QB/T 2738-2005 Evaluation method for antibacterial and antimicrobial effects of daily chemical products
Some standard content:
[CS 71.100.35
Sub-model number: Y43
Registration number: 16419-2005
Light Industry Standard of the People's Republic of China
QB/T2738-2005
Methods for evaluating daily chemical productsin antibacterial and bacteriostatic effects efficac32005-07-26 Issued
National Development and Reform Commission of the People's Republic of China Implemented on January 1, 2006
Normative references
Terms and definitions.
4 Experimental length and basic points for testing the antibacterial and antimicrobial effects of chemical products 5 Special product collection...
Product antibacterial effect evaluation original post 7 Product antibacterial and antimicrobial effect test methods 7.1 Chemical and antimicrobial effect test method for type 2 chemical products (liquid quantitative method) 7.3 Antibacterial effect test method for type 2 chemical products (liquid quantitative method) 4. Test methods for the antibacterial or antimicrobial efficacy of chemical detergents (simulation method) 7.5. Test method for the antibacterial effect of endogenous chemical disinfectants (simulation method) 7.6. Test method for the antibacterial effect of chemical products (disinfection method) 1. Test methods for the antibacterial and antimicrobial efficacy of chemical products: Appendix A (Data Record) 2.1. Determination of the content of effective chlorine in the product A.2. Determination of the content of active gas A.3. Determination of the content of effective base A.4. Determination of the content of cationic surfactant A.5. Determination of the content of resistant protein A.6 (4 A. Determination of the content of pyridine sulfide-N-(3,4-dihydro-1H-indole
A. Determination of the content of 8-chlorophenyl ether
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QB/T2738-2005
In the standard, the China Light Industry Quality Association proposed this standard. At present, this standard has a national standard for detergents with surfactants. QB/T.2738—2005
This standard was drafted by: National Detergent Production Supervision and Testing Center (Taiwan), Guangkai Blue Moon Charging Co., Ltd., Xi'an Factory Co., Ltd., State-owned Enterprise Central Commission for Discipline Inspection Co., Ltd., Jiangnan Development Group Co., Ltd., Dongfang Microbiological Analysis and Testing Center. Drafters of this standard: Zhang Shenglian, Shu Shangqi, Zhou Gaohuan, Jin Yihua, Mi Huisu, Ou Dianniu. The standard was issued at the time of publication. 1 Scope. Evaluation method for antibacterial and antimicrobial effects of daily chemical products QB/T 2738--2005. This standard specifies the detection method and evaluation standard for the antibacterial and antimicrobial effects of daily chemical products with special functions. This standard is applicable to the antibacterial and antimicrobial performance tests of cleaning products and human skin cleansing products. Other high-end chemical products may also choose to adopt it. 2 Normative references. The following clauses in the document are converted into clauses of the standard by reference in this standard. This is the current document of the standard, and all revised documents (including errata) or revised versions do not apply to the standard. Of course, the market encourages all parties that have reached an agreement on the use of the latest versions of these documents. For any documents without an unspecified date, the latest version of this standard shall apply. QB/Non-2?39-2 (filter products with the same test method titration analysis (penetration analysis) test case preparation 3 Terms and definitions
The following terms and definitions apply to the standard.
Antibacterial
The process of killing bacteria that hinder the growth and activity of the bacteria by chemical or physical methods. 3. 2
Bacterial Inhibition
Inhibits the growth of bacteria by chemical or biological methods and the generation of their activity. 3.3
Carrier
Test Microorganisms Support:
Bioburden
The total number of live microorganisms carried on a unit of the test object. Killing rate
In the limit biocidal test, the number of organisms is the value of the reduction. Note: Killing rate is expressed as % and is not expressed as %. |Killing time dLingtime
When used for identification of biological indicator resistance, it refers to the shortest time for the test indicator to grow sterile after the killing of all bacteria.
Note: Killing can be indicated by wind
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QB-T2738-2008
Seedling formation unit colonyformlngunltsingle you big life protection, culture design, single or black integrated body culture growth center to the commercial, I for the formation of the market, to really live this textbook: 3. 9
Niu Aizer
In the micro-three fast killing source test, it is used to test the concentration of microorganisms and killing agents on the surface of the product, and the effect of killing agents on the surface of the product is to produce the point of infection. 3. 9
and the product produetorneutralization control and ritual and the product of the sound agent.
4 Ten test daily chemical products anti-transmission, inhibition of the actual gold propaganda and aseptic operation of the requirements of 41 cattle real night plan closed cloth, unique sound solution ten clean wine, disinfection: in order to follow the yuan Liang Jin under relatively normal conditions: because of the rapid sound! Pathogenic bacteria, small cattle farm umbrella cabinet (do inside the recognition line 4.2 please! Before, the wet martial arts method to clean the meeting surface and the room with the tower, and then with the outside of the device or other methods to disinfect the inside of the device,
13 buy the virtual test 1 made, "single, constant, in, enter for the bacteria age, the wind adds the total evaluation into the experiment prison. Of course, the actual wear nuclear movement from the clothes, busy results, 1 three,
4.4 to be collected makeup Europe did not become more collected yuan period report management, shock to the degree in the expansion of 1 complete fire surface, square! Times fast film: , 5: small show of consideration, car points in the city instrument health with the city think will be analyzed and other water or cloud home water or when the source is important six.6 is the product of health: women's shock water, phosphate juice filter, grinding culture medium, bovine positive potential protein, standard hard water, bovine: longer bacteria reduction.
47 large non-village agent: before use should check the content of the package surface intact let: there is no damage when avoid use. 4.8 The reagents and accompanying materials of the factory should not be brought in at a certain time. 4.9 The liquid or reagents should be dialed at the appropriate time. The test sequence can be verified by: 11. The clean liquid and the type of equipment should be paid attention to. Several people should be placed in the virtual container for disinfection. 4.1 The pressure test method should not be carried out on the objects. The microbial contamination rate should not be quickly determined. Regardless of whether it is toxic, the indoor air can be treated with a virtual air flow control plan after the whole test. 5 samples in English
samples have good Zhi 4: the topic of a small room in a box of random selection of 12 most popular columns, its +4 steam samples, 4 pieces of Russian. Take the soft leisure can reduce the 4 heart transmission stable attention fierce into the sample of the product small gold collection of the broken package, strange before the inspection do not consider the rise,
6 chemical products washing south, buckle to the effectiveness of the evaluation principle! The method of killing the purchase! The method of the work of the product is the standard of the product. The method of the product is the product of the product. The product is the product of the product! 6.3 inspection with the indicator microorganism
production market execution of the original control of the party non-Sichuan Shangxing Zhi! In the test of the reduction (home or provincial window management store: wide light test fabric, also 1 unveiled production of ectopic works store owner industry front rush as a test bacteria. Read the South of the name
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Table 1 Anti-, the letter of the daily chemical product test of the selection of microorganisms
ATCX $$J: ATCC 2TZ17
S[190 ATY.C 2S922
According to the strict requirements of the environment indicated by the period,
3.5 work time
received 5 explicit requirements for the cost of the:
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QB/27332905
Kenan, welcome north product month
show, skin peptide membrane, and a section will stand
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can test about half of the results are the results of the test and the results of the method, the result report includes: al
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work requirements:
allowed use:
effect evaluation results:
determination of the living fruit has an impact on other factors.
Test methods for antibacterial and antimicrobial effects of daily chemical products Classification of test methods for antibacterial effects of chemical products Table 2.
Test methods for antibacterial effects of tea shade test Methods ... Kea-
type product
and type identification sea wing method training series
inhibit repair co-operation skin peptide cleaning product
anti-corrosion type oral chemical product company
inhibit type type cleaning paint treatment
rice source standard
Qn/T 27382005
maintain pH L:
no tester,
no degree of suction convex (1.0ml, 5.0mL, 10.0mL) temperature incubator:
counter
essence:
automatic mixer;
constant mixing box:
water overflow deep pot:
heat sterilization pot:
seven on the balance:
biological safety national clean microbiological test necessary instruments. 7.22 Reagents
Nutrient agar medium
Weigh 10.0g egg whites, 5.0g beef, 5.0g mouse embryos, add 1000mL distilled water to dissolve, adjust pH to 7.27.4, then add 15.Ug agar to dissolve, divide into packages, sterilize at 121℃ pressure steam for 20min, and set aside for use in agar culture
Weigh 40.0g yellow mulberry: 10.0g egg white, 20.0g celery: add 1000mL distilled water, mix the above ingredients, heat to dissolve, adjust pH To 15.6 ± (2:115 ° C [K force steam sterilization 30min reserve month)
Limit knockout buffer (PHS): 003m/) Weigh 2.83g of anhydrous disodium phosphate and 1.36g of sodium hyaluronate, heat them to 10mL, and after complete decomposition, adjust the pH to 7.2-7.4, sterilize at 121°C for 20min and reserve: d
Standard water (magnetic 342 degrees)
Weigh 0.034g of bottled chemical (C.aCl) and 0.139g of magnesium chloride (MgC16H0), add 1000mL of distilled water, sterilize with 1217 pressure steam in 20mm container.
c) Neutralizing agent:
1) No. 5 thiourea, 1000mL of distilled water: 1.36g of phosphoric acid and 1.36g of hydrogen peroxide. 6. Phosphoric acid 2.83g monophosphate 10.0g, glycine 10.0g, 30.0g temperature 80, 2
distilled water 1000 jin;
potassium dihydrogen phosphate 1.3hg, 2.83g of flammable acid, 1 phospholipid 3.0g, 20Ug tween 80, steamed water 100ml.3
4): 20.0g Tween, acid substitution 0.0, PRs1Mm. , use 1 type supply, 2, 3 for non-nitrogen type collection agent: 4 Le Sui. (1) Preparation of bacteria 1) Take a frozen seed tube, open it without any operation, and use a fine pipette to insert the live culture medium. Gently blow and suck several times to melt and disperse the bacteria. Take 5.0mL of clean nutrient medium and drop a small amount of bacteria into it. Incubate at 37℃ for 13h~24h. Use the remaining amount of the culture medium to streak on a nutrient medium plate and incubate at 37℃ for 18h~24h. Drain the typical extract of the second step and inoculate it with dry nutrient blood, and culture it for 18h~24h to get the third generation culture medium: Figure 3-14 generation nutrient culture medium (13h24), use a 5.0mL pipette to pipette 3.05.0mL of the suspension into a sterile tube, absorb the hydrogen, wash the bacteria, then use a 5.0mL pipette to transfer the washed liquid to a single bacterial test tube, and mix it by dynamic mixing (205 °C) or by shaking on a microscope to make the bacteria as evenly dispersed as possible:
The prepared bacterial suspension should first be roughly measured by cell concentration determination method, and then the diluted solution should be sieved to the desired concentration
4) The bacterial suspension should be stored in a refrigerator for use. Do not use blue, do not leave overnight: 5) When there is contamination, it should be identified by seedling staining, blue staining and chemical test, (2) Prepare carbon fiber (stereochromic) wave, open with bacteria, use a fine pipette to aspirate the sand in the tube, gently aspirate several times, so that the whole can be separated, collect the sand solution for culture, although 5.0m10.0 test tube, fill with a little bacteria flushing liquid, once 37124. Use a long ring double-cell growth liquid, must be directly flushed on the sand plate: T37 emperor all 18h~24h, take the above second generation of culture material: even the sand material for the time, 7-7 culture 18h--24h, 4 for the third culture. Store it at 4, the appropriate time should be no less than 6 weeks; 2) During the test: collect the 3rd generation culture material and continuously subculture it on the sand surface, the method is the same as the 3rd generation, the 5th and 6th generation materials are selected and cultured on the new surface for 18h~24h (3.0ml-5.0ml of the obtained solution is added to the test material, and the bacteria are removed. Then use 5.0ml to temporarily transfer the solution to Another test tube towel, use the electric device to wet the table for 20s, or spread it on the school 1. Vibration system for students! Color bed South Chun Shu B:
sharp lips pale prompt in the heart of the box should be continued, hands overnight: suspected of contamination, should be identified by South extraction concept, Blanche gas and chemical test methods: the first economic morphology can be directly observed by microscopy. The South body morphology can be written on the smear and cited by the case microscope. It can also be dyed with a negative constant! The bacteria are moistened with black technology, and then observed after reduction. 7.2.3 Neutralizer testing method
In order to accurately measure the killing effect of the microorganisms, it is required to add a suitable "neutralizer" in the killing test. The neutralizer can not only stop the effect of the anti-washing agent on the microorganisms in time, but also the reaction product (i.e., the neutralizer) of the test product has no inhibitory or killing effect on the microorganisms. 7.2.3.1 Neutralizer identification test
According to the 3 points ranking.
Table 3 Neutralizer identification test
Conform to the special policy to reduce 0.5
People enter
Total value = nT.)
Allow 10min shop, take the original
middle board (2 pieces/sample!
rotten product 50
PRS 1.Sml.
Appropriate correction
product 5.0mL4 product.0L5.mT.
Permeate
PI5S 4 5 n-1.
Appropriate will
neutralize a5.m
naturally, adjust: add 372 culture 48, calculate the south fall, calculate the collection number according to the heat sample multiple l. virtual base concentration table preparation: mPBS is washed green and made into 1×efw/m9×1refuml. liquid and product are: test the whole selection 1.. and cattle swim no! :. steam, m reduction TTKAN KAa
QB,T 27332CC5
Neutralizer license price regulations
Build 1 new long-term significant safety lower grade 2:
The first group of medical efficacy is significantly less than the first, 4, 5 standards
, 4, 5 numbers are similar, +, 1 empirical theft public%:
The sixth average yuan is the same as the cattle,
e) three extension and half period number (the first number of the third group + the fourth red country whole + the fifth group of southern children) +3: the period is good south line No. 84+single 5 within 4+3169
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The above evaluation of the neutralizer table can be summarized except for the test whole single index, the birth month of the grape, the neutralization, the neutralization of the age group a table index, through no other main, the standard is the language consultation standard product profit production emulsion. 1. 2.4 Killing test: BS liquid test bacteria number [72.2f you format: fee request table: Yi 0m control source (PHS151.mla
, the amount of water collected is ×umL-×eitmL:
the total risk rod product is useless, the standard amount of water is taken from the cup to the product concentration center a
micro-relative total original liquid is 50. Respectfully enter the single test report, 20C flow m type product take the test delivery me 01 add people containing 5.0m in the tube, speed product uniform, stand: d
work! After this time: select the overflow bacteria and sample 0.1ml into the 5.m, after the fire proof tube, now:
10mr: the sample solution is properly diluted, collect 2 weak group liquid 1a, 100 t)
Yingxing World Fire: Each column or the two benefits of the city on the Beijing West 405 center of the shell should be lipid culture parent (red lipid culture production seat note: Zhi points, environment level, 352) 4 () 72), number:
to replace the sample, the time and intensity above Luo resources, as a reference! : h repeated once change the average depth should be
7.2 5 Calculation formula:
For the original sample average smart number:
The number of falling lights in the inspection room,
The number of fruit grids
The error evaluation of 2.6 live bacteria count
Kill data Shenzhen)
fE×100
The correction rate of the average error is 10%, and the empty rate can be checked. According to x23 generation <7)
the average number of colonies between plates and the average number of blue colonies between plates, the average number of colonies between plates, the total number of colonies between plates, the average number of base numbers between plates, the average type x100
the average bacterial density between plates:
the average bacterial density between three plates
the correct number of colonies between plates. OH/T 273R2005
(with the average number of devices-year new sample points) absolute five conversion number
ene selectivity judgment capital design case one
7.2.7 Antibacterial effect evaluation of daily chemical products of antibacterial type by dilution method
carving degree
Table 4 Antibacterial effect evaluation
Effect from production and study
Work time/:
"King's life
(90:-50)
One take, for many and the heart of life, I run teaching, 1.3 Antibacterial effect test method of daily chemical products of antibacterial type: 7.31 Equipment
1.32 Test preparation
-1 7.2.2.
, 37 reports, supplementary test steps
Bs reverse the shade island 7.2 2 f:1 ratio when the new release water concentration is: take 0.1 m:. concentrated in the column selected liquid1 dilution process, the required concentration is /×0cfu/ml.~×10m, then add the cattle and clean: protein solution (3%): d) Preparation of staining slides
Each slide (7.4.2.1) is connected to 20μ total wave, placed in culture medium, covered, and placed in a (35=2) incubator for 20min:
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