This part mainly specifies the methods and basic requirements for field trial of insecticide glue for controlling cotton blind bugs. This part is applicable to the field efficacy trial and efficacy evaluation of insecticides for controlling cotton blind bugs. Other field efficacy trials shall refer to this part. NY/T 1464.3-2007 Guidelines for field efficacy trials of pesticides Part 3: Insecticides for controlling cotton blind bugs NY/T1464.3-2007 Standard download decompression password: www.bzxz.net
This part mainly specifies the methods and basic requirements for field trial of insecticide glue for controlling cotton blind bugs. This part is applicable to the field efficacy trial and efficacy evaluation of insecticides for controlling cotton blind bugs. Other field efficacy trials shall refer to this part.

ICS65.100
Agricultural Industry Standard of the People's Republic of China
NY/T 1464.32007
Guidelines on efficacy evaluation of pesticidesPart 3: Insecticides against cotton plant bug2007-1218 issued
2008-03-01 implementation
The Ministry of Agriculture of the People's Republic of China issued
NY/T1464 "Guidelines for the Test of Pesticide Efficacy" is a series of standards, with a total of 26 parts: Part 1: Insecticides for the control of migratory locusts;
Part 2: Insecticides for the control of rice water weevils; Part 3: Insecticides for the control of cotton brooding stink bugs;
Part 4: Tea industry agents for the control of pear yellow aphids: Part 5: Insecticides for the control of apple aphids; Part 6: Insecticides for the control of vegetable schizonts; Part 7: Fungicides for the control of tobacco anthracnose; - Part 8: Fungicides for the control of tomato virus diseases; Part 9: Fungicides for the control of pepper disease; Part 10: Fungicides for the control of mushroom wet bubble disease; Part 11: Fungicides for the control of banana black spot; - Part 12 : Fungicides for controlling powdery mildew of grape; - Part 13: Fungicides for controlling anthracnose of grape; - Part 14: Fungicides for controlling damping-off of rice; - Part 15: Fungicides for controlling wheat chain disease; - Part 16: Fungicides for controlling wheat root rot; - Part 17: Herbicides for controlling weeds in mung bean fields; - Part 18: Herbicides for controlling weeds in sesame; - Part 19: Herbicides for controlling weeds in elm and wolfberry fields; - Part 20: Herbicides for controlling weeds in tomatoes; - Part 21: Herbicides for controlling weeds in cucumber fields; - Part 22: Herbicides for controlling weeds in sorghum fields; - Part 23: Herbicides for controlling weeds in fields; - Part 24: Herbicides for controlling weeds in adzuki beans; - Part 25: Herbicides for controlling weeds in tobacco seedbeds; - Part 26: Test for cotton wilt accelerators.
This part is Part 3 of the "Test Methods for Intermolecular Efficacy of Pesticides". This part was proposed and managed by the Department of Crop Production Management, Ministry of Agriculture of the People's Republic of China. The drafting unit of this part is: Pesticide Control Institute, Ministry of Agriculture. The main drafters of this part are: Xia Xiaojun, Ma Yan, Pan Wenliang, Tao Lingmei, Ji Lili, Sak Fu, Xu Lirui. NY/T 1464.32007
1 Scope
Guidelines for field efficacy tests of pesticides
Part 3: Insecticides for controlling cotton stink bugs
NY/T1464.32007
This part specifies the methods and basic requirements for field efficacy plot tests of tea insecticides for controlling cotton stink bugs (mainly including green wax bug (Lygtslucorum), middle black stink bug (Adelpmocorissuturatis), first stink bug (Adelphocorislinenleatus), three-spotted first stink bug (Adelphocorisfusciaticuttis) and pasture stink bug (Lygus pratensis)". This part is applicable to the field efficacy plot tests and efficacy evaluation of tea insecticides for registered use for controlling cotton stink bugs. Efficacy tests for other uses shall refer to this part.
2 Test case
2.1 Test object and crop
The test object is cotton. Record the main cotton varieties, advantages and groups and the percentage of cotton blind threads in the test area, record the main development stages of the dominant varieties during the test,
The test crop should be the main local variety. Record the variety name, growth period, and planting density.2.2 Test site
The test should select a production cotton field with a relatively uniform density of cotton stink bugs and representative damage. The cultivation conditions of the test site (such as soil type, fertilization, tillage, watering, etc.) must be uniform and consistent and meet local good agricultural practices. 3 Experimental design and arrangement
3.1 Experimental treatment
3.1.1 Agents
3.1.1.1 Experimental agents
The experimental agents shall be set with 3 or more treatment doses, or set according to the requirements of the agreement: record the generic name (in Chinese or English) or code, dosage form, content, manufacturer and treatment dose (expressed as active ingredient/hm). 3.1.1.2 Control agents
Control agents must be registered agents that have been proven effective in practice. Their types and modes of action shall be similar to those of the experimental agents. Control agents shall be used at the local conventional application rate. If the experimental agent is a single agent, at least one local commonly used single agent shall be set as the control agent; if the experimental agent is a mixture, the single agent in the mixture shall be used as the control agent, and the local commonly used agent shall also be included as the control agent. Record the Chinese generic name, dosage form, content, manufacturer and application rate of the control agent. 3.1.2 Blank control
Set no pesticide treatment as the blank control.
3.2 Repetitions
Each treatment shall be repeated at least once.
3.3 Plot arrangement
3.3.1 Plot area
The plot area shall not be less than 10m2, preferably 40m2 to 60m2, and 5 to 6 rows of cotton plants shall be guaranteed. 1
NY/T 1464. 3---2007
3.3.2 Plot arrangement
Each plot shall be arranged in random blocks: record the plot arrangement diagram. 3.4 Requirements for pesticides used to control pests other than the test object If other pesticides are needed to control diseases, insects and weeds other than the test object during the test, pesticides that have no effect on the test pesticide and the test object should be selected, and they must be used separately from the test pesticide and the control pesticide, and all test plots shall be treated equally. Record the accurate data of the application of such pesticides (name of pesticide, content, dosage form, manufacturer, application dosage, application method, application time). 4 Application of pesticides
4.1 Application method
Perform according to the requirements of the agreement, and the application should comply with local good agricultural practices. 4.2 Application equipment
Use common equipment for application according to the dosage form of the test pesticide, or perform according to the requirements of the agreement. Record the type and operating conditions of the application equipment. 4.3 Application time
Perform according to the requirements of the agreement, or determine according to the appropriate period of cotton breeding control. The control period is based on the control indicators of dominant species, taking into account other species. Generally, when the number of cotton stink bugs per plant reaches 4-6 heads in the seedling stage, the number of insects per plant reaches 10-12 heads in the bud stage, and the number of insects per plant reaches 20-25 heads or more in the boll stage, the monthly number of insects continues to increase. It is necessary to choose to apply the pesticide in the evening and record the time (date) of application. 4.4 Application times
Carry out according to the agreement requirements and record.
4.5 Application amount
Determine the application amount according to the application method, crop growth period, and plot area, or carry out according to the agreement requirements. Record the application amount, expressed in L/h.
5 InvestigationwwW.bzxz.Net
5.1 Effect investigation
5.1.1 Investigation method
Sampling was conducted at 5 points on the diagonal line of each plot, and 10 to 20 cotton plants were continuously inspected at each point, and the total number of plants was marked with a sign), and the number of adults and nymphs of cotton bugs on each cotton plant was recorded. According to the nocturnal rhythm of adults and nymphs of the Buddha bug, it is advisable to choose to conduct the investigation when the insects are not active. 5.1.2 Survey time and frequency
Survey the base number before application (number of live insects before application, number of damaged plants before application), and investigate the number of residual insects (number of live insects after application) on the 1st, 3rd and 7th day after application. The number of surveys can be increased or the survey time can be extended according to the test requirements and the characteristics of the agent. During the last survey after the application, it is necessary to investigate and record the number of cotton plants in each test plot that are damaged by cotton blind valley (number of damaged plants after application), or investigate and record the number of flower buds that have fallen off and the total number of flower buds of the listed cotton plants in each test plot (usually investigate the number of damaged plants, typical damaged plants or broken leaves at the end of the fungus period or the early stage of bud appearance; investigate the total number of flower buds and the number of flower buds that have fallen off at the peak of thunderstorm period): 5.2 Direct impact on crops
Observe whether the pesticide has any lethal damage to the crops. If there is any damage, record the symptoms, type and degree of damage. In addition, the beneficial effects on crops (such as accelerated maturity, increased vitality, etc.) should also be recorded: Record the year of the drug use in the following manner:
- If the drug damage can be counted or measured, it should be expressed in absolute values, such as plant height, etc. - In other cases, the degree and frequency of drug damage can be estimated in the following two ways: 1) According to the drug damage classification method, record the drug damage situation in each plot, and express it in terms of one, ten, twenty, ten, eleven, and so on. Drug damage classification method:
- No drug damage;
Ten: Mild drug damage, visible damage, no obvious impact on crop growth; Ten: Obvious drug damage, can be recovered, and will not cause crop yield reduction; NY/T1464.3—2007
11: High pesticide damage, affecting the growth of crops, causing a certain degree of loss in crop yield and quality, and generally requiring compensation for part of the economic losses;
1-10: Severe pesticide damage, crop growth is hindered, crop yield and quality loss is serious, and economic losses must be compensated. 2) Compare the pesticide treatment area with the control area, evaluate the percentage of pesticide damage, and at the same time, accurately describe the pesticide damage symptoms of the crop (dwarfing, chlorosis, deformity, etc.), and provide physical photos, videos, etc. 5.3 Impact on other organisms
5. 3. 1 Impact on other pests and diseases
Any impact on other pests and diseases should be recorded, including beneficial and unbeneficial impacts. 5.3.2 Impact on other non-target organisms
Record the impact of the pesticide on wild organisms and beneficial insects in the test area. 5.4 Other data
5.4.1 Meteorological data
Collect and record the meteorological data of the test site during the entire test period, including rainfall (fall type and daily rainfall, expressed in mm) and temperature (average temperature, maximum and minimum temperature, expressed in °C). 5.4.2 Soil data
Collect and record the soil fertility, crop yield level, and crop or weed coverage of the ten soil types. 6 Calculation formula and data analysis
Calculate the control effect according to formula (1), calculate the new infected plant rate and the control effect according to formula (2) and formula (3), calculate the chemical shedding rate and the thinning effect according to formula (4) and formula (5), and apply the biostatistics method (DMR method) for statistical analysis. PT
Where:
Wu Zhong:
Where:
Control effect, unit is percentage (%); Number of live insects in the treatment area before application, unit is head; Number of live insects in the treatment area after application: unit is head; Number of live insects in the control area before application, unit is head! Number of live insects in the control area after application, unit is head. P
Rate of new damaged plants, unit is white fraction (%): Number of damaged plants after application, unit is plant;
Number of damaged plants before application, unit is plant;
Total number of plants, unit is plant.
CK X PT
CK,X FT,
.n3×100
Pz = CK Pz ×100
PZ is the damage effect, in white fraction (%): the rate of new damaged plants in the pesticide treatment area, in white fraction (%); PZa
CK is the rate of new damaged plants in the blank control area, in percentage (%). (1)
NY/T 1464. 3-2007
Wherein:
the rate of flower bud shedding, in white fraction (%): the cumulative number of flower bud shedding, in pieces;
the total number of flower buds investigated, in pieces.
Wu Zhong,
Bao effect, unit is white fraction (%):
CKPLax i00
1L-Bud shedding rate in the drug treatment area, unit is percentage (%): CK.-
Bud shedding rate in the blank control area, unit is percentage (%). Drug evaluation and report writing
Analyze and evaluate the drug according to the results, write a formal test report, and list the original data. (4)
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