This standard specifies the detection method of Pseudomonas aeruginosa in experimental animals. This standard is applicable to the detection of Pseudomonas aeruginosa in mice, rats, guinea pigs, hamsters and rabbits. GB/T 14926.17-2001 Detection method of Pseudomonas aeruginosa in experimental animals GB/T14926.17-2001 Standard download decompression password: www.bzxz.net

ICS 65.020.30
National Standard of the People's Republic of China
GB/T 14926.17-2001
Laboratory animal
Microbiological exaration methods (2)
Laboratory animalMicrobiological exaration methods2001-08-29Promulgated
General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China
Implementation on 2002-05-01
GB/T14926-17-2001
This standard is a revision of GB/T14926.17-1PP46 Laboratory animal Green bacillus test method. This standard adds "635 solid dynamic test chamber" and 6.3.6 42C growth test and 5-test sequence -. The intensity of the serum type is determined, and the direct type identification is only used when necessary, so the flag is removed and the "5-test" is added. The positive oxidation test is an important biochemical indicator of the vaccine. In the single-matching vaccine, the amount of green pigment produced by the bacillus is calculated. When green pigment and the problem are produced, the oxidation test can be directly judged by Gram staining. This standard is issued by the People's Republic of China and is under the jurisdiction of the Ministry of Science and Technology of China. The drafting unit of this standard is the Chinese Society of Laboratory Animals, and the main drafter of the standard is the East China Standard Edition
This standard was first issued in January 2019
1 Scope
National Standard of the People's Republic of China
Laboratory Animals
Pseudomonas aeruginosa Detection Method
Labaeatory animal-Method fi essminatian ntPstndomgugs serrglaosg
This standard specifies the experimental animals
This standard applies to mice·
2-The reference standard solution
is valid until the standard is broken. There are standards
GB/T14926
GB/T1492643
3 Abdominal care
Fengliangxuanyuan is a
basis road breeding, Jijian insurance
4 Main equipment materials 4.1 General culture medium and 2 Biological conditions, 5 Culture equipment for culture of bacillus-free bacteria, passed the test of 1-Experimental laboratory test, GB/T 14926.17-2001, CB/T14926-17-1594, this standard is the version shown at the time of publication, the standard may be revised. ||tt| ...
Basalt culture medium
People's Republic of China National Quality Supervision and Inspection Commission 2001-08-29 Batch 2002-05-01 Commercial
Inspection sequence
Green pigment and questions
7 Operation steps
7-1 Sampling
GB/T14926-17—2001
NAC chain culture medium
Baotong unloading car
2h Green pigment
Non-self-production
18-24 h
No dry color required, see sauce title
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Take the guest of Ju Yan Fei, the brand will be empty Nan Du map now, divide the help. 7-2 separation culture
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will have been authorized 8C reduction stop culture world base set 36 Wang 1 heart culture world 18-24h store beautiful growth please product, obstacle production chapter old old sharer transfer NAC item level II, (36±1)C culture 18~24h. 7.3 Adjustment
7.3.1 Growth characteristics Colony characteristics
Rhizoctonia solani grew in NAC medium in a uniform manner: on the NAC medium, it formed flat colonies with a diameter of more than 2-3 mm and no serrations on the edges. Most of the colonies could produce green pigment, causing the medium to appear green, and some bacteria could only produce pigment after long-term cultivation. Gd
7.3.2 Physical characteristics
Gram-positive bacilli, different bacterial species
7.3.3 Oxidase test positivebzxz.net
GB/T 14926.172001
The tested surface is a gram-positive bacilli, oxidase positive and produces bee color, which can be reported as a test bacillus, 7.3.4 Biochemical characteristics
For the bacilli that do not produce explosive color, the following should be determined, see fee 1, the main biochemical characteristics of green bacilli
Guangzhou
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7-3.5 Semi-motility test positive.
42C growth test positive.
Fruit with sound
Food waist melting
Clear cavity search chemical
If the above violations are found, a positive report will be made, and the results will not be reported. The test results will not be reported. The test results will be reported as positive. The test results will not be reported as positive. The test results will be reported as positive. The test results will be reported as positive. The test results will be reported as positive. The test results will be reported as positive. The test results will be reported as positive. The test results will be reported as positive. The test results will be reported as positive. The test results will be reported as positive. The test results will be reported as positive. The test results will be reported as positive. The test results will be reported as positive. The test results will be reported as positive. The test results will be reported as positive. The test results will be reported as positive. The test results will be reported as positive. The test results will be reported as positive. The test results will be reported as positive. The test results will be reported as positive. The test results will be reported as positive. The test results will be reported as positive. The test results will be reported as positive. The test results will be reported as positive. The following should be provided: see fee 1, the main biochemical characteristics of green with the bacteria
blue country essence
Guangzun environment
ten, sex
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positive
7-3.5 semi-solid dynamic test positive
2C growth test positive
culture report
acyl salt
clear cavity
several lines of close speed various tests need to make a positive report, unequal combination of the required period of sex, digestion
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