Some standard content:
Agricultural Industry Standard of the People's Republic of China
NY 318-1997
Ginseng products
Ginseng products
1997-08-27 Issued
The Ministry of Agriculture of the People's Republic of China
1998-03-01 Implementation
N318—1997
Ginseng products mainly include various brands of ginseng tea (Yijiao ginseng tea, Yiquan ginseng tea, Changbaishan ginseng tea, Changxian ginseng tea, Huangjun tea, Korean ginseng tea, Lihong tea, Fenggeng ginseng tea, etc.), ginseng fruit tea, Qiuji ginseng fruit tea, Rhodiola rosea ginseng tea, ginseng honey tablets (Xie ginseng honey tablets made of fresh ginseng dream tablets, ginseng fruit lung, ginseng drink, etc.) and ginseng wine, etc. These products are all guaranteed products. Except for the total ginseng, red ginseng and total ginseng, which are newly formulated methods, the rest are in accordance with national standards The specified method is used for inspection. Physical and chemical indicators, bio-indicators, after actual inspection of various samples received, various indicators are determined according to the results. It is stipulated that the samples must be used in a short period of time, so the limit is determined under the signature item: This standard is for the market
This standard is proposed by the Ministry of Agriculture of the People's Republic of China, and this standard is jointly developed by Jilin University. The main contributors to the standard: Li Shuning, Liu Tuofeng, Rang Ju, Wang Yanhui, Qi Rui, Li Yang. 1 Scope
Agricultural Industry Standard of the People's Republic of China
Ginseng Products
Ginkeng pradug.ig
NY3181997
This standard specifies ginseng tea, mixed fruit, multi-dimensional longevity fruit tea, red ginseng tea, filial piety! , filial piety wine technical requirements, anti-standard methods, inspection rules, environmental table, packaging, transportation, storage in this standard for dry ginseng tea, ginseng fruit, multi-dimensional fruit tea, Kaiju Tian ginseng steamed, honey slices, wine: 2 Reference standards
The following standards contain provisions, through reference in this standard and constitute For the provisions of the standard, the versions shown are effective when this standard is issued. All standards will be revised, and the parties using this standard should explore the possibility of using the latest version of the following standards. GR191--1999 Pictorial signs for packaging and transportation
175.2-1999 Food hygiene microbiological inspection diagram GB176S.319 Food microbiological inspection of bacteria G34789.5-1994 Food hygiene and freshness inspection of cattle GB/T 6000.2—:085
Determination of moisture in foods
G/7 5009.4--1585
Determination of sodium ions in foods
GB/T 009.R-19A5
Determination of sodium ions in foods
GBT 5009.111996
CB/5009.12—1996
Determination of lead in foods by reduction method
GB/T 5009. S-.-1596
Determination method of hexavalent iodine in food
GB/5009.10—1996 Determination method of hexavalent iodine residues in food GB77181551 General standard for food labeling
GB/151044-1959 Labeling of alcoholic beverages
GB/1U45-1—1939 Method for testing alcoholic beverages G/146 Standard for the inspection of white liquor
GR/T1SG—990 Determination method of riboflavin (raw 1) in food by two-dimensional method
GB/T12397--.1999
GH123921990 Determination of total ascorbic acid in vegetables, fruits and their products by fluorescence method and 21-diyl violet method3 Definition
This standard adopts the following standards.
3.1 Human miscellaneous
Human rich, fungus grapes, with other materials such as refined powder. 3.2 Human tea
Ministry of Agriculture of the People's Republic of China approved on 1997--27, 1998 0301 implementation
NY318-1997
Ginseng fruit paste, with other materials scientifically processed and refined powder. 3.3 Multi-dimensional longevity fruit tea
Ginseng juice paste, with ginseng, with sugar, with other auxiliary materials scientifically processed and refined powder. 3.4 Red ginseng tea
Ginseng, alpine red, Dajing, green, island fence, etc., with other auxiliary materials scientifically processed and refined powder. 3.5 Ginseng slices
Fresh ginseng is sliced or cut into pieces, cooked and baked in honey eggs. 3.6 Seasonal liquor
Liquor made from fresh ginseng, white wine, edible color and other raw materials. 4 Technical requirements
4.1 Quality and quality indicators
Human flower
Brown or brown
Human fruit tea
Explore its blood or brown
Zhongxiong zero tea
Free fast
! Put it aside, only you can thank you, with the original heat state of ginseng, the temperature is 0.01-0.555 μm--1.085 μm, the temperature is 0.15-0.500 μm, the temperature is 0.25-0.800 μm, the temperature is 0.35-0.800 μm, the temperature is 0.50-0.800 μm, the temperature is 0.25-0.800 μm, the temperature is 0.35-0.800 μm, the temperature is 0.35-0.800 μm, the temperature is 0.35-0.800 μm, the temperature is 0.35-0.800 μm, the temperature is 0.35-0.800 μm, the temperature is 0.35-0.800 μm, the temperature is 0.35-0.800 μm, the temperature is 0.35-0.800 μm, the temperature is 0.35-0.800 μm, the temperature is 0.35-0.800 μm, the temperature is 0.35-0.800 μm g±.15g
Rhodiola rosea people often chrysanthemum
brown production, or alert color
effective solution, envoy, number
, H people participate in high
on red rose big special letter
color makeup, prosperous
R00μm--05c 1m
effective people roast water letter market
comfortable, flow business early
constant color, base wood mixed lotus
\g±c.15g
people age lack piece
brown to red compensation color
F has seven flavors
people seek unique ones
qi and retention
'pre-slice, piece record
learn whole, light band, no
word piece and miscellaneous!
Cut into small pieces, no
filial piety wine
nourishing yellow, brown or even
charcoal color
, it has the unique smell of white wine and
people
The wine contains
: no promotion, no suspended matter
500 ml.±; ml.
- cx ml.±$ml.
1?5f rnJ.=10 rn].
1 Epe unz u..
1 75 m. nt.
Box 15~30g
4.2 Physical and chemical indicators
Physical and chemical indicators are shown in Table 2.
Ren'an Zong Dingcheng
Hongjingquanshi
Lushengjia Ht
Huaishengban
Weisheng rate heart
Zongling "According to Yirong"
Medical shape before
(According to Yishijiang and Yidingxing)
666
Five-stimulant forbidden
According to Fb)
Lithium (According to)
Wei biological indicators
8/:00 taL
g/1u0ml
mng/kg
Jiuy/hy
Rendong tea
NY 318—1997
Table 2 Physical and chemical standards
Human fruit tea Vitamin fruit tea Rhodiola rosea mixed 3 +0. 2
$:2- c
.0. 05
Human deficiency tablets
5±0.2t10±c.3
Ginseng tea, ginseng fruit tea, multivitamin whole fruit tea, Rhodiola rosea tea, ginseng honey tablets, ginseng honey microbial status cup Table 3. Table 3 Biological indicators
Coliform group
Scald disease
Spring tea, multi-fruit, male ginseng fruit, benzoic red, secret element, love tablets 1no
Original holding joy
Not detectable
Table wine
People receiving code
Not detectable
5 sampling
NY 318—1997
5.1 Before sampling, the product name, origin, specification, grade and packaging standard should be noted: check the completeness of the packaging, cleanliness and pollution, etc., and record them in detail. Those with problems should be sampled separately for detailed inspection. 5.2 Sampling Quantity Decision
If the quantity of the same batch number product is less than 1 piece, sample 5 pieces and then take them from the box: 1 piece~1 piece, take samples according to %, and then randomly select one piece from each piece: if the quantity exceeds 1 piece, the excess part will be sampled according to 1 piece, and if the quantity is less than 5 pieces, sample 1 piece. Different batches of small products should follow the above principle, sample from the original batch, and keep it as a secondary sample for one year. 6 Test method
6.1 Sample preparation
Ginseng tea should be tasted first, and then it can be directly measured and packed with ginseng slices. Place the slices in a porcelain plate, dry at 7℃, and then pass through a No. 1 sieve. After drying, use a suction pipette to measure the powder. Microbiological test: follow the detailed microbiological test method. 6.2 Test according to the specification
6.2.1 Color
Take ginseng slices, ginseng fruit, multivitamin fruit tea, red ginseng adult guest killing bags, and evenly spread them on concave paper to observe whether the color of the grains is uniform or whether there are other foreign objects. If any abnormality is found: re-sample and test. Take 5 bags of ginseng slices, spread them all in a white plate, check the color of the ginseng segments, and there should be no flower pieces or pits. Take the ginseng and put them in a blue 2CCm flask to observe its color. 6.2.7 Take 5 bags of ginseng tea, ginseng fruit, multi-dimensional ginseng black package rhodiola rosea ginseng tea, put them into the sieve, keep the sieve horizontal, and gently sieve back and forth. Each sieve number is sieved 3 times. The total amount of the residue that cannot pass through the sieve and the residue that can pass through the round sieve should not exceed 8.0. Take 5 bags of multi-dimensional slices, spread them evenly on a porcelain plate, and check the shape of the ginseng strips or ginseng segments. There should be no broken pieces or fragments, and no other substances. 6.2.3 Smell Take 3 bags of ginseng strips, ginseng fruit tea, multi-dimensional ginseng fruit tea, and red strip ginseng tea, add 100ml of hot water respectively, stir to make them gradually dissolve, and taste them with your mouth. There should be no smell different from the original product. Take a few slices of ginseng and taste them at the same time. There should be no smell different from the original product or not authentic. The most human only wine calls its spine, tastes its ball with mouth, and shall not have the smell different from this product. 6.2.4 Box solution
3 bags of human dream benzene, human fruit tea, multi-dimensional personality fruit, red jing adult tea, respectively, add 80C hot water 100ml., stir for 5min, and the meat is completely decomposed and clarified. There shall be no confusion. There shall be no condensation or other foreign matter: 6.3 Physical and chemical inspection
6.3.1 Poor quality
Collect human tea, ginseng fruit tea, multi-continent human fruit tea, red jing adult tea, human medicine egg tablets 5 bags, monthly sensitivity 3.01 body size is about to be used, must meet the standard requirements, human wine is engraved with 1ml. The weight is not lower than the standard. 6-3-2 Water secretion is
According to the determination method of GH/T5005.3 specification. 6.3.3 Determination of quinoline content
The determination method is shown in Appendix A. 6.3.5 Determination of ginseng total components
The determination method is shown in Appendix II. 6.3.6 Ginseng tablets should contain quinoline. G/TSUU. Determination is carried out as follows:
6.3.7 Determination of hemoglobin content
6 3.7.1 Apparatus
The test tube should be exposed to light.
6.3.7.2 Reagents
NY3181997
Hongyuan Tianyou for oral administration: provided by China Food and Drug Administration. 6.3.7.4 Preparation of selected products
Accurately weigh 3 samples of the selected products and dissolve them in water. Add the samples into a funnel and degrease them by vibration for 3 times with acetaldehyde. The amounts of acetaldehyde used are 40.30 and 2ml respectively. Discard acetaldehyde: remove 4.3.21ml of acetaldehyde by water and n-butanol for 3 times. Combine the acetaldehyde and dilute to 1( ml, hold and prepare 6.3.7.5 Colorimetric test sample. Prepare 20 ml of the sample and 10 quantification reagents respectively. The well is empty. Use ultraviolet visible spectrometer to measure the relative humidity at a wavelength of 2 μm. According to the formula: Where: A - weigh the entire volume of the reference substance, H - sample absorbance: C - red absorbance of the reference substance: 6.3.82 Determination of the content of fluorine and pentafluorocarbon residues 100
Take about 56 of the product: Determine by weighing according to the method specified in GBF5:M9.19. 6.3.9 Determination of the average value
Take about 2 of the product, and adjust the amount according to the method specified in G/T5009.11. 6.3.10 Determination of lead
Long product about 8+ precision weighing, GB/T5009.12 Heat determination method 6.3.11 Determination
Take about 2 of the product, and adjust the amount according to the method specified in (15/[5099. 14 regulations, treatment and parallel determination 6.3-17 (confirm 4 element 33 determination
take about 2 islands of sample, help secrete the amount, and then measure according to G1/=2330. 6.3.13 Determination of riboflavin
item 2%, weigh the instrument and measure according to G/12391. 6.3.14 Determination of total ascorbic acid
collect about 28 of the sample, accurately weigh the range, calibrate the method specified in G/712392, select and specify, 1
6.3.15 human quality acid test method
r318-1997
refer to GB/T10345.1 and GB/T10346 for inspection 5-3-16 reduction of the product ||tt| |Take 20 μg of this product and weigh it. Determine it according to the method specified in GB/12396. 6.4 Microbiological test
6.4.1 Determination of total bacteria
Determine it according to the method specified in GB479-3-2. 6.4.2 Determination of coliform group
Determine it according to the method specified in GB479-3-3.
6.4.3 Determination of free bacteria
Determine it according to the method specified in GB479.15
7 Inspection rules
7.1 Factory inspection
7.1.1 Before the first batch of products leave the factory, the quality inspection department of the production factory shall conduct inspection according to the physical and chemical indicators in 4.2 of this standard. Only qualified products can leave the factory. 7.1.2 During the inspection: the water content, total ginseng content and microbial indicators of the physical and chemical index are mandatory items. Other items can be inspected irregularly.
7.2 Type inspection
When one of the following conditions occurs, a type inspection shall be generally carried out: a) The product must be tested for production; b) After the production is completed, if the materials and production process have changed significantly, which may affect the product quality; c) During the production, the product shall be inspected periodically or after a certain amount of production (each batch is more than 1CC box); e) When the product is stopped for a long time, the factory shall conduct a type inspection; e) When the inspection result is significantly different from the previous type inspection; F) When the national quality supervision agency proposes a requirement for type inspection. 7.3 Special prohibitions
For the mandatory items specified in the regulations, except for moisture, before selling, the pesticide residues must be delivered in the form of method, basic quantity, lead, bell and biological sound mark. If any one of them exceeds the standard, the product will be judged as unqualified: then double sampling will be taken from the product for re-inspection. If the company finds that the product is qualified, the company can judge the product as qualified. If there is one non-conformity, the batch of products will be judged as unqualified. If pathogenic bacteria are detected, re-calibration will not be promoted, and the batch production capacity will be waived or the new packaging will be changed. Under the supervision of the quality supervision department, the products will be sold on the spot. 8. Marking, packaging, transportation and storage
8.1 Marking
8.1.1 Product marking
The product marking must include: product name, trademark, specification, name, factory address, postal code, telephone number, method of use, production period, shelf life, production registration number, batch number, product conditions, etc. The labeling and marking must comply with the provisions of GB9. The labeling and marking must comply with the provisions of GB34.
81.2 Packaging
The packaging label must include the product name, registered trademark, quantity, factory address, postal code, telephone number, production date, shelf life, batch number, and symbols such as "handle with care", "moisture-proof", "anti-smoke", etc.: it must comply with the provisions of GB1. 8.2 Packaging
Ginseng tea should be packed with moisture-proof paper or sealed with steel-plastic composite film. The product logo should be printed on the outside of the packaging box. Each box must have: product certificate, product manual, packing list, etc.
8.3 Transportation
NY 3181997
During transportation, please pay attention to the precautions, protection from sunlight and handle with care. No harmful, abrasive or abrasive objects should be mixed with the goods for transportation.
8.4 Storage
Store in a dark, ventilated and dry warehouse. It can be stored at any time, but it should not be placed together with toxic, harmful or microbial items. The transportation period should not exceed the shelf life.
8.5 Shelf life
People's tea, ginseng fruit tea, multi-vitamin ginseng fruit tea, red urine ginseng strips and honey tablets, ginseng shelf life 4 halls, A1 principle
NY318-1997
Appendix A
(Appendix to the standard)
Determination method of total sugar in ginseng products
3.5-A whistle of water and reducing sugar are heated to produce brown-red amino compounds. Within a certain range, the amount of aptasensor and the color intensity of the reaction solution are proportional. The amount of aptasensor in the sample can be determined by colorimetry. A2 Only for spectrophotometer
A3 Reagents
Phenol, potassium iodide, 3-nitro salicylate, hydrochloric acid, sodium oxide, potassium iodide, phenol, ethanol, glacial acetic acid, sodium bisulfite, analytical grade.
A3.1 Acid solution: distill 10% ethanol to collect the acid solution. Take 10% sodium oxide solution and add water to dilute to 1L. A3.3 Chemical reagent: Take 10% ethanol and dilute to 1L. A3.4 Acid indicator: add 0.18% ethanol to dilute to 100L. A3.5Rz solution: Take 100mL of 95% 7 and add 13.3L of water to obtain A3.6C. Weigh 1% glucose in the standard melt [dry to constant weight at 105°C, dissolve with a small amount of distilled water and weigh to make up to 10mL, keep in the tank for later use. A3.3.5-acetaldehyde reagent (DNS): A: Take 6.6g of the product in 15.2mL of 10% sodium hydroxide solution, dilute to 53mL, add 6.9g of sulfuric acid to the solution, and obtain B: Add 58% sodium hydroxide to 21% of sodium hydroxide to dissolve, then add 88% sodium dihydrate solution and mix.
Mix Liquid A and Liquid B to obtain a yellow reagent, put it in a colorimetric test bottle, and place it in a room temperature for 7-10 days before use: A4 Analysis Steps
A4.1 Preparation of glucose and standard reagent
Take 9 ml of glucose and add various reagents in the order of Table A1, Table A1
Additional order of various reagents
Total amount g
Total amount of liquid glucose, ml. ... 1 (Finish)
Immediately pour running cold water over the above tubes and perform colorimetric determination at a wavelength of 52μ1 by visible light spectrophotometry with the accompanying reagent as blank, glucose as yellow coordinate, light intensity as ordinate, and age standard A4.2 Correction and total determination of sample system
A4.2.1 Extraction of reduced sulfur from selected samples
Pipette 2 ml of sample into a 0.5 ml cup, add two drops of solution in order, mix, keep in constant temperature water for 30min, filter, add the residue twice with ethanol, wash together, evaporate the ethanol, dissolve in a small amount of distilled water and make up to 100 ml for use.
A4.2-2 Total hydrolysis extraction of samples
Accurately weigh 1 ml of sample and place it in a large test tube. Add 20 ml of acid and 1 ml of natural water and mix. Heat in a water bath for 3 minutes. Then use potassium iodide solution to check the degree of hydrolysis. If the hydrolysis is complete, no blue color (pink) will appear. After cooling, add acid indicator. 0% sodium oxide is added to the reagent until it turns slightly red and the concentration is adjusted to 10cm. Use 100mM water to calibrate the concentration. A4.2.3 Take a sample with a certain content of reducing sugar and add reagents according to Table A. A2.2. Sample: 1 ml. Add water. uL. Add glucose to the solution in flowing water. Then measure the absorbance of each tube according to the preparation method of the standard curve. Find the corresponding reducing sugar content on the standard curve. Calculate the total amount of reducing sugar in the sample by connecting formula (A1) and formula (A). Reduction (
reduction grinding? product dilution times
product quality
total description %) = hydrolysis reduction secretion point total sample cell number × 100 product appropriateness
.....(Al)
1 Principle
B
(Standard Appendix)
Content determination method of ginseng total saponin
The distribution coefficient of ginseng in n-butanol is larger than that in water. After desorption, the saponin is purified by n-butanol extraction. Ginseng saponin reacts with pre-acid-scented aldehyde to develop color, with a slight peak at 141 nm, which is then concentrated under a constant concentration of -100 nm. B2 Instrument
B2.1 Visible spectrophotometer.
H2.2 Extractor 5,
B2-3 super-production.
3.1 Aldehyde, alcohol. Normal alcohol sulfur (specific gravity 1.84~1.86, anhydrous alcohol, aromatic aldehyde are all externally purified, B3.2 Signature Re for products: H medium chaos drug biological product granules determined by Hong, 33 children take 0 water alcohol to 1m can be used for preparation)
.4 General acid solution: take the fluctuating acid grade condensate into appropriate water. Cool to room temperature, add water to dilute to W, shake for use.
B35 for the product to the appropriate plate in the refined product 20g10 through the plate bottle and dissolve to the scale, shake and hook each use.
B4 Determination method
B4.1 Preparation of sample solution
B4-1-1 Tea
Take 2 ml of the sample and weigh it. Put it in a 1ml cup. Dissolve it in 40:1 steamed water. Transfer it to a 250ml filter and add 40:1 steam to increase the moisture content. Then add 30% acetaldehyde to the distilled water bucket and shake it once. Then add 250ml of water and shake it once. Drain with lysate for 1 time, discard the water layer after separation. Take the blood from the positive layer, dissolve it in water and then dry it. After the residual alcohol is dissolved with a clean towel, transfer it to a container. Use the image to mark the scale. Prepare for use: B4.1.2. Collect the product in a 5-meter box, bake at 70°C for 10 minutes, weigh it accurately after grinding, wrap it with a dropper, put it in a Soxhlet collector, add 1 ml of the flow-through, remove the ethyl acetate, separate the ethyl acetate, and add a triangle. After mixing with 1m, add 1/2 of the amount of n-butanol saturated with water and extract 3rrin, centrifuge and absorb the upper digestion. Combine the n-butanol and add 2 times the amount of distilled water: place in a bucket, evenly divide into layers, remove the water layer, take the upper alcohol layer, dissolve it in methanol, transfer it to 1m, dilute it with 1/2 alcohol to the desired concentration, shake and set aside. B4.1-3 More wines
Extract the original product 1 Place in evaporator III, distill water for ten times. Distill the remaining pieces The tumor water was divided into 6 parts and the liquid was taken out in batches. The blood was evaporated twice with 20 ml of ethanol and then adjusted. 3.31 and 20 ml of aldehyde were taken out three times. The large amount of aldehyde was discarded. Then 30 and 2520 ml of water and n-butanol were extracted once. The n-butanol was separated and the water layer was discarded. The n-butanol layer was taken out and the liquid was evaporated. The residual sound was removed and the solution was transferred to the 10-ml water container.Compounds, within a certain range, the amount of appropriate essence and the color intensity of the reaction solution are proportional, and the amount of shrinkage in the sample can be determined by colorimetry. A2 only
spectrophotometer
A3 reagents
phenol, potassium tack, 3,-nitrosalicylic acid, hydrochloric acid, sodium hydroxide, potassium iodide, flagellin, phenol, ethanol, glacial acetic acid, sodium bisulfite, analytical grade.
A3.1 Acid solution: Collect 1 mL of 5℃ distilled water. 3.210 Sodium oxide solution Take 10 mL of oxidizing agent and add water to 1 mL of hot water. A3.3 Chemical evaluation solution: Take 1 mL of 1 mL of dry water, add ice gel 2 mL and dilute with distilled water to 100 mL. A3.4 Acid indicator: Weigh 0.18% ethanol solution and dilute to 100 mL. A3.5 Rz solution: Take 7100 mL of 95% ethanol and add 13.3 L of water to obtain A3.6C.% standard melt Weigh 1 mL of glucose [2 mL] and dry to constant weight at 105 °C. Dissolve with a small amount of distilled water and weigh to a fixed volume of ) mL. Keep in the tank for later use. A3.5-acetic acid reagent (DNS design): A: Take 6.6 g of the product in 15.2 mL of 10% sodium hydroxide solution, dilute to 53 mL, add 6.9 g of sulfuric acid, and the mixture is ready. B: Add 58 g of sodium dextrose into 21% 10% sodium hydroxide solution to dissolve it, then add 8.81% sodium dihydrate solution and mix.
Mix Liquid A and Liquid B to obtain a yellow reagent, put it in a colorimetric test bottle, and place it in a room temperature for 7-10 days before use: A4 Analysis Steps
A4.1 Preparation of glucose and standard reagent
Take 9 ml of glucose and add various reagents in the order of Table A1, Table A1
Additional order of various reagents
Total amount g
Total amount of liquid glucose, ml. ... 1 (Finish)
Immediately pour running cold water over the above tubes and perform colorimetric determination at a wavelength of 52μ1 by visible light spectrophotometry with the accompanying reagent as blank, glucose as yellow coordinate, light intensity as ordinate, and age standard A4.2 Correction and total determination of sample system
A4.2.1 Extraction of reduced sulfur from selected samples
Pipette 2 ml of sample into a 0.5 ml cup, add two drops of solution in order, mix, keep in constant temperature water for 30min, filter, add the residue twice with ethanol, wash together, evaporate the ethanol, dissolve in a small amount of distilled water and make up to 100 ml for use.
A4.2-2 Total hydrolysis extraction of samples
Accurately weigh 1 ml of sample and place it in a large test tube. Add 20 ml of acid and 1 ml of natural water and mix. Heat in a water bath for 3 minutes. Then use potassium iodide solution to check the degree of hydrolysis. If the hydrolysis is complete, no blue color (pink) will appear. After cooling, add acid indicator. 0% sodium oxide is added to the reagent until it turns slightly red and the concentration is adjusted to 10cm. Use 100mM water to calibrate the concentration. A4.2.3 Take a sample with a certain content of reducing sugar and add reagents according to Table A. A2.2. Sample: 1 ml. Add water. uL. Add glucose to the solution in flowing water. Then measure the absorbance of each tube according to the preparation method of the standard curve. Find the corresponding reducing sugar content on the standard curve. Calculate the total amount of reducing sugar in the sample by connecting formula (A1) and formula (A). Reduction (
reduction grinding? product dilution times
product quality
total description %) = hydrolysis reduction secretion point total sample cell number × 100 product appropriateness
.....(Al)
1 Principle
B
(Standard Appendix)
Content determination method of ginseng total saponin
The distribution coefficient of ginseng in n-butanol is larger than that in water. After desorption, the saponin is purified by n-butanol extraction. Ginseng saponin reacts with pre-acid-scented aldehyde to develop color, with a slight peak at 141 nm, which is then concentrated under a constant concentration of -100 nm. B2 Instrument
B2.1 Visible spectrophotometer.
H2.2 Extractor 5,
B2-3 super-production.
3.1 Aldehyde, alcohol. Normal alcohol sulfur (specific gravity 1.84~1.86, anhydrous alcohol, aromatic aldehyde are all externally purified, B3.2 Signature Re for products: H medium chaos drug biological product granules determined by Hong, 33 children take 0 water alcohol to 1m can be used for preparation)
.4 General acid solution: take the fluctuating acid grade condensate into appropriate water. Cool to room temperature, add water to dilute to W, shake for use.
B35 for the product to the appropriate plate in the refined product 20g10 through the plate bottle and dissolve to the scale, shake and hook each use.
B4 Determination method
B4.1 Preparation of sample solution
B4-1-1 Tea
Take 2 ml of the sample and weigh it. Put it in a 1ml cup. Dissolve it in 40:1 steamed water. Transfer it to a 250ml filter and add 40:1 steam to increase the moisture content. Then add 30% acetaldehyde to the distilled water bucket and shake it once. Then add 250ml of water and shake it once. Drain with lysate for 1 time, discard the water layer after separation. Take the blood from the positive layer, dissolve it in water and then dry it. After the residual alcohol is dissolved with a clean towel, transfer it to a container. Use the image to mark the scale. Prepare for use: B4.1.2. Collect the product in a 5-meter box, bake at 70°C for 10 minutes, weigh it accurately after grinding, wrap it with a dropper, put it in a Soxhlet collector, add 1 ml of the flow-through, remove the ethyl acetate, separate the ethyl acetate, and add a triangle. After mixing with 1m, add 1/2 of the amount of n-butanol saturated with water and extract 3rrin, centrifuge and absorb the upper digestion. Combine the n-butanol and add 2 times the amount of distilled water: place in a bucket, evenly divide into layers, remove the water layer, take the upper alcohol layer, dissolve it in methanol, transfer it to 1m, dilute it with 1/2 alcohol to the desired concentration, shake and set aside. B4.1-3 More wines
Extract the original product 1 Place in evaporator III, distill water for ten times. Distill the remaining pieces The tumor water was divided into 6 parts and the liquid was taken out in batches. The blood was evaporated twice with 20 ml of ethanol and then adjusted. 3.31 and 20 ml of aldehyde were taken out three times. The large amount of aldehyde was discarded. Then 30 and 2520 ml of water and n-butanol were extracted once. The n-butanol was separated and the water layer was discarded. The n-butanol layer was taken out and the liquid was evaporated. The residual sound was removed and the solution was transferred to the 10-ml water container.Compounds, within a certain range, the amount of appropriate essence and the color intensity of the reaction solution are proportional, and the amount of shrinkage in the sample can be determined by colorimetry. A2 only
spectrophotometer
A3 reagents
phenol, potassium tack, 3,-nitrosalicylic acid, hydrochloric acid, sodium hydroxide, potassium iodide, flagellin, phenol, ethanol, glacial acetic acid, sodium bisulfite, analytical grade.
A3.1 Acid solution: Collect 1 mL of 5℃ distilled water. 3.210 Sodium oxide solution Take 10 mL of oxidizing agent and add water to 1 mL of hot water. A3.3 Chemical evaluation solution: Take 1 mL of 1 mL of dry water, add ice gel 2 mL and dilute with distilled water to 100 mL. A3.4 Acid indicator: Weigh 0.18% ethanol solution and dilute to 100 mL. A3.5 Rz solution: Take 7100 mL of 95% ethanol and add 13.3 L of water to obtain A3.6C.% standard melt Weigh 1 mL of glucose [2 mL] and dry to constant weight at 105 °C. Dissolve with a small amount of distilled water and weigh to a fixed volume of ) mL. Keep in the tank for later use. A3.5-acetic acid reagent (DNS reagent): A: Take 6.6 g of the product in 15.2 mL of 10% sodium hydroxide solution, dilute to 53 mL, add 6.9 g of sulfuric acid, and the mixture is ready. B: Add 58 g of sodium dextrose into 10% sodium hydroxide solution to dissolve it, then add 8.81% sodium dihydrate solution and mix.
Mix Liquid A and Liquid B to obtain a yellow reagent, put it in a colorimetric test bottle, and place it in a room temperature for 7-10 days before use: A4 Analysis Steps
A4.1 Preparation of glucose and standard reagent
Take 9 ml of glucose and add various reagents in the order of Table A1, Table A1
Additional order of various reagents
Total amount g
Total amount of liquid glucose, ml. ... 1 (Finish)
Immediately pour running cold water over the above tubes and perform colorimetric determination at a wavelength of 52μ1 by visible light spectrophotometry with the accompanying reagent as blank, glucose as yellow coordinate, light intensity as ordinate, and age standard A4.2 Correction and total determination of sample system
A4.2.1 Extraction of reduced sulfur from selected samplesWww.bzxZ.net
Pipette 2 ml of sample into a 0.5 ml cup, add two drops of solution in order, mix, keep in constant temperature water for 30min, filter, add the residue twice with ethanol, wash together, evaporate the ethanol, dissolve in a small amount of distilled water and make up to 100 ml for use.
A4.2-2 Total hydrolysis extraction of samples
Accurately weigh 1 ml of sample and place it in a large test tube. Add 20 ml of acid and 1 ml of natural water and mix. Heat in a water bath for 3 minutes. Then use potassium iodide solution to check the degree of hydrolysis. If the hydrolysis is complete, no blue color (pink) will appear. After cooling, add acid indicator. 0% sodium oxide is added to the reagent until it turns slightly red and the concentration is adjusted to 10cm. Use 100mM water to calibrate the concentration. A4.2.3 Take a sample with a certain content of reducing sugar and add reagents according to Table A. A2.2. Sample: 1 ml. Add water. uL. Add glucose to the solution in flowing water. Then measure the absorbance of each tube according to the preparation method of the standard curve. Find the corresponding reducing sugar content on the standard curve. Calculate the total amount of reducing sugar in the sample by connecting formula (A1) and formula (A). Reduction (
reduction grinding? product dilution times
product quality
total description %) = hydrolysis reduction secretion point total sample cell number × 100 product appropriateness
.....(Al)
1 Principle
B
(Standard Appendix)
Content determination method of ginseng total saponin
The distribution coefficient of ginseng in n-butanol is larger than that in water. After desorption, the saponin is purified by n-butanol extraction. Ginseng saponin reacts with pre-acid-scented aldehyde to develop color, with a slight peak at 141 nm, which is then concentrated under a constant concentration of -100 nm. B2 Instrument
B2.1 Visible spectrophotometer.
H2.2 Extractor 5,
B2-3 super-production.
3.1 Aldehyde, alcohol. Normal alcohol sulfur (specific gravity 1.84~1.86, anhydrous alcohol, aromatic aldehyde are all externally purified, B3.2 Signature Re for products: H medium chaos drug biological product granules determined by Hong, 33 children take 0 water alcohol to 1m can be used for preparation)
.4 General acid solution: take the fluctuating acid grade condensate into appropriate water. Cool to room temperature, add water to dilute to W, shake for use.
B35 for the product to the appropriate plate in the refined product 20g10 through the plate bottle and dissolve to the scale, shake and hook each use.
B4 Determination method
B4.1 Preparation of sample solution
B4-1-1 Tea
Take 2 ml of the sample and weigh it. Put it in a 1ml cup. Dissolve it in 40:1 steamed water. Transfer it to a 250ml filter and add 40:1 steam to increase the moisture content. Then add 30% acetaldehyde to the distilled water bucket and shake it once. Then add 250ml of water and shake it once. Drain with lysate for 1 time, discard the water layer after separation. Take the blood from the positive layer, dissolve it in water and then dry it. After the residual alcohol is dissolved with a clean towel, transfer it to a container. Use the image to mark the scale. Prepare for use: B4.1.2. Collect the product in a 5-meter box, bake at 70°C for 10 minutes, weigh it accurately after grinding, wrap it with a dropper, put it in a Soxhlet collector, add 1 ml of the flow-through, remove the ethyl acetate, separate the ethyl acetate, and add a triangle. After mixing with 1m, add 1/2 of the amount of n-butanol saturated with water and extract 3rrin, centrifuge and absorb the upper digestion. Combine the n-butanol and add 2 times the amount of distilled water: place in a bucket, evenly divide into layers, remove the water layer, take the upper alcohol layer, dissolve it in methanol, transfer it to 1m, dilute it with 1/2 alcohol to the desired concentration, shake and set aside. B4.1-3 More wines
Extract the original product 1 Place in evaporator III, distill water for ten times. Distill the remaining pieces The tumor water was divided into 6 parts and the liquid was taken out in batches. The blood was evaporated twice with 20 ml of ethanol and then adjusted. 3.31 and 20 ml of aldehyde were taken out three times. The large amount of aldehyde was discarded. Then 30 and 2520 ml of water and n-butanol were extracted once. The n-butanol was separated and the water layer was discarded. The n-butanol layer was taken out and the liquid was evaporated. The residual sound was removed and the solution was transferred to the 10-ml water container.IT
Pass water, 0
Absorbance
NY318—1997
Table A1 (end)
Immediately flow cold water except
Use visible light spectrophotometry to colorimetrically determine the absorbance of the above tubes at a wavelength of 52μ1. The accompanying reagent is used as the blank, glucose as the yellow coordinate, and the vertical coordinate is the ordinate. The standard is A4.2 Sample system correction and total determination
A4.2.1 Extraction of reduced sulfur in selected samples
Pipette 2 ml of sample into a 0.5 ml cup, add two drops of solution in sequence, mix, and keep in constant temperature water for 30min, filter, add the residue to the top of the cup twice with ethanol, wash and combine, evaporate the ethanol, dissolve in a small amount of distilled water and make up to 100ml for use.
A4.2-2 Total hydrolysis extraction of samples
Accurately weigh 1 ml of sample and place it in a large test tube. Add 20 ml of acid and 1 ml of natural water and mix. Heat in a water bath for 3 minutes. Then use potassium iodide solution to check the degree of hydrolysis. If the hydrolysis is complete, no blue color (pink) will appear. After cooling, add acid indicator. 0% sodium oxide is added to the reagent until it turns slightly red and the concentration is adjusted to 10cm. Use 100mM water to calibrate the concentration. A4.2.3 Take a sample with a certain content of reducing sugar and add reagents according to Table A. A2.2. Sample: 1 ml. Add water. uL. Add glucose to the solution in flowing water. Then measure the absorbance of each tube according to the preparation method of the standard curve. Find the corresponding reducing sugar content on the standard curve. Calculate the total amount of reducing sugar in the sample by connecting formula (A1) and formula (A). Reduction (
reduction grinding? product dilution times
product quality
total description %) = hydrolysis reduction secretion point total sample cell number × 100 product appropriateness
.....(Al)
1 Principle
B
(Standard Appendix)
Content determination method of ginseng total saponin
The distribution coefficient of ginseng in n-butanol is larger than that in water. After desorption, the saponin is purified by n-butanol extraction. Ginseng saponin reacts with pre-acid-scented aldehyde to develop color, with a slight peak at 141 nm, which is then concentrated under a constant concentration of -100 nm. B2 Instrument
B2.1 Visible spectrophotometer.
H2.2 Extractor 5,
B2-3 super-production.
3.1 Aldehyde, alcohol. Normal alcohol sulfur (specific gravity 1.84~1.86, anhydrous alcohol, aromatic aldehyde are all externally purified, B3.2 Signature Re for products: H medium chaos drug biological product granules determined by Hong, 33 children take 0 water alcohol to 1m can be used for preparation)
.4 General acid solution: take the fluctuating acid grade condensate into appropriate water. Cool to room temperature, add water to dilute to W, shake for use.
B35 for the product to the appropriate plate in the refined product 20g10 through the plate bottle and dissolve to the scale, shake and hook each use.
B4 Determination method
B4.1 Preparation of sample solution
B4-1-1 Tea
Take 2 ml of the sample and weigh it. Put it in a 1ml cup. Dissolve it in 40:1 steamed water. Transfer it to a 250ml filter and add 40:1 steam to increase the moisture content. Then add 30% acetaldehyde to the distilled water bucket and shake it once. Then add 250ml of water and shake it once. Drain with lysate for 1 time, discard the water layer after separation. Take the blood from the positive layer, dissolve it in water and then dry it. After the residual alcohol is dissolved with a clean towel, transfer it to a container. Use the image to mark the scale. Prepare for use: B4.1.2. Collect the product in a 5-meter box, bake at 70°C for 10 minutes, weigh it accurately after grinding, wrap it with a dropper, put it in a Soxhlet collector, add 1 ml of the flow-through, remove the ethyl acetate, separate the ethyl acetate, and add a triangle. After mixing with 1m, add 1/2 of the amount of n-butanol saturated with water and extract 3rrin, centrifuge and absorb the upper digestion. Combine the n-butanol and add 2 times the amount of distilled water: place in a bucket, evenly divide into layers, remove the water layer, take the upper alcohol layer, dissolve it in methanol, transfer it to 1m, dilute it with 1/2 alcohol to the desired concentration, shake and set aside. B4.1-3 More wines
Extract the original product 1 Place in evaporator III, distill water for ten times. Distill the remaining pieces The tumor water was divided into 6 parts and the liquid was taken out in batches. The blood was evaporated twice with 20 ml of ethanol and then adjusted. 3.31 and 20 ml of aldehyde were taken out three times. The large amount of aldehyde was discarded. Then 30 and 2520 ml of water and n-butanol were extracted once. The n-butanol was separated and the water layer was discarded. The n-butanol layer was taken out and the liquid was evaporated. The residual sound was removed and the solution was transferred to the 10-ml water container.IT
Pass water, 0
Absorbance
NY318—1997
Table A1 (end)
Immediately flow cold water except
Use visible light spectrophotometry to colorimetrically determine the absorbance of the above tubes at a wavelength of 52μ1. The accompanying reagent is used as the blank, glucose as the yellow coordinate, and the vertical coordinate is the ordinate. The standard is A4.2 Sample system correction and total determination
A4.2.1 Extraction of reduced sulfur in selected samples
Pipette 2 ml of sample into a 0.5 ml cup, add two drops of solution in sequence, mix, and keep in constant temperature water for 30min, filter, add the residue to the top of the cup twice with ethanol, wash and combine, evaporate the ethanol, dissolve in a small amount of distilled water and make up to 100ml for use.
A4.2-2 Total hydrolysis extraction of samples
Accurately weigh 1 ml of sample and place it in a large test tube. Add 20 ml of acid and 1 ml of natural water and mix. Heat in a water bath for 3 minutes. Then use potassium iodide solution to check the degree of hydrolysis. If the hydrolysis is complete, no blue color (pink) will appear. After cooling, add acid indicator. 0% sodium oxide is added to the reagent until it turns slightly red and the concentration is adjusted to 10cm. Use 100mM water to calibrate the concentration. A4.2.3 Take a sample with a certain content of reducing sugar and add reagents according to Table A. A2.2. Sample: 1 ml. Add water. uL. Add glucose to the solution in flowing water. Then measure the absorbance of each tube according to the preparation method of the standard curve. Find the corresponding reducing sugar content on the standard curve. Calculate the total amount of reducing sugar in the sample by connecting formula (A1) and formula (A). Reduction (
reduction grinding? product dilution times
product quality
total description %) = hydrolysis reduction secretion point total sample cell number × 100 product appropriateness
.....(Al)
1 Principle
B
(Standard Appendix)
Content determination method of ginseng total saponin
The distribution coefficient of ginseng in n-butanol is larger than that in water. After desorption, the saponin is purified by n-butanol extraction. Ginseng saponin reacts with pre-acid-scented aldehyde to develop color, with a slight peak at 141 nm, which is then concentrated under a constant concentration of -100 nm. B2 Instrument
B2.1 Visible spectrophotometer.
H2.2 Extractor 5,
B2-3 super-production.
3.1 Aldehyde, alcohol. Normal alcohol sulfur (specific gravity 1.84~1.86, anhydrous alcohol, aromatic aldehyde are all externally purified, B3.2 Signature Re for products: H medium chaos drug biological product granules determined by Hong, 33 children take 0 water alcohol to 1m can be used for preparation)
.4 General acid solution: take the fluctuating acid grade condensate into appropriate water. Cool to room temperature, add water to dilute to W, shake for use.
B35 for the product to the appropriate plate in the refined product 20g10 through the plate bottle and dissolve to the scale, shake and hook each use.
B4 Determination method
B4.1 Preparation of sample solution
B4-1-1 Tea
Take 2 ml of the sample and weigh it. Put it in a 1ml cup. Dissolve it in 40:1 steamed water. Transfer it to a 250ml filter and add 40:1 steam to increase the moisture content. Then add 30% acetaldehyde to the distilled water bucket and shake it once. Then add 250ml of water and shake it once. Drain with lysate for 1 time, discard the water layer after separation. Take the blood from the positive layer, dissolve it in water and then dry it. After the residual alcohol is dissolved with a clean towel, transfer it to a container. Use the image to mark the scale. Prepare for use: B4.1.2. Collect the product in a 5-meter box, bake at 70°C for 10 minutes, weigh it accurately after grinding, wrap it with a dropper, put it in a Soxhlet collector, add 1 ml of the flow-through, remove the ethyl acetate, separate the ethyl acetate, and add a triangle. After mixing with 1m, add 1/2 of the amount of n-butanol saturated with water and extract 3rrin, centrifuge and absorb the upper digestion. Combine the n-butanol and add 2 times the amount of distilled water: place in a bucket, evenly divide into layers, remove the water layer, take the upper alcohol layer, dissolve it in methanol, transfer it to 1m, dilute it with 1/2 alcohol to the desired concentration, shake and set aside. B4.1-3 More wines
Extract the original product 1 Place in evaporator III, distill water for ten times. Distill the remaining pieces The tumor water was divided into 6 parts and the liquid was taken out in batches. The blood was evaporated twice with 20 ml of ethanol and then adjusted. 3.31 and 20 ml of aldehyde were taken out three times. The large amount of aldehyde was discarded. Then 30 and 2520 ml of water and n-butanol were extracted once. The n-butanol was separated and the water layer was discarded. The n-butanol layer was taken out and the liquid was evaporated. The residual sound was removed and the solution was transferred to the 10-ml water container.1.2 The emergency tablets were collected in a 5-well box and dried at 70 °C. After grinding, they were accurately weighed and wrapped in a grade. They were placed in a Soxhlet collector. A flow cytometer was added to remove all ethyl acetate. The ethyl acetate was removed and the mixture was dried. After that, a 1 ml stopper was added to the triangular tube. After mixing with 1 ml of water, 1 ml of n-butanol was saturated with water and the mixture was extracted for 3 min. The upper digestate was absorbed by centrifugation. A total of 400 ml of n-butanol was added and 2 times the amount of distilled water was added. The mixture was placed in a hopper and evenly separated into layers. The water layer was removed. The upper alcohol layer was taken and dissolved in methanol on a 4% sodium hydroxide solution. The mixture was transferred to a 1 ml solution and diluted to standard concentration with 10% ethanol. Shake the mixture. , set aside. B4.1-3 alcohol
The original product is placed in evaporator III, and water is evaporated for ten minutes. The remaining pieces are evaporated for 6 hours! The solution is taken out in batches, and the positive liquid is transferred to the separator. The positive liquid is washed with 20m. The evaporation blood is rinsed twice, and the liquid is adjusted. Add 3.31, 20r1. Take out three times at a rate of 1, discard the large 7 secret, and then use water and n-butanol 30, 2520m[. Collect once, separate into - butanol, and evaporate 1 volume of water. Keep stratification, discard the water layer, take the positive layer 1, and then perform the technique. The residual sound is transferred to the store for 10m.1.2 The emergency tablets were collected in a 5-well box and dried at 70 °C. After grinding, they were accurately weighed and wrapped in a grade. They were placed in a Soxhlet collector. A flow cytometer was added to remove all ethyl acetate. The ethyl acetate was removed and the mixture was dried. After that, a 1 ml stopper was added to the triangular tube. After mixing with 1 ml of water, 1 ml of n-butanol was saturated with water and the mixture was extracted for 3 min. The upper digestate was absorbed by centrifugation. A total of 400 ml of n-butanol was added and 2 times the amount of distilled water was added. The mixture was placed in a hopper and evenly separated into layers. The water layer was removed. The upper alcohol layer was taken and dissolved in methanol on a 4% sodium hydroxide solution. The mixture was transferred to a 1 ml solution and diluted to standard concentration with 10% ethanol. Shake the mixture. , set aside. B4.1-3 alcohol
The original product is placed in evaporator III, and water is evaporated for ten minutes. The remaining pieces are evaporated for 6 hours! The solution is taken out in batches, and the positive liquid is transferred to the separator. The positive liquid is washed with 20m. The evaporation blood is rinsed twice, and the liquid is adjusted. Add 3.31, 20r1. Take out three times at a rate of 1, discard the large 7 secret, and then use water and n-butanol 30, 2520m[. Collect once, separate into - butanol, and evaporate 1 volume of water. Keep stratification, discard the water layer, take the positive layer 1, and then perform the technique. The residual sound is transferred to the store for 10m.
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