Guidelines for the conduct of tests for distinctness,uniformity and stability-Bermudagrass
Some standard content:
ICS65.020.20
Agricultural Industry Standard of the People's Republic of China
NY/T2491—2013
Guidelines for the conduct of tests for distinctness , uniformity and stability-Bermudagrass
[Cynodon dactyton(L.)Pers. andxCynodon dactylon(L.)Pers.Cynodon transvalensis Burtt-Davyl2013-12-13Issued
2014-04:01Implementation
Ministry of Agriculture of the People's Republic of China
Normative references
Terms and definitions
Requirements for propagation material
Test methods
Determination of specificity, consistency and stability of results 8 Character table
9 Grouped characters
10 Technical questionnaire·
Appendix A (Normative Appendix) Character table of bermudagrass Appendix B (Normative Appendix) Interpretation of the character table of bermudagrass Appendix (Normative Appendix)
Format of technical questionnaire on bermudagrass
XY/T 2491--2013
NY/T 2491—2013
This standard is based on the rules given in GB/T 1.12C09. This standard was proposed by the Seed Administration Bureau of the Ministry of Agriculture: This standard was proposed by the National Technical Committee for Standardization of New Plant Varieties Testing (SAC/T: 277) and belongs to 11. The initiators of this standard are: South China Agricultural University, Science and Technology Development Center of the Ministry of Agriculture, and the main initiators of this standard are: Rao Dehua, Ren Yonghao, Zhang Juming, Xu Zhenjiang, Wan Hong, Lang Youla. 1 Scope
NY/T 2491—2013
Guidelines for the specificity, consistency and stability testing of new plant varieties Cynodon dactyon
This standard specifies the technical requirements and general principles for the results of the specificity, consistency and stability testing of new varieties of Cynodon dactyon. This standard is applicable to common Cynodon dactyon (l.) Per.-, hybrid Cynodon dactyon [Cynodus dactyon (l.) Pers. × Cynodus translensis Burt-Davy new variety specificity, consistency and stability test and result judgment:
2 Normative references
The following documents are indispensable to the preparation of this document: For any referenced document with a date, only the version with the date shall apply to this document. For any referenced document without a date, the latest version (including all amendments) shall apply to this document. (B/T19557.1 General Guidelines for Specificity, Consistency and Stability Tests for New Plants 3 Terms and definitions
The terms and definitions defined in GB/T19557.1 shall apply to this document. 3.1
Group measurement of a group of plants or parts uf plants Measurement of a certain organ or part of a flowering plant or plant to obtain a record of a group 3.2
Individual measurement measurement uf a number of individual plants ur parts uf 3.3 Visual assessment by a single observation of a group of plants or parts 3.4 Visual assessment by observation of individual plants or parts Plants individual visual inspection
Visual inspection of the plant or a certain organ or part of the plant is carried out one by one, and the individual records are obtained. 4 Symbols
-The following symbols are applicable to this document:
MG: Group measurement.
MS: Individual measurement.
VG Group visual inspection.
VS: Individual visual inspection:
Quality trait.
QN: Quantitative trait.
PQ: Pseudo-quality trait.
(a) (): The content of the mark is explained in detail in B,2>Y/T2491—2013
(1 The content of the mark is explained in detail in.3. A: Indicates that the trait is tested under the pit planting method. B: Indicates that the trait is tested under the row planting method. 5 Requirements for propagation materials
5.1 Propagation materials are provided in the form of seeds or nutrient tea. 5.2 If the propagation materials submitted are seeds, the number of seeds shall be at least 200; if the propagation materials submitted are vegetative stems, the number of vegetative stems shall be at least 300, and each plant shall contain 2 nodes.
5.3 The propagation materials submitted shall be healthy, vigorous and disease-free. The specific quality requirements for the seed propagation materials submitted are: germination rate 5%, purity 95.0%, and water content 11.0%. 5.4 The propagation materials submitted shall generally not be treated in any way that affects the expression of variety traits. If they have been treated, detailed descriptions of the treatment shall be provided. 5.5 The propagation materials submitted shall comply with the relevant provisions of China's plant quarantine. 6 Test methods
6.1 Test cycle
The test period shall be at least 2 independent growth cycles: the complete growth process of Bermuda grass. For seed propagation, the test period refers to the germination, seedling growth, berberination, flowering and fruiting, and the period from seed sowing to seed? until maturity; for vegetative propagation, it refers to the entire growth period from the beginning of flowering and fruiting to seed maturity after vegetative cuttings. 6.2 Test location
The test is usually carried out at a location. If some traits cannot be fully expressed at this location, they can be observed at other locations that meet the conditions.
6.3 Field test
6.3.1 Test design
Please plant varieties and similar varieties adjacently.
Set up two test plots: pit sowing and strip sowing. Planting in pit sowing: > The row spacing of each plot is 200e1×200cm, and each plot of seed propagation materials is at least 20 palms, and each plot of vegetative stem propagation materials is at least 15 plants. There are 2 replications in total. Planting in strips. The plot is 2m2, the sowing rate of seed propagation materials is 1/㎡, and the row spacing of vegetative stem propagation materials is cmx15cm; there are 2 replications in total.
6.3.2 Field management
Follow the “local cattle production management method”
6.4 Trait observation
6.4.1 Observation period
Trait observation should be carried out according to the small cattle breeding stages in Table A. and Table A.2: Appendix B explains these breeding stages. 6.4.2 Observation method
Trait observation should be carried out according to the observation method (VC, VS, MC, MS) specified in Table A.1 and Table A.2. 6.4.3 Number of observations
Unless otherwise specified. Individual observation traits (VS, MS strain sampling standard Standard number: 10. When observing organs or parts of implants, the number of samples taken from each plant should be 1. Group observation traits (VG, MG) should observe the entire plot or a mixed sample of a specified number of people. 6.5 Additional tests
If necessary, the traits in Table A.2 or traits not listed in this document can be selected for additional tests 2
7 Determination of specificity, consistency and stability results 7.1 General principles
The determination of specificity, consistency and stability shall be carried out in accordance with the principles determined in B/T19557.1. 7.2 Determination of specificity
NY/T 2491—2013
The proposed variety should be clearly distinguishable from all known varieties. When the proposed variety has obvious and reproducible differences from similar varieties in at least one trait during the test, the proposed variety can be considered to be specific. 7.3 Determination of consistency
For Bermuda grass varieties, when determining specificity, the degree of variation of the variety cannot significantly exceed that of the solid type variety. 7.4 Determination of stability
If the variety is consistent, it can be considered that the variety is stable. Generally, no stability test is performed. If necessary, the next generation seeds of the variety can be planted. Compared with the previously provided propagation materials, if there is no obvious change in trait expression, the variety can be determined to be stable.
8 Trait expression
According to the test needs,The traits are divided into basic traits and optional traits. Basic traits are the traits that must be used in the test. Table A.1 lists the basic traits of bermudagrass. Table A.2 lists the optional traits. 8.1 Overview
The trait table lists the name, expression type, expression state and corresponding code and standard variety, observation period and method, etc. 8.2 Expression type
According to the expression method of the trait, the traits are divided into three types: quality traits, pseudo-quality traits and quantitative traits. 8.3 Expression state and corresponding code
8.3.1 Each trait is divided into a series of expression states. In order to define the trait and standardize the description, each expression state is assigned a corresponding digital code to facilitate data recording, processing and the establishment and communication of variety descriptions. 8.3.2 For quality traits and pseudo-quality traits, all expression states should be listed in the test guide: For quantitative traits, in order to shorten the length of the trait, the expression states of even-numbered codes can be omitted. The expression states of even-numbered codes can be described as the form of the previous expression state to the next expression state.
8.4 Standard varieties
The trait table lists the standard varieties with corresponding expression states of some traits to help identify the different expression states of the same trait. and correct for differences caused by year and location,
9 Grouped traits
In this document, the grouped traits of varieties are as follows:) Ploidy Table A.1 Neutral trait 1)
h) Plant: Natural height (Table A.1 Neutral trait 3): Flower: Stigma color (Table A.1 Trait 16) d) Flowering period (Table A.1 Neutral trait 20),
10 Technical questionnaire
The applicant should fill in the Bermudagrass Technical Questionnaire NY/T 2491—2013 according to the format given in Appendix (tt||A.1 Basic characteristics of bermudagrass
See Table A.1
Please note that the growth is based on the plant: natural height
Controlled: internode length
Controlled, diameter
Leaf limit: normal color
Optional leaf blade: old color
Print: red
Appendix A
【Normative record】
Character table of bermudagrass
Basic characteristics table of bermudagrass
Replacement time and method
Expression status
Triploid
"Ploid
Back to Ploid
Half-true
Wet green
Gray green
No or very accurate
New label
Tildlwert
Tilalweaur
Fifspo't
Tiigrten
TEtwari
Double flower dog tooth liquid
Jifway
Tifgreen
Tifway||t t||Tingreer
Tifuruten
Ice Duyouya
Tifgreen
Leaf width}
Reproductive branch: cyan color at nodes
Inflorescence length
Inflorescence: dense
Spike: green degree of spike axis
Spikelet: number of florets
Flotlet: stigma color
Flotlet: anther color
Outer glume: glume base anthocyanin color
Outer glume: glume tip flower color| |tt||"Blooming period
A.2 Selected traits of bermudagrass
See Table A.2.
Base: length
Table A.1 (continued)
Observation period and method
Expression state
Light purple
Dark purple
Light blue
Purple-red at the tip
Light purple
Purple-red
Table of selected traits of bermudagrass
Observation period and strength:
Decoration state
NY/T 2491-2013
Standard variety
Tifgreen
Suzhi Dogtooth
Tifgreen
ifdwar:
Su Dogtooth
Tifeag'e
Double flower weak tooth version
Titway
Double flower Dogtooth
Tif way
Tifeagle
Tifdwa:f
Tilway
Tifgreen
ifdwar:
Tifsrt
Standard variety
Tifdwarl
Tifgten
NY/T 2491—2013
Thunder control: Fish
Resistance to scurvy
Resistance to rust
Table 42 (continued)
Double test results and methods
Expression status
Brown color
Standard sample dynamic
ifgrecn
13.1 Growth stage of bermudagrass
See Table B.1|| tt||Appendix B
【Normative Appendix】
Explanation of the table of Bermuda grass traits
Table B.1 Table of growth stages of Bermuda grass
Wei Meng long-term, large-scale expansion
Pregnancy period, large-scale flowering
Hanging period.0 Plant treatment note
Standard period, no flowering
B.2 Explanation of multiple traits
NY/T 2491—2013
The observation position of each generation is the longest primary stem, and its internode position is the first fully developed node. The leaf observation position is the expanded leaf in the middle of the true stem: the observation position of the spike is the spikelet in the middle of the main flowering spike of the plant. B.3 Explanation of individual traits
The trait classification and the code in the figure are shown in Table A.! : Character 2 Plant; Growth habit. Figure 1
Semi-management
Plant: Growth habit
Dou shape 3 Plant: Natural height, incubation period, natural height of the most erect stem of the plant. NY/T2491—2013
Character 4, runner: internode length, see Figure B.2. Internode length
Figure B2 Runner length and internode length
Character 7 Leaf: Upper wheat surface hair, see Figure B.2 None or very sparse
Figure B.3 Leaf: Upper surface hair
Character 8 Leaf: Leaf sheath hair, see Figure 13.1. None
Figure B.4Leaf: sheath hairs
Figure bamboo
Leaf: ligule cilia. See Figure B.5.
Figure B.5Leaf: ligule cilia
Characteristics 11.Reproductive branches: internodes anthocyanin color, see Figure B.6, None
Reproductive branches: internodes anthocyanin color
Characteristics 12Inflorescence: length, see Figure B.7. Length of compound spike inflorescence
Length of compound spike inflorescence
Figure B.7Length and width of inflorescence
NY/T2491-23131 Character 16) d) Flowering period (Table A, 1 Character 20),
10 Technical Questionnaire
The applicant should fill in the Bermudagrass Technical Questionnaire NY/T 2491—2013 according to the format given in the Appendix (tt||A.1 Basic characteristics of bermudagrass
See Table A.1
Please note that the growth is based on the plant: natural height
Controlled: internode length
Controlled, diameter
Leaf limit: normal color
Optional leaf blade: old color
Print: red
Appendix A
【Normative record】
Character table of bermudagrass
Basic characteristics table of bermudagrass
Replacement time and method
Expression status
Triploid
"Ploid
Back to Ploid
Half-true
Wet green
Gray green
No or very accurate
New label
Tildlwert
Tilalweaur
Fifspo't
Tiigrten
TEtwari
Double flower dog tooth liquid
Jifway
Tifgreen
Tifway||t t||Tingreer
Tifuruten
Ice Duyouya
Tifgreen
Leaf width}
Reproductive branch: cyan color at nodes
Inflorescence length
Inflorescence: dense
Spike: green degree of spike axis
Spikelet: number of florets
Flotlet: stigma color
Flotlet: anther color
Outer glume: glume base anthocyanin color
Outer glume: glume tip flower color| |tt||"Blooming period
A.2 Selected traits of bermudagrass
See Table A.2.
Base: length
Table A.1 (continued)
Observation period and method
Expression state
Light purple
Dark purple
Light blue
Purple-red at the tip
Light purple
Purple-red
Table of selected traits of bermudagrass
Observation period and strength:
Decoration state
NY/T 2491-2013
Standard variety
Tifgreen
Suzhi Dogtooth
Tifgreen
ifdwar:
Su Dogtooth
Tifeag'e
Double flower weak tooth version
Titway
Double flower Dogtooth
Tif way
Tifeagle
Tifdwa:f
Tilway
Tifgreen
ifdwar:
Tifsrt
Standard variety
Tifdwarl
Tifgten
NY/T 2491—2013
Thunder control: Fish
Resistance to scurvy
Resistance to rust
Table 42 (continued)
Double test results and methods
Expression status
Brown color
Standard sample dynamic
ifgrecn
13.1 Growth stage of bermudagrass
See Table B.1|| tt||Appendix B
【Normative Appendix】
Explanation of the table of Bermuda grass traits
Table B.1 Table of growth stages of Bermuda grass
Wei Meng long-term, large-scale expansion
Pregnancy period, large-scale flowering
Hanging period.0 Plant treatment note
Standard period, no flowering
B.2 Explanation of multiple traits
NY/T 2491—2013
The observation position of each generation is the longest primary stem, and its internode position is the first fully developed node. The leaf observation position is the expanded leaf in the middle of the true stem: the observation position of the spike is the spikelet in the middle of the main flowering spike of the plant. B.3 Explanation of individual traits
The trait classification and the code in the figure are shown in Table A.! : Character 2 Plant; Growth habit. Figure 1
Semi-management
Plant: Growth habit
Dou shape 3 Plant: Natural height, incubation period, natural height of the most erect stem of the plant. NY/T2491—2013
Character 4, runner: internode length, see Figure B.2. Internode length
Figure B2 Runner length and internode length
Character 7 Leaf: Upper wheat surface hair, see Figure B.2 None or very sparse
Figure B.3 Leaf: Upper surface hair
Character 8 Leaf: Leaf sheath hair, see Figure 13.1. None
Figure B.4Leaf: sheath hairs
Figure bamboo
Leaf: ligule cilia. See Figure B.5.
Figure B.5Leaf: ligule cilia
Characteristics 11.Reproductive branches: internodes anthocyanin color, see Figure B.6, None
Reproductive branches: internodes anthocyanin color
Characteristics 12Inflorescence: length, see Figure B.7. Length of compound spike inflorescence
Length of compound spike inflorescence
Figure B.7Length and width of inflorescence
NY/T2491-23131 Character 16) d) Flowering period (Table A, 1 Character 20),
10 Technical Questionnaire
The applicant should fill in the Bermudagrass Technical Questionnaire NY/T 2491—2013 according to the format given in the Appendix (tt||A.1 Basic characteristics of bermudagrass
See Table A.1
Please note that the growth is based on the plant: natural height
Controlled: internode length
Controlled, diameter
Leaf limit: normal color
Optional leaf blade: old color
Print: red
Appendix A
【Normative record】
Character table of bermudagrass
Basic characteristics table of bermudagrass
Replacement time and method
Expression status
Triploid
"Ploid
Back to Ploid
Half-true
Wet green
Gray-green
No or extremely accurate
New label
Tildlwert
Tilalweaur
Fifspo't
Tiigrten
TEtwari
Double flower dog tooth liquid
Jifway
Tifgreen
Tifway||t t||Tingreer
Tifuruten
Ice Duyouya
Tifgreen
Leaf width}
Reproductive branch: cyan color at nodes
Inflorescence length
Inflorescence: dense
Spike: green degree of spike axis
Spikelet: number of florets
Flotlet: stigma color
Flotlet: anther color
Outer glume: glume base anthocyanin color
Outer glume: glume tip flower color| |tt||"Blooming period
A.2 Selected traits of bermudagrass
See Table A.2.
Base: length
Table A.1 (continued)
Observation period and method
Expression state
Light purple
Dark purple
Light blue
Purple-red at the tip
Light purple
Purple-red
Table of selected traits of bermudagrass
Observation period and strength:
Decoration state
NY/T 2491-2013
Standard variety
Tifgreen
Suzhi Dogtooth
Tifgreen
ifdwar:
Su Dogtooth
Tifeag'e
Double flower weak tooth version
Titway
Double flower Dogtooth
Tif way
Tifeagle
Tifdwa:f
Tilway
Tifgreen
ifdwar:
Tifsrt
Standard variety
Tifdwarl
Tifgten
NY/T 2491—2013
Thunder control: Fish
Resistance to scurvy
Resistance to rust
Table 42 (continued)
Double test results and methods
Expression status
Brown color
Standard sample dynamic
ifgrecn
13.1 Growth stage of bermudagrass
See Table B.1|| tt||Appendix B
【Normative Appendix】
Explanation of the table of Bermuda grass traits
Table B.1 Table of growth stages of Bermuda grass
Wei Meng long period, large piece expansion strict
Pregnancy period, large flower
Hanging ear period.0 Plant treatment note
Maturity period, 0 No forest flowering
B.2 Explanation of multiple traits
NY/T 2491—2013
The observation position of each generation is the longest primary stem, and its internode position is the first fully developed node. The leaf observation position is the expanded leaf in the middle of the true erect stem: the observation position of the ear is the spikelet in the middle of the main flowering ear of the plant. B.3 Explanation of single traits
The trait classification and the code in the figure are shown in Table A.! : Character 2 Plant; Growth habit. Figure 1
Semi-management
Plant: Growth habit
Dou shape 3 Plant: Natural height, incubation period, natural height of the most erect stem of the plant. NY/T2491—2013
Character 4, runner: internode length, see Figure B.2. Internode length
Figure B2 Runner length and internode length
Character 7 Leaf: Upper wheat surface hair, see Figure B.2 None or very sparse
Figure B.3 Leaf: Upper surface hair
Character 8 Leaf: Leaf sheath hair, see Figure 13.1. None
Figure B.4Leaf: sheath hairs
Figure bamboo
Leaf: ligule cilia. See Figure B.5.
Figure B.5Leaf: ligule cilia
Characteristics 11.Reproductive branches: internodes anthocyanin color, see Figure B.6, None
Reproductive branches: internodes anthocyanin color
Characteristics 12Inflorescence: length, see Figure B.7. Length of compound spike inflorescence
Length of compound spike inflorescence
Figure B.7Length and width of inflorescence
NY/T2491-2313
Tifsrt
Standard variety
Tifdwarl
Tifgten
NY/T 2491—2013
Yu Lei Service: Fa Xi Dun Fish
Resistance to ridge reduction and death
Resistance to rust
Table 42 (continued)
Double test performance and method
Expression status
Brown color
Standard variety
ifgrecn
13.1 Growth stage of Bermuda grass
See Table B.1|| tt||Appendix B
【Normative Appendix】
Explanation of the table of Bermuda grass traits
Table B.1 Table of growth stages of Bermuda grass
Wei Meng long-term, large-scale expansion
Pregnancy period, large-scale flowering
Hanging period.0 Plant treatment note
Standard period, no flowering
B.2 Explanation of multiple traits
NY/T 2491—2013
The observation position of each generation is the longest primary stem, and its internode position is the first fully developed node. The leaf observation position is the expanded leaf in the middle of the true stem: the observation position of the spike is the spikelet in the middle of the main flowering spike of the plant. B.3 Explanation of individual traits
The trait classification and the code in the figure are shown in Table A.! : Character 2 Plant; Growth habit. Figure 1
Semi-management
Plant: Growth habit
Dou shape 3 Plant: Natural height, incubation period, natural height of the most erect stem of the plant. NY/T2491—2013
Character 4, runner: internode length, see Figure B.2. Internode length
Figure B2 Runner length and internode length
Character 7 Leaf: Upper wheat surface hair, see Figure B.2 None or very sparseWww.bzxZ.net
Figure B.3 Leaf: Upper surface hair
Character 8 Leaf: Leaf sheath hair, see Figure 13.1. None
Figure B.4Leaf: sheath hairs
Figure bamboo
Leaf: ligule cilia. See Figure B.5.
Figure B.5Leaf: ligule cilia
Characteristics 11.Reproductive branches: internodes anthocyanin color, see Figure B.6, None
Reproductive branches: internodes anthocyanin color
Characteristics 12Inflorescence: length, see Figure B.7. Length of compound spike inflorescence
Length of compound spike inflorescence
Figure B.7Length and width of inflorescence
NY/T2491-2313
Tifsrt
Standard variety
Tifdwarl
Tifgten
NY/T 2491—2013
Yu Lei Service: Fa Xi Dun Fish
Resistance to ridge reduction and death
Resistance to rust
Table 42 (continued)
Double test performance and method
Expression status
Brown color
Standard variety
ifgrecn
13.1 Growth stage of Bermuda grass
See Table B.1|| tt||Appendix B
【Normative Appendix】
Explanation of the table of Bermuda grass traits
Table B.1 Table of growth stages of Bermuda grass
Wei Meng long-term, large-scale expansion
Pregnancy period, large-scale flowering
Hanging period.0 Plant treatment note
Standard period, no flowering
B.2 Explanation of multiple traits
NY/T 2491—2013
The observation position of each generation is the longest primary stem, and its internode position is the first fully developed node. The leaf observation position is the expanded leaf in the middle of the true stem: the observation position of the spike is the spikelet in the middle of the main flowering spike of the plant. B.3 Explanation of individual traits
The trait classification and the code in the figure are shown in Table A.! : Character 2 Plant; Growth habit. Figure 1
Semi-management
Plant: Growth habit
Dou shape 3 Plant: Natural height, incubation period, natural height of the most erect stem of the plant. NY/T2491—2013
Character 4, runner: internode length, see Figure B.2. Internode length
Figure B2 Runner length and internode length
Character 7 Leaf: Upper wheat surface hair, see Figure B.2 None or very sparse
Figure B.3 Leaf: Upper surface hair
Character 8 Leaf: Leaf sheath hair, see Figure 13.1. None
Figure B.4Leaf: sheath hairs
Figure bamboo
Leaf: ligule cilia. See Figure B.5.
Figure B.5Leaf: ligule cilia
Characteristics 11.Reproductive branches: internodes anthocyanin color, see Figure B.6, None
Reproductive branches: internodes anthocyanin color
Characteristics 12Inflorescence: length, see Figure B.7. Length of compound spike inflorescence
Length of compound spike inflorescence
Figure B.7Length and width of inflorescence
NY/T2491-2313
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