This standard gives the main morphological and structural characteristics, growth and reproduction, genetic characteristics, and detection methods of bighead carp (Aristichthys nobilis). This standard is applicable to the identification of bighead carp germplasm. GB 17718-1999 Bighead carp GB17718-1999 Standard download decompression password: www.bzxz.net

GB17718—1999
This standard is formulated in order to identify, protect and preserve the excellent traits and germplasm of original seeds, avoid the mixing and degradation of germplasm during seedling production, and carry out effective monitoring of purchased germplasm. This standard is mainly based on the scientific and technological research results of the Eighth Five-Year Plan, and also draws on the relevant results of the scientific and technological research results of the Sixth Five-Year Plan and the Seventh Five-Year Plan. Appendix A of this standard is a suggestive appendix.
This standard is proposed by the Ministry of Agriculture of the People's Republic of China. This standard is under the jurisdiction of the Freshwater Aquaculture Sub-Technical Committee of the National Aquatic Products Standardization Technical Committee. This standard was drafted by: Shanghai Fisheries University, Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences. The main drafters of this standard are Li Sifa, Zhao Jinliang, Cai Wanqi, Zhou Biyun, Lü Guoqing, Fan Zhaoting and Xu Zhongfa. This standard shall come into effect on July 1, 1999. 97
1 Scope
National Standard of the People's Republic of China
Bighead carp
GB 17718—1999
This standard gives the main morphological and structural characteristics, growth and reproduction, genetic characteristics, and detection methods of (Aristichthys nobilis).
This standard applies to the germplasm identification of bighead carp.
2 Cited standards
The provisions contained in the following standards constitute the provisions of this standard through reference in this standard. When this standard is released, the versions shown are valid. All standards are subject to revision and parties using this standard should investigate the possibility of using the most recent versions of the following standards. GB17716-—1999 Black carp
3 Name and classification
3.1 Scientific name
(Aristichthys nobilis).
3.2 Classification position
Belongs to the order Cypriniformes, the family Cprinidae, the subfamily Hypophthalmichthyinae, the genus Aris-tichthys.
4 Main morphological and structural characteristics
4.1 External morphological characteristics
4.1.1 Appearance
The body is laterally flattened and slightly high, with the ventral ridge extending from the base of the pelvic fin to the anus. The head is enlarged, about one-third of the body length, with a wide mouth, a blunt snout, and small eyes located below the mid-axis of the head. Scales small, lateral line complete. The gills are closely arranged but not fused, and have suprabranchial apparatus. The caudal fin is deeply forked. Tip of pectoral fins extending well beyond base of pelvic fins.
The head and back are gray-black with some light yellow, and there are black spots scattered on both sides of the body. The abdomen is silvery white and the fins are gray-black. The external morphology of bighead carp is shown in Figure 1.
Approved by the State Administration of Quality and Technical Supervision on April 5, 1999, 98
Implemented on July 1, 1999
4.1.2 Countable traits
4.1.2.1 Dorsal fin type D3.7~8.
4.1.2.2 Anal fin type: A3.11~15.
GB 17718--1999
Figure Bighead carp appearance
4.1.2.3 Lateral line scale type: 83~11
22～27
4.1.2.4 Number of gill spurs outside the first gill arch: 217～293. 4.1.3 Measurable traits
For individuals with a body length of 28.1~~110.9cm and a body weight of 500.0~~23500.0g, the proportion values ​​of the measured traits are shown in Table 1. Table 1 Ratio of measured measurable traits
Total length/body length
Body length/body height
4.2 Internal structural characteristics
Body length/head lengthbzxz.net
Head length/snout length
Two chambers, the anterior chamber is long and inflated, the posterior chamber is small and conical. 4.2.2 Pharyngeal teeth
Flat, smooth tooth surface. Gear type 4/4.
4.2.3 Vertebrae
Total number of vertebrae: 39~41.
4.2.4 Peritoneum
Dark black.
5 Growth and Reproduction
5.1 Growth
Head length/eye diameter
Head length/eye distance
Body length/tail peduncle length
Caudal peduncle length/tail peduncle height
The measured values ​​of body length and weight of fish in different age groups are shown in Table 2. The average decalculated values ​​of body length and weight, growth equation, and body length-weight relationship of fish of different age groups corresponding to Table 2 are shown in Appendix A (suggestive appendix). 99
Age\, age
Measured body length
Measured weight
Measured body length
Measured weight
GB 17718—1999
Measured values ​​of body length and weight of fish in different age groups Table 2
1) Age is mainly identified based on the number of annual rings on scales. Reproduction
2700～
2 700～
5.2.1 Age of sexual maturity: females 4 to 6 years old, males 3 to 5 years old. 5.2.2 The gonads of sexually mature individuals mature once a year and spawn once a year. Number of bad eggs: The number of eggs of individuals in different age groups is shown in Table 3. 5.2.3
5500～
9000～
8250～
Table 3 Number of eggs of individuals in different age groups
Age, age
Weight name
Absolute number of eggs, grains
Relative number of eggs, grains/g
6 Genetic characteristics
6.1 Cytogenetic characteristics
6.1.1 Chromosome number
8 500. 0±353. 0
(4. 14±1. 30) ×105
48.3±13.8
Somatic cell chromosome 2 times, 2n=48.
6.1.2 Karyotype
11 564. 3±682. 3
(7.50±3.82)×105
55.9±33.2
10 600~
12 428. 6±1 273. 7
(7.63±4.47)×105
61.1±34.8
11750~
11200~
15600~
15 975. 0±306. 2
(9.65±2.07)×105
60. 4±12.8
12 pairs of mid-centromere chromosomes (m group); 9 pairs of sub-metacentric chromosomes (sm group); 3 pairs of sub-telomeric chromosomes (st group), number of chromosome arms (NF) 90 (see Figure 2). AM
2 Biochemical genetic characteristics
Chromosome karyotype of bighead carp
6.2.1 Lactate dehydrogenase (LDH) isozymes in muscle GB 17718—1999
6.2.1.1 The electrophoresis zymogram of lactate dehydrogenase (LDH) isozymes in muscle is shown in Figure 3. 6.2.1.2 The enzyme band scanning diagram of lactate dehydrogenase (LDH) isozymes in muscle is shown in Figure 4. 0
Figure 3 Electrophoresis zymogram of LDH isozymes in bighead carp muscle LDH,
Figure 4 Enzyme band scanning diagram of LDH isozymes in muscle
6.2.1.3
The activity intensity of lactate dehydrogenase (LDH) isozyme bands in muscle is shown in Table 4. Table 4 Activity intensity of muscle LDH enzyme band
Activity intensity
4 Population variation range: The proportion of polymorphic loci is 15.8%~29.4%, and the average heterozygosity is 0.0772~0.1133. 6.2. 1.4
Detection method
7.1 Identification of age
Perform in accordance with the provisions of GB17716.
7.2 Determination of fertility
The determination shall be carried out in accordance with the provisions of GB17716.
7.3 Chromosome detection
Perform in accordance with the provisions of GB17716.
7.4 The zymogram of lactate dehydrogenase (LDH) isozymes shall be in accordance with the provisions of GB17716.
GB 17718-
—1999
Appendix A
(Suggested Appendix)
Calculated values ​​of body length and weight at different age groups, growth equation, body length and weight relationship A1Calculated values ​​of body length and weight
The average calculated values ​​of body length and weight of fish of different age groups corresponding to Table 2 are shown in Table A1Table A1
Age, age
Calculated body length, cm
Calculated weight name
Calculated body length.cm
Calculated weight·g
Growth equation
Female fish:
Male fish:
Where: L.-
Average calculated values ​​of body length and weight of fish of different age groups1+
L, -- 136.0[1 --e
0. 152 1(1 0. 010 4) -
W,=47 424. 3[1-e-0. 152 1(-0. 010 4) 73L,=100. 1[1— - 0. 274 5( 0. 321 9)W,=18 306. 2[1-e-0. 274 5±-0. 321 9)]-body length of fish at t age, cm;
-body weight of fish at t age, g,
natural logarithm;
age of fish.
Body length and weight relationship formula
Fish stage
W 0. 035 2 X L2. 7442
Where: W
Fish body weight, g;
—fish body length, cm.
Edible commercial fish stage
W 0. 017 0 X L3 034 2
Broodstock stage
Female fish:
Male fish:
W = 0. 063 1 X L2. 750 8
W = 2. 091 0 X L1. 9399
（A1）
（A2）
（A3）
（A4）
（A5）
·（A6)
（A7)
（A8)
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