This standard specifies the determination method of acesulfame potassium in beverages. This standard is applicable to the determination of acesulfame potassium in soft drinks, cola-type beverages, fruit juices, fruit teas and other foods. This standard is applicable to the determination of saccharin sodium. The detection limit of this standard: acesulfame potassium and saccharin sodium are 4μg/mL (g) respectively. The linear range of acesulfame potassium and saccharin sodium is 4μg/mL~20μg/mL respectively. GB/T 5009.140-2003 Determination of acesulfame potassium in beverages GB/T5009.140-2003 Standard download decompression password: www.bzxz.net

National Standard of the People's Republic of China
GB/T5009.140—2003
Replaces GB/1634—1996
Determination of acesulfarne K in beverages
Promulgated on 2003-08-11
Ministry of Health of the People's Republic of China
Standardization Administration of China
Implementation on 2004-01-01
CB/T 5009. 140—2003
This standard replaces GBT16345
1996 Determination of acesulfarne potassium in beverages
This standard has modified the structure of the original standard in accordance with GB/T2001.4—2UH≤Rules for the preparation of standards Part 4: Chemical analysis methods>.
The standard was proposed by the Ministry of Health of the People's Republic of China and drafted by: Food Hygiene Inspection Institute of the Ministry of Health, Beijing Municipal Epidemic Prevention Station, Institute of Environmental Health, Chinese Academy of Medical Sciences.
The main contributors of this standard are: Zu Ying, Zhang Pingwei, Sun Fu, Yao Cuiyuan, Zheng Lan. This standard was first issued in 1996 and this is the first revision. 2 yuan
1 English mouse
Determination of potassium acetylsulphonate in beverages
This standard specifies the determination of potassium acetylsulphonamide in beverages. This standard is applicable to the determination of potassium acetylsulphonate in foods such as soda, cola-type beverages, fruit juices and teas. This standard is also applicable to the determination of auxiliary sodium.
GR/T5009.140—2003
The detection limits of this standard are: 4 HR/mT.1.F> for potassium acetylated sodium and sodium sulfonate, respectively; 1μ/ml.~20μ/mT.
2 The potassium acetyl sulfonate in the sample is separated by reverse phase C: phase, and the retention time is used for qualitative analysis, and the peak height or the peak value is determined by the concentration. 3 Reagents
3.1 Formaldehyde,
3.2 Ethyl acetate.
3.3.02 mol/1.2 Ammonium hydroxide: weigh 2.642 g of silica gel: add water to dissolve to 1930 ml. 3.410 Dilute the solution.
3.5 Neutral alumina chromatography with 10C ~ 230 days, 3.6 Ethylsulfonamide potassium, sodium arginine standard solution: take 100 ml of acetylcarbamide potassium and 100 ml of each in molten steel, dissolve with mobile phase and transfer to 100 ml narrow bottle, and use moving phase shift to dilute to the desired solution, that is, 1 mg/ml of potassium sulfonate as the base, 3.7 Ethylsulfonamide potassium and sodium arginine standard solution, use plated, absorb Take 2mL of potassium acesulfame and sodium sulfonate standard solution in a 511mL volumetric flask, add the mobile phase to the mark, then absorb 1.2ml..3ml), 4ml..5ml of this solution in 10mL volumetric flasks and add the mobile phase to the mark. You can get a series of mixed standard filters containing potassium acesulfame and sodium sulfonate 4ug/mL..5ug/mL.12g/mL, 16g/mL, and 20g/mL respectively.
3.8 Mobile phase: 0.C2mnL/1.5L HCl (40~-800) + ethanol (170~150) + ethyl acetate (90~50) - 1 (% HSCh (1m1.). 4.2 Ultrasonic clarifier (for flow purifier). 4.3 Centrifuge.
4.4 Twist-type bottle.
4.53 Acid-resistant funnel.
4.6 Porous membrane 0.45L
4.7 Chromatographic column, can be replaced by 1C raL injection cartridge + 1 cn high neutral oxidation. 5 Analysis steps
5.1 Sample treatment
5.1.1 Soda: Warm the sample, stir to remove carbon dioxide or flue gas. Absorb 2.Eml of sample in 25ml empty volume, add mobile phase, mix well, and filter through microporous filter for HPLC analysis. 5.1.2 Cola-type material: Warm the sample, stir to remove carbon dioxide and flue gas, and remove the magnesium oxide 2.5mJ.205
GR/F5009,140-2003
When the sample reaches the surface of the filter, flow through the filter, collect 25ml of eluent, and use FPLC to separate the product gas. .
5.1.3 Polymerization, fruit juice food
Absorb 2.5 μm of sample, add about 20 ml of water. After filtering, high centrifuge 15 tia4000x/min), reduce all the lead oxides on the surface, wait for the water to flow to the surface, and then wash with mobile phase. Collect 25 mL of elution, and after a while, ultrasonically degas: this is for HPLC analysis.
5.2 Preparation
5.2.1 HPLC test conditions
Analysis t: ssheisarht.dsmmx15umm, particle size 5pnl, follow-up book 002mol/l value cavity (740mL~800ml1+ (17m15mT) Japanese encephalitis (90mL~EC mI,) 1 G%H.NO,T mL).
Capacity: 214012
Flow rate: 0.7L/.
5.2.2 Standard curve: The sample contains 4A/mL of potassium acetylated colloid, 4g/mL of sodium saccharin, 12g/L, 16g/ml2u/m1. Mix the standard solution, and then perform ITC to separate the standard. Then, the peak area is used as the standard, and the content of 7.5% potassium acetylated colloid and saccharin is used as the coordinate to draw the standard.
5.2.3 Sample determination Take 12L of the sample treated in 5. and perform HLC analysis. Determine the peak surface and use the standard to obtain the content of 7.5% potassium acetylated colloid and saccharin sodium in the sample. HPLC chromatographic conditions 1
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Product result calculation
The amount of acetic acid in the sample is calculated by the following formula: XX.VXL000
mX1500
Wherein:
The content of acetic acid in the sample is expressed in grams or milligrams per liter (mR/k or g-liter). The amount of acetic acid in the sample is expressed in grams or milliliters (g/.206).
The total amount of sample dilution is expressed in milliliters (mL): The maximum amount of the sample is expressed in grams or milliliters (g or g). The calculation results shall retain two significant figures, and the absolute difference between two effective determination results obtained under repeatability conditions shall not exceed 11% of the arithmetic mean.
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