Some standard content:
Appendix A, Appendix C, Appendix D and Appendix E of this standard are normative appendices, and Appendix B is an informative appendix. This standard was proposed by the Agricultural Reclamation Bureau of the Ministry of Agriculture.
This standard is under the jurisdiction of the Technical Committee for Standardization of Tropical Crops and Products of the Ministry of Agriculture. NY/T689--2003
The drafting units of this standard are: Tropical Horticulture Research Institute of Chinese Academy of Tropical Agricultural Sciences and College of Horticulture of South China University of Tropical Agriculture. The main drafters of this standard are: Wang Jiabao, Chen Yeyuan, Deng Suisheng, Li Shaopeng and Wei Shouxing. I
1 Scope
Guava grafted seedlings
NY/T 689-—2003
This standard specifies the requirements, test methods, inspection rules, packaging, marking, storage and transportation of guava (Psidium guajava L.) grafted seedlings.
This standard applies to guava grafted seedlings. 2 Normative references
The clauses in the following documents become the clauses of this standard through reference in this standard. For dated references, all subsequent amendments (excluding errata) or revisions are not applicable to this standard. However, parties to agreements based on this standard are encouraged to study whether the latest versions of these documents can be used. For undated references, the latest versions apply to this standard. GB9847 Apple seedlings
GB15569 Quarantine regulations for agricultural plant transportation
Regulations on Plant Quarantine (State Council of the People's Republic of China) Implementation Rules of the Regulations on Plant Quarantine (Agricultural Part) (Ministry of Agriculture of the People's Republic of China) 3 Terms and definitions
The following terms and definitions apply to this standard. 3.1
Height of graft-union The distance from the soil surface or ground to the grafting point.
Diameter of grafting The thickest diameter 3cm above the grafting point. 3.3
diameter of lateral rootThe average diameter of the lateral root of the stock at 2 cm from the base. 3.4
number of lateral rootThe number of lateral roots with a diameter greater than 0.1 cm growing from the main root of the stock. 3.5
seedling growing in bagSeedlings grown in polyethylene nutrient bags or other containers of certain specifications filled with nutrient soil. 3.6
seedling growing in fieldSeedlings grown and propagated in the field.
seeding with naked rootsSeedlings whose roots are not covered with soil when they are planted. 1
NY/T 689—2003
purity of variety
The proportion of seedlings with the same characteristics of the same variety, expressed as the percentage of the number of seedlings of this variety to the number of seedlings for inspection. 4 Requirements
4.1 Rootstock
4.1.1 Adapt to local climate conditions and have strong resistance to adversity. 4.1.2 Strong grafting affinity with scion varieties, high grafting survival rate, and consistent growth. 4.1.3 Strong growth, well-developed root system, and no quarantine pests and diseases. 4.1.4 Do not use old, weak, or diseased rootstocks. 4.1.5 Rich seed sources, convenient and easy to obtain.
4.1.6 During the rootstock breeding process, there is no root-knot nematode damage in its growth environment. 4.1.7 It is recommended to use this variety of rootstock.
4.2 Scion
4.2.1 The scion must come from a confirmed pure variety, a high-quality and high-yielding mother garden or mother plant. 4.2.2 The new shoots of the current year that are free of pests and diseases, have full growth, full buds, and fully mature leaves should be selected as scions. The stem tips and leaves of the new shoots should be removed 5 to 7 days before the scion is harvested to make the buds full. 4.2.3 When introducing scions from other places, in addition to strictly requiring the pure variety, they should also be inspected by the quarantine department and can only be introduced after obtaining the "Plant Quarantine Certificate".
4.2.4 Recommended scion varieties include: Pearl, Seedless, New Century, Rouge and other guava varieties. 4.3 Grafting methods
Grafting methods such as cutting grafting, bud grafting, and belly grafting can be used. 4.4 Quality requirements
4.4.1 Basic requirements
Degree of healing of grafted joint: smooth and well healed, without bulges or tumor-like swellings, and no strangulation after removing the binding bands; height of grafted seedlings ≥45cm;
Growth of grafted seedlings: normal and strong growth, green leaves with a slight luster; Pests and diseases: no pests and diseases such as wireworms occur at the roots, and there are no quarantine pests and diseases on the stems, branches and leaves. The number of leaves with various diseases does not exceed 20% of the total number of leaves of a single plant, and diseased leaves are not allowed to grow in the seedlings. The seedlings should be disinfected when they are planted, and there should be no disease spots on the stems and branches; bagged seedlings: the diameter of the soil ball is >15cm, the height is 20cm, the seedling bag is not seriously damaged, and the soil ball is not loose; bare-root seedlings: the main root and the primary lateral roots on the main root are longer than 20cm, the roots are slurried with mud, and well wrapped with moisturizing materials, and the roots are firmly tied;
Ground-planted soil ball seedlings: the same as bagged seedlings;
One root system must have three or more lateral roots, the lateral root thickness is greater than 0.15mm, the fibrous roots are fresh, and the color is light yellow. 4.4.2 Grade requirements
The quality requirements of each grade of guava grafted seedlings should meet the requirements of Table 1. Table 1 Guava grafted seedling quality grade index
Variety purity/(%)
Height of grafting point/cm
10～30
5Test method
Scion growth length/cm
Seedling stem thickness/cm
5.1 Appearance inspection
Table 1 (continued)
NY/T 689—2003
5.1.1 Visually inspect the degree of pest and disease damage, graft healing, lateral root quantity, and fibrous root color according to quality requirements. 5.1.2 Measure the grafting point height, seedling height, and scion growth length with an ordinary ruler. 5.1.3 Measure the seedling stem and root thickness with a vernier caliper. 5.1.4 Record the grafting point height, tip height, seedling stem thickness, lateral root quantity, lateral root thickness, seedling height, and the judgment level of various seedlings in the table in Appendix A.
5.2 Purity test
Refer to Appendix B, observe the leaf morphology and stem morphology of the sampled seedlings, and determine the number of seedlings of the approved variety. The purity of the variety is calculated according to formula (1):
ml×100
X(%) =
Where:
X——purity,%;
The number of seedlings of the specified variety in the sample, in units of plants; the total number of seedlings sampled, in units of plants.
The calculation result is accurate to one decimal place. The test results shall be recorded in the table in Appendix C. 5.3 Epidemic inspection
It shall be carried out in accordance with the "Plant Quarantine Regulations" of the State Council of the People's Republic of China, the "Implementation Rules of the Plant Quarantine Regulations (Agricultural Part)" of the Ministry of Agriculture of the People's Republic of China and the provisions of GB15569. 6 Inspection rules
6.1 Grouping: The same batch of seedlings is regarded as an inspection batch. 6.2 Sampling: in accordance with the provisions of GB9847.
6.3 Seedling inspection should be carried out when the seedlings are shipped out of the nursery, and the inspection results should be recorded in the table in Appendix C. When the seedlings are shipped out, they must be accompanied by a quality inspection certificate (see Appendix D).
6.4 The judgment rules are as follows:
6.4.1 Grade seedlings: In the same batch of inspected seedlings, 5% of the seedlings are allowed to be lower than the grade one seedling standard, but they should meet the grade two seedling standard. If this range is exceeded, they are grade two seedlings.
6.4.2 Grade two seedlings: In the same batch of inspected seedlings, 5% of the seedlings are allowed to be lower than the grade two seedling standard, and if this range is exceeded, the batch of seedlings is considered unqualified seedlings.
6.4.3 Seedlings that do not meet any of the items in 4.4.1 are not allowed to ship out; seedlings that do not meet the grade two seedling standard are not allowed to ship out. 6.4.4 The three indicators of lateral root quantity, lateral root thickness and fibrous root color are only used for the quality assessment of bare-root seedlings, and are not used for the quality assessment of bagged seedlings and ground-planted soil-ball seedlings.
6.4.5 When seedlings are shipped out, they must have a valid quarantine certificate for the batch and batch of seedlings issued by the inspection or quarantine department. Seedlings without such a certificate are not allowed to be shipped out.
6.4.6 When the trading parties disagree with the inspection results, they must double the sampling and re-inspection times, and the re-inspection results shall be the final results. 7 Packaging and marking
7.1 Packaging
7.1.1 Seedlings in bags
If the seedling bag is not seriously damaged and the soil in the bag is not loose, it is generally not necessary to pack it; if the bag is damaged but the soil is intact, it should be packed in a new bag and then tied with plastic rope, hemp rope, etc.; if the soil is loose, it should be treated as bare-root seedlings. Before packing, the tender branches and leaves and the roots exposed outside the bag should be cut off. 7.1.2 Bare-root seedlings
Water the soil to moisten it one day before seedlings are planted. After seedlings are planted, remove diseased and insect-infested branches and leaves, tender branches and leaves, and most of the old leaves. Keep about one-third of the leaves, and properly trim the overlong lateral roots; put the pruned roots of the seedlings in the mud mixed with rooting agent and moisturizer to make the roots slurry; spread one or more plastic films, straw, sacks, cattail bags, etc. on the ground, fill with moisturizing materials such as coconut bran, and wrap and tie them tightly in bundles of 50 plants.
7.1.3 Ground-planted soil ball seedlings
- 2d to 3d before seedlings are planted, water the soil to moisten it, and use a seedling lifter to lift the seedlings with the trunk of the seedling as the center. The diameter of the soil ball should be greater than 10cm and the height should be greater than 20cm#
-Pack it with plastic bags or films of certain specifications, and then tie it tightly with plastic ropes, hemp ropes, etc. At the same time, cut off some tender branches and leaves, and put the rest of the seedlings in the same bag.
7.2 Marking
Seedlings should be accompanied by seedling labels when they are released from the nursery. Fill in the item column with a non-fading record pen. See Appendix E for labels. 8 Transportation and storage
8.1 Transportation
8.1.1 Seedlings should be shipped as soon as possible according to different varieties and grades, and transported by covered canvas vehicles. 8.1.2 Be careful when loading to prevent the soil ball and bundles from loosening. Pay attention to maintaining a certain humidity and ventilation during transportation. 8.1.3 Transplant or plant as soon as possible after arriving at the destination. 8.2 Compensation for storage
Seedlings should be planted as soon as possible after arriving at the destination. If they cannot be planted within a short period of time, they should be stored as follows. 8.2.1 Place bagged seedlings in a shade shed and pay attention to watering to keep them moist. 8.2.2 Dig a 50cm wide trench in the shade shed and a depth determined by the height of the seedlings, spread fine sand, and plant the bare-root seedlings in the sand in an oblique position, and pay attention to watering to keep them moist.
8.2.3 Dig a shallow trench 30cm wide and 25cm deep in the shade shed or in a shaded place, plant the seedlings in the trench, and pay attention to watering to keep them moist.
Seedling raising unit:
Seedling purchasing unit:
Inspected varieties:
Sample plant number
Height of grafting point/
Reviewer (signature):
Appendix A
(Normative Appendix)
Guava seedling quality inspection record
Number of sampled plants:
Growth length of scion/
Verifier (signature):
Seedling stem diameter/
Number of lateral roots
Tester (signature):
Thickness of lateral roots
NY/T689—2003
Seedling height/
Testing date:
Initial evaluation level
NY/T 689--2003
B.1 Pearl Guava
Appendix B
(Informative Appendix)
Variety Characteristics
The fruit is round to oval, with a longitudinal diameter of 7.2cm~~12.4cm, a transverse diameter of 6.0cm~8.8cm, and a flesh thickness of 1.2cm~2.0cm. When ripe, the fruit is yellow-green in appearance. There are few and soft seeds, and the flesh is crisp and not easy to soften. The mature leaf is 12.7cm~14.7cm long, 6.3cm~7.3cm wide, the petiole is 0.77cm~~1.01cm long, and the internode is 3.3cm~~5.0cm long. The young leaves have brick-red margins, the new shoots are light green, covered with white hairs, the leaf tips are sharp, the margins are entire, and the veins are reticular. The old stems are gray-brown, and the young branches are flat and square. B.2 Seedless guava
The fruit is round to oval, with a longitudinal diameter of 4.9cm~8.2cm, a transverse diameter of 5.0cm~10.7cm, and a flesh thickness of 1.8cm~2.8cm. The appearance is yellow-green when the fruit is ripe. There are few and soft seeds, almost none. The flesh is crisp and not easy to soften. The leaf is 10.5cm~12.7cm long, the leaf width is 5.6cm~6.8cm, the petiole is 0.9cm~1.2cm long, and the internode is 3.8cm~4.8cm long. The new shoots are light green, and the tip is covered with white deaf hairs. The leaf tip is acute, the margin is entire, and the veins are reticular. The young leaves are light green, the leaf margins are brick red, and the petioles, veins and leaf surfaces have a light brick red halo. The tree is open, and the crown is umbrella-shaped under natural growth.
Inspection varieties:
Seedling raising unit:
Total number of plants sampled
Number of seedlings of each grade in the sample
(Normative Appendix)
Guava seedling quality inspection result record sheet No:
Seedling purchasing unit:
The proportion of seedlings of each grade in the sample to the number of seedlings sampled/(%)
Purity of variety/(%)
Inspection conclusion
NY/T689—2003
NY/T689—2003
Seedling raising unit||tt| |Seedling purchasing unit
Seedling varieties
Number of plants planted
Inspection results
Inspection opinions
Inspection unit
(Seal)
Validity period of certificate
Appendix D
(Normative Appendix)
Guava seedling quality inspection certificate
Rootstock varieties
Number of plants sampled
Among them; First level:
Second level:
Year-month-day to Year-month-day
Note: This certificate is in triplicate, one copy each for the seedling raising unit, seedling purchasing unit, and inspection unit. Auditor (signature):
Verifier (signature):
Tester (signature):
Guava seedling label is shown in Figure E.1. bzxZ.net
Appendix E
(Normative Appendix)
Guava seedling label
Guava seedling
Variety name
Nursery unit
Certificate of qualification
Date of nursery
Note: The label is made of 150g kraft paper; the label hole is metal-edged. Figure E.1
a) Front
b) Back
Guava seedling label
NY/T689—2003
Unit is cm77cm~~1.01cm, internode length 3.3cm~~5.0cm. The leaf margin of young leaves is brick red, the new shoots are light green, covered with white hairs, the leaf tips are sharp, the margins are entire, and the veins are reticular. The old stems are gray-brown, and the young branches are flat and square. B.2 Seedless guava
The fruit is round to oval, with a longitudinal diameter of 4.9cm~8.2cm, a transverse diameter of 5.0cm~10.7cm, and a flesh thickness of 1.8cm~2.8cm. The appearance is yellow-green when the fruit is ripe. There are few and soft seeds, almost none. The flesh is crisp and not easy to soften. The leaf length is 10.5cm~12.7cm, the leaf width is 5.6cm~6.8cm, the petiole length is 0.9cm~l.2cm, and the internode length is 3.8cm~4.8cm. The new shoots are light green, and the tip is covered with white hairs. The leaf tips are sharp, the margins are entire, and the veins are reticular. Young leaves are light green with brick-red edges, and petioles, veins and leaf surfaces have light brick-red halos. The tree is in full bloom, and the crown is umbrella-shaped under natural growth conditions.
Inspection varieties:
Seedling cultivation unit:
Total number of plants sampled
Number of seedlings of each level in the sample
(Normative Appendix)
Guava seedling quality inspection result record sheet No:
Seedling purchasing unit:
The proportion of seedlings of each level in the sample to the number of seedlings sampled/(%)
Purity of variety/(%)
Inspection conclusion
NY/T689—2003
NY/T689—2003
Seedling cultivation unit||tt| |Seedling purchasing unit
Seedling varieties
Number of plants planted
Inspection results
Inspection opinions
Inspection unit
(Seal)
Validity period of certificate
Appendix D
(Normative Appendix)
Guava seedling quality inspection certificate
Rootstock varieties
Number of plants sampled
Among them; First level:
Second level:
Year-month-day to Year-month-day
Note: This certificate is in triplicate, one copy each for the seedling raising unit, seedling purchasing unit, and inspection unit. Auditor (signature):
Verifier (signature):
Tester (signature):
Guava seedling label is shown in Figure E.1.
Appendix E
(Normative Appendix)
Guava seedling label
Guava seedling
Variety name
Nursery unit
Certificate of qualification
Date of nursery
Note: The label is made of 150g kraft paper; the label hole is metal-edged. Figure E.1
a) Front
b) Back
Guava seedling label
NY/T689—2003
Unit is cm77cm~~1.01cm, internode length 3.3cm~~5.0cm. The leaf margin of young leaves is brick red, the new shoots are light green, covered with white hairs, the leaf tips are sharp, the margins are entire, and the veins are reticular. The old stems are gray-brown, and the young branches are flat and square. B.2 Seedless guava
The fruit is round to oval, with a longitudinal diameter of 4.9cm~8.2cm, a transverse diameter of 5.0cm~10.7cm, and a flesh thickness of 1.8cm~2.8cm. The appearance is yellow-green when the fruit is ripe. There are few and soft seeds, almost none. The flesh is crisp and not easy to soften. The leaf length is 10.5cm~12.7cm, the leaf width is 5.6cm~6.8cm, the petiole length is 0.9cm~l.2cm, and the internode length is 3.8cm~4.8cm. The new shoots are light green, and the tip is covered with white hairs. The leaf tips are sharp, the margins are entire, and the veins are reticular. Young leaves are light green with brick-red edges, and petioles, veins and leaf surfaces have light brick-red halos. The tree is in full bloom, and the crown is umbrella-shaped under natural growth conditions.
Inspection varieties:
Seedling cultivation unit:
Total number of plants sampled
Number of seedlings of each level in the sample
(Normative Appendix)
Guava seedling quality inspection result record sheet No:
Seedling purchasing unit:
The proportion of seedlings of each level in the sample to the number of seedlings sampled/(%)
Purity of variety/(%)
Inspection conclusion
NY/T689—2003
NY/T689—2003
Seedling cultivation unit||tt| |Seedling purchasing unit
Seedling varieties
Number of plants planted
Inspection results
Inspection opinions
Inspection unit
(Seal)
Validity period of certificate
Appendix D
(Normative Appendix)
Guava seedling quality inspection certificate
Rootstock varieties
Number of plants sampled
Among them; First level:
Second level:
Year-month-day to Year-month-day
Note: This certificate is in triplicate, one copy each for the seedling raising unit, seedling purchasing unit, and inspection unit. Auditor (signature):
Verifier (signature):
Tester (signature):
Guava seedling label is shown in Figure E.1.
Appendix E
(Normative Appendix)
Guava seedling label
Guava seedling
Variety name
Nursery unit
Certificate of qualification
Date of nursery
Note: The label is made of 150g kraft paper; the label hole is metal-edged. Figure E.1
a) Front
b) Back
Guava seedling label
NY/T689—2003
Unit is cm
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