This standard specifies the technical requirements, test methods and inspection rules for β-cyclodextrin. This standard applies to β-cyclodextrin obtained by enzyme conversion of starch and then purification with water. It is used as a stabilizer, flavoring agent, shape-modifying agent, etc. for food. QB 1613-1992 Food Additive β-Cyclodextrin QB1613-1992 Standard download decompression password: www.bzxz.net

Industry Standard of the People's Republic of China
Food Additives
β-Cyclodextrin
QB1613-92
This standard applies to β-cyclodextrin obtained by enzyme conversion of starch and then purification with water. It is used as a stabilizer, flavoring agent, shape-modifying agent, etc. in food.
Molecular formula: (C6H1005)7
Molecular weight: 1134.997 (according to the international atomic weight in 1983) Technical requirements
1.1 Sensory requirements
This product is a white crystalline powder, odorless, slightly sweet. 1.2 The physical and chemical indicators of β-cyclodextrin should meet the requirements in the table. Item
Content (in total sugar)
1Indicator, %
Heavy metal (in terms of Pb)≤
Arsenic (As)
2Test method
2.1Identification
≤0.0001
Test method
IGB5009.4
GB8451
IGB8450
2.1.1Weigh 1g of sample and add 100mL of water. After mixing, take 1mL into a test tube, add a drop of trichloroethylene, shake vigorously, and produce a white precipitate.
2.1.2Weigh 1g of sample and add 100mL of water. After mixing, take 1mL and drop it on a white porcelain plate, add a drop of 0.02mol/L iodine solution, and it will show a light yellow color.
2.2 Content and total sugar determination
2.2.1 Principle
The total sugar content of the sample is determined by the phenol-sulfuric acid method. The characteristic of saccharifying enzyme that only decomposes linear dextrin and oligosaccharides but not cyclodextrin is used. The amount of glucose produced by saccharifying enzyme acting on the sample is measured by the DNS method (3.5-dinitrosalicylic acid method). The ratio of the difference between the total sugar and the amount of glucose to the total sugar is the content of cyclodextrin. 2.2.2 Reagents and solutions
a. Sulfuric acid (GB625);
b. Phenol (HGB3131, redistilled): 80% aqueous solution; c. 0.2mol/L acetic acid-sodium acetate buffer solution: (PH4.5) Take 11.43mL glacial acetic acid (GB676) and dilute to 1000mL to form an acetic acid solution with a concentration of 0.2mol/L. Weigh 27.2g sodium acetate (GB693) or 16.4g anhydrous sodium acetate (GB694) and dissolve to a fixed volume of 1000mL to form a sodium acetate solution with a concentration of 0.2mol/L. Mix the solution in a ratio of acetic acid solution: sodium acetate solution = 5.7:4.3 (volume ratio). The pH value of the above buffer solution should be calibrated with an acidometer; d. Glucoamylase solution: Prepare a solution of Rhizopus glucoamylase (for laboratory use) with an enzyme activity of 4U/m L, prepared when needed, can be stored in a refrigerator at 4℃, the storage period does not exceed 3 days; e. 3,5-dinitrosalicylic acid solution Weigh 12.6g of 3,5-dinitrosalicylic acid, 364g of potassium sodium tartrate (GB1288), 10g of redistilled phenol, 41.92g of sodium hydroxide (GB629), and 10g of sodium bisulfite (HG3-1291), mix and heat to dissolve, and then dilute to 2000mL; store in a dark place for one week, filter with filter paper for later use. This solution is referred to as DNS solution: f. Glucose standard solution: weigh 0.5000g of glucose (HG3-1094), dissolve it in water, dilute to 500mL, and become a 1000ug/mL glucose standard solution; absorb 10mL of the above standard solution, dilute to 100mL, and then become a 100μg/mL glucose standard solution.
2.2.3 Determination Procedure
2.2.3.1 Determination of Glucose
a. Preparation of glucose standard curve by DNS method. Prepare the reaction solution in a test tube according to the table.
1000μg/mL glucose standard solution mL/0.00/0.10/0.15|0.20/0.30|0.40|0.60/0.80|1.00Www.bzxZ.net
Content of glucose in colorimetric solution
Acetic acid-sodium acetate buffer
DNS solution
mL|1.50|1.40|1.35|1.30|1.20|1.10|0.90|0.70[0.50μg/mL
1 100|1501200[300[4001600|800|+
11.011.0|1.0
mL|2.52.5|2.52.5|2.52.5[2.52.5|2.5Place the above reaction solution in a boiling water bath and react for 5 minutes. Then take it out and cool it to room temperature. Use a 0.5 cm colorimetric cup on a spectrophotometer to compare colors at a wavelength of 540 nm. Record the extinction value. Draw a standard curve with glucose content as the horizontal axis and extinction value as the vertical axis.
b. Determination of the amount of glucose produced by enzymatic hydrolysis in the sample Weigh 1.000g of sample, dissolve it to a constant volume of 100mL, draw 1mL of the sample solution, add 1mL of 0.2mol/L acetic acid-sodium acetate buffer (PH4.5). Then add 0.5mL of glucose amylase solution, place it in a 50℃ super constant temperature water bath for reaction for 30min. When the reaction is terminated, add 2.5mL of DNS solution, place it in a boiling water bath for 5min, then take it out, cool it to room temperature, and determine the extinction value according to 2.2.3.1a. Replace the sample solution with 1mL of water as a blank. Check the standard curve according to the extinction value of the sample to obtain the amount of glucose in the colorimetric solution. According to formula (1), calculate the amount of glucose A (g) produced by enzymatic hydrolysis in 1g of sample. CX100
Where: A——the amount of glucose produced by enzymatic hydrolysis in 1g of sample, g; 2.2.3.2 Determination of total sugar
The value found on the standard curve, ug. a. Preparation of glucose standard curve by phenol-sulfuric acid method. Prepare the reaction solution in a test tube according to the table. Reagents
100μg/mL glucose standard solution
Glucose content in colorimetric solution
Benzenzyme (80% aqueous solution)
【1.111.3
Note: Mix the three solutions in the table and then add sulfuric acid. When adding sulfuric acid, the pipette should be facing the liquid surface and flushed straight down, with 5 drops of acid remaining on the pipette wall as the standard. After the above reaction solution is cooled to room temperature, use a 0.5cm colorimetric cup on a spectrophotometer to compare colors at a wavelength of 490nm, record the extinction value, and use the glucose content as the horizontal axis. The extinction value is the ordinate, and the standard curve b is drawn. Determination of total sugar in the sample
Dilute the sample solution in 2.2.3.1b 100 times to make a 100ug/mL sample solution. Take 1mL of the sample solution, add 1mL
water and 50mL phenol aqueous solution, shake well, and then add 5mL sulfuric acid. After the reaction, cool to room temperature and determine the extinction value according to 2.2.3.2a.
Use 2mL of water to replace the sample solution.
According to the extinction value of the colorimetric solution, check the standard curve to obtain the total sugar content of the colorimetric solution. According to formula (2), the total sugar content β (in terms of glucose g) in 1g of sample is calculated. C×100×100
Wherein: B-
-1g total sugar content (in glucose), g; 2.2.3.3 Calculation
-×100
Wherein: X
-β-cyclodextrin content (in total sugar), %; β
-1g total sugar content (in glucose), g. A
Value found on the standard curve, ug. ·(3)
-1g glucose content produced by enzymatic hydrolysis, g;
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