. 5.2 Test location
The conditions at the test point should be able to meet the normal growth of the plant and the normal expression of its traits. Generally, each tested variety is arranged to be tested at the same test point. If there are special requirements, multi-point testing can be carried out. For special purposes, additional tests can be set up. Additional tests should be carried out in the identification unit or specific ecological zone designated by the approval authority. GB/T19557.8\u20142004
5.3 Requirements for sticky wood
The high-grafted mother trees used as rootstocks should be of the same variety, strong and healthy, free of pests and diseases, and have basically the same tree vigor, age and growth conditions. 5.4 Field design
The field design of the applied variety and similar varieties should be scientific, correct, reasonable, consistent, and have the same test conditions. The test can adopt the comparison method, inter-comparison method and high-grafting comparison method, with a plant-row spacing of 3m\u00d74m or 3m\u00d75m. Each plot should have no less than 5 plants, at least three replicates, and a protective row. In special cases, single-plant plots can also be used, with multiple replicates. 5.5 Field management
Field management measures for the test variety and similar varieties should be strictly consistent, and detailed implementation rules should be formulated. If the test is conducted in a greenhouse environment, detailed instructions should be provided.
6 Observation and discrimination of traits
6.1 Basic requirements for observation and discrimination
The approval authority shall determine the test traits of the variety based on the new variety information provided by the applicant. Variety observation shall be carried out in accordance with the requirements of Appendix A and Appendix B. The standard variety observed together with the test variety shall perform normally, otherwise, this test is invalid.
Observation records shall be carried out in accordance with the requirements of GB/T19557.1-2004. The discrimination of specificity, consistency and stability shall be carried out in accordance with the requirements of GB/T19557.1-2004. 6.2 Discrimination of specificity
If the same trait of the test variety and similar varieties is in the same code, it means that the test variety has no difference with the similar varieties in this trait, otherwise, there is a difference. If the test variety has two or more individual or quantitative traits that are different from similar varieties, or one of the quantitative traits differs from similar varieties by two or more codes, the test variety can be determined to be specific to the similar varieties. 6. 3 Discrimination of consistency
For the observation of variety consistency, the code is used as the analysis unit. If there are differences in the observed traits, the value of the trait with the most expression shall prevail and be reported. Traits that cannot be tested individually will not be subjected to consistency determination. 6. 4 Discrimination of stability
For the observation of variety stability, in the test results of the same growing season, if the expression of the same trait of the test variety is within the same code, it means that the variety is stable in this trait; otherwise, it is unstable. 7 Traits
7.1 Overview
The test traits include plant morphological characteristics, agronomic traits, quality traits and pest resistance traits, totaling 67 (see Appendix A for description of traits), divided into mandatory traits and supplementary traits. Mandatory traits are basic traits that must be observed and described for each test variety, while supplementary traits are traits that are further tested when mandatory traits cannot distinguish the test variety from similar varieties. 7.2 Mandatory traits (28)
Tree bud, leaf shape, leaf tip shape, base shape, petiole length, leaf size, leaf color, flower buds on annual branches, corolla size, petal shape, stigma position, fruit size, fruit shape, position of the maximum transverse diameter of the fruit, peel base color, peel color, fruit suture line, fruit symmetry, fruit top shape, flesh color, soluble solid content, fresh food quality, flesh and stone adhesion, stone relative to fruit size, stone shape, flowering period, fruit ripening period, leaf fall period. 7.3 Supplementary traits (39)
Tree vigor, tree shape, new shoot length, new shoot internode length, new shoot color, bud growth posture, leaf bud size, leaf bud shape, bud receptacle size, etc. Young leaf color, leaf margin serration, petiole groove depth, petiole hairs, nectary glands, number of magic glands, location of nectary glands, shape of nectary glands, leaf length, leaf length-width ratio, leaf surface gloss, leaf back hairs, petal overlap, petal upper edge shape, petal posture, petal color, pedicel depth, pedicel width, petal length, peel thickness, fruit hardness, pulp juice, pulp fiber, kernel size, kernel symmetry (waist view, lateral diameter \u00d7 length), kernel symmetry (length \u00d7 width), kernel widest position, kernel surface, kernel base width, kernel back status. Trait categories and definitions
1. Tree posture\(J,U)
2. Tree vigor (J,U)
4. New length
5. Internode length of new shoots (U)
6. Color of new shoots (J)
7. Size of leaf buds.)
8. Shape of leaf buds (J, U)
Observation period
Appendix A
(Normative Appendix)
Tested traits of plum varieties
Trait table
Trait description
Half a piece
Cup-shaped
Natural open heart shape
Round head shape
Spindle shape
Dispersed shape
Cluster shape
Conical shape
Oval
Long and male shape
GB/T 19557.8\u20142004
Standard product weighing
Black gem, red flesh plum
Dwarf school red plum, Suiting No. 3
Dwarf sweet plum
Lianjin plum, Chifeng purple plum
Lianjin plum
Angeno, Longyuan autumn plum
Changli No. 15, Black gem
Sui Li No. 3, yellow skin plum
Suilou red plum, big yellow plum
Italy six, pearl plum
Italy five, black spicy amber
Dwarf sweet season, Lianjin plum
Yaohuang plum, Huixian red plum
Small yellow plum, North No. 2
Changli No. 15 No., Jijin Li
Sui Li No. 3, Longyuan Autumn Li
Longyuan Autumn Li, Chaoyang Shilixiang
Renshi Caosheng
Sui Li No. 3, Meigui Shihou
Beifang No. 2
Yuejin Li
Jilin Limei, Mingshui Yellow Li
Suilou Red Li, Red Heart Li
Hollywood, Red Leaf Li
Dwarf Sweet Li
Sui Li No. 3
Niu Xin Li (Yellow
Plum Plum, Lingbao Li
Zaomei, Sui Li No. 3
Kuiban, Yuanmi Li
GB/T 19557.8---2004
Character categories and definitions
9. Leaf bud growth posture (J, U)
10. Bud holder size (U)
11. Young leaf color (U)
12. Leaf shape (J, U)
13. Leaf tip shape (, U)
14. Leaf base shape (U)
15. Leaf margin serration (J, U)
16. Petiole length (F, U)
17. Depth of petiole groove
18. Hairs under petiole
Testing period
Table A.1 (continued)
Character description
Semi-attached
Needle-shaped
Inverted branch-enclosed shape
Run inverted egg garden
Long Tuyi
Narrow inlaid shape
Standard and elegant varieties
Hongpao plum, Sui Li No. 3
Chang Li No. 15
Rose Queen, Black Li
Rose Queen
Jilin Late Red
Sui Li No. 3 No., Kekoutian
Kekoutian, Zaozhuokai
Dashi Caosheng, Qiyan Li
Changli No. 13, Caomeili
Yuejin Li
Dwarf Sweet Li
Muhong Sweet Li, Sui Li No. 3
Dameigui
Dashi Zaosheng
Changli No. 15
Meili Li
Jinxi Qiuli
Dwarf Sweet Li
Zaomeili, Honggou Li
Muhong Nai Li, Dwarf Sweet Li
Zaoyan, Xunyan Li
Caomeili
Shennong Li, Kekoutian
Jilin Wanhong
Sui Li No., Hongpao Li
Jinxi Qiuli, Sui Li No. 3 No.
Jilin Guihong
Dashi Zaosheng
Beautiful Plum
Changli No. 15
Jilin Wanhong
Character category and definition
19. Egg gland
Nectary gland shape
Nectary gland number (U)
22. Location of pelvic gland
23. Leaf size*
24. Leaf length
25. Leaf length-width ratio (U)
26. Leaf color*U)
27. Leaf surface gloss (J, U)
28. Leaf back hairs (3, U)
29. Flower buds on annual branches
30. Palm leaf change (U
31. Berry leaf color selection
Observation period
Table A1 (continued)
Character description
>2 pcs
leaf base
leaf base and petiole
curved simple pedicel
GB/T 19557.8\u20142004
standard promotion varieties
Sui Li No. 3, Chang Li No. 15 No.
Jinxi Autumn Plum
Jilin Late Red, Longyuan Autumn Plum
Delicious and sweet, Longjunmi Plum
Jilin Late Red, Hongpeng Plum
Delicious and sweet
Jilin Late Red, Jinxi Autumn Plum
Longyang Yingshi, Red Flower Plum
Small Yellow Plum, Ye
Lianjin Plum
Sui Li No. 3, American Niu Xin Plum
Small Yellow Plum
Zao Meili
Sui Li No. 3
Hong Pao Plum
Sui Li No. 3 No., Dashi Zaosheng
Yuejin Plum
Beautiful Plum, Jinxi Qiuli
Changli No. 15. Dashi Zaosheng
Kekoutian, Hanzhong Tongxia Plum
Hollywood, Red Leaf Plum
Jilin Red
Black Gem
Suileng Hunjiao Plum
Sui Li No. 3
Sui Li 3 No., Longyuan Autumn Plum
Apricot Plum, Little Red Plum
Banana Plum
Zao Meili
Dashi Dry Plum
Sui Li 3
Hong Pao Plum
Xiang Yan Plum
Sui School Fragrance Season
.........
CB/T19557.8\u20142004
Character category and definition
32. Corolla size (J, U)
33. Petal shape (J, U)
34. Flower splash height condition
35. Petal upper edge shape
36. Stigma position (J,U)
(relative to anther)
37. Fruit size {F,U)
38. Fruit shape (J,U)
39. Position of the maximum transverse diameter of the fruit*
40. Fruit shape\{J,U)
41. Stem injection detection (J, U)
Observation period
Capsule A.1 (continued)
Character summary
Obovate
Wide sugar round
Serrate
Court shape
Oval
Oval
Heart shape
Odd fruit stalk
Near the middlebzxz.net
Odd fruit neck
Standard variety
Northeast beauty
Dashi Musheng, Hongpao Li
Zhishi No. 3
Dashi Caosheng, Ermei
Niuxin Li
Jilin Wanhong, Sui Li 3 No.
Banana Plum
Dayou Kensheng
Jilin Late Red
Changdong No. 15, Banana Plum
Changli No. 15
Zaomeier
Huanli No. 3 No., Xiangbianji
Northeast Meiji, Suixiao Xiangyanli
Xiangyanghong, Xiaoduli
Suijiehongli, Dayu Zaosheng
Furongli
Heilongbo
Heibaoshi
Longyuanqiuli
Sui Li No. 3
Litaode Grass-grown
Dashi Dry-grown
Red Heart Li
Dwarf Xie Li, Red Heart Li
Sui Li No. 3, Longyuanqiu
Late Black, Richard Zaoniu
Heileishi, Changli 15 No.
Sui Li No. 3, Suixiao Red Li
Dayou Dry Li
Red Heart Li
Hulan Red Li
Dawan Li
Yingli Li, July Fragrance
Character categories and definitions
42. Width of the sag
43. Length of the fruit stalk
44. Peel base color (J, U)
45. Peel color (I, U)
46. Peel thickness (U)
47. Fruit chain line (U)
48. Fruit symmetry (J, U)
49. Flesh gingival color* (J, U)
Observation period
Table A. 1 (continued)
Character description
Asymmetry
GB/T 19557.8\u20142004
Standard varieties
Dashi Gensheng
Sui Li No. 3, Chang Li No. 15
Longyuan Qiuli, July Fragrance
Black Gemstone.Fuli
Amikan
Cherry Plum
Suileng Red Plum July Fragrance
Apricot Plum,Small yellow dry core
Hulan red plum, Yunnan Qingcuilai
hollow plum, Qing
Kenbaihua
early moonlight, apricot plum
Sui Li 3 each, Dashi early
red heart
black gem, Australian plum 14
Dashi early, North No. 2
Saoyan Hongpao
Sui Li No. 3
Beijing late red, Huoshizhongsheng
Sui Li No. 3, Hongxinxue
Dashi early moonlight plum
Xiangyan plum. Late red
early moonlight, wheat yellow plum
Sui Li No. 3, small yellow plum
Beijing late red, fragrant plum
red heart plum, Youdi plum
GB/T 19557.B\u20142004
Characteristics and definitions
50. Fruit firmness (J)
51. Pulp juice (U)
52. Pulp fiber content
53. Soluble matter content\
54. Fresh food quality*
55. Pulp-core adhesion
56. Core size relative to fruit*
57. Size of fruit
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