Some standard content:
GB/T8967—2000
This standard adopts the "monosodium glutamate" standard in the sixth edition of the Japanese "Food Additives Code" in 1994.
This standard is a revision of GB/T8967—1988 "monosodium glutamate". The main differences between this standard and GB/T8967-1988 are as follows:
The standard name is changed to "monosodium glutamate (99% monosodium glutamate)";-The limit on zinc is cancelled;
-The "heavy metal (in terms of Pb)" indicator is changed to "lead", and the maximum limit shall not exceed 1mg/kg; The sulfate indicator is changed from less than (or equal to) 0.03% to less than (or equal to) 0.05%; The rapid method for determining the loss on drying (second method) is added; Other relevant test methods are also adjusted accordingly:
The quality requirements of semi-finished L-glutamic acid (acid) are included in Appendix A. Appendix A and Appendix B of this standard are both indicative appendices. This standard shall replace GB/T8967-1988 from the date of implementation. This standard is proposed by the State Bureau of Light Industry.
This standard is under the jurisdiction of the National Food Fermentation Standardization Center. The drafting units of this standard are: China Food Fermentation Industry Research Institute, Shenyang Hongmei Enterprise Group Co., Ltd., Shanghai Guanshengyuan Tianchu Food Co., Ltd., Guangzhou Aosang MSG Food Co., Ltd. The main drafters of this standard are: Tian Qijing, Zhang Shigen, Yu Rujun, Su Zhenyu, Hua Huiying, Chen Jigao, Xu Aijun.
This standard is interpreted by the National Food Fermentation Standardization Center. Approved by the State Administration of Quality and Technical Supervision on April 5, 2000 and implemented on September 1, 2000
1 Scope
GB/T8967--2000
National Standard of the People's Republic of China
Monosodium L-glutamate (99% MSG)
Monosodium L-glutamate (99% WeiJing) GB/T8967-2000
Replaces GB/T8967-1988
This standard specifies the definition, technical requirements, test methods, inspection rules and marking, packaging, transportation and storage requirements of monosodium glutamate (99% MSG). This standard applies to products with a monosodium glutamate content of not less than 99%. 2 Referenced Standards
The provisions contained in the following standards constitute the provisions of this standard through reference in this standard. When this standard was published, the versions shown were all valid. All standards are subject to revision. Parties using this standard should explore the possibility of using the latest versions of the following standards. GB191—1990 Pictorial markings for packaging, storage and transportation
GB/T601—1988 Preparation of standard solutions for titration analysis (volume analysis) of chemical reagents GB/T602—1988 Preparation of standard solutions for determination of impurities in chemical reagents GB/T603—1988 Preparation of preparations and products used in test methods for chemical reagents GB1354—1986 Rice
GB/T5009.11—1996 Determination of total in food GB/T5009.12—1996 Determination of lead in food GB/T6543—1986 Corrugated paper boxes
GB/T6682—1992 Specifications and test methods for water used in analytical laboratories GB7718—1994 General standard for food labeling GB9687—1988 Polymers for food packaging Hygienic standard for vinyl molded products GB/T12309—1990 Industrial corn starch
QB/T1840—1993 Industrial potato starch
State Administration of Technical Supervision Order [1995] No. 43 Regulations on the Supervision of Calculation of Quantitative Packaged Goods 3 Definitions
This standard adopts the following definitions
Sodium glutamate (99% MSG) sodium L-glutamate; monosodium L-glutamate monohydrate; abbreviation: MSG (99% WeiJing) is a white crystal or powder with special umami flavor made from carbohydrates (starch, rice, molasses and other sugars) as raw materials, fermented, extracted, neutralized and crystallized by microorganisms (Corynebacterium glutamicum, etc.). 4 Chemical name, molecular formula, structural formula, molecular weight Chemical name: L-glutamate monosodium monohydrate (or L-α-aminoglutaric acid monosodium monohydrate) Molecular formula: CsHsNNa04·H20
Approved by the State Administration of Quality and Technical Supervision on April 5, 2000 and implemented on September 1, 2000
GB/T 8967-2000
Structural formula: NaOOC—CHCH2CH—COOH·H,ONH2
Relative molecular mass: 187.13 (according to the relative atomic mass in 1995) 5 Technical requirements
5.1 Raw material requirements
Corn starch: It should meet the requirements of GB/T12309. Potato starch: It should meet the requirements of QB/T1840. Rice: It should meet the requirements of GB1354.
Semi-finished product L-glutamic acid (glutamic acid): shall meet the requirements of Appendix A. 5.2 Appearance and sensory requirements
This product is colorless to white crystals or powder, without obvious impurities, with a special fresh taste and no odor. 5.3 Physical and chemical requirements
It shall meet the requirements of Table 1.
Sodium glutamate content, %
Transmittance, %
Specific rotation, [al
Chloride (as C1), %
Loss on drying, %
Iron, mg/kg
Sulfate (as SO?-), %
Arsenic, mg/kg
Lead, mg/kg
6 Test method
Physical and chemical indexes of sodium glutamate
+24.9~+25.3°
The laboratory water used in this test method shall comply with the specifications of grade 3 or above in GB/T6682. All reagents used are analytically pure unless otherwise specified. 6.1 Appearance and sensory inspection
Sprinkle the sample on white filter paper, visually inspect its color and impurities, and taste its flavor. For the same unit package, no more than 1 (0.5 mm or larger) impurity visible to the naked eye shall be found in 100g of sample. 6.2 Identification of sodium glutamate
If necessary, identification may be carried out according to Appendix B.
6.3 Content of sodium glutamate
6.3.1 Method 1 Titration method with non-aqueous solution of perchloric acid 6.3.1.1 Summary of method
In the presence of acetic acid, titrate the sodium glutamate in the sample with perchloric acid and determine the endpoint by potentiometric titration, or use α-naphthol phenylmethanol as an indicator and titrate the solution until it turns green as the endpoint. 6.3.1.2 Reagents and solutions
Approved by the State Administration of Quality and Technical Supervision on April 5, 2000, and implemented on September 1, 2000
GB/T8967-2000
a) Perchloric acid standard solution [c(HCIO4)=0.1mo1/L]: prepared and calibrated according to 4.23 of GB/T601-1988;
b) Acetic acid (GB/T676);
c) Formic acid (HG/T3-1296);
d2g/Lα-phenylmethanol-acetic acid indicator solution: weigh 0.1g of α-naphtholmethanol, dissolve it in acetic acid [b)] and dilute to 50mL.
6.3.1.3 Instruments
Automatic potentiometric titrator (accuracy ± 5Mv); or acidometer: use glass electrode as indicator electrode, saturated calomel electrode as reference electrode (or composite electrode), and prepare electromagnetic stirrer. 6.3.1.4 Analysis steps
a) First method potentiometric titration
First, treat the electrodes and calibrate the potentiometric titrator according to the instrument manual. Weigh 0.15g of sample in a small beaker, accurate to 0.0001g, add 13mL of formic acid [c), stir until completely dissolved, then add 30mL of acetic acid [b), shake well. Place the small beaker containing the test solution on an electromagnetic stirrer, insert the electrode, start stirring, and add 0.5 mL of perchloric acid standard solution [a)] from the burette in batches, while recording the potential E (or pH value) and the volume V of the perchloric acid standard solution consumed; when the titration is about to reach the end point, add 0.05 mL of perchloric acid standard solution each time, record the potential E (or pH value) and the volume V of the perchloric acid standard solution consumed once, and continue to add perchloric acid standard solution after the sudden jump point, until there is no obvious change in the potential E (or pH value).
Use the potential E (or pH value) as the ordinate and the volume V of the perchloric acid standard solution consumed during the titration as the abscissa to draw the EV titration curve, and take the turning point (sudden jump point) of the curve as the titration end point. b) Second method indicator method
Weigh 0.15 g of sample, accurate to 0.0001g, add 3mL of formic acid [c)], stir until completely dissolved, then add 30mL of acetic acid [b)] and 10 drops of α-naphthol phenylmethanol-acetic acid indicator solution [d)], and titrate the test solution with perchloric acid standard solution [a) 1 until the color turns green, which is the end point, and record the volume of perchloric acid standard solution consumed (V). At the same time, do a blank test and record the volume of perchloric acid standard solution consumed (V.). c) Calibration of perchloric acid solution concentration
If the temperature difference between the titration sample and the calibration of the perchloric acid solution exceeds 10°C, the concentration of the perchloric acid solution must be recalibrated; if it does not exceed 10°C, it should be corrected according to formula (1). Co
cj=1+ 0.0011x(t-1)
wherein: c
-concentration of perchloric acid solution when titrating the test solution, mol/L; co
concentration of perchloric acid solution during calibration, mol/L; temperature of perchloric acid solution when titrating the test solution, ℃; temperature of perchloric acid solution during calibration, ℃; 0.0011——expansion coefficient of acetic acid.
6.3.1.5 Expression of analysis results
The content of sodium glutamate in the sample is calculated according to formula (2): 0.09357×(VV.)××100
Wherein: X is the content of sodium glutamate in the sample, %; Approved by the State Administration of Quality and Technical Supervision on April 5, 2000·(1)
Implementation on September 1, 2000
GB/T8967—2000
is the volume of perchloric acid standard solution consumed by the test solution, mL; Vi
Vo is the volume of perchloric acid standard solution consumed by the blank, mL; c is the concentration of perchloric acid standard solution, mol/L; 0.09357 is the mass of sodium glutamate (CsH:NNaO4·H20) equivalent to 1.00mL perchloric acid standard solution [c(HCIO4)=1.000mol/L], g; sample mass, g.
The calculation result shall be accurate to the first decimal place. 6.3.1.6 Allowable difference
The relative average deviation of the measurement result of the same sample shall not exceed 0.3%. 6.3.2 Second method Polarimetry
6.3.2.1 Method summary
The molecular structure of sodium glutamate contains an asymmetric carbon atom, which is optically active and can rotate the polarized light plane by a certain angle. Therefore, its optical rotation can be measured by a polarimeter. The content of sodium glutamate is converted according to the optical rotation.
6.3.2.2 Reagents
Hydrochloric acid (GB/T622)
6.3.2.3 Instruments
Polarimeter (accuracy ± 0.01°) is equipped with a sodium lamp (sodium spectrum D line 589.3nm). 6.3.2.4 Analysis steps
a) Weigh 10g of sample, accurate to 0.0001g, add a small amount of water to dissolve and transfer all into a 100mL volumetric flask, add 20mL of hydrochloric acid, mix well, wait until it cools to 20℃, add water to the scale, and shake well. b) In a constant temperature room (20℃), first calibrate the instrument with the standard optical rotation angle. Then, place the above test solution in a polarimeter tube (no bubbles), observe its optical rotation, and record the temperature of the test solution in the polarimeter tube. 6.3.2.5 Expression of analysis results
The content of sodium glutamate in the sample is calculated according to formula (3): a
X,=25.16+0.047(201)
Wherein: Xz is the content of sodium glutamate in the sample,%; a
is the actual optical rotation of the test solution;
L is the length of the polarimeter tube (i.e. the thickness of the liquid layer), dm;-1mL The mass of sodium glutamate in the test solution, g/mL; 25.16 is the specific optical rotation of sodium glutamate, [a]; - the temperature of the test solution during the determination, °C;
0.047 is the temperature correction coefficient.
The calculation result is accurate to the first decimal place. 6.3.2.6 Allowable difference
The relative average deviation of the determination results of the same sample shall not exceed 0.3%. 6.4 Transmittance
6.4.1 Instrument
Spectrophotometer (accuracy ± 0.5T%).
6.4.2 Analytical procedures
Approved by the State Administration of Quality and Technical Supervision on April 5, 2000 (3)
Implemented on September 1, 2000
GB/T 8967-2000
Weigh 10 g of sample to the nearest 0.1 g, dissolve in water and make up to 100 mL, shake well, and use this as the test solution. Rinse with the test solution and inject into 10 mm colorimetric blood. Use the same batch of water used to dissolve the sample to adjust the instrument zero point and measure its transmittance at a wavelength of 430 nm.
Measurement results are accurate to integers.
6.4.3 Permissible difference
The absolute value difference between two measurements of the same sample shall not exceed 1%. 6.5 Specific rotation
6.5.1 Summary of method
Same as 6.3.2.1.
6.5.2 Reagents
Same as 6.3.2.2.
6.5.3 Apparatus
Same as 6.3.2.3.
6.5.4 Analytical procedures
Same as 6.3.2.4.
6.5.5 Expression of analysis results
If the sodium spectrum D line is used, 1dm polarimeter tube, when measuring at 20℃ (liquid temperature is 20℃), the reading can be directly read; if the test solution temperature is t℃, it must be converted according to formula (4): X, = [a] - 0.047 (20-t)
Where: X3 specific rotation of the sample, al20; [al—specific rotation of the test solution at t℃; t—temperature of the test solution during measurement, ℃
0.047—temperature correction coefficient.
The calculation result is accurate to the first decimal place. 6.5.6 Allowable difference
The absolute value difference between two determinations of the same sample shall not exceed 0.02°6.6 Chloride
6.6.1 Method summary
The trace chloride ions contained in the test solution react with silver nitrate to form silver chloride precipitation, and its turbidity is compared with the silver chloride produced by standard chloride ions, and visual turbidimetric quantification is performed. 6.6.2 Reagents and solutions
a) Nitric acid (GB/T626);
b) Chloride standard solution (1 mL solution contains 0.1 mg chloride): Prepare according to 4.30 of GB/T602-1988.
c) 10% (V/V) nitric acid solution: Measure 1 volume of nitric acid [a)] and inject it into 9 volumes of water; d) Silver nitrate standard solution [c(AgNO3)=0.1 mol/L]: Prepare according to 4.21 of GB/T601-1988.
6.6.3 Analysis steps
Weigh 10g of sample, accurate to 0.1g, dissolve in water and make up to 100mL, shake well, as test solution.
Pipette 10.00mL of test solution into a 50mL Nessler colorimetric tube, add 13mL of water, shake well; Accurate State Administration of Quality and Technical Supervision Approved on April 5, 2000, implemented on September 1, 2000
GB/T8967-2000
Pipette 110.00mL of chloride standard solution [b] into another 50mL Nessler colorimetric tube, add 13mL of water, shake with a spoon; at the same time, add 1mL of 10% (V/V) nitric acid solution [c) and 1.00mL of silver nitrate standard solution [d] to each of the above two tubes, shake well immediately, place in a dark place for 5min, take out, and immediately perform horizontal or vertical visual turbidity comparison.
If the turbidity of the sample tube is not higher than that of the standard tube, the chloride content is equal to or lower than 0.1%. 6.7 pH value
6.7.1 Method summary
Immerse the indicator electrode and the reference electrode in the same solution to be tested to form a primary cell. At a certain temperature, the electromotive force of the primary cell is in a linear relationship with the pH value of the solution. The pH value of the solution can be obtained by measuring the electromotive force of the primary cell.
6.7.2 Reagents
Phosphate standard buffer solution (pH value is 6.86): Weigh 3.40g of potassium dihydrogen phosphate (KH, PO4) and 3.55g of disodium hydrogen phosphate (Na2HPO4) that have been dried at 120℃ for 2h, dissolve in water without carbon dioxide and make up to 1000mL, and shake well.
6.7.3 Instruments
a) pH meter (acidity meter): accuracy ± 0.02 pHb) Electrode: glass electrode as indicator electrode, saturated calomel electrode as reference electrode (or composite electrode).
6.7.4 Analysis steps
Use phosphate standard buffer solution, at 25°C, calibrate the pH meter to a pH value of 6.86, locate (or use two-point location method), and rinse the electrode with water. Weigh 5g of sample, accurate to 0.1g, dissolve in water without carbon dioxide and make up to 50mL, shake well, and use it as the test solution. Wash the electrode with the test solution, then insert the electrode into the test solution, adjust the pH meter temperature compensation knob to 25℃, and measure the pH value of the test solution. Repeat the operation until the pH value reading is stable for 1min, and record the result.
The measurement result is accurate to the first decimal place. 6.7.5 Allowable difference
The absolute value difference between two measurements of the same sample shall not exceed 0.05pH. 6.8 Loss on drying
6.8.1 Summary of method
The mass of volatile substances (adherent moisture, etc.) lost by the drying method is determined, expressed as a percentage.
6.8.2 Method 1 Conventional method
6.8.2.1 Apparatus
a) Electric drying oven: temperature controlled at 98℃±1℃; b) Weighing bottle: 50mm×30mm;
c) Desiccator: filled with silica gel desiccant
d) Analytical balance: sensitivity 0.1mg.
6.8.2.2 Analytical steps
Weigh 5g of sample with a weighing bottle that has been dried to constant weight, accurate to 0.0001g, place in an electric drying oven at 98℃±1℃, dry for 5h, take out, cover, place in a desiccator, cool to room temperature (30min), and weigh.
6.8.2.3 Expression of analysis results
The drying loss of the sample shall be calculated according to formula (5): Approved by the State Administration of Quality and Technical Supervision on April 5, 2000 and implemented on September 1, 2000
GB/T8967-2000
X4=ml-m2x100
Wherein: X4—the drying loss of the sample, %; the mass of the weighing bottle, g;
the mass of the weighing bottle and the sample before drying, g; mr
the mass of the weighing bottle and the sample after drying, g; the calculation result shall be accurate to the first decimal place. 6.8.2.4 Allowable difference
The relative average deviation of the measurement results of the same sample shall not exceed 10%. 6.8.3 Method 2 Rapid Method
6.8.3.1 Apparatus
a) Electric drying oven: temperature control (103±2)℃; b) Weighing bottle, desiccator, analytical balance: same as 6.8.2.1. 6.8.3.2 Analysis steps
Use a weighing bottle that has been dried to constant weight to weigh 5g of sample, accurate to 0.0001g, place in an electric drying oven at 103℃±2℃, dry for 2h, take out, cover, place in a desiccator, cool to room temperature (30min) and weigh.
6.8.3.3 Expression of analysis results
Same as 6.8.2.3.
6.8.3.4 Tolerance
Same as 6.8.2.4.
6.9.1 Method Summary
Under acidic conditions, the iron ions in the sample solution react with ammonium thiocyanate to generate blood-red ferric thiocyanate, the depth of which is proportional to the concentration of iron ions, and can be used for colorimetric determination. 6.9.2 Reagents and solutions
a) Nitric acid (GB/T626)
b) 1+1 nitric acid solution: measure 1 volume of nitric acid [a)] and inject it into 1 volume of water; c) Ammonium thiocyanate (GB/T660);
d) 150g/L ammonium thiocyanate solution: weigh 15.0g of ammonium thiocyanate [c)], dissolve it in water and make up to 100mL;
e) Iron standard solution (1mL solution contains 0.1mg iron): prepare the stock solution according to 4.55 in GB/T602-1988. When using, dilute accurately 10 times to obtain the standard iron solution (1 mL of solution contains 0.01 mg of iron).
6.9.3 Analysis steps
Weigh 1 g of sample accurately to 0.1 g, place in a 50 mL Nessler colorimetric tube, add 10 mL of water, shake to dissolve, then add 2 mL of nitric acid solution [b)] and shake well; accurately pipette 0.05 mL of the standard iron solution [e)] into another 50 mL Nessler colorimetric tube, add 9.5 mL of water and 2 mL of nitric acid solution [b)] and shake well. Boil the two tubes in a boiling water bath for 20 minutes, take them out, cool them to room temperature with running water, add 10.00 mL of ammonium thiocyanate solution [d) to each tube, add water to the 25 mL mark, shake well, and use white paper as the background for visual color comparison.
If the color of the sample tube is not higher than that of the standard tube, the iron content is equal to or lower than 5 mg/kg. 6.10 Sulfate
Approved by the State Administration of Quality and Technical Supervision on April 5, 2000, and implemented on September 1, 2000
6.10.1 Summary of the method
GB/T8967—2000
The trace amount of sulfate in the sample solution reacts with barium chloride to form a white barium sulfate precipitate, which is quantitatively compared with the standard turbidity.
6.10.2 Reagents and solutions
a) Hydrochloric acid (GB/T622)
b) 10% (V/V) barium hydrochloride solution: measure 1 volume of hydrochloric acid [a)] and inject it into 9 volumes of water; c) Barium chloride (GB/T652);
d) 50g/L barium chloride solution: weigh 5.0g of barium chloride [c)], add water, stir and dissolve, and make up to 100mL;
e) Sulfate standard solution (1mL solution contains 0.1mg sulfate): prepare according to 4.28 of GB/T6.21988.
6.10.3 Analysis steps
Weigh 0.5g of sample, accurate to 0.01g, place in a 50mL Nessler colorimetric tube, add 18ml of water to dissolve, then add 2mL of hydrochloric acid solution [b) and shake to mix; accurately pipette 2.50mL of sulfate standard solution [e)], place in another 50mL Nessler colorimetric tube, add 15.5mL of water and 2mL of hydrochloric acid solution [b)], shake to mix. At the same time, add 5.00mL of barium chloride solution [d)] to each of the above two tubes, shake well, place in a dark place for 10min, take out and perform visual turbidity comparison.
If the turbidity of the sample tube is not higher than that of the standard tube, the sulfate content is equal to or lower than 0.05%. 6.11 Arsenic
Weigh 1g of sample, accurate to 0.01g, place in a conical flask for arsenic measurement, add 25mL of water and shake to dissolve, as the test solution. The following is determined according to the arsenic spot method (second method) in GB/T5009.11-1996. 6.12 Lead
Weigh 1g of sample, accurate to 0.01g, dissolve in water and make up to 50mL, shake well, and use it as test solution without digestion. The following is determined according to the atomic absorption spectrometry (first or second method) or disulfide colorimetry (third method) in GB/T5009.12-1996. 6.13 Unit packaging quality inspection
Lift the unit (inner) packaging bag, seal it downward, and shake it gently 3 times. The contents must not leak. 7 Inspection rules
7.1 Batch
All products with the same manufacturer name, product name, specification, trademark and batch number, and the same quality certificate are considered a batch.
7.2 Sampling
Sampling samples and unit packaging according to Table 2. The total amount of samples should not be less than 500g. If it is less than 500g, it can be added in proportion.
Batch range
2~25
151~1200
1201~35000
35001 and above
Number of samples to be drawn
Number of unit packages to be drawn
Bag, bottle or barrel
Number of qualified judgments"Number of unqualified judgments
After sampling, first conduct a piece-by-piece inspection of the appearance and unit packaging quality. 7.2.1
Approved by the State Administration of Quality and Technical Supervision on April 5, 2000 R
Implementation on September 1, 2000
GB/T8967-2000
7.2.2Open the unit packaging bag, mix quickly, and pack into two clean, dry ground-mouth bottles. Label them and indicate the sample name, manufacturer name, trademark, production date (batch number), sampling date, location and name of the sampler. One bottle is sent to the laboratory for inspection, and the other bottle is sealed for 3 months for future reference. 7.3 Delivery inspection
7.3.1Before the product leaves the factory, the technical inspection department of the manufacturer shall inspect each batch according to the provisions of this standard. Products that meet the requirements of this standard and issue quality certificates can leave the factory. 7.3.2 Delivery inspection items
Appearance and sensory, sodium glutamate content, light transmittance, specific rotation, pH value, loss on drying, iron, sulfate, unit packaging mass, label and net content. 7.4 Routine inspection
7.4.1 The inspection items include all the items required by the technical requirements of this standard. 7.4.2 Under normal circumstances, routine inspections are conducted once a year. In any of the following circumstances, routine inspections must also be conducted.
a) Changes in major raw and auxiliary materials;
b) Changes in key processes and equipment;
c) New trial products or when normal production is stopped and resumed; d) National technical supervision agencies put forward routine inspection requirements. 7.5 Judgment rules
7.5.1 Delivery inspections and routine inspections are judged in the same way. 7.5.2 Appearance and unit packaging quality are judged according to Table 2. 7.5.3 Labels, net content and other physical and chemical indicators, when one of the inspection results does not meet the requirements of this standard, samples of twice the amount should be taken from the same batch of products for re-inspection, and the re-inspection results shall prevail. If one or more items are still unqualified, the entire batch of products shall be judged as unqualified. 8 Marking, packaging, transportation, storage
8.1 Marking
8.1.1 The packaging, storage and transportation graphic markings shall comply with the provisions of GB191. 8.1.2 The outer packaging box shall indicate: product name, manufacturer name, factory address, production (packaging) date (or batch number), specifications, total net content, and shall be marked with words such as "waterproof, moisture-proof" and "handle with care". 8.1.3 The sales packaging shall be marked in accordance with the provisions of GB7718: product name, manufacturer name, factory address, sodium glutamate content, production (packaging) date, net content and product standard number implemented. The shelf life (or storage period) can be exempted from marking; the production (packaging) date can be marked with year, month and day by spraying or stamping, but the handwriting must be clearly visible. 8.2 Packaging
8.2.1 Products for sale must be packaged in plastic film bags that meet the requirements of GB9687 or containers that meet the requirements of food hygiene. Unpackaged products shall not be put on the market for sale. The packaging cartons must comply with the requirements of GB/T6543. 8.2.2 The net content of the sales packaging shall comply with the provisions of the State Technical Supervision Bureau Order [1995] No. 43. 8.2.3 The packaging bags should be tightly sealed.
8.3 Transportation and storage
8.3.1 The products should be handled with care during transportation and should not be thrown, smashed or bumped. 8.3.2 During transportation, the products should be protected from rain, moisture and sunlight, and should not be mixed or transported with toxic, harmful, odorous, corrosive items and other pollutants. 8.3.3 The product shall not be stored together with toxic, harmful, odorous, corrosive articles and other pollutants during storage.
Approved by the State Administration of Quality and Technical Supervision on April 5, 2000 and implemented on September 1, 2000
A1 Definition
GB/T 8967-2000
Appendix A
(Indicative Appendix)
Quality requirements for semi-finished L-glutamic acid (glutamic acid) This standard adopts the following definitions.
L-glutamic acid (glutamic acid) L-glutamic acid is a crystal or crystalline powder with a characteristic sour taste, made from surface hydrates (starch, rice, molasses and other sugars) fermented and purified by microorganisms (Corynebacterium glutamicum, etc.). A2 Technical requirements
A2.1 Requirements for main raw materials
Corn starch: It shall comply with the provisions of GB/T12309. Potato starch: should comply with the provisions of QB/T1840. Rice: should comply with the provisions of GB1354.
Molasses: Enterprise standards must be formulated to control its sugar content and impurities. A2.2 Appearance and sensory requirements
This product is off-white, light yellow (referring to starch, rice, etc. as raw materials) or brown yellow (referring to molasses as raw materials) crystals or crystalline powders, with a slightly characteristic sour taste and no peculiar smell. A2.3 Physical and chemical requirements
Should comply with the provisions of Table 1.
L-glutamic acid content, %
Specific rotation, [al
Transmittance, %
Sulfate (SO-), %
1) Molasses as raw material.
A3 Test method
A3.1 L-glutamic acid content
A3.1.1 Method 1: Polarimetry
A3.1.1.1 Summary of method
Same as 6.3.2.1.
A3.1.1.2 Reagents
Same as 6.3.2.2.
A3.1.1.3 Apparatus
Same as 6.3.2.3.
A3.1.1.4 Analysis steps
a) Preparation of test solution
L-glutamic acid (glutamic acid) physical and chemical indicators
Superior quality
Qualified quality
Weigh 10g of sample, accurate to 0.0001g, add 20mL of water, add 16.5mL of hydrochloric acid (GB/T622) under stirring, dissolve it completely and transfer it into a 100mL volumetric flask. When the solution cools to 20℃, add water to make up to volume and mix well. Filter with filter paper and collect the filtrate. Approved by the State Administration of Quality and Technical Supervision on April 5, 2000, implemented on September 1, 2000
GB/T 8967-2000
If the test solution is dark in color, add 0.1g of activated carbon (when molasses is used as the raw material and the color is very dark, a maximum of 0.3g of activated carbon can be added) and stir to decolorize. Filter with filter paper, discard the first 5 mL of filtrate, and collect the rest of the filtrate as the test solution.
b) Determination
Determine according to 6.3.2.4b).
A3.1.1.5 Expression of analysis results
The L-glutamic acid content in the sample is calculated according to formula (A1): a
XA=32.00+ 0.06(201)
Wherein: Xu
Glutamic acid content in the sample, %;
Actual optical rotation of the test solution;
Length of the polarimeter tube (i.e., thickness of the liquid layer), dm;-1mL mass of glutamic acid in the test solution, g/mL;32.00
Specific optical rotation of glutamic acid, [a] 20;
t——Temperature of the test solution during determination, ℃;
0.06——Temperature correction coefficient.
Calculation result shall be accurate to the first decimal place. A3.1.1.6 Allowable difference
Same as 6.3.2.6.
A3.1.2 Second method Neutralization titration method
A3.1.2.1 Method summary
Glutamic acid has two acidic one-COOH groups and one basic one-NH, group. One of the COOH groups can be titrated with alkaline solution, and the glutamate content can be indirectly obtained by the amount of consumed alkali. A3.1.2.2 Reagents and solutions
a) Sodium hydroxide standard solution [c(Na0H)=0.1mol/L]: Prepare and calibrate according to 4.1 of GB/T601-1988:
b) Sodium hydroxide standard solution [c(Na0H)=0.05mol/L]: Dilute the sodium hydroxide standard solution [a)] accurately by 1 time.
A3.1.2.3 Instrument
Automatic potentiometric titrator.
A3.1.2.4 Analysis steps
a) Process the electrodes and calibrate the automatic potentiometric titrator according to the instrument manual. b) Roughly weigh 10g of the sample and grind it into a fine powder in a mortar as the test sample. c) Accurately weigh 0.25g of the sample to an accuracy of 0.0001g, place it in a 100mL tall beaker, add 70mL of water and heat to dissolve it completely, cool to room temperature, and use potentiometric titration to titrate with 0.05mol/L sodium hydroxide standard solution [b]]. The endpoint pH value is controlled at 7.0, and the volume (V) of the sodium hydroxide standard solution consumed is recorded. d) Simultaneously perform a blank test and record the volume (V) of the sodium hydroxide standard solution [b] consumed. A3.1.2.5 Expression of analysis results
The content of L-glutamic acid in the sample is calculated according to formula (A2): 0.1471xcx(VV)
Approved by the State Administration of Quality and Technical Supervision on April 5, 2000·(A2)
Implementation on September 1, 20003 The sales package should be marked in accordance with GB7718: product name, manufacturer name, factory address, sodium glutamate content, production (packaging) date, net content and product standard number. The shelf life (or storage period) can be exempted; the production (packaging) date can be marked with year, month and day by spraying or stamping, but the handwriting must be clearly visible. 8.2 Packaging
8.2.1 The sales product must be packaged in plastic film bags that meet the requirements of GB9687 or containers that meet the food hygiene requirements. Unpackaged products shall not be put on the market. The packaging cartons must meet the requirements of GB/T6543. 8.2.2 The net content of the sales package shall comply with the provisions of the State Administration of Technical Supervision Order [1995] No. 43. 8.2.3 The packaging bag should be tightly sealed.
8.3 Transportation and storage
8.3.1 The product should be handled with care during transportation and must not be thrown, smashed or knocked. 8.3.2 During transportation, the product shall be protected from rain, moisture and sunlight, and shall not be mixed or transported with toxic, harmful, odorous, corrosive substances and other pollutants. 8.3.3 During storage, the product shall not be mixed with toxic, harmful, odorous, corrosive substances and other pollutants
Approved by the State Administration of Quality and Technical Supervision on April 5, 2000, implemented on September 1, 2000
A1 Definition
GB/T 8967-2000
Appendix A
(Indicative Appendix)
Quality Requirements for Semi-finished L-Glutamic Acid (Glutamic Acid) This standard adopts the following definitions.
L-glutamic acid (glutamic acid) L-glutamic acid is made from surface hydrates (starch, rice, molasses and other sugars) as raw materials, fermented and purified by microorganisms (Corynebacterium glutamicum, etc.), and is a crystal or crystalline powder with a characteristic sour taste. A2 Technical requirements
A2.1 Requirements for main raw materials
Corn starch: It should comply with the provisions of GB/T12309. Potato starch: It should comply with the provisions of QB/T1840. Rice: It should comply with the provisions of GB1354.
Molasses: Enterprise standards must be formulated to control its sugar content and impurities. A2.2 Appearance and sensory requirements
This product is off-white, light yellow (referring to starch, rice, etc. as raw materials) or brown yellow (referring to molasses as raw materials) crystals or crystalline powders, with a slight characteristic sour taste and no odor. A2.3 Physical and chemical requirements
It should comply with the provisions of Table 1.
L-glutamic acid content, %
Specific rotation, [al
Transmittance, %
Sulfate (SO-), %
1) Molasses as raw material.
A3 Test method
A3.1 L-glutamic acid content
A3.1.1 Method 1 Polarimetry
A3.1.1.1 Summary of method
Same as 6.3.2.1.
A3.1.1.2 Reagents
Same as 6.3.2.2.
A3.1.1.3 Apparatus
Same as 6.3.2.3.
A3.1.1.4 Analysis steps
a) Preparation of test solution
L-glutamic acid (glutamic acid) physical and chemical indicators
Superior quality
Qualified quality
Weigh 10g of sample, accurate to 0.0001g, add 20mL of water, add 16.5mL of hydrochloric acid (GB/T622) under stirring, dissolve it completely and transfer it into a 100mL volumetric flask. When the solution cools to 20℃, add water to make up to volume and mix well. Filter with filter paper and collect the filtrate. Approved by the State Administration of Quality and Technical Supervision on April 5, 2000, implemented on September 1, 2000
GB/T 8967-2000
If the test solution is dark in color, add 0.1g of activated carbon (when molasses is used as the raw material and the color is very dark, a maximum of 0.3g of activated carbon can be added) and stir to decolorize. Filter with filter paper, discard the first 5 mL of filtrate, and collect the rest of the filtrate as the test solution.
b) Determination
Determine according to 6.3.2.4b).
A3.1.1.5 Expression of analysis results
The L-glutamic acid content in the sample is calculated according to formula (A1): a
XA=32.00+ 0.06(201)
Wherein: Xu
Glutamic acid content in the sample, %;
Actual optical rotation of the test solution;
Length of the polarimeter tube (i.e., thickness of the liquid layer), dm;-1mL mass of glutamic acid in the test solution, g/mL;32.00
Specific optical rotation of glutamic acid, [a] 20;
t——Temperature of the test solution during determination, ℃;
0.06——Temperature correction coefficient.
Calculation result shall be accurate to the first decimal place. A3.1.1.6 Allowable difference
Same as 6.3.2.6.
A3.1.2 Second method Neutralization titration method
A3.1.2.1 Method summary
Glutamic acid has two acidic one-COOH groups and one basic one-NH, group. One of the COOH groups can be titrated with alkaline solution, and the glutamate content can be indirectly obtained by the amount of consumed alkali. A3.1.2.2 Reagents and solutions
a) Sodium hydroxide standard solution [c(Na0H)=0.1mol/L]: Prepare and calibrate according to 4.1 of GB/T601-1988:
b) Sodium hydroxide standard solution [c(Na0H)=0.05mol/L]: Dilute the sodium hydroxide standard solution [a)] accurately by 1 time.
A3.1.2.3 Instrument
Automatic potentiometric titrator.
A3.1.2.4 Analysis steps
a) Process the electrodes and calibrate the automatic potentiometric titrator according to the instrument manual. b) Roughly weigh 10g of the sample and grind it into a fine powder in a mortar as the test sample. c) Accurately weigh 0.25g of the sample to an accuracy of 0.0001g, place it in a 100mL tall beaker, add 70mL of water and heat to dissolve it completely, cool to room temperature, and use potentiometric titration to titrate with 0.05mol/L sodium hydroxide standard solution [b]]. The endpoint pH value is controlled at 7.0, and the volume (V) of the sodium hydroxide standard solution consumed is recorded. d) Simultaneously perform a blank test and record the volume (V) of the sodium hydroxide standard solution [b] consumed. A3.1.2.5 Expression of analysis results
The content of L-glutamic acid in the sample is calculated according to formula (A2): 0.1471xcx(VV)
Approved by the State Administration of Quality and Technical Supervision on April 5, 2000·(A2)
Implementation on September 1, 20003 The sales package should be marked in accordance with GB7718: product name, manufacturer name, factory address, sodium glutamate content, production (packaging) date, net content and product standard number. The shelf life (or storage period) can be exempted; the production (packaging) date can be marked with year, month and day by spraying or stamping, but the handwriting must be clearly visible. 8.2 Packaging
8.2.1 The sales product must be packaged in plastic film bags that meet the requirements of GB9687 or containers that meet the food hygiene requirements. Unpackaged products shall not be put on the market. The packaging cartons must meet the requirements of GB/T6543. 8.2.2 The net content of the sales package shall comply with the provisions of the State Administration of Technical Supervision Order [1995] No. 43. 8.2.3 The packaging bag should be tightly sealed.
8.3 Transportation and storage
8.3.1 The product should be handled with care during transportation and must not be thrown, smashed or knocked. 8.3.2 During transportation, the product shall be protected from rain, moisture and sunlight, and shall not be mixed or transported with toxic, harmful, odorous, corrosive substances and other pollutants. 8.3.3 During storage, the product shall not be mixed with toxic, harmful, odorous, corrosive substances and other pollutants
Approved by the State Administration of Quality and Technical Supervision on April 5, 2000, implemented on September 1, 2000
A1 Definition
GB/T 8967-2000
Appendix A
(Indicative Appendix)
Quality Requirements for Semi-finished L-Glutamic Acid (Glutamic Acid) This standard adopts the following definitions.
L-glutamic acid (glutamic acid) L-glutamic acid is made from surface hydrates (starch, rice, molasses and other sugars) as raw materials, fermented and purified by microorganisms (Corynebacterium glutamicum, etc.), and is a crystal or crystalline powder with a characteristic sour taste. A2 Technical requirements
A2.1 Requirements for main raw materials
Corn starch: It should comply with the provisions of GB/T12309. Potato starch: It should comply with the provisions of QB/T1840. Rice: It should comply with the provisions of GB1354.
Molasses: Enterprise standards must be formulated to control its sugar content and impurities. A2.2 Appearance and sensory requirements
This product is off-white, light yellow (referring to starch, rice, etc. as raw materials) or brown yellow (referring to molasses as raw materials) crystals or crystalline powders, with a slight characteristic sour taste and no odor. A2.3 Physical and chemical requirements
It should comply with the provisions of Table 1.
L-glutamic acid content, %
Specific rotation, [al
Transmittance, %
Sulfate (SO-), %
1) Molasses as raw material.
A3 Test method
A3.1 L-glutamic acid content
A3.1.1 Method 1 Polarimetry
A3.1.1.1 Summary of method
Same as 6.3.2.1.
A3.1.1.2 Reagents
Same as 6.3.2.2.
A3.1.1.3 Apparatus
Same as 6.3.2.3.
A3.1.1.4 Analysis steps
a) Preparation of test solution
L-glutamic acid (glutamic acid) physical and chemical indicators
Superior quality
Qualified quality
Weigh 10g of sample, accurate to 0.0001g, add 20mL of water, add 16.5mL of hydrochloric acid (GB/T622) under stirring, dissolve it completely and transfer it into a 100mL volumetric flask. When the solution cools to 20℃, add water to make up to volume and mix well. Filter with filter paper and collect the filtrate. Approved by the State Administration of Quality and Technical Supervision on April 5, 2000, implemented on September 1, 2000
GB/T 8967-2000
If the test solution is dark in color, add 0.1g of activated carbon (when molasses is used as the raw material and the color is very dark, a maximum of 0.3g of activated carbon can be added) and stir to decolorize. Filter with filter paper, discard the first 5 mL of filtrate, and collect the rest of the filtrate as the test solution.
b) Determination
Determine according to 6.3.2.4b).
A3.1.1.5 Expression of analysis results
The L-glutamic acid content in the sample is calculated according to formula (A1): a
XA=32.00+ 0.06(201)
Wherein: Xu
Glutamic acid content in the sample, %;
Actual optical rotation of the test solution;
Length of the polarimeter tube (i.e., thickness of the liquid layer), dm;-1mL mass of glutamic acid in the test solution, g/mL;32.00
Specific optical rotation of glutamic acid, [a] 20;
t——Temperature of the test solution during determination, ℃;
0.06——Temperature correction coefficient.
Calculation result shall be accurate to the first decimal place. A3.1.1.6 Allowable difference
Same as 6.3.2.6.
A3.1.2 Second method Neutralization titration method
A3.1.2.1 Method summary
Glutamic acid has two acidic one-COOH groups and one basic one-NH, group. One of the COOH groups can be titrated with alkaline solution, and the glutamate content can be indirectly obtained by the amount of consumed alkali. A3.1.2.2 Reagents and solutions
a) Sodium hydroxide standard solution [c(Na0H)=0.1mol/L]: Prepare and calibrate according to 4.1 of GB/T601-1988:
b) Sodium hydroxide standard solution [c(Na0H)=0.05mol/L]: Dilute the sodium hydroxide standard solution [a)] accurately by 1 time.
A3.1.2.3 Instrument
Automatic potentiometric titrator.
A3.1.2.4 Analysis steps
a) Process the electrodes and calibrate the automatic potentiometric titrator according to the instrument manual. b) Roughly weigh 10g of the sample and grind it into a fine powder in a mortar as the test sample. c) Accurately weigh 0.25g of the sample to an accuracy of 0.0001g, place it in a 100mL tall beaker, add 70mL of water and heat to dissolve it completely, cool to room temperature, and use potentiometric titration to titrate with 0.05mol/L sodium hydroxide standard solution [b]]. The endpoint pH value is controlled at 7.0, and the volume (V) of the sodium hydroxide standard solution consumed is recorded. d) Simultaneously perform a blank test and record the volume (V) of the sodium hydroxide standard solution [b] consumed. A3.1.2.5 Expression of analysis results
The content of L-glutamic acid in the sample is calculated according to formula (A2): 0.1471xcx(VV)
Approved by the State Administration of Quality and Technical Supervision on April 5, 2000·(A2)
Implementation on September 1, 20003 Physical and chemical requirements
Should meet the requirements of Table 1. bZxz.net
L-glutamic acid content, %
Specific optical rotation, [al
Transmittance, %
Sulfate (SO-), %
1) Molasses as raw material.
A3 Test method
A3.1 L-glutamic acid content
A3.1.1 First method Polarimetry
A3.1.1.1 Summary of method
Same as 6.3.2.1.
A3.1.1.2 Reagents
Same as 6.3.2.2.
A3.1.1.3 Instrument
Same as 6.3.2.3.
A3.1.1.4 Analysis steps
a) Preparation of test solution
L-glutamic acid (glutamic acid) physical and chemical indicators
Superior quality
Qualified quality
Weigh 10g of sample, accurate to 0.0001g, add 20mL of water, add 16.5mL of hydrochloric acid (GB/T622) under stirring, dissolve it completely and transfer it into a 100mL volumetric flask. When the solution cools to 20℃, add water to make up to volume and mix well. Filter with filter paper and collect the filtrate. Approved by the State Administration of Quality and Technical Supervision on April 5, 2000, implemented on September 1, 2000
GB/T 8967-2000
If the test solution is dark in color, add 0.1g of activated carbon (when molasses is used as the raw material and the color is very dark, a maximum of 0.3g of activated carbon can be added) and stir to decolorize. Filter with filter paper, discard the first 5 mL of filtrate, and collect the rest of the filtrate as the test solution.
b) Determination
Determine according to 6.3.2.4b).
A3.1.1.5 Expression of analysis results
The L-glutamic acid content in the sample is calculated according to formula (A1): a
XA=32.00+ 0.06(201)
Wherein: Xu
Glutamic acid content in the sample, %;
Actual optical rotation of the test solution;
Length of the polarimeter tube (i.e., thickness of the liquid layer), dm;-1mL mass of glutamic acid in the test solution, g/mL;32.00
Specific optical rotation of glutamic acid, [a] 20;
t——Temperature of the test solution during determination, ℃;
0.06——Temperature correction coefficient.
Calculation result shall be accurate to the first decimal place. A3.1.1.6 Allowable difference
Same as 6.3.2.6.
A3.1.2 Second method Neutralization titration method
A3.1.2.1 Method summary
Glutamic acid has two acidic one-COOH groups and one basic one-NH, group. One of the COOH groups can be titrated with alkaline solution, and the glutamate content can be indirectly obtained by the amount of consumed alkali. A3.1.2.2 Reagents and solutions
a) Sodium hydroxide standard solution [c(Na0H)=0.1mol/L]: Prepare and calibrate according to 4.1 of GB/T601-1988:
b) Sodium hydroxide standard solution [c(Na0H)=0.05mol/L]: Dilute the sodium hydroxide standard solution [a)] accurately by 1 time.
A3.1.2.3 Instrument
Automatic potentiometric titrator.
A3.1.2.4 Analysis steps
a) Process the electrodes and calibrate the automatic potentiometric titrator according to the instrument manual. b) Roughly weigh 10g of the sample and grind it into a fine powder in a mortar as the test sample. c) Accurately weigh 0.25g of the sample to an accuracy of 0.0001g, place it in a 100mL tall beaker, add 70mL of water and heat to dissolve it completely, cool to room temperature, and use potentiometric titration to titrate with 0.05mol/L sodium hydroxide standard solution [b]]. The endpoint pH value is controlled at 7.0, and the volume (V) of the sodium hydroxide standard solution consumed is recorded. d) Simultaneously perform a blank test and record the volume (V) of the sodium hydroxide standard solution [b] consumed. A3.1.2.5 Expression of analysis results
The content of L-glutamic acid in the sample is calculated according to formula (A2): 0.1471xcx(VV)
Approved by the State Administration of Quality and Technical Supervision on April 5, 2000·(A2)
Implementation on September 1, 20003 Physical and chemical requirements
Should meet the requirements of Table 1.
L-glutamic acid content, %
Specific optical rotation, [al
Transmittance, %
Sulfate (SO-), %
1) Molasses as raw material.
A3 Test method
A3.1 L-glutamic acid content
A3.1.1 First method Polarimetry
A3.1.1.1 Summary of method
Same as 6.3.2.1.
A3.1.1.2 Reagents
Same as 6.3.2.2.
A3.1.1.3 Instrument
Same as 6.3.2.3.
A3.1.1.4 Analysis steps
a) Preparation of test solution
L-glutamic acid (glutamic acid) physical and chemical indicators
Superior quality
Qualified quality
Weigh 10g of sample, accurate to 0.0001g, add 20mL of water, add 16.5mL of hydrochloric acid (GB/T622) under stirring, dissolve it completely and transfer it into a 100mL volumetric flask. When the solution cools to 20℃, add water to make up to volume and mix well. Filter with filter paper and collect the filtrate. Approved by the State Administration of Quality and Technical Supervision on April 5, 2000, implemented on September 1, 2000
GB/T 8967-2000
If the test solution is dark in color, add 0.1g of activated carbon (when molasses is used as the raw material and the color is very dark, a maximum of 0.3g of activated carbon can be added) and stir to decolorize. Filter with filter paper, discard the first 5 mL of filtrate, and collect the rest of the filtrate as the test solution.
b) Determination
Determine according to 6.3.2.4b).
A3.1.1.5 Expression of analysis results
The L-glutamic acid content in the sample is calculated according to formula (A1): a
XA=32.00+ 0.06(201)
Wherein: Xu
Glutamic acid content in the sample, %;
Actual optical rotation of the test solution;
Length of the polarimeter tube (i.e., thickness of the liquid layer), dm;-1mL mass of glutamic acid in the test solution, g/mL;32.00
Specific optical rotation of glutamic acid, [a] 20;
t——Temperature of the test solution during determination, ℃;
0.06——Temperature correction coefficient.
Calculation result shall be accurate to the first decimal place. A3.1.1.6 Allowable difference
Same as 6.3.2.6.
A3.1.2 Second method Neutralization titration method
A3.1.2.1 Method summary
Glutamic acid has two acidic one-COOH groups and one basic one-NH, group. One of the COOH groups can be titrated with alkaline solution, and the glutamate content can be indirectly obtained by the amount of consumed alkali. A3.1.2.2 Reagents and solutions
a) Sodium hydroxide standard solution [c(Na0H)=0.1mol/L]: Prepare and calibrate according to 4.1 of GB/T601-1988:
b) Sodium hydroxide standard solution [c(Na0H)=0.05mol/L]: Dilute the sodium hydroxide standard solution [a)] accurately by 1 time.
A3.1.2.3 Instrument
Automatic potentiometric titrator.
A3.1.2.4 Analysis steps
a) Process the electrodes and calibrate the automatic potentiometric titrator according to the instrument manual. b) Roughly weigh 10g of the sample and grind it into a fine powder in a mortar as the test sample. c) Accurately weigh 0.25g of the sample to an accuracy of 0.0001g, place it in a 100mL tall beaker, add 70mL of water and heat to dissolve it completely, cool to room temperature, and use potentiometric titration to titrate with 0.05mol/L sodium hydroxide standard solution [b]]. The endpoint pH value is controlled at 7.0, and the volume (V) of the sodium hydroxide standard solution consumed is recorded. d) Simultaneously perform a blank test and record the volume (V) of the sodium hydroxide standard solution [b] consumed. A3.1.2.5 Expression of analysis results
The content of L-glutamic acid in the sample is calculated according to formula (A2): 0.1471xcx(VV)
Approved by the State Administration of Quality and Technical Supervision on April 5, 2000·(A2)
Implementation on September 1, 2000
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