This standard specifies the test principle, test equipment, test samples, test procedures, test cycle, test result evaluation and test report content of mildew-proof packaging. This standard applies to the test method of the impact of mildew on the packaging and packaging materials of products during circulation and the degree of impact. This standard does not apply to the test method of the impact of mildew on the packaging and packaging materials of food, medicine and other products during circulation and the degree of impact. GB/T 4857.21-1995 Test method for mildew prevention of packaging and transport GB/T4857.21-1995 Standard download decompression password: www.bzxz.net
This standard specifies the test principle, test equipment, test samples, test procedures, test cycle, test result evaluation and test report content of mildew-proof packaging. This standard applies to the test method of the impact of mildew on the packaging and packaging materials of products during circulation and the degree of impact. This standard does not apply to the test method of the impact of mildew on the packaging and packaging materials of food, medicine and other products during circulation and the degree of impact.

National Standard of the People's Republic of China
Test Method for Mould-proof Packaging for Packaging and Transport
Packageag-Transport packages-Mould-proof packaging test method1 Subject content and scope of application
GB/T4857.21--1995
Replaces GB4769--84
This standard specifies the test principle, test equipment, test samples, test procedures, test cycle, test result evaluation and test report content of mould-proof packaging.
This standard applies to the test method for the impact of mould on the packaging and packaging materials of products during circulation and the degree of impact. This standard does not apply to the test method for the impact of mould on the packaging and packaging materials of food, medicine and other products during circulation and the degree of impact.
2 Reference standards
GB4122 General terms for packaging
GB/T4768 Mould-proof packaging
3 Terms
The terms of this standard conform to the definitions in GB4122. 4 Test Principle
This test method is to conduct the test in a test box (room) designed according to the physiological characteristics of fungus growth and simulating the environment of natural fungus growth, so as to assess the ability of packaging or packaging materials to resist the invasion of toxic fungi. 5 Test Equipment
This test should be conducted in a special fungus test box (room), and the mold test box (room) should meet the following technical requirements. 5.1 The temperature of each point in the effective space of the test box (room) should be between 28 and 30°C, and the temperature of the indicating point should be controlled at 29±1°C. 5.2 The relative humidity of the effective test space in the test box (room) should be controlled to be greater than 96%, and the relative humidity of the indicating point should be controlled at 97±1%. 5.3 The temperature fluctuation of each point in the effective space of the test box (room) shall not exceed 1°C per hour. 5.4 The test box (room) should be ventilated once every 7 days. During the ventilation period, the temperature in the box (room) shall not be lower than 25℃, and the relative humidity shall not be lower than 80%. The temperature at the indicating point is allowed to fluctuate between 25 and 32℃, but after the ventilation is completed, it shall reach the values ​​specified in Articles 5.1 and 5.2 within 2 hours. 5.5 The wind speed in the test box shall be between 0.5 and 2m/s, but the wind speed shall not affect the normal growth of each bacteria and meet the requirements of Article 5.1.
6 Test Procedure
This test must be conducted by professionals who master the microbiological operation technology. 6.1 Preparation of Test Samples
6.1.1 Test samples can be selected according to the requirements of product labeling and environmental conditions during the circulation process. Packages or representative packaging components, simulation parts, inner packaging and packaging materials can be used for anti-mildew performance testing. 6.1.2 The selected sample should be consistent with the final form of the product and can represent the packaging process of the package. 6.1.3 During the whole process of preparing the test sample, it must be ensured that the test sample is not contaminated by human beings. 6.1.4 When the test sample selected is packaging material, the number of each type shall not be less than 3 pieces. 6.1.5 When the test sample selected is a large sample such as a packaging or packaging component, the number of samples shall be determined by negotiation between the test unit and the test unit.
6.2 Preparation of test control samples
6.2.1 The control samples of the test are divided into control test samples for detecting spore viability and control test samples for determining the test conditions in the test box. 6.2.2 The control test samples for detecting spore viability are single spore suspensions of various strains used in the test, mixed into solid culture medium, and cultured in a constant temperature incubator at 25℃. If various strains grow well within 7 days, the test is valid. 6.2.3 The control sample for determining the test conditions in the test box is a pure cloth strip or paper piece containing liquid culture medium. The samples prepared in 6.1 are randomly placed in the test box. If the mold of the control sample grows well, the test is valid. Otherwise, the test must be repeated. 6.3 Preparation of test bacteria
6.3.1 The following eight bacteria should be used in the test of mildew-proof packaging. If the packaging material or special packaging is tested, the corresponding bacteria with corrosive ability can be added, but it must be noted in the report. Aspergillus niger
Aspergillus terreus
Aureobasidium pullulans ArnandPaecilomyces variot Bamier
Penicillium funiculostm ThomPenicillium ochrochloron BiourgeScopulariopsis breuicaulis ThomTrichoderma virde Pers.Ex. Fx.6.3.2 The bacteria used in the test must be fresh strains that have been re-cultivated for 14 to 21 days. 6.4 Preparation of spore suspension
(A. C 3. 392 8)
(A. C 3. 393 5)
(A, C 3. 398 4)
(A, C 3. 425 3)
(A. C 3. 387 2)
(A. C 3. 430 2)
(A. C 3. 398 5)
(A. C 3. 294 2)
The preparation of spore suspension should be carried out according to the microbiological operation procedures. Use the strains specified in 6.3.1. Inoculate the re-cultured spores into 200 mL of sterile water and add 1 drop of wetting agent (Tween 60 or Tween 80). The number of spores of each strain shall not be less than 0.5×105/mL. No water droplets shall be produced when the sample is sprayed with spore suspension. 6.5 Test conditions
6.5.1 The mold box for the test shall be a test equipment that meets the technical index requirements of the mold box. 6.5.2 During the test of the mold test box, the temperature at each point shall be between 28 and 30°C, and the relative humidity shall be greater than 96%. 6.5.3 During the test, the air shall be changed once every seven days. 7 Test cycle
The test cycle is generally 28 days. If the degree of damage to the test sample by the bacteria is to be determined, the test cycle shall be extended to 84 days, but it must be clearly specified in the product standard.
8 Test steps
8.1 Debug the test box according to the requirements of 6.5.2 until the temperature and humidity in the box are stable and meet the requirements. 8.2 Randomly hang the test samples specified in 6.1 and the test control samples specified in 6.2 in the box. 8.3 Spray the spore suspension prepared in 6.4 evenly on the samples in the box. 8.4 If a simulated piece or non-sealed package is used for the test, the inner package must be infected with streptococcal spores. If a normal package or sealed package is used, it is not necessary to infect the mold spores in the package seal. 334
GB/T 4857.21—1995
8.5 Close the test box door and start counting the test time. Perform a comprehensive inspection at the end of the 28d or 84d cycle. 9 Nesting Assessment
9.1 After the test, the test samples must be tested according to the requirements of the mildew-proof packaging grade of GB/T4768, and the length of the sample and the area of ​​mildew growth must be recorded in detail.
9.2 For the mildew-growing samples, the reasons for the growth of mildew must be analyzed and the basis for the test analysis must be provided. 10 Test report
10.1 In order to complete the test report and facilitate the analysis of the test results, the following documents should be available before the test is carried out; a.
Source of samples, sample delivery method (delivery or random inspection), quantity and production batch number, etc., design, structure and packaging process of packaging, according to the requirements of mildew-proof packaging level specified in GB/T4768: if the sample has been treated with mildew-proof, the anti-encapsulation treatment process should be provided, 10.2 The test report should include the following items: a.
Name, source and quantity of packaging;
Name and model of mildew test box used, test conditions, temperature and relative humidity value: test cycle,
Name of test strain and increase or decrease of strain, description of mildew growth status and record of mildew growth degree, analysis, conclusion and suggestions:
Signature of test operator and auditor,
Stamp of unit. www.bzxz.net
Additional notes:
This standard is proposed by the Ministry of Electronics Industry of the People's Republic of China. This standard is under the jurisdiction of the Standardization Research Institute of the Ministry of Electronics Industry. This standard was drafted by the Fifth Research Institute of the Ministry of Electronics Industry. The main drafters of this standard are: Chen Tongshan, Zhang Zheng, Zhong Yaoming, Shao Huiqin, and Wang Zhong.
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