This standard specifies the procedures and methods for monitoring the epidemic situation of tulip bulbs during the planting period. This standard is applicable to the epidemic situation monitoring of tulip bulbs imported from abroad. GB/T 23625-2009 Tulip bulb epidemic situation monitoring procedures GB/T23625-2009 Standard download decompression password: www.bzxz.net
This standard specifies the procedures and methods for monitoring the epidemic situation of tulip bulbs during the planting period. This standard is applicable to the epidemic situation monitoring of tulip bulbs imported from abroad.
Appendices A, B and C of this standard are informative appendices.
This standard is proposed and managed by the National Technical Committee for Plant Quarantine Standardization. The
drafting units of this standard: National Agricultural Technology Extension Service Center, Beijing Plant Protection Station, China Agricultural University, China Institute of Inspection and Quarantine.
The main drafters of this standard: Wang Yuxi, Zheng Jianqiu, Li Zhihong, Zhao Jiwen, Ding Jianyun, Zhang Jianhua, Shao Gang.
The clauses in the following documents become the clauses of this standard through reference in this standard. For all dated referenced documents, all subsequent amendments (excluding errata) or revisions are not applicable to this standard. However, parties that reach an agreement based on this standard are encouraged to study whether the latest versions of these documents can be used. For all undated referenced documents, the latest versions apply to this standard.
Glossary of Phytosanitary Terms ISPM5

ICS 65, 020. 01
National Standard of the People's Republic of China
GB/T23625--2009
Guidelines for quarantine surveillance on imported tulips2009-04-27Issued
General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of ChinaChina National Standardization Administration
2009-10-01Buy Application
Appendices A, B and C of this standard are informative appendices. This standard is proposed and managed by the National Technical Committee on Plant Quarantine Standardization. GB/T23625—2009
Drafting organizations of this standard: National Agricultural Technology Extension Service Center, Beijing Plant Protection Station, China Agricultural University, China Institute of Inspection and Quarantine.
Drafters of this standard: Shi Tianxi, Zheng Jianqiu, Li Zhihong, Zhao Jiwen, I Jianyun, Zhang Jianhua, Shao Gang. 1
1 Scope
Tulip bulb epidemic monitoring procedures
This standard specifies the procedures and methods for epidemic monitoring of tulip bulbs during the planting period. This standard applies to epidemic monitoring of tulip bulbs introduced from abroad. 2 Normative references
GB/T23625—2009
The clauses in the following documents become clauses of this standard through reference in this standard. For any dated referenced document, all subsequent amendments (excluding errata) or revisions are not applicable to this standard. However, parties to an agreement based on this standard are encouraged to study whether the latest versions of these documents can be used. For any undated referenced document, the latest versions shall apply to this standard. Plant quarantine terminology discussion table ISPM5
3 Terms and definitions
Terms and definitions established in the plant quarantine terminology vocabulary ISPM5 apply to this standard. 4 Pests to be monitored
Pests related to tulip bulbs in the List of Quarantine Pests for Plants Entering the People's Republic of China (Announced by the Ministry of Agriculture on May 29, 2007) and the List of National Plant Quarantine Pests (Announced by the Ministry of Agriculture on March 2, 2006), with a focus on monitoring the quarantine pests proposed in the Quarantine Approval Form for Introduced Seedlings and Seedlings. 5 Monitoring equipment, tools and supplies
5.1 Pest monitoring
Equipment and tools: black light, yellow board, blue board, insect net, brush, finger-shaped tube, poison bottle, three-removal paper bag, handheld magnifying glass, microscope, dissecting mirror, insect dissecting needle, etc.
Supplies: 95% ethanol, xylene, ether, distilled water, etc. 5.2 Nematode monitoring
Equipment and tools: dissecting microscope, optical microscope, funnel, shallow sieve plate, mesh screen (20 mesh, 400 days), test tube, culture dish, small mixer, concave glass slide, flat glass slide, glass fiber, alcohol lamp, constant temperature water bath, electric heating plate, barge pipe, plate, lower burner, puncher, shovel, pruning shears, knife, polyethylene plastic bag, oil pen, etc.
Supplies: glycerol, 95% ethanol, steam filling water, phenol, lactophenol, neutral gum, paraffin, silica gel, calcium chloride (CaCl,), etc. 5.3 Disease monitoring
Equipment and tools: microscope, glass slide, various specifications of test tubes and culture blood, triangular flask, grinder, marker, pipette, alcohol lamp, sterile operation workbench, constant temperature and moisturizing incubator, refrigerator, balance, scissors, small shovel, paper bag or plastic bag, handheld magnifying glass, etc. Supplies: 95% ethanol, distilled water, neutral gum, etc. 6 Epidemic monitoring
6. 1 Monitoring period
Tulip growth period.
6.2 Monitoring methods
According to the quarantine approval opinion, if two-separation trial planting is required, in the place designated by the plant quarantine agency with isolation conditions, according to the biological characteristics of the pests monitored in key 1
GB/T 23625-2009
and the growth period of tulips, 2 to 3 surveys shall be conducted during the isolation period, and attention shall be paid to maintaining a reasonable observation time interval, and samples shall be taken for laboratory testing in time if symptoms are found; in the introduction of seeds and seedlings quarantine approval form, if centralized planting or unlimited planting conditions are required, or centralized planting of tulip bulbs for production is required, 1 or 2 inspections shall be conducted during the tulip growing season, and samples shall be taken for laboratory testing in time if symptoms are found. If there are no obvious symptoms, the survey is conducted by selecting points at equal intervals, with 10 plants at each point (for areas below 667m2, select 5 sample points; for areas between 667m2 and 2×667m2, select 10 sample points; for areas above 4×667m2, select 20 sample points; for areas above 4×667m2, select 30 sample points). Fill in the Epidemic Monitoring Survey Form (see Appendix A), find symptoms, and collect samples. 7 Laboratory Identification
Observe the morphology of the pests, isolate and culture the pathogens, and make identification for the suspicious samples found in the monitoring. 8 Sample Processing
8.1 Pest Samples
Small tailed worms, eggs, spinning worms, and pupae are preserved by soaking in 75% ethanol; butterfly and moth worms are preserved in triangular paper packages with good water absorption; large worms with longer bodies are stored by rolling the paper into a leaning shape. Label well and keep well. 8.2 Pathogenic samples
Disease plants and diseased tissues should be sealed in appropriate glossy paper or plastic bags, and labels should be affixed inside and outside the envelopes. Fungal and bacterial disease specimens should be placed in a 4-degree refrigerator for freshness preservation. Virus specimens should be placed in a -20°C low-temperature refrigerator for preservation. 8.3 Nematode samples
Disease plants and diseased tissues should be placed in polyethylene plastic bags. If the samples cannot be separated in time, they can be stored in a room at 20°C to 25°C. Open the bag for ventilation, and pay attention to moisture retention and avoid sunlight. 8.4 Living samples
Living samples should be stored for no more than one month. After the test, except for micro-specimens, the rest should be inactivated. 9 Epidemic identification report
After the laboratory identification is completed, fill in the plant pest sample identification report (see Appendix B). 10 Epidemic Monitoring Report
Record the monitoring results and fill in the "Epidemic Monitoring Record Form". The plant quarantine agency will compile and summarize the monitoring results to form the "Plant Epidemic Monitoring Report (see document C)" and report it step by step as required. 11 File Management
Record and summarize the results of the survey in the monitoring area in detail. The original records of various monitoring and other materials should be well preserved in the plant quarantine agency. The collected samples that are identified as epidemics and have preservation value should be made into specimens, filled in with specimen labels, and preserved in the quarantine agency. 2
Monitored plants
Monitoring location
Symptom description:
Name of organism
Appendix A
(Information appendix)
Epidemic monitoring questionnaire
Table A1 Epidemic monitoring questionnaire
Monitoring unit
Contact number
Occurrence
Monitoring unit (seal) 1
Monitor (signature)
GB/T 23625—2009
GB/T 23625--2009
Plant name
Plant growth period
Source of samples
Submitting unit
Testing and identification method:
Testing and identification result:
Note:
Supervisor (signature):
Reviewer (signature):
Appendix B
(Informative appendix)
Plant pest sample identification report
B.1 Plant pest sample identification report Variety name
Number of samples
Inspection month
Note: This herbal medicine is in triplicate, with one copy for each of the testing unit, inspected unit and quarantine agency. Sample part
Submitter
Contact number
Identification unit (special seal):bzxz.net
No.:
Plant name:
Country (region) of origin of the planting shade:
Planting place:
List of pests to be inspected:
(Chinese name and scientific name)
Monitoring and inspection methods:
Epidemic monitoring results;
Quarantine unit (Special seal for plant quarantine:
(Informative Appendix
Plant epidemic monitoring report
Plant epidemic monitoring report
Variety name:
Introduction number of trays
Planting area:
Planting date:
GB/T 23625—2009
Full-time quarantine officer (signature):
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