This standard specifies the methods and basic requirements for field efficacy plot tests of herbicides for controlling weeds in non-arable land. This standard applies to field efficacy plot tests and efficacy evaluation for registration of herbicides for controlling weeds in non-arable land, including industrial areas, warehouses, railways, sidewalks, and other plots where weeds are not desired to grow, with the purpose of controlling all weeds (but not including road boundaries, parks, and sports fields). Other field efficacy tests shall be carried out in accordance with this standard. GB/T 17980.51-2000 Guidelines for Field Efficacy Tests of Pesticides (I) Herbicides for Controlling Weeds in Non-arable Land GB/T17980.51-2000 Standard download decompression password: www.bzxz.net
This standard specifies the methods and basic requirements for field efficacy plot tests of herbicides for controlling weeds in non-arable land. This standard applies to the field efficacy plot test and efficacy evaluation of herbicides for registration to control weeds in non-arable land, including industrial areas, warehouses, railways, sidewalks, and other plots where weeds are not desired to grow, with the purpose of controlling all weeds (but not including road boundaries, parks, and sports fields). Other field efficacy tests shall be carried out in accordance with this standard.

GB/T17980.51--2000
Field efficacy test is one of the important contents of pesticide registration management in my country, and is an important technical basis for the formulation of pesticide product labels. The label is the only guide for farmers to use pesticides safely and reasonably. In order to standardize the methods and contents of field efficacy tests for registration, make the tests more scientific and unified, and align with international standards, so that my country's efficacy test reports have international recognition, the national standards for field efficacy test guidelines in my country are specially formulated. This series of standards refers to the field efficacy test guidelines of the European and Mediterranean Plant Protection Organization (EPPO) and similar guidelines in the Asia-Pacific region of the Food and Agriculture Organization of the United Nations (FAO). It is formulated based on my country's actual situation and verified by a large number of field tests. In production, many weeds in non-arable land often need to be controlled with herbicides. In order to determine the optimal field dosage for controlling weeds in non-arable land, test the effects of pesticides on non-target beneficial organisms, and provide a basis for the efficacy evaluation and safe and reasonable use technology of non-arable land herbicide registration, this standard is specially formulated.
This standard is one of the standards in the series of standards for the field efficacy test of pesticides (I), but it is an independent standard. This standard is proposed and managed by the Ministry of Agriculture of the People's Republic of China. This standard was drafted and interpreted by the Pesticide Testing Institute of the Ministry of Agriculture. The main drafters of this standard are Ye Guibiao, Wei Fuxiang, Jia Fuqin, Liu Xue, and Wang Huanmin. 566
1 Scope
National Standard of the People's Republic of China
Guidelines for the field efficacy trials (I) Herbicides against weeds in no-crop field GB/T 17980.51-2000
This standard specifies the methods and basic requirements for field efficacy trials of herbicides against weeds in no-crop field. This standard is applicable to field efficacy trials and efficacy evaluation of herbicides for registration in controlling weeds in non-crop land, including industrial areas, warehouses, railways, sidewalks, and other plots where weeds are not desired to grow, with the purpose of controlling all weeds (but not including road boundaries, parks, and sports fields). Other field efficacy trials shall be carried out in accordance with this standard. 2 Experimental conditions
2.1 Selection of crops and cultivars
2.2 Selection of test weeds
The non-arable land for the test must have representative weed populations and be evenly distributed. The weed community composition must be consistent with the herbicide spectrum of the test (grasses, sedges, broadleaf weeds, annuals, perennials). Record the Chinese names and Latin names of various weeds. 2.3 Environmental conditions
All conditions in all test plots must be uniform. Record any type of herbicides used before and after. 3 Experimental design and arrangement
3.1 Agents
3.1.1 Test agents
Indicate the trade name/code, Chinese name, generic name, dosage form content and manufacturer of the test agent. The test agent shall be treated with no less than three doses, or the amount of drug specified in the agreement (the test agreement signed by the test entrusting party and the test undertaking party). 3.1.2 Control Agents
Control agents must be registered products with good safety and efficacy in practice. The type and mode of action of control agents should be similar to those of test agents and the dosage and treatment methods commonly used in the local area should be used. Special circumstances may depend on the purpose of the test. Set up a blank control treatment. 3.2 Plot Arrangement
3.2.1 Plot Arrangement
The plots for different treatments in the test are arranged in random blocks. In order to solve the problem of uneven weed distribution, the test plots for controlling perennial weeds may be arranged irregularly and explained. 3.2.2 Plot Area and Repetition
GB/T 17980. 512000
Net plot area: 30-50m2. When spraying with a sprayer carried on rails on the railway, the plot shall be at least 100m2. Repeat times: at least 4 times.
3.3 Application method
3.3.1 Usage method
Perform according to the requirements of the agreement and the instructions on the label. Commonly used spraying methods or withdrawal of granules, etc. 3.3.2 Use of equipment
Select the equipment commonly used in production, and use a sprayer with a stable pressure and a fan-shaped nozzle for spraying to ensure that the pesticide is evenly distributed throughout the plot, or that the liquid medicine falls accurately and directional to the place where it should be treated. Record all information such as the type of equipment used and operating conditions (operating pressure, nozzle diameter, nozzle type, soil mixing depth). The amount of pesticide should be accurate during application. If the deviation of the amount exceeds ±10%, it should be recorded, and the factors affecting the efficacy and the duration and selectivity of weed control should be recorded. 3.3.3 Application time and frequency
Perform according to the requirements of the agreement and the instructions on the label. The time of application must be consistent with the development characteristics of weeds. a) Spring treatment;
b) Early summer treatment;
c) Summer treatment.
Record the growth status of weeds (germination, growth stage) when applying the pesticide. If the label or protocol does not specify the number of times of application, it should be determined according to the purpose of the test and the characteristics of the active ingredients of the product. The same product can be used once or in batches. Record the number and time of application, as well as the growth status of the weeds (germination, growth period). 3.3.4 Dosage and water consumption
Apply the pesticide according to the label instructions or the dosage and water consumption required by the protocol. Usually the dosage of the pesticide is expressed in g/hm2 (grams/hm2) of the active ingredient, and the water consumption is expressed in L/hm2 (liter/hm2). If the water consumption is not specified in the protocol, the water consumption can be determined based on the mode of action of the test pesticide, the type of sprayer, and local experience. 3.3.5No information requirements for pesticides used to control pests and non-target weeds.
4 Investigation, recording and measurement methods
4.1 Meteorological and soil data
4.1.1 Meteorological data
During the test, data such as rainfall (rainfall type and rainfall in mm), temperature (daily average temperature, maximum and minimum temperature in °C), wind speed, weather, light and relative humidity should be obtained from the test site or the nearest meteorological station, especially the meteorological data on the day of application and the 10 days before and after.
The adverse climatic factors that affect the test results during the entire test period, such as severe or long-term drought, heavy rain, ice, etc., must be recorded. 4.1.2 Soil data
Record soil type (including composition), soil pH value, organic matter content, etc. 4.2 Investigation methods, time and frequency
4.2.1 Weed investigation
Detailed description of the symptoms of weed damage (such as growth inhibition, chlorosis, dead spots, deformity, etc.) to accurately explain the mode of action of the pesticide. Record the weed population in the plot, such as weed species, number of weeds, coverage or weed weight, etc., using the absolute value method or estimated value method. 4.2.1.1 Absolute value survey method
Survey the total number or weight of each weed, survey the entire plot or randomly select 3 to 4 points in each plot, and conduct a sampling survey of 0.25~1m2 at each point. In some cases, investigate the organs of the weeds (such as grass score), etc. 4.2.1.2 Estimated value survey method
GB/T 17980. 51--2000
Each pesticide treatment area is compared with the adjacent blank control area or control strip to estimate the relative weed population. This survey method includes the overall weed community and single weeds, and can use indicators such as weed number, coverage, height and growth (such as actual weed quantity). The estimation method is fast and simple, and the result can be expressed as a simple percentage (0 means no weeds, 100% means the same weeds as the blank control area), or converted to express the percentage effect of weed control (0 means no control effect, 100% means all weeds are controlled). The absolute value of the weed population coverage in the blank control area or control strip should also be recorded. In order to overcome the difficulties of accurately estimating percentages and using homogeneous variance, the following grading standards can be used for investigation:
Grade 1: no grass;
Grade 2: equivalent to 0~～2.5% of the blank control area; Grade 3: equivalent to 2.6%~～5% of the blank control area; Grade 4: equivalent to 5.1%~10% of the blank control area; Grade 5: equivalent to 10.1%~15% of the blank control area; Grade 6: equivalent to 15.1%~25% of the blank control area; Grade 7: equivalent to 25.1%~35% of the blank control area; Grade 8: equivalent to 35.1%~~67.5% of the blank control area; Grade 9: equivalent to 67.6%~100% of the blank control area. Investigators must be trained before using this grading standard. This grading range can be used directly without converting it into the average value of the estimated value percentage.
4.2.2 Survey time and frequency
a) Spring and early summer treatment
First survey: 2 to 4 weeks after treatment.
Second survey: 5 to 6 weeks after treatment.
Third survey: in autumn, before weeds in the control area begin to senesce. Fourth survey: in the second year, after weeds in the control area re-sprout. b) Summer treatment
First survey: 2 to 4 weeks after treatment.
First survey: 5 to 6 weeks after treatment, before weeds in the control area begin to senesce. Third survey: in the second year, after weeds in the control area re-sprout. Intermediate surveys may be conducted if necessary.
4.2.3 Calculation method of drug efficacy
The drug efficacy is calculated according to formula (1):
Control effect (%)=
Where: PT----Number of remaining grass (or fresh weight) in the drug treatment area; (K-Number of live grass (or fresh weight) in the blank control area. 4.3 Crop survey
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None. However, attention should be paid to the possibility of the herbicide being transmitted to adjacent trees or plants through root contact. 4.4 Side Effect Observation
Record the effects on non-target organisms.
5 Results
Use the Dunbeck's Multiple Range (DMRT) method to statistically analyze the test data, and use corresponding biostatistical methods in special cases. Write a formal test report, analyze and explain the results, and put forward conclusive opinions on the application effect evaluation (product characteristics, key application technology, applicable period and dosage, herbicide spectrum, efficacy, and drug damage) and economic benefit evaluation. The test report should list the original data. 569
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