GB/T 2930.4-2001 Inspection procedures for forage seeds Germination test
Some standard content:
Ics 65.020-20
National Standard of the People's Republic of China
GB/T 2930.1~-2930.11—2001
Rules for inspection of forage seeds
Rulcs for forage seeds 200103·14 issued
2001-06-01 implementation
National Quality and Technical Supervision Commission issued
GB/T 7930.4—2001
This standard is based on the International Plant Testing Association (1569 edition) G32.93)—1932 Pastoral Plant Testing Procedure:
This standard is based on the International Plant Testing Association (1569 edition) G32.93)—1932 Pastoral Plant Testing Procedure:
This standard has the following main contents: biological and testing methods, etc., and has reduced the international standard (151A13) germination test. In addition, the document has been limited to six people, but in my country there are some suitable testing methods for economic value. The standard not only has the scientific advantages of international standards, but also meets the practical needs of my country's species verification. The main changes of this standard (12031-1982) are: the value of species has been increased, and the number of species has increased by 24 from the previous 115. In addition, the definition, numerical value, indication, reference and other aspects have been supplemented and revised. This standard product (H293.1290.1-2013) is one of the 3 series of standards for inspection procedures for livestock species. The following GR/T 20S(.121:-
GHT2O0.2—2GG
Grass seed harvesting process
Grass seed quality standard
GB/TE30.231
This is also used as a sub-screening standard
GB/T 080. 4—2U31
GhT 290-20]
GB:T 2A30. 6--2C:
GB/T 2950.72CC-
GB/T 9U30, K—20.
Modified one inspection procedure
I'm in the new year! , Inspection procedure
Clarity analysis
Comparison of the number of particles in any material
Drug inspection
Production of biological (tetrazolyl) determination scientific procedures · Can determine the species and varieties to be produced
Receiving a single variety of inspection procedures
Agricultural leather varieties for inspection procedures
Moisture content measurement
【H29302 Animal Husbandry Inspection Procedures
GB/T292:0 .1G—2531 Test for coated blue seeds
G/120.231 This test heat flow test report
The revised standard is different from the current international definition in terms of overall and format. The original national standard hopes to put all the annexes after the main text of the test items. However, most of the industrial version is in the annexes, which is inconvenient to refer to each other when it is missing. Since the compilation of this standard, each item has been compiled as a separate standard, the rich content is presented in the small text, and the relevant references are summarized in the text of each tour! This standard is based on GB2-1U82: A, B, and the performance of this standard are all standard appendices. The standard is proposed by the Ministry of Agriculture and the Ministry of Agriculture. The originating unit of this standard is the Ministry of Agriculture and the Ministry of Agriculture. The main author of this standard is the Ministry of Agriculture and the Ministry of Agriculture. The main author of this standard is the Ministry of Agriculture and the Ministry of Agriculture. The drafters are Sun Jihua, Shang Zhibiao, Xue Quanjie, Zeng Yanjun. GB/1 2930.4—2001 ISTA Attached piece
One of the biggest risks in the agricultural industry is that the varieties cannot show their production capacity: the purpose of seed inspection is to assess the quality of the varieties before planting, so as to reduce this risk to the minimum. The concept of seed pressure is a combination of internal and external factors: these characteristics are good for the industry producers, processors, operators, management, certification bodies and government agencies or departments responsible for seed management. In all cases, the scope of inspection is to determine the value of the species: the species is a living product, and the basic value of the biological product can only be predicted through the inspection. The method used must be based on scientific knowledge and the experience of the test author. The required derivation and performance depends on the test period. This regulation defines the standards and methods for the assessment of seed quality in international trade. To this end, the difficulty and reproducibility of the inspection methods must be reduced. When the seeds are traded beyond the boundary, there is a possibility that they will be inspected in different laboratories in different countries. Therefore, all inspections should be conducted using the same standard method. This is to achieve consistent inspection results within the production range. This regulation is divided into two parts: the procedure and the appendix: the procedure document part explains the principles of the determination of the items, the definitions adopted, and an overview of the methods and procedures used. The technical part summarizes the definitions and details the procedures and methods specified in the procedure. The results of the inspection and registration of the international seed association's certificate must be strictly followed. The interpretation of the item record in the procedure should be consistent with the relevant section in the appendix to this chapter. When the national seed quality management regulations are not implemented within the scope of the national seed management regulations, the regulations and their related documents should be used as much as possible. Although this does not necessarily require the use of international seed inspection certificates, we recognise that non-compliance with this internationally defined standard and its accompanying provisions could hinder the movement of seeds between countries. Advisory tests are tests that require specific requirements and are carried out for the purpose of evaluating seed resistance. These tests take into account the crop, type of seed and sea level, etc. For each seed case, this standard and the equipment provided by this standard provide guidance. Other technical references may be used as supplementary information. This standard and its annexes are developed for major crops in the world, although not for every crop. However, they are also applicable to other crops not mentioned in the standard. For example, in order to provide adequate guidance, it is necessary to mention the equipment used in the production of seeds, but this does not mean that the equipment is considered to be superior and can produce similar products. Scope
National Standard of the People's Republic of China
Testing Procedure for Forage Seeds
Germination Test
Rnleg for forage seed testing-Cermination test
This standard specifies the methods and procedures for seed germination test. GB/T2930.4—2001
B 2930 =982 Chapter 5
This standard is applicable to the germination test of forage seeds, forage seeds and forage crop seeds:? Reference Standards
The provisions contained in the following engraved pieces shall become the provisions of this standard through the application of this standard. When this standard is out of print, the versions shown are valid: All parties to this standard shall discuss the possibility of using the latest version of the following standards. /T2930.1-2001 Quick Chapter Seed Inspection Rules GB123:5022001 Forage Seed Inspection Riding Heat Process Purity New G[5/3170-1987 Stimulation Value Revision Rules
3 Inspection Purpose
The maximum germination rate of the bud test is determined by the month of the test. The quality of different batches can be compared, and the seed price can also be estimated. 4 Specifications
This standard adopts the market to determine the terminology of the state record A4 (standard record).M.1 Emission rate erminetinn|| tt||After the experimental seeds develop to a certain stage, the main structure of the seedlings in this stage indicates whether they can grow into normal seeds under suitable conditions.
4.2 Percentage of normal seedlings produced in the experiment under the conditions and time specified in Table 1 is the percentage of the number of qualified seeds. 4.3 The main structure of the seedlings produced in the experiment is the main structure of the seedlings, including the embryo, bud, leaf and embryo sheath (mainly and). Appendix (Appendix of the standard description)
4.4il: NCRmal grccinga
Under good soil and other water, light and light conditions, the normal seedlings should meet one of the following types!
4.4.1 Seedlings intactseedl.ir.ps
Seedlings are mainly good in growth, and the whole training and age are in the bed. The National Quality and Technical Supervision Bureau approved the implementation of 20010314 200106-01
GB/T2930.4—2001
4.4.2 Seedlings with slight defects have slight defects in the structure of the seedlings, but in other aspects, they can be compared with the three-year-old seedlings, and are equivalent to the intact seedlings in the test. 4.4.3. Secondary infection of the teeth due to infection by bacteria or bacteria, the onset of summer disease and rot in the teeth, but there is no requirement for the original old sleep heart to seek the wide variety of teeth, in accordance with the provisions of 1.4.1 yuan 1.4.2.
The detailed description of the relevant market activities and their main structures is shown in Appendix A (Appendix to the Standard). 4.5 Abnormal animals: animals that cannot continue to grow and develop normally under good conditions of good soil conditions and poor nutrition. The abnormal conditions include the following types: 4.5.1 Damaged animals: animals with incomplete structures due to various external factors, which are seriously damaged and cannot grow normally. 4.5-2 Abnormal shapes: growth failure or physiological disorders, or asymmetrical structures. 4.5-3 Detayedtedling: caused by infection (disease from the plant), which causes the structure to rot and develop, and the growth of the animals is normal. For a detailed description of the abnormal conditions, please refer to Appendix 22 of the standard. 4.6 Single-planted seed units
can produce more than one effective seedling. For details, see Appendix A3 of the standard). 4.7 Ungerminaiedecs
Seeds that cannot germinate under the specified conditions and during the test period. They are divided into the following types: 4.7.1 Hard seeds
Seeds that cannot absorb water during the test and remain hard throughout the test period: 4.7.2 Fresh seeds
Seeds that are stable during the test period but can develop into effective seeds after being kept at a certain temperature. 4.7.3 Dead seeds
Neither fresh seeds nor any seeds. 4.7.4 The seeds are completely empty or have some residual group. 1.7.5 The seeds have no endosperm or ectodermal tissue, and have no embryo cavity and viscera. 4.7.6 The seeds have no insects, insects or internal damage, and the germination ability has been affected. 5 Apparatus and materials 5.1 The seeds usually need to be treated with acid as the germination agent (see Table 1). Under special conditions, it can also be used as the germination agent. The water quality of the hair bed should be pure, dead and poisonous, with no active II value of t~7.534
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GB/T 2930.4 2001
table! Planted germination square ditch
poured
15-25**
3 --8b3u---30
23--U -2.
20--.0,..--2..
115 ~22
120 ~a0...-2.
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GB/T 2930.4: 20C1
I: (continued)
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2035:25--30
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No supervisory voucher
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Start with the miscellaneous flower festival belt!
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TP 20--33:15--25
11120.-- 31: 15--25
F times main fire
number count
with instructions
21RNO,
16：KNO,
71: 4NO
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5 1 --
: 4 10
(30-.35 net G
10 Dongganhezi
[20 --35;16--25+ 1 5/1.
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GH/T 2930. 4-2001
Table 1 (Sui)
Origin Area
20--33
2C --35:20-- 30
2G· -35
20~-30
TP[3 2G--3:20-0
20 --30
30--30:15--25
TP.B2C-- 30:20
25-30:15--25
2-30:15-25
2--39:15--25
T2G:25
TPC--25
PP2C-- J.
TP15-- K:15--25
TP10--25
TP20--3℃wwW.bzxz.Net
EPBP20
23-- 30 ,:5--251
15~-25120
20--35120-- 30
2~-35110-35
10~-35-20·36
TPIB26--3u 20
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Effective Effective
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2e JSO KNO
4--7 23
Precooling KN,
Precooling: KNG
Prepurification; KNO
21Precooling KNO
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6-- 6 141
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10 creation net: Sichuan border second sea installation tight correction
4114 no need
514 pre-cooling
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GB/T 2990.42001
Table 1 end)
germination Kang
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same liquid technology disease
room widely allowed to use the hair with, the test escort asked and I except the rest out of the treatment method disease exchange at the end of the hair bed Sichuan the same, its currency female times, can be used to call the sample time plus instructions
list said the month to order change \2C0" all day read a total of 16
effective, cut time to use the bed value The formula is about the time. If you choose a lower temperature, the effect can be improved. Come and try to change the design. See the instructions for the seeds that need light. The true meaning of the shrinkage is as follows: Filling: Black water, Sichuan brand water; Heart A: - next year's Lingyou my long reservoir water! H.SO),: Before the experiment, first condense the seeds in the dyeing gel, 5. 1-1 secretion bed can be naturally lacking, water-absorbing lacking door as bed 5. .1. 1—Stock Requirements
5.1.1.1.1 The fiber content of the paper shall be 0.000, and the fiber spacing of the paper shall be 0.000. The fiber spacing of the paper shall be 0.000. The fiber spacing of the paper shall be 0.000. The fiber spacing of the paper shall be 0.000. The fiber spacing of the paper shall be 0.000. The fiber spacing of the paper shall be 0.000. The fiber spacing of the paper shall be 0.000. The fiber spacing of the paper shall be 0.000. The fiber spacing of the paper shall be 0.000. The fiber spacing of the paper shall be 0.000. The fiber spacing of the paper shall be 0.000. The fiber spacing of the paper shall be 0.000. 5.1.1.1. Disinfection: Paper should be disinfected before use to eliminate the harmful substances that may have been generated. 5.1.2.1.2.1.2.1.2.1.2.1.2.1.2.1.2.1.2.1.2.1.2.1.2.1.2.1.2.1.2.1.2.1.2.1.2.1.2.1.2.1.2.1.2.1.2.1.2.1.2.1.2.1.2.1.2.1.2.1.2.1.2.1.2.1.2.1.2.1.2.1.2.1.2.1.2.1.2.1.2.1.2.1.2.1.2.1.2.1.2.1.2.1.2.1.2.1.2.1.2.1.2.1.2.1.2.1.2.1.2.1.2.1.2.1.2.1.2.1.2.1.2.1.2.1.2.1.2.1.2.1.2.1.2.1.2.1.2.1.2.1.2.1.2.1.2.1.2.1.2.1.2.1.2.1.2.1.2.1.2.1.2.1.2.1.2.1.2.1.2.1.2.1.2.1.2.1.2.1.2.1.2.1.2.1.2.1.2.1.2.1.2.1.2.1 This time the card root submerge frequency, sometimes vibrate sharp color, the root from the paper, the hair into song: in the different disciplines, the tooth pin will be flat 38
sharp short.
5.1.2
5-1-2-1--GB/T 2930. 4--7001
5.1.2.1.1 composition, the size of the particles should be uniform without micro-kang and large particles, all sand particles should be above the sieve of .8m, and above the sieve of .05m. The sand should not contain any harmful substances that may affect the growth of the seedlings, lack certain fungi, or have any supporting substances. 5.1.2.1.2 Water retention: When adding an appropriate amount of water, the sand should have enough water to maintain sufficient moisture. This ensures that the water is sufficient to provide sufficient space and ventilation for the growth of the seedlings and the growth of the seedlings, thus ensuring good germination of the seeds and normal root growth. 5.12.1.3aH: The minimum H of the sand should be 6.0-7. 5.1.2.1.4 Disinfection: Before use, the yarn should be disinfected. 5.1.2.1.5 Reuse: Before use, the yarn should be disinfected. The yarn should be treated with chemical treatment and no longer used. 5.1.2.2 Determination of properties In order to ensure that the new use does not contain substances, the determination should be carried out according to the method of determining the properties of paper (see 1, 1.1.2). 5.3 Preparation 5.1.3.1 Teeth: Good quality, no inspection, no particles, no seeds or substances that affect the growth of seeds and seedlings 5.1.3.2 Water holding capacity: When the water content reaches the appropriate level, the water content should be kept at a proper level to facilitate the growth of the roots. 5.1.3.32 II: The pH value should be 6i,0--7.5. 5.1.3.4 Shang: The environment should be cleared by the sun. 5.1.3.5 Electric recovery: 5.2 Several instruments: Several boards, several boards, vacuum punches, etc. 5.3 Issuing equipment: Jacob Bo Materials (also bell-shaped or can be opened. 1) Raising box: adjustable light.
c) Raising room: temperature and light service: d, empty bud blood, bud blood culture E) Raising tray, etc. 5.4 Necessary equipment
a) Refrigerator:
5) Low box room.
c! Electric heating network
nitric acid, with acid nitric acid, sell again acid.
6 Inspection procedures
5.1 Inspection sample
From the fully cleaned seed T92. Take 00 short seeds. Pass with 10 0 seeds are counted as one replicate. If the seeds are infected with pathogens, 0 seeds need to be divided into 2 replicates. If 2 seeds are counted as one replicate, the unit can purchase a single tax booklet for testing without the need for price correction. 6.2 Test conditions
The conditions specified in Table 1 include: germination, etc., according to the flag, and continuous germination of new seeds. 6.2.1 Germination beds
The suitable germination beds for various seeds are determined according to 1 Zhao. The seeds are collected from the paper bed and the seeds are fried in the bed. 3
Sichuan uses various germination beds.
GB/T 7930.4-2051
When using paper to identify encephalopathy products, if the paper bed is contaminated and needs to be tested, the results should be determined in Table 1. If the paper bed is not tested once, if necessary, the seedlings should be placed in the sand bed. When the seedlings are infected or not, soil bed can be used for comparison of the seedling bed. 6.2.1.1 Paper bed
Unpack the paper bed, paper and paper bottle: 6.2.1.1.1 Paper bed (TP): Place the seeds on a layer or a layer of paper for seedlings. The paper can be placed on a transparent box or culture medium. When the test is started, add an appropriate amount of water and add or use an electric device on the culture medium to reduce the seedlings to the same level:
! Place the seeds directly on the seed tray or on the seed tray of the seedling pin or seedling table. The relative humidity in the box or the study should be as close to 90% as possible to prevent dryness. Wet loose paper or polyester cotton can be used as a guide for seed growth. S.2.1.1.2 Paper space (EP) Place the seeds between two layers of paper for germination. One of the following methods can be used: a) Place the seeds on a layer or two layers of filter paper, and then add another layer of filter paper on top; c) Place the seeds in the paper, hold the paper towel upright in the germination box, and use a plastic bag for the germination pots. Place the seeds directly in the germination box. The relative humidity in the box should be as close to 90% as possible. 6.2.1.1.3 (F: The paper shape is folded and has 59 parts. Press each piece of paper to wrap the entire paper strip. , put them in a box or directly in a "wet base\sticky", this method can replace TP or BP method 6-2.1.2 seconds
including sand and broken doors.
6-2.1.2.1 75 6.2.1.2.2 5, sow the seeds on the ground and cover them with ~1m thick secret broken doors. The thickness of the sand depends on the size of the seeds. In order to ensure good air circulation, it is best to dry the ground as much as possible before sowing. 6.2.2. Water and degassing
The first watering of the charcoal bud bed should be zero during the root period. The size of the bud bed depends on the type and size of the seeds. Add water to add more water (small and medium-sized snake seeds are pieces, and the grains are as follows: remove the excess water; if you use a soil bed, add water until the soil sticks to the hand, and then press it gently with your fingers to form a ball. Do not use a soil bed. The purpose is to form a layer of water film around the planting. ||tt| | The storage room should be kept moist: when adding water, try to avoid re-signing and training. The difference between the test method and the test method can also be used. The germination lead should be covered with a water tray and other moisturizing measures. During the development period, ventilation should be noted: P and HP test methods do not need to be used. The test should be fairly loose, and there should be enough air in the sand and soil bed. The test materials should not be tight. 6.2-3 Temperature || tt || The temperature of the germination test should be in accordance with the provisions of Table 1. The temperature of the germination test should be consistent with the temperature of the germination test source, and the temperature change should not exceed +1. The specified flow rate is the upper limit. When the temperature is changed under the moonlight or artificial light, it should be noted that the temperature should not exceed the standard temperature. When the temperature is changed, it is usually kept at a low temperature for 6 hours. Non-resting version, can be gradually changed within 1 hour, should be 1 or more time to do a sharp temperature change or move the test method to a lower temperature. In the tooth box. Because the temperature cannot be adjusted, the test was carried out under low temperature conditions. 6.2.4 North
Table! The species can be grown under light or darkness, but it is recommended to use light period, which can promote the growth of bacteria and make identification easier. In addition, the growth of small plants under natural conditions is weak, so the path of the plant is relatively sensitive to nature. In addition, the lack of chlorophyll can be observed.
GM/T 2930.42001
Some information is given below (some are sensitive to light and some are effective), light can promote dormancy and development! 6.1.1), but in one case, only a few effective species should be slightly different because it can be used to make germination with light or black muscle. See Table 1\Additional instructions\Part,
6.2.5 Selection of method
When there are several optimal methods in Table 1, one of the methods (combinations of germination bed and temperature) should be selected. The selection of the method depends on the equipment and experience of the inspection station, the time ratio and the source and condition of the sample. If the only method that can not send calcium technology samples, the other methods should be selected and retested. 6.3 Cultivate on a slippery bed according to the requirements of Work 1. Place the seeds evenly on a wet bed in a place where the seeds are collected. The seeding equipment should be kept at a high temperature to reduce the radiation of the seeds. The cultivation should be carried out according to the standards in Table 1. The temperature should be checked during the germination period to avoid air pollution. If there are infected seeds, wash them and replace the infected germination bed. 6.1 Treatments that have not been promoted due to various reasons (such as trisomy, hardness, and resistance to infection) can be used to collect fresh seeds that have been stored for several days. The test can be repeated after a one-time treatment or a combination of treatments or treatments. If you have any concerns about the use of these special treatment methods at the beginning of the test, 6.4.1 Except for the treatment of seeds with a long dormancy period, only the samples must be placed in a cold flow before the treatment. Place all varieties in a moist germination environment for 5-1:1. The treatment time is up to 1:1. The pre-germination time must be extended. G4.1.3 Heat before planting: Heat at 35°C for a treatment time of up to 1:1 and then proceed to germinate according to the specified conditions. In some cases, there is a need to increase the pre-germination time. In some cases, there is a need to increase the pre-germination time. For seeds with a long dormancy period, the pre-germination time can be increased by 6.4.1.4. When germinating, heat at 81°C for a treatment time of up to 1:1. During the test period, the light intensity is about 75~125c×cold light lamp. 6.4.1.5 Nitrate (KN: When the test starts, moisten the specimen with water for 2 minutes, and then add water to moisten it. .2 Monitor the concentration of KN: Put KNO3 in 1 water. 6.d.1. Acid G should be applicable (r) Heg and Secee), etc., use GA for treatment. The liquid is wetted and the bed is moistened. The standard is 0.02. The deep one can be used. 1. When preparing A solution, when the concentration is less than or equal to 8%, use water to prepare it. When the concentration is greater than 8%, use water to prepare it. For example, prepare G solution. Mix 51\4, molten! 1!. Buffalo and mix it to 0.1% G4 solution. Mix 100r with 11. Strong harmonic solution. The preparation of the molten solution is to dissolve 1.9 sodium hydrogen phosphate in hot water.
6.4.1-Case 7: When the test is completed, there is still a high proportion of fresh unripe seeds (such as the new seeds should be sealed in the environment of large-scale energy consumption). The seeds can often be exposed. 6.4.2 Methods for removing hard seeds
During the germination test, the seeds containing seeds are usually not removed. The rate of germination can be determined immediately. Some special treatments are required for the determination of the rate of germination. The seeds can be quickly germinated and reversed before the test. However, in order to avoid adverse effects on non-expected seeds, the seeds with hard seeds can also be discarded during the test period. 6.4.2.1 Note: Seeds containing seeds can be germinated quickly after germination. The germination period can reach 228h. 6.4.2.2 Seed breaking: Be careful to pierce the seed coat, raise the teeth or grind the seed coat. You can also use a needle to pierce the leaf or a half-blade to cut the leaf (leaf separation). The test product should be placed in a suitable position close to the seed part of the strong end of the ten leaves. 4h4.1 Except for the seeds with a long dormancy period, the samples must be placed in a cold flow before treatment. Place the seeds in a moist germination environment for 5-1:1. The treatment time is 1:1. The temperature in the room is specified for normal germination. The following are some instructions. The pre-germination time must be extended. G4.1.3 Heat before planting: Heat at 35°C for 1:10 + 2:00 ℃ and then perform germination according to the specified conditions. In some cases, the pre-germination time may be extended. The seeds with such heat accumulation can be used. 6.4.1.4 When germinating, heat at 81°C. During the test period, the light intensity is about 75~125c×cold light lamp. 6.4.1.5 Nitrate (KN: When the test starts, moisten the specimen with water for 2 minutes, and then add water to moisten it. .2 Monitor the concentration of KN: Put KNO3 in 1 water. 6.d.1. Acid G should be applicable (r) Heg and Secee), etc., use GA for treatment. The liquid is wetted and the bed is moistened. The standard is 0.02. The deep one can be used. 1. When preparing A solution, when the concentration is less than or equal to 8%, use water to prepare it. When the concentration is greater than 8%, use water to prepare it. For example, prepare G solution. Mix 51\4, molten! 1!. Buffalo and mix it to 0.1% G4 solution. Mix 100r with 11. Strong harmonic solution. The preparation of the molten solution is to dissolve 1.9 sodium hydrogen phosphate in hot water.
6.4.1-Case 7: When the test is completed, there is still a high proportion of fresh unripe seeds (such as the new seeds should be sealed in the environment of large-scale energy consumption). The seeds can often be exposed. 6.4.2 Methods for removing hard seeds
During the germination test, the seeds containing seeds are usually not removed. The rate of germination can be determined immediately. Some special treatments are required for the determination of the rate of germination. The seeds can be quickly germinated and reversed before the test. However, in order to avoid adverse effects on non-expected seeds, the seeds with hard seeds can also be discarded during the test period. 6.4.2.1 Note: Seeds containing seeds can be germinated quickly after germination. The germination period can reach 228h. 6.4.2.2 Seed breaking: Be careful to pierce the seed coat, raise the teeth or grind the seed coat. You can also use a needle to pierce the leaf or a half-blade to cut the leaf (leaf separation). The test product should be placed in a suitable position close to the seed part of the strong end of the ten leaves. 4h4.1 Except for the seeds with a long dormancy period, the samples must be placed in a cold flow before treatment. Place the seeds in a moist germination environment for 5-1:1. The treatment time is 1:1. The temperature in the room is specified for normal germination. The following are some instructions. The pre-germination time must be extended. G4.1.3 Heat before planting: Heat at 35°C for 1:10 + 2:00 ℃ and then perform germination according to the specified conditions. In some cases, the pre-germination time may be extended. The seeds with such heat accumulation can be used. 6.4.1.4 When germinating, heat at 81°C. During the test period, the light intensity is about 75~125c×cold light lamp. 6.4.1.5 Nitrate (KN: When the test starts, moisten the specimen with water for 2 minutes, and then add water to moisten it. .2 Monitor the concentration of KN: Put KNO3 in 1 water. 6.d.1. Acid G should be applicable (r) Heg and Secee), etc., use GA for treatment. The liquid is wetted and the bed is moistened. The standard is 0.02. The deep one can be used. 1. When preparing A solution, when the concentration is less than or equal to 8%, use water to prepare it. When the concentration is greater than 8%, use water to prepare it. For example, prepare G solution. Mix 51\4, molten! 1!. Buffalo and mix it to 0.1% G4 solution. Mix 100r with 11. Strong harmonic solution. The preparation of the molten solution is to dissolve 1.9 sodium hydrogen phosphate in hot water.
6.4.1-Case 7: When the test is completed, there is still a high proportion of fresh unripe seeds (such as the new seeds should be sealed in the environment of large-scale energy consumption). The seeds can often be exposed. 6.4.2 Methods for removing hard seeds
During the germination test, the seeds containing seeds are usually not removed. The rate of germination can be determined immediately. Some special treatments are required for the determination of the rate of germination. The seeds can be quickly germinated and reversed before the test. However, in order to avoid adverse effects on non-expected seeds, the seeds with hard seeds can also be discarded during the test period. 6.4.2.1 Note: Seeds containing seeds can be germinated quickly after germination. The germination period can reach 228h. 6.4.2.2 Seed breaking: Be careful to pierce the seed coat, raise the teeth or grind the seed coat. You can also use a needle to pierce the leaf or a half-blade to cut the leaf (leaf separation). The test product should be placed in a suitable position close to the seed part of the strong end of the ten leaves. 4h
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