Some standard content:
GB 18416—2001
Technical requirements 4.1, 4.3, 4.7, 4.8, and 4.9 of this standard are mandatory, and the rest are recommended. The household hygienic insecticide coil mosquito coil is a product with pyrethroids and other types of hygienic insecticides as active ingredients. After ignition, the agent acts on mosquitoes in a gaseous state to drive away (fire) mosquitoes. This standard is formulated on the basis of the light industry standard QB1692-1—1993 "Household hygienic insecticide coil mosquito coil" of the People's Republic of China1, and appropriate modifications and additions are made to the contents of each chapter to make the standard more standardized and the content more complete. At the same time, the appendix A and appendix B of the standard explain the determination method of the active ingredient in detail, which is applicable to the test needs of different columns of gas chromatograph capillary column and packed column: Appendix C of the indoor efficacy determination method of coil mosquito coils cites GB13917.4! 992 "Test Method for Indoor Efficacy of Pesticide Registration for Sanitary Use of Tapeworm Agents and Indoor Efficacy Determination Method of Mosquito Coils", and re-specified the temperature in the test room in combination with actual conditions. This standard will replace QB1632.1-1993 from the date of implementation. Appendix A, Appendix B and Appendix C of this standard are all appendices of the standard. This standard was proposed by the China Light Industry Federation. This standard is the second of the National Standardization Center for Miscellaneous Goods. The drafting units of this standard are: Nanjin City Mosquito Coil General Factory, Hunan Yiyin City Ant Coil General Factory, Hebei Gucheng Incense Industry Group Co., Ltd., Shanghai Samsung Supplies Company, and the main drafters of this standard are: Li Jingfeng, Qin Xiaoming, Yang Jinqing, Sun Changwei, and Wei Xiaoying. 690
1 Scope
National Standard of the People's Republic of China
Domestic sanitary insecticide-Mosquito coil incensc
Domcstic sanitary insecticide-Mosquito coil incensc
GB 18416—2001
This standard specifies the definition, technical requirements, test methods, inspection rules and marking, packaging, transportation, storage and instructions for use of household sanitary insecticide-mosquito coil incensc
This standard applies to household sanitary insecticide, plant powder, carbonaceous powder, adhesive and colorant mixed with other raw materials.
2 Referenced Standards
The provisions contained in the following standards constitute the provisions of this standard through reference in this standard. When this standard is published, the versions shown are valid. All standards will be revised, and the parties using this standard should explore the possibility of using the latest versions of the following standards. GB/T2828-1987Batch-by-batch inspection, sampling procedure and sampling table (applicable to the inspection of continuous batches) G13/T28291987 Periodic inspection, sampling procedure and sampling table (applicable to the inspection of the stability of the production process) General provisions for the use of consumer products
G13 5296. 1--1597
GB13917.4-1992 Pesticide registration. Indoor efficacy test method for raw insecticides Indoor efficacy determination method for mosquito coils 3 Definitions
This standard adopts the following definitions.
3.1 Knockdown time (KT50 value)
Under the specified conditions, the time required for 50% of the test insects to be knocked down (i.e., lying on their backs): 3.2 Efficacy
The product should achieve the desired repellent (killing) effect under the specified conditions and within the specified time. 3.3 Active ingredients
Chemical ingredients with biocidal properties, 3.4 Ignition cabinet
A device that can make the sample under test in a place without the influence of external airflow, 3.5 Flatness
Degree of warping of the plane of the disc mosquito coil
3.6 Continuous burning time
The time from the start of ignition to the burning out of the single-circle disc mosquito coil. 3.7 Bending strength
The ability of the single-circle disc mosquito coil to resist breaking
Approved by the General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China on August 28, 2001-rKAONiKAca-
Implemented on July 1, 2002
4 Technical requirements
4. 1 Requirements for medication
GB 18416 2001
Must be a drug registered or approved for use by the relevant national government departments. 4.2 Appearance and sensory perception
4.2.1 Appearance: The incense body is clean and tidy, with uniform color, no mold spots on the incense surface, and no breakage, deformation or defect on the incense strip. 4.2.2 Sensory perception: No irritating odor.
4.3 Continuous burning time
The continuous burning time of each single coil mosquito coil is 71, and it shall not be extinguished in the middle (the special specifications of the coil mosquito coil shall be in accordance with the burning time requirements of Minger). 4.4 Bending strength
The bending strength of each single coil mosquito coil shall be N
4.5 Debonding
Except for the connection point, the other parts of the incense body are easy to be completely detached. 4.6 Flatness
The flatness of the incense body shall meet the test requirements of 5.6. 4.7 Efficacy
KT507min.
4.8 Active ingredient content
The active ingredient content of the product must be clearly stated on the packaging 1 and shall not be lower than the content clearly stated on the product. 4.9 Thermal stability
After the thermal storage stability test, the degradation rate of the active ingredient content of pyrethroids shall be ≤5%, and the degradation rate of the active ingredient content of other types shall be ≤10%. 5 Test method
5.1 Usage requirements
Check whether there is a registration or identification certificate for the drug. 5.2 Appearance and sensory properties
Use visual inspection and film sense method. Under normal light, pick up the sample at a distance of 250mm. 5.3 Continuous burning time
5. 3. 1 Test conditions
Room temperature: (26+3)C
Relative humidity: (65±10)%
5.3.2 Take 10 sheets of the sampled product and place them under the above test conditions for 24h. Then place them in the ignition cabinet and ignite them. Record the continuous time from ignition to extinction.
5.4 Flexural strength
5.4. 1 Test conditions
Room temperature (26→3)C
Relative humidity: (5±10)%
5.4.2 Pack and package them and place them under the above test conditions for 24h before testing. Use the single-circle outer load method, tie the innermost circle of the sample to the frame, take three equally spaced points on the outermost circle, tie a 250mm long thin line and hang a 102g magnetic code, let it stand for 15s, the incense body should not break, 5.5 Ring-off
The connection point of the open disc mosquito coil is used to gently push the two sections in the center of the mosquito coil from opposite sides, then pinch the two ends with your fingers, loosen slightly, and gradually break into two single pieces, the incense body should not break. 5.6 Flatness
GB 18416- 2001
Use two pieces of transparent flat glass with a length of 150mmi and a width of 135mm to form a parallel card with a spacing of 8mm. The double-circle disc mosquito coil can pass naturally in the card,
5.7 Efficacy
The determination method is shown in Appendix C.
5.8 Active ingredient content
The determination method is shown in Appendix A and Appendix B, where Appendix A is the arbitration method; for products that are not applicable to the active ingredient analysis method of this standard, the manufacturer shall provide the analysis method.
5.9 Thermal stability
Put the test sample (with packaging) in a constant temperature box at (51=2) for 14 days, take it out, and then measure its degradation rate according to the active ingredient content method.
5.9.1 The degradation rate is calculated according to formula (1): Where A
Degradation rate;
A(%)=2=m×100
The effective ingredient content of the sample measured before heating; the effective ingredient content of the sample measured after heat storage, 6 Inspection rules
6.1 The product must be inspected and qualified by the quality inspection department of the production factory according to this standard before it can be shipped out of the factory, and it must be accompanied by instructions for use and inspection certificates. 6.2 Inspection is divided into delivery inspection and type inspection
6.2.1 Factory inspection
All products proposed for delivery shall be inspected. Factory inspection adopts (B/2828 Special Normal inspection of inspection level S-3 No. 1 sampling plan, qualified quality level AQL value: Class B is 6.5. Class C is 10, out! Inspection items and unqualified classification are shown in Table 1. Table 1
6.2.2 Type inspection
Inspection items
External period and inductive force
Continuous ignition point
Flexural strength
Uncirculation
Smoothness
Technical requirements clauses
6.2.2. 1 Type inspection should be carried out in any of the following situations, test method clauses
a) Trial determination and identification of new products or old products transferred to the factory for production: b) After formal production, if there are major changes in structure, materials, and processes that may affect product performance, c) During normal production, sample inspection of batch products shall be carried out at least times a year: d) Product discontinuation:More than half a year, when cattle production resumes; e) Out! When the inspection result is significantly different from the last type inspection: Unqualified classification
f) When the national quality supervision and inspection agency condenses the type inspection requirements. 6.2.2.2 Type inspection adopts the one-time sampling plan of G-13/T2829 discrimination level I. Its inspection items, unqualified quality level RQ1. value, sample size and judgment array are shown in Table 2. Each single circle is judged as a single figure unqualified A. R, expressed as the number of unqualified single circles, if one item is not graded in the type inspection, it is comprehensively judged as unqualified, aa
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Medicine requirements
Effective city separation and combination
Hot storage stability
Inspection items date
Appearance and filterability
Smoothness
Bending resistance
Decollation
Continuous ignition point
Marking, packaging, transportation, storage, instructions for use 7.1 Marking
GB18416—2001
Unqualified classification
Size of sample vehicle
No drug registration or identification certificate, judged as unqualified c
Below the value indicated on the package, judged Determined as unqualified 10 double plaques
10 double circles
10 single circles
Judgment array
Repeat the test three times, each time randomly select two small sections from different packages, KT50>7min is determined to be unqualified
Ester>5% is determined to be unqualified
Degradation rate
Other categories>10% is determined to be unqualified
7. 1.1 The following Chinese content should be on the product packaging box: a) Product name, trademark, factory name, factory address: 6) Active ingredients and content:
c) Product standard number:
d) Production: date and shelf life or production batch number and expiration date: e) Product quality inspection certificate:
】Specifications and quantity.
7.1.2·The packaging of products shall include the following contents in Chinese: a) Pictorial mark;
b) Product name and specification:
e) Quantity;
d) Gross weight (kg);
e) Production date and shelf life or production batch guide and expiration date; f) Manufacturer's name and address;
g) Overall dimensions: length x width x height (cm)
7.2 Packaging
Product packaging must be firm, unbreakable, moisture-proof and shock-proof. 7.3 Transportation
Products should be handled lightly during transportation to prevent severe vibration, sun exposure, and heavy pressure. 7.4 Storage
Products should be stored in a cool, dry and ventilated warehouse and should not be mixed with flammable and explosive items. Under the above conditions, the shelf life of the product is not less than two years.
7.5 Instructions for use
The instructions for use of the product shall comply with the provisions of GB5296.1. 691
A1 Identification test
GB 18416:2001
Appendix A
(Appendix to the standard)
Determination method for the effective ingredients of mosquito coils (I) This identification test can be carried out simultaneously with the determination of the content of the common name of the effective ingredient. Under the same chromatographic operating conditions, the relative difference between the retention time of a chromatographic peak of the sample solution and the retention time of the standard solution (common name of the effective ingredient) should be within 1% to 5%. A2 Method Summary
The sample is separated and determined by gas chromatography on a SE-54 capillary column with dibutyl phthalate or diamyl phthalate as the internal standard.
A2.1 Instrument
A2.1.1 Gas chromatograph: with hydrogen flame ionization detector. A2.1.2 Chromatographic column: 0.25mm×30m. SE-54 capillary column with internal coating. A2.1.3 Micro syringe: 10=L,
A2.1.4 Weighing bottle: 15 ml..
A2. 1. 5 Grinding stopper=angle bottle; 100 ml.
A2. 1.6 Balance: graduation value is 0. 1 mg. A2.1.7 Pulverizer.
A2.1.8 Sieve: Standard test sieve 0.154 mm (100 mm/inch). A2.1.9 German paper,
A2.1.10 Shaker.
A2.2 Reagents
A2.2.1 Internal standard, butyl phthalate, monopentyl phthalate, without interfering impurities (scope of application of internal standard: dibutyl phthalate is applicable to samples without octachlorodipropyl ether, and dipentyl phthalate is applicable to samples with octachlorodipropyl ether) A2.2.2 Solvent: acetone, methanol (analytical grade) A2-2.3 Standard products: allethrin, ruthenyl ester, etc. A2.3 Gas chromatography conditions
42. 3. 1 Temperature
A2. 3. 1. 1 Column overflow: 220°C
A2. 3. 1. 2 Vaporization temperature: 25°C
A2. 3.1.3 Detector temperature; 250,
A2.3.2 Volume flow rate (kg/cm\)
A2.3.2.1 Carrier gas: Nitrogen 1.0.
A2.3.2.2 Fuel gas: Hydrogen 0.6.
A2.3.2.3 Combustion air 0.5
The above operating conditions are typical operating parameters. According to the characteristics of different instruments, the given operating conditions can be appropriately adjusted to obtain the best effect.
A3 Preparation of standard solution
Accurately weigh about 0.03g~0.04g of the standard sample (accurate to 695
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GB 18416—2001
0.000 2) in a 15uL weighing bottle, and then weigh 0.015g~0.025g (accurate to 0.000 2g) of dibutyl phthalate or monobutyl phthalate, add 5ml of acetone, and after fully dissolved, close the hook and put it in the refrigerator for use. A4 Preparation of sample solution
A4.1 Pretreatment of samples
Take a plate of finished mosquito coils and crush them with a crusher, then pass through a 0.154 mm (100 mm/inch) standard test sieve, and the mosquito coil powder is ready for use. A4.2 Preparation of mosquito coil extract
Ding 100 ml. Accurately weigh 10 g~15 g of mosquito coil powder in a corrugated flask (accurate to 0.000 2 g, estimated content of the tested component is about 0.03 g~0.04 g). Then weigh 0.015 g~0.025 g of internal standard (accurate to 0.000 2 g), and finally add methanol-two ketone mixed solution (volume ratio 1:1), preferably with a slightly excessive amount to cover the sample, and seal it in a vibrator]: fully vibrate for 20 li, let it stand and filter for 10 ml. In a glass bottle, for gas chromatography analysis,
A5 determination
Under the chromatographic conditions of A2.3, after the instrument is stable, continuously inject the standard solution and sample solution with a micro syringe: calculate the peak area ratio of the sample (standard sample) to the internal standard in the chromatogram of each needle, wait until the peak area ratio of the two adjacent needles is basically parallel and stable, and perform gas chromatography analysis under the above conditions.
A6 Calculation
Average the measured area ratios of the sample peak to the internal standard peak in the two sample solutions and the area ratios of the internal standard peak to the standard peak in the standard solution, and calculate the content Mx correction factor of the component to be measured according to formula (A1) and formula (A2):
the content of the component to be measured,
fA×mi/meXC
x(%)f×m-/miXAtX100
wherein: A is the average value of the area ratios of the sample peak to the internal standard peak in the sample solution; 2 is the average value of the area ratios of the internal standard peak to the standard peak in the standard solution; ml is the mass of the internal standard in the sample solution·g; w is the mass of the internal standard in the standard solution,
the mass of the sample in the sample solution·g;
the mass of the standard substance in the standard solution, : the content of the standard substance, %.
Appendix B
(Appendix to the standard)
Determination method of effective ingredients in mosquito coils (II) B1 Identification test
(A1)
(A2)
This identification test can be carried out simultaneously with the determination of the content of the common name of the effective ingredient. Under the same chromatographic operating conditions, the relative difference between the retention time of a chromatographic bee of the sample solution and the retention time of the standard solution (common name of the effective ingredient) should be within 1 to 5. B2 Method summary
The sample is separated and determined by gas chromatography on an internally coated 5SE-30 packed column using dibutyl phthalate or dipentyl phthalate as the internal standard.
B2.1 Instrument
GB 18416—2001
B2.1.1 Gas chromatograph: with a hydrogen flame ionization detector B2.1.2° Chromatographic column: $3mmx.2m, glass column or stainless steel column filled with 5% SE-30 coated on Chromastrb AWDircs 80~100 mesh chromatographic twist,
B2.1.3 Micro syringe 10 μc
B2. 1.4 Weighing bottle: 15 mL.
B2. 1. 5 Grinding stopper = angle bottle; 100 ml. B2.1.6 Balance, graduation value is 0.1mg.
B2.1.7 Pulverizer.
B2.1:8 Sieve: standard test sieve 0.154 mm (100 day/inch). B2.1.9 Filter paper.
B2.1.10 Oscillator,
B2.2 Reagents
B2.2.1 Internal standard: tributyl phthalate, dibutyl phthalate, no interfering impurities (applicable range of internal standard: dibutyl phthalate is applicable to samples without octachlorodipropyl ether (S), and monoamyl phthalate is applicable to samples with octachlorodipropyl aldehyde). B2.2.2 Solvent: acetone, methanol (analytical grade), B2.2.3 Standard products: allethrin, ethynthrin, etc., B2.3 Gas chromatography conditions
B2.3.1 Temperature
B2.3. 1. 1 Column temperature: 190C.
B2. 3.1.2 Vaporization temperature: 210℃ or 250C℃. B2.3.1.3 Detector temperature: 210℃ or 250C. B2.3.2 Gas flow rate (kg/cm2).
B2.3.2.1 Carrier gas: Nitrogen 1.0.
R2.3.2.2 Fuel gas: Hydrogen 0..
B2.3.2.3 Supporting gas: Air 0.5.
The above operating conditions are typical operating parameters. According to the characteristics of different instruments, the given operating conditions can be appropriately adjusted to obtain the best effect.
Preparation of B3 standard solution
In a 15 mL weighing bottle, accurately weigh about 0.03 g ~ 0.04 g (accurate to 0.0002 g) of the standard sample equivalent to the sample to be tested, and then weigh 0.015g ~ 0.025g (accurate to 0.0002g) of dibutyl phthalate or diamyl phthalate, add 5ml of acetone solution, after fully dissolved, close the shaker and put it in the refrigerator for use. B4 Preparation of sample solution
B4.1 Sample pretreatment
Crush the finished mosquito coil with a grinder and pass it through a 0.151 m (100 mesh/inch) standard test sieve. The mosquito coil powder is ready for use. B4.2 Preparation of mosquito coil extract
Accurately weigh 10 g to 15 g of mosquito coil powder in a 100 mL stoppered conical flask (accurate to 0.000 2 g: the estimated content of the measured component is about 0.03 g to 0.04 g). Then weigh 0.015 g to 0.025 g of internal standard (accurate to 0.0002 g). Finally, add methanol-lactone mixed solution (volume ratio 1:1) with a slightly excessive amount to cover the sample. Vibrate the mixture on an oscillator for 20 minutes and filter it until it is 10 mL glass bottle for gas chromatography analysis,
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B5 determination
GB184162001
Under the chromatographic conditions in 12.3, after the instrument is stable, continuously inject the standard solution and sample solution with a micro syringe to calculate the peak area ratio of the sample (standard sample) to the internal standard in the chromatogram of each needle. When the peak area ratio of the two adjacent needles is basically parallel and stable, perform gas chromatography analysis according to the above conditions.
B6 Calculation
Average the measured sample peak to internal standard peak area ratios in the two sample solutions and the internal standard peak to standard peak area ratios in the standard solution, and calculate the content Mx correction factor of the component to be measured according to formula (BI) and formula (B2):
Amount of component to be measured:
Where: A.
f -A,Xmia/mzXCt
Mx(%)=fXm/m1XA.X10)
The average value of the sample peak to internal standard peak area ratio in the sample solution; The half-mean of the internal standard peak to standard peak area ratio in the standard solution: The mass of the internal standard in the sample solution, the mass of the internal standard in the standard solution;
The mass of the sample in the sample solution:
The mass of the standard substance in the standard solution, the content of each standard substance, %.
Appendix C
(Standard Appendix)
Method for indoor efficacy determination of mosquito coils
For testers: Use standard test mosquitoes; the female adult mosquitoes that are the second to third largest to suck blood after emergence, Culex pipiens pallens is used in northern areas, and Culex pipiens quinquefasciatus is used in southern areas. C2 Test conditions
Room temperature: (26±2)
Relative humidity: (60±5)%
C3 Instruments
(B2)
C3. 1 Closed cylinder device (see Figure C1): A glass or transparent colorless plastic cylinder (C) with an inner diameter of 20 rm and a height of 43 cm is placed on this frame (I). There are 27 cm diameter glass or transparent colorless round plates (E, F) on the upper and lower sides of the side cylinder. There is a 2 cm diameter round hole in the center of the upper round plate, which is plugged with a rubber plug (G). There is a 5 cm diameter round hole in the center of the lower round plate, which is plugged with a rubber plug (H). There is a mosquito coil rack (B) on the rubber plug (H). It is used to test the number of incense. The joints between the cylinder and the upper and lower round plates are respectively padded with rubber pads (ID,,D2) to prevent smoke leakage. 698
GB18416—2001
Figure C1 Closed cylinder device
A mosquito coil; P--mosquito coil holder: C--glass or transparent colorless plastic plate, commercial 43 cm. Inner weight 20 cm11tD1.D, rubber gasket; E--glass or transparent colorless plastic circular plate, 27 cm, with a hole of 2 cm in diameter in the center; F--glass or transparent colorless plastic circular plate, 27 cm in diameter, with a hole of 5 cm in diameter in the center; G, H--rubber stopper; I--wooden frame, 30 cm high C3.2 mosquito suction tube,
C3.3 stopwatch.
C3.4 counter.
C4 Test Steps
Use a straw to suck 20 test mosquitoes from the cage, release them from the central circular hole of the circular plate (F) below the closed cylinder, plug the rubber plug (H) and wait for the test mosquitoes to come out and resume normal activities. Take any section of the mosquito coil to be tested, place it horizontally on the mosquito coil rack, light the mosquito coil and start the timer. Smoke for 1 minute. Then remove the mosquito coil and immediately plug the rubber plug (H). Record the number of test mosquitoes knocked down at regular intervals. The test is repeated at least three times. After each test, the entire test device must be cleaned before the next test. At the same time, an air test must be carried out. If the knockdown rate and mortality rate of the air test are greater than 20%, the entire test must be repeated. C5 calculation
Calculate the three repeated test data according to the machine value method to calculate KT50.99
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GB 18416--2001
GB18416-2001 "Household sanitary insecticide disc mosquito coils" and other 4 national standards No. 1 amendment
This amendment has been approved by the State Administration of Standardization on February 10, 2003 in the document No. 9 of the National Standardization Administration of Agricultural and Light Industry [2003], and will be implemented from the date of publication.
GB18416—2001 Household Hygiene Insecticide Coil" Clause No.
Table 2 Efficacy
Table 2 Thermal Stability
4.1, 4.3, 4.74.8; 1.9 of this standard are mandatory, and the rest are recommended. KT50≤7min
The degradation rate of the active ingredient content of pyrethroids
5%, the degradation rate of the active ingredient content of other types is 10%.
**., KT50>7min is judged as unqualified,
, pyrethroids>5 is judged as unqualified
Other types>10 are judged as unqualified.
Modified to
4.1, 4.3, 4.7, 4.8, 4.9, 7.1 of this standard. 1. 7.4 and 7.5 are mandatory, and the rest are recommended. KT508.0 min.
, the degradation rate of the active ingredient content of pyrethroids is ≤10%, and the degradation rate of the active ingredient content of other types is more than 15%.
..-, KT50>8.0min is judged as unqualified. , Pyrethroids>10% is judged as unqualified
Other types>15% are judged as unqualified.Glass column or stainless steel column filled with 5% SE-30 coated on Chromastrb AWDircs 80~100 mesh chromatographic twist,
B2.1.3 Micro syringe 10 μc
B2. 1.4 Weighing bottle: 15 mL.
B2. 1. 5 Grinding stopper = angle bottle; 100 ml. B2.1.6 Balance, graduation value is 0.1 mg.
B2.1.7 Pulverizer.
B2.1:8 Sieve: standard test sieve 0.154 mm (100 day/inch). B2.1.9 Filter paper.
B2.1.10 Oscillator,
B2.2 Reagents
B2.2.1 Internal standard: tributyl phthalate, dibutyl phthalate, no interfering impurities (applicable range of internal standard: dibutyl phthalate is applicable to samples without octachlorodipropyl ether (S), and monoamyl phthalate is applicable to samples with octachlorodipropyl aldehyde). B2.2.2 Solvent: acetone, methanol (analytical grade), B2.2.3 Standard products: allethrin, ethynthrin, etc., B2.3 Gas chromatography conditions
B2.3.1 Temperature
B2.3. 1. 1 Column temperature: 190C.
B2. 3.1.2 Vaporization temperature: 210℃ or 250C℃. B2.3.1.3 Detector temperature: 210℃ or 250C. B2.3.2 Gas flow rate (kg/cm2).
B2.3.2.1 Carrier gas: Nitrogen 1.0.
R2.3.2.2 Fuel gas: Hydrogen 0..
B2.3.2.3 Supporting gas: Air 0.5.
The above operating conditions are typical operating parameters. According to the characteristics of different instruments, the given operating conditions can be appropriately adjusted to obtain the best effect.
Preparation of B3 standard solution
In a 15 mL weighing bottle, accurately weigh about 0.03 g ~ 0.04 g (accurate to 0.0002 g) of the standard sample equivalent to the sample to be tested, and then weigh 0.015g ~ 0.025g (accurate to 0.0002g) of dibutyl phthalate or diamyl phthalate, add 5ml of acetone solution, after fully dissolved, close the shaker and put it in the refrigerator for use. B4 Preparation of sample solution
B4.1 Sample pretreatment
Crush the finished mosquito coil with a grinder and pass it through a 0.151 m (100 mesh/inch) standard test sieve. The mosquito coil powder is ready for use. B4.2 Preparation of mosquito coil extract
Accurately weigh 10 g to 15 g of mosquito coil powder in a 100 mL stoppered conical flask (accurate to 0.000 2 g: the estimated content of the measured component is about 0.03 g to 0.04 g). Then weigh 0.015 g to 0.025 g of internal standard (accurate to 0.0002 g). Finally, add methanol-lactone mixed solution (volume ratio 1:1) with a slightly excessive amount to cover the sample. Vibrate the mixture on an oscillator for 20 minutes and filter it until it is 10 mL glass bottle for gas chromatography analysis,
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B5 determination
GB184162001
Under the chromatographic conditions in 12.3, after the instrument is stable, continuously inject the standard solution and sample solution with a micro syringe to calculate the peak area ratio of the sample (standard sample) to the internal standard in the chromatogram of each needle. When the peak area ratio of the two adjacent needles is basically parallel and stable, perform gas chromatography analysis according to the above conditions.
B6 Calculation
Average the measured sample peak to internal standard peak area ratios in the two sample solutions and the internal standard peak to standard peak area ratios in the standard solution, and calculate the content Mx correction factor of the component to be measured according to formula (BI) and formula (B2):
Amount of component to be measured:
Where: A.
f -A,Xmia/mzXCt
Mx(%)=fXm/m1XA.X10)
The average value of the sample peak to internal standard peak area ratio in the sample solution; The half-mean of the internal standard peak to standard peak area ratio in the standard solution: The mass of the internal standard in the sample solution, the mass of the internal standard in the standard solution;
The mass of the sample in the sample solution:
The mass of the standard substance in the standard solution, the content of each standard substance, %.
Appendix C
(Standard Appendix)
Method for indoor efficacy determination of mosquito coils
For testers: Use standard test mosquitoes; the female adult mosquitoes that are the second to third largest to suck blood after emergence, Culex pipiens pallens is used in northern areas, and Culex pipiens quinquefasciatus is used in southern areas. C2 Test conditions
Room temperature: (26±2)
Relative humidity: (60±5)%
C3 Instruments
(B2)
C3. 1 Closed cylinder device (see Figure C1): A glass or transparent colorless plastic cylinder (C) with an inner diameter of 20 rm and a height of 43 cm is placed on this frame (I). There are 27 cm diameter glass or transparent colorless round plates (E, F) on the upper and lower sides of the side cylinder. There is a 2 cm diameter round hole in the center of the upper round plate, which is plugged with a rubber plug (G). There is a 5 cm diameter round hole in the center of the lower round plate, which is plugged with a rubber plug (H). There is a mosquito coil rack (B) on the rubber plug (H). It is used to test the number of incense. The joints between the cylinder and the upper and lower round plates are respectively padded with rubber pads (ID,,D2) to prevent smoke leakage. 698
GB18416—2001
Figure C1 Closed cylinder device
A mosquito coil; P--mosquito coil holder: C--glass or transparent colorless plastic plate, commercial 43 cm. Inner weight 20 cm11tD1.D, rubber gasket; E--glass or transparent colorless plastic circular plate, 27 cm, with a hole of 2 cm in diameter in the center; F--glass or transparent colorless plastic circular plate, 27 cm in diameter, with a hole of 5 cm in diameter in the center; G, H--rubber stopper; I--wooden frame, 30 cm high C3.2 mosquito suction tube,
C3.3 stopwatch.
C3.4 counter.
C4 Test Steps
Use a straw to suck 20 test mosquitoes from the cage, release them from the central circular hole of the circular plate (F) below the closed cylinder, plug the rubber plug (H) and wait for the test mosquitoes to come out and resume normal activities. Take any section of the mosquito coil to be tested, place it horizontally on the mosquito coil rack, light the mosquito coil and start the timer. Smoke for 1 minute. Then remove the mosquito coil and immediately plug the rubber plug (H). Record the number of test mosquitoes knocked down at regular intervals. The test is repeated at least three times. After each test, the entire test device must be cleaned before the next test. At the same time, an air test must be carried out. If the knockdown rate and mortality rate of the air test are greater than 20%, the entire test must be repeated. C5 calculation
Calculate the three repeated test data according to the machine value method to calculate KT50.99
iiKAoNrKAca
GB 18416--2001
GB18416-2001 "Household sanitary insecticide disc mosquito coils" and other 4 national standards No. 1 amendment
This amendment has been approved by the State Administration of Standardization on February 10, 2003 in the document No. 9 of the National Standardization Administration of Agricultural and Light Industry [2003], and will be implemented from the date of publication.
GB18416—2001 Household Hygiene Insecticide Coil" Clause No.
Table 2 Efficacy
Table 2 Thermal Stability
4.1, 4.3, 4.74.8; 1.9 of this standard are mandatory, and the rest are recommended. KT50≤7min
The degradation rate of the active ingredient content of pyrethroids
5%, the degradation rate of the active ingredient content of other types is 10%.
**., KT50>7min is judged as unqualified,
, pyrethroids>5 is judged as unqualified
Other types>10 are judged as unqualified.
Modified to
4.1, 4.3, 4.7, 4.8, 4.9, 7.1 of this standard. 1. 7.4 and 7.5 are mandatory, and the rest are recommended. KT508.0 min.
, the degradation rate of the active ingredient content of pyrethroids is ≤10%, and the degradation rate of the active ingredient content of other types is more than 15%.
..-, KT50>8.0min is judged as unqualified. , Pyrethroids>10% is judged as unqualified
Other types>15% are judged as unqualified.Glass column or stainless steel column filled with 5% SE-30 coated on Chromastrb AWDircs 80~100 mesh chromatographic twist,
B2.1.3 Micro syringe 10 μc
B2. 1.4 Weighing bottle: 15 mL.
B2. 1. 5 Grinding stopper = angle bottle; 100 ml. B2.1.6 Balance, graduation value is 0.1 mg.
B2.1.7 Pulverizer.
B2.1:8 Sieve: standard test sieve 0.154 mm (100 day/inch). B2.1.9 Filter paper.
B2.1.10 Oscillator,
B2.2 Reagents
B2.2.1 Internal standard: tributyl phthalate, dibutyl phthalate, no interfering impurities (applicable range of internal standard: dibutyl phthalate is applicable to samples without octachlorodipropyl ether (S), and monoamyl phthalate is applicable to samples with octachlorodipropyl aldehyde). B2.2.2 Solvent: acetone, methanol (analytical grade), B2.2.3 Standard products: allethrin, ethynthrin, etc., B2.3 Gas chromatography conditions
B2.3.1 Temperature
B2.3. 1. 1 Column temperature: 190C.
B2. 3.1.2 Vaporization temperature: 210℃ or 250C℃. B2.3.1.3 Detector temperature: 210℃ or 250C. B2.3.2 Gas flow rate (kg/cm2).
B2.3.2.1 Carrier gas: Nitrogen 1.0.
R2.3.2.2 Fuel gas: Hydrogen 0..
B2.3.2.3 Supporting gas: Air 0.5.
The above operating conditions are typical operating parameters. According to the characteristics of different instruments, the given operating conditions can be appropriately adjusted to obtain the best effect.
Preparation of B3 standard solution
In a 15 mL weighing bottle, accurately weigh about 0.03 g ~ 0.04 g (accurate to 0.0002 g) of the standard sample equivalent to the sample to be tested, and then weigh 0.015g ~ 0.025g (accurate to 0.0002g) of dibutyl phthalate or diamyl phthalate, add 5ml of acetone solution, after fully dissolved, close the shaker and put it in the refrigerator for use. B4 Preparation of sample solution
B4.1 Sample pretreatment
Crush the finished mosquito coil with a grinder and pass it through a 0.151 m (100 mesh/inch) standard test sieve. The mosquito coil powder is ready for use. B4.2 Preparation of mosquito coil extract
Accurately weigh 10 g to 15 g of mosquito coil powder in a 100 mL stoppered conical flask (accurate to 0.000 2 g: the estimated content of the measured component is about 0.03 g to 0.04 g). Then weigh 0.015 g to 0.025 g of internal standard (accurate to 0.0002 g). Finally, add methanol-lactone mixed solution (volume ratio 1:1) with a slightly excessive amount to cover the sample. Vibrate the mixture on an oscillator for 20 minutes and filter it until it is 10 mL glass bottle for gas chromatography analysis,
-TKAONiKAca-
B5 determination
GB184162001
Under the chromatographic conditions in 12.3, after the instrument is stable, continuously inject the standard solution and sample solution with a micro syringe to calculate the peak area ratio of the sample (standard sample) to the internal standard in the chromatogram of each needle. When the peak area ratio of the two adjacent needles is basically parallel and stable, perform gas chromatography analysis according to the above conditions.
B6 Calculation
Average the measured sample peak to internal standard peak area ratios in the two sample solutions and the internal standard peak to standard peak area ratios in the standard solution, and calculate the content Mx correction factor of the component to be measured according to formula (BI) and formula (B2):
Amount of component to be measured:
Where: A.
f -A,Xmia/mzXCt
Mx(%)=fXm/m1XA.X10)
The average value of the sample peak to internal standard peak area ratio in the sample solution; The half-mean of the internal standard peak to standard peak area ratio in the standard solution: The mass of the internal standard in the sample solution, the mass of the internal standard in the standard solution;
The mass of the sample in the sample solution:
The mass of the standard substance in the standard solution, the content of each standard substance, %.
Appendix C
(Standard Appendix)
Method for indoor efficacy determination of mosquito coils
For testers: Use standard test mosquitoes; the female adult mosquitoes that are the second to third largest to suck blood after emergence, Culex pipiens pallens is used in northern areas, and Culex pipiens quinquefasciatus is used in southern areas. C2 Test conditions
Room temperature: (26±2)
Relative humidity: (60±5)%
C3 Instruments
(B2)
C3. 1 Closed cylinder device (see Figure C1): A glass or transparent colorless plastic cylinder (C) with an inner diameter of 20 rm and a height of 43 cm is placed on this frame (I). There are 27 cm diameter glass or transparent colorless round plates (E, F) on the upper and lower sides of the side cylinder. There is a 2 cm diameter round hole in the center of the upper round plate, which is plugged with a rubber plug (G). There is a 5 cm diameter round hole in the center of the lower round plate, which is plugged with a rubber plug (H). There is a mosquito coil rack (B) on the rubber plug (H). It is used to test the number of incense. The joints between the cylinder and the upper and lower round plates are respectively padded with rubber pads (ID,,D2) to prevent smoke leakage. 698
GB18416—2001
Figure C1 Closed cylinder device
A mosquito coil; P--mosquito coil holder: C--glass or transparent colorless plastic plate, commercial 43 cm. Inner weight 20 cm11tD1.D, rubber gasket; E--glass or transparent colorless plastic circular plate, 27 cm, with a hole of 2 cm in diameter in the center; F--glass or transparent colorless plastic circular plate, 27 cm in diameter, with a hole of 5 cm in diameter in the center; G, H--rubber stopper; I--wooden frame, 30 cm high C3.2 mosquito suction tube,
C3.3 stopwatch.
C3.4 counter.
C4 Test Steps
Use a straw to suck 20 test mosquitoes from the cage, release them from the central circular hole of the circular plate (F) below the closed cylinder, plug the rubber plug (H) and wait for the test mosquitoes to come out and resume normal activities. Take any section of the mosquito coil to be tested, place it horizontally on the mosquito coil rack, light the mosquito coil and start the timer. Smoke for 1 minute. Then remove the mosquito coil and immediately plug the rubber plug (H). Record the number of test mosquitoes knocked down at regular intervals. The test is repeated at least three times. After each test, the entire test device must be cleaned before the next test. At the same time, an air test must be carried out. If the knockdown rate and mortality rate of the air test are greater than 20%, the entire test must be repeated. C5 calculation
Calculate the three repeated test data according to the machine value method to calculate KT50.99
iiKAoNrKAca
GB 18416--2001
GB18416-2001 "Household sanitary insecticide disc mosquito coils" and other 4 national standards No. 1 amendment
This amendment has been approved by the State Administration of Standardization on February 10, 2003 in the document No. 9 of the National Standardization Administration of Agricultural and Light Industry [2003], and will be implemented from the date of publication.
GB18416—2001 Household Hygiene Insecticide Coil" Clause No.
Table 2 Efficacy
Table 2 Thermal Stability
4.1, 4.3, 4.74.8; 1.9 of this standard are mandatory, and the rest are recommended. KT50≤7min
The degradation rate of the active ingredient content of pyrethroids
5%, the degradation rate of the active ingredient content of other types is 10%.
**., KT50>7min is judged as unqualified,
, pyrethroids>5 is judged as unqualified
Other types>10 are judged as unqualified.
Modified to
4.1, 4.3, 4.7, 4.8, 4.9, 7.1 of this standard. 1. 7.4 and 7.5 are mandatory, and the rest are recommended. KT508.0 min.
, the degradation rate of the active ingredient content of pyrethroids is ≤10%, and the degradation rate of the active ingredient content of other types is more than 15%.
..-, KT50>8.0min is judged as unqualified. , Pyrethroids>10% is judged as unqualified
Other types>15% are judged as unqualified.6 Balance, graduation value is 0.1mg.
B2.1.7 Crusher.
B2.1:8 Sieve: standard test sieve 0.154 mm (100 day/inch). B2.1.9 Filter paper.
B2.1.10 Oscillator,
B2.2 Reagents
B2.2.1 Internal standard: tributyl phthalate, dibutyl phthalate, without interfering impurities (the applicable scope of internal standard is solid; dibutyl phthalate is applicable to samples without octachlorodipropyl ether (S), and monoamyl phthalate is applicable to samples with octachlorodipropyl ether). B2.2.2 Solvent: acetone, methanol (analytical grade), B2.2.3 Standard products: allethrin, ethrin, etc., B2.3 Gas chromatography conditions
B2.3.1 Temperature
B2.3. 1. 1 Column temperature: 190C.
B2. 3.1.2 Vaporization temperature: 210℃ or 250℃. B2.3.1.3 Detector temperature: 210℃ or 250℃. B2.3.2 Gas flow rate (kg/cm2).
B2.3.2.1 Carrier gas: Nitrogen 1.0.
R2.3.2.2 Fuel gas: Hydrogen 0.。
B2.3.2.3 Fuel-supporting gas: 0.5% air.
The above operating conditions are typical operating parameters. According to the characteristics of different instruments, the given operating conditions can be appropriately adjusted to obtain the best effect.
B3 Preparation of standard solution
In a 15 mL weighing bottle, accurately weigh about 0.03 g ~ 0.04 g (accurate to 0.0002 g) of the standard sample equivalent to the sample to be tested, and then weigh 0.015g ~ 0.025g (accurate to 0.0002g) of dibutyl phthalate or diamyl phthalate, add 5ml of acetone solution, and after fully dissolving, close the shaker and put it in the refrigerator for use. B4 Preparation of sample solution
B4.1 Sample pretreatment
Crush the finished mosquito coil with a grinder and pass it through a 0.151 m (100 mesh/inch) standard test sieve. The mosquito coil powder is ready for use. B4.2 Preparation of mosquito coil extract
Accurately weigh 10 g to 15 g of mosquito coil powder in a 100 mL stoppered conical flask (accurate to 0.000 2 g: the estimated content of the measured component is about 0.03 g to 0.04 g). Then weigh 0.015 g to 0.025 g of internal standard (accurate to 0.0002 g). Finally, add methanol-lactone mixed solution (volume ratio 1:1) with a slightly excessive amount to cover the sample. Vibrate the mixture on an oscillator for 20 minutes and filter it until it is 10 mL glass bottle for gas chromatography analysis,
-TKAONiKAca-
B5 determination
GB184162001
Under the chromatographic conditions in 12.3, after the instrument is stable, continuously inject the standard solution and sample solution with a micro syringe to calculate the peak area ratio of the sample (standard sample) to the internal standard in the chromatogram of each needle. When the peak area ratio of the two adjacent needles is basically parallel and stable, perform gas chromatography analysis according to the above conditions.
B6 Calculation
Average the measured sample peak to internal standard peak area ratios in the two sample solutions and the internal standard peak to standard peak area ratios in the standard solution, and calculate the content Mx correction factor of the component to be measured according to formula (BI) and formula (B2):
Amount of component to be measured:
Where: A.
f -A,Xmia/mzXCt
Mx(%)=fXm/m1XA.X10)
The average value of the sample peak to internal standard peak area ratio in the sample solution; The half-mean of the internal standard peak to standard peak area ratio in the standard solution: The mass of the internal standard in the sample solution, the mass of the internal standard in the standard solution;
The mass of the sample in the sample solution:
The mass of the standard substance in the standard solution, the content of each standard substance, %.
Appendix C
(Standard Appendix)
Method for indoor efficacy determination of mosquito coils
For testers: Use standard test mosquitoes; the female adult mosquitoes that are the second to third largest to suck blood after emergence, Culex pipiens pallens is used in northern areas, and Culex pipiens quinquefasciatus is used in southern areas. C2 Test conditions
Room temperature: (26±2)
Relative humidity: (60±5)%
C3 Instruments
(B2)
C3. 1 Closed cylinder device (see Figure C1): A glass or transparent colorless plastic cylinder (C) with an inner diameter of 20 rm and a height of 43 cm is placed on this frame (I). There are 27 cm diameter glass or transparent colorless round plates (E, F) on the upper and lower sides of the side cylinder. There is a 2 cm diameter round hole in the center of the upper round plate, which is plugged with a rubber plug (G). There is a 5 cm diameter round hole in the center of the lower round plate, which is plugged with a rubber plug (H). There is a mosquito coil rack (B) on the rubber plug (H). It is used to test the number of incense. The joints between the cylinder and the upper and lower round plates are respectively padded with rubber pads (ID,,D2) to prevent smoke leakage. 698
GB18416—2001
Figure C1 Closed cylinder device
A mosquito coil; P--mosquito coil holder: C--glass or transparent colorless plastic plate, commercial 43 cm. Inner weight 20 cm11tD1.D, rubber gasket; E--glass or transparent colorless plastic circular plate, 27 cm, with a hole of 2 cm in diameter in the center; F--glass or transparent colorless plastic circular plate, 27 cm in diameter, with a hole of 5 cm in diameter in the center; G, H--rubber stopper; I--wooden frame, 30 cm high C3.2 mosquito suction tube,
C3.3 stopwatch.
C3.4 counter.
C4 Test Steps
Use a straw to suck 20 test mosquitoes from the cage, release them from the central circular hole of the circular plate (F) below the closed cylinder, plug the rubber plug (H) and wait for the test mosquitoes to come out and resume normal activities. Take any section of the mosquito coil to be tested, place it horizontally on the mosquito coil rack, light the mosquito coil and start the timer. Smoke for 1 minute. Then remove the mosquito coil and immediately plug the rubber plug (H). Record the number of test mosquitoes knocked down at regular intervals. The test is repeated at least three times. After each test, the entire test device must be cleaned before the next test. At the same time, an air test must be carried out. If the knockdown rate and mortality rate of the air test are greater than 20%, the entire test must be repeated. C5 calculation
Calculate the three repeated test data according to the machine value method to calculate KT50.99
iiKAoNrKAca
GB 18416--2001
GB18416-2001 "Household sanitary insecticide disc mosquito coils" and other 4 national standards No. 1 amendment
This amendment has been approved by the State Administration of Standardization on February 10, 2003 in the document No. 9 of the National Standardization Administration of Agricultural and Light Industry [2003], and will be implemented from the date of publication.
GB18416—2001 Household Hygiene Insecticide Coil" Clause No.
Table 2 Efficacy
Table 2 Thermal Stability
4.1, 4.3, 4.74.8; 1.9 of this standard are mandatory, and the rest are recommended. KT50≤7min
The degradation rate of the active ingredient content of pyrethroids
5%, the degradation rate of the active ingredient content of other types is 10%.
**., KT50>7min is judged as unqualified,
, pyrethroids>5 is judged as unqualified
Other types>10 are judged as unqualified.
Modified to
4.1, 4.3, 4.7, 4.8, 4.9, 7.1 of this standard. 1. 7.4 and 7.5 are mandatory, and the rest are recommended. KT508.0 min.
, the degradation rate of the active ingredient content of pyrethroids is ≤10%, and the degradation rate of the active ingredient content of other types is more than 15%.
..-, KT50>8.0min is judged as unqualified. , Pyrethroids>10% is judged as unqualified
Other types>15% are judged as unqualified.6 Balance, graduation value is 0.1mg.
B2.1.7 Crusher.
B2.1:8 Sieve: standard test sieve 0.154 mm (100 day/inch). B2.1.9 Filter paper.
B2.1.10 Oscillator,
B2.2 Reagents
B2.2.1 Internal standard: tributyl phthalate, dibutyl phthalate, without interfering impurities (the applicable scope of internal standard is solid; dibutyl phthalate is applicable to samples without octachlorodipropyl ether (S), and monoamyl phthalate is applicable to samples with octachlorodipropyl ether). B2.2.2 Solvent: acetone, methanol (analytical grade), B2.2.3 Standard products: allethrin, ethrin, etc., B2.3 Gas chromatography conditions
B2.3.1 Temperature
B2.3. 1. 1 Column temperature: 190C.
B2. 3.1.2 Vaporization temperature: 210℃ or 250℃. B2.3.1.3 Detector temperature: 210℃ or 250℃. B2.3.2 Gas flow rate (kg/cm2).
B2.3.2.1 Carrier gas: Nitrogen 1.0.
R2.3.2.2 Fuel gas: Hydrogen 0.。
B2.3.2.3 Fuel-supporting gas: 0.5% air.
The above operating conditions are typical operating parameters. According to the characteristics of different instruments, the given operating conditions can be appropriately adjusted to obtain the best effect.
B3 Preparation of standard solution
In a 15 mL weighing bottle, accurately weigh about 0.03 g ~ 0.04 g (accurate to 0.0002 g) of the standard sample equivalent to the sample to be tested, and then weigh 0.015g ~ 0.025g (accurate to 0.0002g) of dibutyl phthalate or diamyl phthalate, add 5ml of acetone solution, and after fully dissolving, close the shaker and put it in the refrigerator for use. B4 Preparation of sample solution
B4.1 Sample pretreatment
Crush the finished mosquito coil with a grinder and pass it through a 0.151 m (100 mesh/inch) standard test sieve. The mosquito coil powder is ready for use. B4.2 Preparation of mosquito coil extract
Accurately weigh 10 g to 15 g of mosquito coil powder in a 100 mL stoppered conical flask (accurate to 0.000 2 g: the estimated content of the measured component is about 0.03 g to 0.04 g). Then weigh 0.015 g to 0.025 g of internal standard (accurate to 0.0002 g). Finally, add methanol-lactone mixed solution (volume ratio 1:1) with a slightly excessive amount to cover the sample. Vibrate the mixture on an oscillator for 20 minutes and filter it until it is 10 mL glass bottle for gas chromatography analysis,
-TKAONiKAca-
B5 determination
GB184162001
Under the chromatographic conditions in 12.3, after the instrument is stable, continuously inject the standard solution and sample solution with a micro syringe to calculate the peak area ratio of the sample (standard sample) to the internal standard in the chromatogram of each needle. When the peak area ratio of the two adjacent needles is basically parallel and stable, perform gas chromatography analysis according to the above conditions.
B6 Calculation
Average the measured sample peak to internal standard peak area ratios in the two sample solutions and the internal standard peak to standard peak area ratios in the standard solution, and calculate the content Mx correction factor of the component to be measured according to formula (BI) and formula (B2):
Amount of component to be measured:
Where: A.
f -A,Xmia/mzXCt
Mx(%)=fXm/m1XA.X10)
The average value of the sample peak to internal standard peak area ratio in the sample solution; The half-mean of the internal standard peak to standard peak area ratio in the standard solution: The mass of the internal standard in the sample solution, the mass of the internal standard in the standard solution;
The mass of the sample in the sample solution:
The mass of the standard substance in the standard solution, the content of each standard substance, %.
Appendix C
(Standard Appendix)
Method for indoor efficacy determination of mosquito coils
For testers: Use standard test mosquitoes; the female adult mosquitoes that are the second to third largest to suck blood after emergence, Culex pipiens pallens is used in northern areas, and Culex pipiens quinquefasciatus is used in southern areas. C2 Test conditions
Room temperature: (26±2)
Relative humidity: (60±5)%
C3 Instruments
(B2)
C3. 1 Closed cylinder device (see Figure C1): A glass or transparent colorless plastic cylinder (C) with an inner diameter of 20 rm and a height of 43 cm is placed on this frame (I). There are 27 cm diameter glass or transparent colorless round plates (E, F) on the upper and lower sides of the side cylinder. There is a 2 cm diameter round hole in the center of the upper round plate, which is plugged with a rubber plug (G). There is a 5 cm diameter round hole in the center of the lower round plate, which is plugged with a rubber plug (H). There is a mosquito coil rack (B) on the rubber plug (H). It is used to test the number of incense. The joints between the cylinder and the upper and lower round plates are respectively padded with rubber pads (ID,,D2) to prevent smoke leakage. 698
GB18416—2001
Figure C1 Closed cylinder device
A mosquito coil; P--mosquito coil holder: C--glass or transparent colorless plastic plate, commercial 43 cm. Inner weight 20 cm11tD1.D, rubber gasket; E--glass or transparent colorless plastic circular plate, 27 cm, with a hole of 2 cm in diameter in the center; F--glass or transparent colorless plastic circular plate, 27 cm in diameter, with a hole of 5 cm in diameter in the center; G, H--rubber stopper; I--wooden frame, 30 cm high C3.2 mosquito suction tube,
C3.3 stopwatch.
C3.4 counter.
C4 Test Steps
Use a straw to suck 20 test mosquitoes from the cage, release them from the central circular hole of the circular plate (F) below the closed cylinder, plug the rubber plug (H) and wait for the test mosquitoes to come out and resume normal activities. Take any section of the mosquito coil to be tested, place it horizontally on the mosquito coil rack, light the mosquito coil and start the timer. Smoke for 1 minute. Then remove the mosquito coil and immediately plug the rubber plug (H). Record the number of test mosquitoes knocked down at regular intervals. The test is repeated at least three times. After each test, the entire test device must be cleaned before the next test. At the same time, an air test must be carried out. If the knockdown rate and mortality rate of the air test are greater than 20%, the entire test must be repeated. C5 calculation
Calculate the three repeated test data according to the machine value method to calculate KT50.99
iiKAoNrKAcabzxZ.net
GB 18416--2001
GB18416-2001 "Household sanitary insecticide disc mosquito coils" and other 4 national standards No. 1 amendment
This amendment has been approved by the State Administration of Standardization on February 10, 2003 in the document No. 9 of the National Standardization Administration of Agricultural and Light Industry [2003], and will be implemented from the date of publication.
GB18416—2001 Household Hygiene Insecticide Coil" Clause No.
Table 2 Efficacy
Table 2 Thermal Stability
4.1, 4.3, 4.74.8; 1.9 of this standard are mandatory, and the rest are recommended. KT50≤7min
The degradation rate of the active ingredient content of pyrethroids
5%, the degradation rate of the active ingredient content of other types is 10%.
**., KT50>7min is judged as unqualified,
, pyrethroids>5 is judged as unqualified
Other types>10 are judged as unqualified.
Modified to
4.1, 4.3, 4.7, 4.8, 4.9, 7.1 of this standard. 1. 7.4 and 7.5 are mandatory, and the rest are recommended. KT508.0 min.
, the degradation rate of the active ingredient content of pyrethroids is ≤10%, and the degradation rate of the active ingredient content of other types is more than 15%.
..-, KT50>8.0min is judged as unqualified. , Pyrethroids>10% is judged as unqualified
Other types>15% are judged as unqualified.3 Standard products: allethrin, endothrin, etc. B2.3 Gas chromatography conditions
B2.3.1 Temperature
B2.3. 1. 1 Column temperature: 190°C.
B2. 3.1.2 Vaporization temperature: 210°C or 250°C. B2.3.1.3 Detector temperature: 210°C or 250°C. B2.3.2 Gas flow rate (kg/cm2).
B2.3.2.1 Carrier gas: nitrogen 1.0.
R2.3.2.2 Fuel gas: hydrogen 0..
B2.3.2.3 Fuel gas: air 0.5.
The above operating conditions are typical operating parameters. According to the characteristics of different instruments, the given operating conditions can be appropriately adjusted to obtain the best effect.
Preparation of B3 standard solution
In a 15 mL weighing bottle, accurately weigh about 0.03 g ~ 0.04 g (accurate to 0.0002 g) of the standard sample equivalent to the sample to be tested, and then weigh 0.015g ~ 0.025g (accurate to 0.0002g) of dibutyl phthalate or diamyl phthalate, add 5ml of acetone solution, after fully dissolved, close the shaker and put it in the refrigerator for use. B4 Preparation of sample solution
B4.1 Sample pretreatment
Crush the finished mosquito coil with a grinder and pass it through a 0.151 m (100 mesh/inch) standard test sieve. The mosquito coil powder is ready for use. B4.2 Preparation of mosquito coil extract
Accurately weigh 10 g to 15 g of mosquito coil powder in a 100 mL stoppered conical flask (accurate to 0.000 2 g: the estimated content of the measured component is about 0.03 g to 0.04 g). Then weigh 0.015 g to 0.025 g of internal standard (accurate to 0.0002 g). Finally, add methanol-lactone mixed solution (volume ratio 1:1) with a slightly excessive amount to cover the sample. Vibrate the mixture on an oscillator for 20 minutes and filter it until it is 10 mL glass bottle for gas chromatography analysis,
-TKAONiKAca-
B5 determination
GB184162001
Under the chromatographic conditions in 12.3, after the instrument is stable, continuously inject the standard solution and sample solution with a micro syringe to calculate the peak area ratio of the sample (standard sample) to the internal standard in the chromatogram of each needle. When the peak area ratio of the two adjacent needles is basically parallel and stable, perform gas chromatography analysis according to the above conditions.
B6 Calculation
Average the measured sample peak to internal standard peak area ratios in the two sample solutions and the internal standard peak to standard peak area ratios in the standard solution, and calculate the content Mx correction factor of the component to be measured according to formula (BI) and formula (B2):
Amount of component to be measured:
Where: A.
f -A,Xmia/mzXCt
Mx(%)=fXm/m1XA.X10)
The average value of the sample peak to internal standard peak area ratio in the sample solution; The half-mean of the internal standard peak to standard peak area ratio in the standard solution: The mass of the internal standard in the sample solution, the mass of the internal standard in the standard solution;
The mass of the sample in the sample solution:
The mass of the standard substance in the standard solution, the content of each standard substance, %.
Appendix C
(Standard Appendix)
Method for indoor efficacy determination of mosquito coils
For testers: Use standard test mosquitoes; the female adult mosquitoes that are the second to third largest to suck blood after emergence, Culex pipiens pallens is used in northern areas, and Culex pipiens quinquefasciatus is used in southern areas. C2 Test conditions
Room temperature: (26±2)
Relative humidity: (60±5)%
C3 Instruments
(B2)
C3. 1 Closed cylinder device (see Figure C1): A glass or transparent colorless plastic cylinder (C) with an inner diameter of 20 rm and a height of 43 cm is placed on this frame (I). There are 27 cm diameter glass or transparent colorless round plates (E, F) on the upper and lower sides of the side cylinder. There is a 2 cm diameter round hole in the center of the upper round plate, which is plugged with a rubber plug (G). There is a 5 cm diameter round hole in the center of the lower round plate, which is plugged with a rubber plug (H). There is a mosquito coil rack (B) on the rubber plug (H). It is used to test the number of incense. The joints between the cylinder and the upper and lower round plates are respectively padded with rubber pads (ID,,D2) to prevent smoke leakage. 698
GB18416—2001
Figure C1 Closed cylinder device
A mosquito coil; P--mosquito coil holder: C--glass or transparent colorless plastic plate, commercial 43 cm. Inner weight 20 cm11tD1.D, rubber gasket; E--glass or transparent colorless plastic circular plate, 27 cm, with a hole of 2 cm in diameter in the center; F--glass or transparent colorless plastic circular plate, 27 cm in diameter, with a hole of 5 cm in diameter in the center; G, H--rubber stopper; I--wooden frame, 30 cm high C3.2 mosquito suction tube,
C3.3 stopwatch.
C3.4 counter.
C4 Test Steps
Use a straw to suck 20 test mosquitoes from the cage, release them from the central circular hole of the circular plate (F) below the closed cylinder, plug the rubber plug (H) and wait for the test mosquitoes to come out and resume normal activities. Take any section of the mosquito coil to be tested, place it horizontally on the mosquito coil rack, light the mosquito coil and start the timer. Smoke for 1 minute. Then remove the mosquito coil and immediately plug the rubber plug (H). Record the number of test mosquitoes knocked down at regular intervals. The test is repeated at least three times. After each test,
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