Some standard content:
ICS 65.020.01
Agricultural Industry Standard of the People's Republic of China
NY/T1489—2007
Rules for the trial technology of potato
Potato
Rules for the trial technology of potato varieties2007-12-18 release
2008-03-01 implementation
Ministry of Agriculture of the People's Republic of China
Appendix A of this standard is a normative appendix. Attachment (is an informative appendix. This standard is proposed and managed by the Ministry of Agriculture of the People's Republic of China. NY/T1489--2007
The main drafting units of this standard are: National Agricultural Technology Extension Service Center, Vegetable and Flower Research Institute of Chinese Academy of Agricultural Sciences, Crop Research Institute of Qinghai Academy of Agricultural and Forestry Sciences, Agricultural College of Northeast Agricultural University. College of Crop Sciences of Fujian Agriculture and Forestry University, Beijing Seed Management Station, Heilongjiang Seed Management Bureau, Hubei Enshi Southern Maple Research Center. The main drafters of the standard are: Liao Qin, Zou Kui, Xie Kaiyun, Zhang Yongcheng, Shi Ying, Shuai Zhaoshi, Zhao Qingchun, Zhang Sitao, Huang Daen, Sun Linhua, Shi Xiaohong, Wang Chunling, Xian Hong, Zhai Yingfen, Cao Debin. 1 Scope
, Potato||tt| |Technical Regulations for Crop Variety Tests
This standard specifies the technical requirements and methods for regional tests and production tests of potato varieties: This standard is applicable to the national and provincial crop variety committees to carry out potato variety tests. 2 Normative References
NY/T1489—2007
The clauses in the following documents become the clauses of this standard through reference in this standard: For any referenced document with a date, all subsequent amendments (excluding errata) or revisions are not applicable to this standard. However, the parties to an agreement based on this standard are encouraged to study whether the latest versions of these documents can be used. For any referenced document without a date, the latest version shall apply to this standard. GB7331 Inspection Procedure for Origin of Potato Seed Potatoes (318133 Detoxified Black Potato Seed Potatoes
3 Terms and Definitions
The following terms and definitions apply to this standard:
Growth period grinvth perind
The number of days from the emergence stage to the maturity stage.
Dry matter
Matter other than water in a tuber. 3.3
Starch content
The percentage of starch mass in a tuber to the mass of fresh tubers. 3.4
Fried color
The color of the fresh tubers after frying under certain conditions. 3.5
Tuber shape
The shape of a tuber after normal development and maturity.
Tuber defect
Defects inside and outside the tuber, such as deformity, cracking, hollow black circle, black heart, bad snake sugar end, green skin, worm eyes, etc. 3.7
Marketability
The percentage of tuber mass that meets the requirements of commercial tuber to the total mass of harvested tubers 4 Test varieties
4.1 Regional test varieties
NY/T 1489—2007
--Selected or discovered by individuals and improved;---Obviously different from existing varieties;
--Relatively stable genetic traits;
--Morphological characteristics and biological characteristics are consistent;--Genetically modified varieties should provide agricultural genetically modified biosafety certificates;---Have appropriate variety names.
4.2 Production test varieties
Varieties with good overall performance in regional tests. 5 Test units
5.1 Field test units
Based on natural conditions (such as climate, topography and soil, etc.) and cultivation conditions, a certain number of representative units in each ecological zone will be selected to undertake field test tasks.
5.2 Resistance identification and quality inspection unit
Resistance identification and quality inspection unit determined by the Crop Variety Approval Committee. 6 Experimental setting
6.1 Experimental site selection
Select a plot with flat terrain, consistent previous crop, medium fertility, convenient drainage and irrigation, and representative plots as experimental sites, which shall not be affected by shade from buildings, forests, forest belts, etc. The previous crop cannot be a sticky crop, and other Solanaceae crops cannot be planted around the experimental site: 6.2 Seed potato requirements
Seed potatoes should be strictly selected, with consistent sources and standards, and meet the requirements of "Multi-de-longevity Potato Seed Potatoes" (CB1S133) and "Potato Seed Potato Field Inspection Procedures CB7531). It is advisable to sow whole potatoes. If sowing in pieces, each variety should be cut into pieces, and each piece should have more than 2 buds. When cutting, eliminate diseased and rotten potatoes, and the cutting knife should be disinfected and replaced; the second cropping area should be sown in whole potatoes. The breeding units of production test varieties and the seed supply units of control varieties shall provide first-class seeds to each production test undertaking unit one month before sowing. 6.3 Seed propagation
Each breeding unit shall mail (deliver) virus-free micro-tubes to the seed propagation unit as required, and after uniformly producing qualified seeds under isolation conditions such as temperature and net, distribute them to each undertaking unit.
6.4 Experimental design
6.4.1 Regional test The regional test of each variety shall be no less than 5 test points in the same ecological type area, with a plot area of 13.33m~20m2. The test time shall be no less than two production cycles: each group of test varieties shall be no less than 4, and when there are 4 varieties. Latin square design: when there are more than 4 varieties, a random complete block design shall be repeated 3 times. The white row design of each test point shall be arranged by interval: no walkway shall be left between the plots in the block group: no less than 2 rows of protection rows shall be planted on the edge of the test area. 6.4.2 Production test: The production test of each variety shall be conducted at no less than 5 test sites in the same ecological type area. The planting area of one test site shall be no less than 300m2 and no more than 3000m2. The test time shall be one production cycle. Each test point shall be designed with an interval arrangement diagram. The number of rows planted around the test area shall be no less than 2 rows and 7 rows shall be expanded. Cultivation and management
7.1 Seed potato treatment before sowing
To ensure the emergence rate after sowing, seed potato germination treatment shall be carried out before sowing: 7.2 Sowing period
Sowing shall be carried out according to the local suitable sowing period:
7.3 Planting density
7.3.1 Regional test
NY/T 1489—2007
The plot area of the winter crop area is 20m2, 1 row area, and 120 plants are sown; the plot area of the early-maturing cotton and two-crop area is 20m2, 5 row area, and 120 plants are sown; the plot area of the mid- and late-maturing North China, Northeast China, and Northwest China areas is 20m2, 5 row area, and 100 plants are sown; the plot area of the late-maturing Southwest area is 13.33m2, row area, and 80 plants are sown.
7.3.2 Production test
Carry out according to local planting density.
7.4 Field management
According to local climate and cultivation conditions, choose the right day and sow at the right time. After emergence, timely inter-cultivation and weeding, keep the soil loose, timely soiling, irrigation, fertilization, and prevention and control of diseases and insect pests. The management measures for planting and cultivation must be consistent, and the same management measures must be completed on the same day. If there are difficulties, at least one in each area group must be guaranteed.
7.5 Harvest
When harvesting, count the number of harvested plants first. If a regional test lacks more than 15% of plants in a plot, it should be treated as a missing area; if there are 3 plots in a test that lack more than 15% of plants, the test will be scrapped. The test is calculated based on the yield of the entire plot. 8 Investigation and Recording
Investigation and recording shall be conducted according to the "Investigation and Recording Items and Basis of Potato Variety Trial", see Appendix A. The investigation and recording results of the day shall be recorded in the "Investigation and Recording Form for Potato Variety Trial", see Appendix B, and the results shall be sorted out and filled in the "Annual Report of Potato Variety Trial", see Appendix (. 9 Disease Resistance Assessment
9. 1 Potato Virus Disease X and Virus Disease Y Resistance Identification The breeding units of the participating varieties of the production test shall send (deliver) 200 original seeds with a grain weight of 5 or more that have passed the dormant period to the designated units of the Variety Approval Committee for disease resistance assessment before the end of January. 9.2 Late Blight Resistance Identification
The breeding units of the participating varieties of the production test shall send (deliver) 200 original seeds with a grain weight of 5 or more that have passed the dormant period to the designated units of the Variety Approval Committee for disease resistance assessment during the period from March 1 to 15. The above 100 original seeds shall be mailed (delivered) to the designated unit of the Variety Approval Committee for disease resistance identification. 10 Quality Inspection
Within 10 days after the tubers are harvested, each unit undertaking the production test shall take 2.5 kg of samples of each variety from the fully mixed samples and send (deliver) them to the designated unit of the Variety Approval Committee for tuber quality inspection. 11 Test Summary
11.1 Sending of Investigation and Record Results
Each test unit undertaking the resistance identification unit and the quality inspection unit shall send (or send by email) two copies of the "Potato Variety Test Annual Report", the disease resistance identification report, and the quick-stem quality inspection report to the test management department and the headquarters within 1 month after the test.
11.2 Test report
If the unit responsible for the test has any of the following circumstances, the test shall be scrapped: a) The test is terminated unexpectedly due to force majeure; b) The number of missing plants in the three plots exceeds 15%; c) The error coefficient of variation exceeds 15%; d) The average total yield is lower than 50% of the average total yield of all test points in the group; e) The "Annual Test Report of Potato Varieties" is not sent or emailed on time; [) Other serious violations of the technical regulations for potato variety tests and serious impact on the scientific nature of the test. If the test is scrapped due to force majeure, the unit responsible shall notify the main department and the summary unit of the test within half a month. 11.3 Data processing
11.3.1 Investigation and record contents
Regional trials should investigate and record all items in the "Potato Variety Trial Investigation Record Form"; production trials should investigate and record the phenological period, plant morphological characteristics, field characteristics, tuber characteristics, major diseases and physiological defects in the "Potato Variety Trial Investigation Record Form"; the breeding results should investigate and record the phenological period, plant morphological characteristics, tuber characteristics, physiological defects, disease occurrence, breeding quantity (number of plots), etc. in the "Potato Variety Trial Investigation Record Form", and make a simple comment on the variety in terms of growth period, purity, characteristics, etc., and put forward the basis for varieties with serious defects in certain aspects to be unsuitable for participating in regional trials. 11.3.2 Data analysis
Each responsible unit and summary unit shall conduct statistical analysis and comprehensive evaluation of the test data, and each test shall use the plot tuber products for variance analysis and home weight comparison.
12 Others
The test seeds received by each undertaking unit can only be used for variety testing. After ensuring the smooth implementation of the test, the excess seed potatoes and the breeding materials produced by the test varieties shall be destroyed in time and cannot be used for breeding, reproduction, exchange and other activities: It is strictly forbidden to receive the breeding (introduction) units and relevant enterprises to inspect and understand the test varieties. Violators will be disqualified from the test; if any abnormal behavior of the relevant units is found, the situation should be reported to the National Agricultural Technology Extension Service Center, the competent department of the regional test, in a timely manner. If there is any violation, the person will be held accountable according to law. 4
A.1 Field design
Appendix A
(Normative Appendix)
Material variety test survey record items and basis number of test varieties, control varieties, plot arrangement, number of repeated drinking, planting density, plot area, etc. A.2 Meteorological and geographical data
A.2.1 Temperature: the average monthly maximum, minimum and average temperature during the growth period. A.2.2 Precipitation: number of days with precipitation, precipitation and distribution during the growing period. A.2.3 First frost time, last frost time.
A.2.4 Latitude, longitude, altitude.
A.3 Basic situation of the experimental site and cultivation management
A.3.1 Basic situation
Preliminary conditions, soil type, tillage and land preparation methods, etc. A.3.2 Cultivation management
NY/T 1489 2007
Sowing methods and methods, fertilization, intertillage and weeding, drainage, pest and disease control, etc. At the same time, record special events that occur during the growing period.
A.4 Phenological period
Randomly survey 2 plots and take the average value of 2 times. A.4.1 Sowing period: the date of the day of sowing
A.4.2 Seedling period: the date when the emergence rate of the plot reaches 50%: survey every other day after the emergence of seedlings. A4.3 Storage period: the date when 50% of the plants have buds. Fragrance adjustment every other day after the beginning of buds. A.4.4 Flowering period: the date when 50% of the plants have flowers. Survey every other day after the beginning of flowering. A4.5 Maturity period: the date when 50% of the leaves in the plot turn yellow. Survey twice a week in the late growth period. A.4.6 Harvest period: the date of tuber harvest. A.4.7 Growing period: the number of days from the Hi yellowing period to the maturity period. A.5 Plant morphological characteristics
Randomly survey 2 plots.
A.5.1 Purple color: green, lavender, reddish brown, purple, green with brown, purple net, pot color with green net pattern, etc. A.5.2 Leaf color: light green, green and dark green. A.5.3 Flowering profusion: recorded from the bud stage to the full flowering stage, divided into: no buds, bud drop, few flowers, medium and profuse, A.5.4 Corolla color: observed the newly opened flowers before 10:00 am during the full flowering stage. Divided into: white, light red, dark red, light gray, dark blue, light purple, dark purple and yellow.
NY/T1489-2007
A.5.5 Fruiting: divided into: especially, few, medium and many. 4.5.6 Creep length: divided into: short, medium and long, A. 6 Field traits
Seedling rate is investigated by plot, and other traits are randomly investigated in 3 plots, with 10 plants in each plot, a total of 30 plants, and the average value is taken. 4.6.1 Seedling rate: the percentage of plants that emerge in a plot to the number of sowing holes, investigated during the bud stage. A.6.2 Number of tubers: the number of tubers growing directly from the seedling or underground, adjust the ridge during the flowering period. A.6.3 Plant height: the height from the soil surface to the top of the tuber, adjust the feeding during the flowering period. 4.6.4 Number of tubers per plant: the number of tubers per plant, investigate at harvest. A.6.5 Tuber quality per plant; investigate at harvest, A.6.6 Tuber quality per tuber: calculate by dividing the mass of a single tuber by the number of tubers per plant. 4.7 Tuber traits
A.7.1 Investigation of tuber quality traits
Randomly investigate 2 plots at harvest.
1.7.1.1 Uniformity of tuber size: irregular, medium and uniform. A.7.1.2 Tuber shape: round, oblate, oblong, oval, oblong, elliptical and oblong, etc. A.7.1.3 Skin color: milky white, light yellow, yellow, ivory, pink, red, purple, purple, dark purple and others. A.7.1.4 Flesh color: white, milky white, light yellow, yellow-red, charcoal purple, purple and others. A.7.1.5 Skin type: smooth, slightly hemp skin, hemp skin and heavy hemp skin and others. A.7.1.6 Tuber eye depth: the relative depth between the eye and the epidermis, divided into protruding, shallow, medium and deep, depth <.1m is shallow, 1m~3mm is medium, >3 mm is deep.
A.7.2 Tuber quantitative traits survey
All plots are surveyed at harvest.
A.7.2.1 Commercial potato rate: Tubers are graded by size and weighed at harvest to calculate the commercial potato rate. Fresh edible varieties: Southwest, two-season cropping area, winter cropping area single potato weight 50g (inclusive) or more, single potato weight 75g (inclusive) or more in the one-season cropping area is a commodity; French fries processing varieties single potato weight 150g (inclusive) or more is a commodity potato, potato chips processing varieties single potato diameter 1 cm ~ -10 cmn is a commodity potato.
A.7.2.2 Specific gravity: Determine with water specific gravity method within 1 week after harvest. According to the proportion of large, medium and small pieces of each variety, take 5kg of mixed samples from each repetition. Weigh the mass of the block in the air - m2 and the mass of the block in the water m2+ respectively. Calculate according to formula (A.1): Specific gravity = m2/(m2-m2) --
Block solid matter content: According to the specific gravity, check the Mepkcp dry matter content table 4.7.2. 3
Mcpkep dry matter content table
5kg tuber water
medium weight (g)
dry matter
content (3)
starch content
5 tuber water
weight (g))
ten substances
content ()
spinning bone mass
5kg block steamed water||tt ||Medium weight (g)
Tuber physiological defects
"Matter
Content (5)
16-214
20,670
Table (continued)
Starch content
13,541
13-898
5kg tuber water
Weight (g)
NY/T 1489—2007
Dry matter
Content (%)
Starch content
Secondary growth: 10 plants were randomly surveyed in each plot at harvest, a total of 30 plants were surveyed, and the percentage of secondary growth tubers was calculated. A.8.2
Cracked tubers: 10 plants were randomly surveyed in each plot at harvest, a total of 30 plants were surveyed, and the percentage of cracked tubers was calculated. 4.8.3 Hollow tubers: 10 plants were randomly surveyed in each plot at harvest, a total of 30 plants were surveyed, and the percentage of hollow tubers was calculated. Percentage of heart blocks. A.9 Main diseases
Randomly survey 10 plants in each plot. And survey 30 plants. A.9.1 Potato mosaic virus disease: Survey in the late flowering period to calculate the incidence rate and disease index, incidence rate (%) -
Number of diseased detections
Total number of investigations
Disease index = "(Number of diseased plants × representative value) / (Total number of investigations × highest representative value) × 100) A, 9.2 Potato leaf roll virus disease: Same as mosaic virus disease. A.9.3. Rot: Investigate during the young stage and ring-flowering stage of the plant; randomly select 30 tubers after harvest and cut open the navel of the tuber for investigation, and calculate the incidence rate and disease index.
A.9.4. Blight: Record the earliest diseased plant date in the plot, investigate the diseased plants every two weeks after the first onset, and finally calculate the white percentage of diseased plants during the entire growth process.
A.9.5. Late blight: The earliest date of diseased spots in the plot is the onset period. Investigate the incidence rate and disease index every week after the first onset, and divide them into 0, 1, 2, 3.1 levels.
A.9.6. Early blight is the same as late blight.
NY/T1489-2007
4.10. Virus diseases and disease classification standards
A.10.1. Mosaic virus disease
Grade 0, no symptoms.
1: The plant size is similar to that of a healthy plant, the leaves are flat but the young leaves have more or less yellow-green spots of varying sizes. Level 2: The plant size is similar to that of a healthy plant or slightly shorter, the leaves of the main part have clear mosaic or slight wrinkles, and sometimes have necrotic spots. Level 3: The plant is dwarfed, the branches of the whole plant are reduced, most leaves are severely mosaic, wrinkled or deformed, and sometimes have necrotic spots. Level 4: The plant is obviously dwarfed, with fewer branches, and the leaves of the whole plant are severely mosaic, wrinkled or distorted. Some leaves are necrotic, the lower leaves fall off, and even the plant dies early.
A.10.2 Leaf Roll Virus Disease
Level: No symptoms.
Grade 1: The plant size is similar to that of the healthy plant, the top leaves are slightly bundled. Green or only the lower compound leaves start from the top leaflet, and roll up along the edge into a spoon shape, which is brittle and easy to break.
Grade 2: The diseased plant is slightly lower than the healthy plant, half of the leaves are spoon-shaped, the lower leaves are severely rolled into a tube, and the pith is easy to break. Grade 3: The diseased plant is short, most of the leaves are rolled into a tube, the middle leaves are severely rolled into a tube, and sometimes a few leaves are completely withered: Grade 1: The diseased plant is extremely short, the leaves of the whole plant are severely rolled into a tube, and some or all of them fall off. A.10.3 Ring rot
A.10.3.1 Plant symptoms
Grade C: No symptoms.
Grade 1: A few leaves of the plant are wilted.
Grade 2: Most or part of the plant branches are climbing bananas, yellow flowers between the veins, and the leaf edges are scorched. Grade 3: The whole plant wilts, yellow flowers, and dies:
A.10.3.2 Tuber symptoms
Grade: No symptoms.
Grade 1: There is obvious mild disease, and the diseased part is less than 1/4 of the microtubule bundle ring. Grade 2: The diseased part occupies 1/1~3/4 of the microtubule bundle ring. Grade 3: The diseased part occupies more than 3/4 of the microtubule bundle ring. A.10.4 Late blight
Grade 0: No symptoms,
Grade 1: There are individual lesions on the leaves,
Grade 2: 1/3 of the leaves have lesions.
3 Velvet: 1/3~-1/2 of the leaves have lesions. Level 4: 1/2 leaves are susceptible.
A.11 Harvest data
A.11.1 Harvest area: the harvest area of each plot. A.11.2 Number of harvested plants: the number of harvested plants in each plot. 4.11.3 Plot yield: weighed at harvest.
Appendix R
(Normative Appendix)
Potato variety trial investigation record form
Trial executor:
Address:
NY/T 1489
NY/T1489—2007
B.1 Field design
Basic information
Test varieties
Row area. Plot area
B.1.2 Field arrangement diagrambzxz.net
B.2 Meteorological and geographical data during the test period
Meteorological data
Average maximum temperature (℃)
Average minimum temperature (%)
Average temperature <
Number of water dogs d)
Precipitation (mm)
Yang Li time
Frost time
B.2.2 Geographical data
Non-column, repeated
m\, plant spacing
, longitude
B.3 Basic information of the test site and cultivation management
B.3. 1 Basic situation
Previous crop:
Soil type:
B.3.2 Cultivation and management
B.3.2.1 Sowing
Sowing period:
B.3.2.2 Intertillage and weeding
Time and frequency:
B.3.2.3 Fertilization
.Harvest period:
day, sowing method and method:
Base fertilizer (time, type, method);
Forced fertilizer (time, type, method):
B.3.2.4 Irrigation and drainage conditions
Irrigation (time, number of times):
times, row length
m, density every 666.7m
Altitude
Cultivated land and land preparation method;
m, row spacing
plants, total test net area1 Basic situation
Previous crop:
Soil type:
B.3.2 Cultivation and management
B.3.2.1 Sowing
Sowing period:
B.3.2.2 Intertillage and weeding
Time and frequency:
B.3.2.3 Fertilization
.Harvest period:
day, sowing method and method:
Base fertilizer (time, type, method);
Forced fertilizer (time, type, method):
B.3.2.4 Irrigation and drainage conditions
Irrigation (time, number of times):
times, row length
m, density every 666.7m
Altitude
Cultivated land and land preparation method;
m, row spacing
plants, total test net area1 Basic situation
Previous crop:
Soil type:
B.3.2 Cultivation and management
B.3.2.1 Sowing
Sowing period:
B.3.2.2 Intertillage and weeding
Time and frequency:
B.3.2.3 Fertilization
.Harvest period:
day, sowing method and method:
Base fertilizer (time, type, method);
Forced fertilizer (time, type, method):
B.3.2.4 Irrigation and drainage conditions
Irrigation (time, number of times):
times, row length
m, density every 666.7m
Altitude
Cultivated land and land preparation method;
m, row spacing
plants, total test net area
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