Some standard content:
National Metrology Verification Regulation of the People's Republic of China JJG375-1996
Ullrarvioletvisiblerange
SpectrophotometerinSingleBeam1996-12-31Promulgated
Implemented onJune1997-06-01
State Bureau of Technical Supervision Issued
J.IG 375-1996
Verification Regulation
ofUltravioletVisibleRangeSpectrophotumelerinSingleBeam JJG375-1996
Replaces JJG375-1985
This calibration regulation was approved by the State Technical Supervision Bureau on December 31, 1996, and will be implemented on June 1, 1997
Responsible unit: Heilongjiang Provincial Technical Supervision Bureau Drafting unit: Heilongjiang Price and Measurement Inspection and Testing Institute The technical drafting unit of this regulation is responsible for the interpretation of this regulation. The main drafter of this regulation!
Ye Jun'an
Zhang Xiulan
Additional drafter:
Yang Najun
Ji Boliang
J.[G 375—1996
(Heilongjiang Province Metrology Verification and Testing Institute)
(Heilongjiang Province Metrology Verification and Testing Institute)
(Heilongjiang Province Technical Supervision and Inspection Institute) (National Standard Material Research Center)
(Shanghai Metrology and Testing Technology Institute) III
Technical requirements
Verification items and verification methods
Verification result processing and verification cycle
Preparation of test solution
Appendix 2
Appendix 3
Test wavelength table for low quartz mercury lamp
JJG375—1996
Record format of transmittance ratio of potassium dichromate standard solution at different temperatures at corresponding wavelength
Format of test result notice (back)
【5】
JJG 375-1996
Verification Procedure for Single-Beam UV-Vis Spectrophotometer This procedure is applicable to the verification of newly manufactured and used single-beam UV-Vis spectrophotometers with a wavelength range of 190-190 nm or with the above-mentioned region as the main spectrum. 1. Overview
Single-beam UV-Vis spectrophotometer [hereinafter referred to as the instrument] is an analytical calibrator that measures the properties and content of substances based on the absorption spectrum of the molecules of substances to the radiation (light) in the visible and ultraviolet regions and the L. Amberteer law. The mathematical expression of Lambert-Feier's method is: A=logtu/@)-logr=abt
In the formula, A is the absorbance of a substance:
is the monochromatic radiation intensity of the incident light;
is the monochromatic radiation intensity of the incident light;
is the transmittance of the substance;
is the absorption coefficient of the substance;
is the length of the optical path through the substance being measured;
is the concentration of the substance.
The instrument should consist of a light source system, a monochromator system, a sample chamber and a detection system. Technical requirements
1 The instrument should have the following signs: name, model, manufacturer, manufacturer's name, working power supply voltage and power
1.2 The instrument should be able to lie on the workbench stably, all fasteners should be well tightened, all adjustment knobs, buttons and switches should work properly, the connectors of the cable should be tightly matched, the instrument connection should be good, and the sample channel should be positioned properly.
1.3 The indicator scale is fine, uniform and clear, the digital display is complete, the operation part is stable, and there should be no jamming, jumping and display empty return.
1.4 The bottom of the sample chamber is well sealed and there is no light leakage: 1.5 When the instrument is in operation, the light source has no glare. The hydrogen lamp (hydrogen lamp) can be ignited normally, the laser of the instrument is placed at 581111, and the light of the stop band should be seen in the sample room. 2. Total stability
The stability of the instrument should meet the requirements of the table. Model
3. Wavelength accuracy and wavelength attenuation
JJ3751996
Fluid ( )
The instrument should meet the requirements of Table 2: Table 2
Wavelength.rm
200 --.3.50
350-500
700 --850
190-85
.? The constant value of the measured value should not exceed half of the absolute value of the measured value. 4 Sensitivity
The visible data of the slow-mirror type micro-device should be effective. Table 3
The total value/m
5 Minimum spectral width
Photoelectric type A, the minimum spectral width should not exceed the nominal value! .2 times. Then the transmission accuracy and the transmitted light weight
Photocurrent (3 mn)
Accuracy/m
Red-loaded phototube 0.06
ITG375—1996
6.1 Transmission accuracy of the slow-mirror type micro-device: the new one shall not exceed 15; the one in use and repair shall not exceed .7
6.? The grating type instrument transmittance accuracy should be in accordance with the requirements of Table 4
confidence level/(%)
6.3 The instrument transmittance repeatability should be accurate to the absolute value of the corresponding transmittance: For this purpose, the line levelness
and the line verticality of the instrument should meet the requirements of Table 5. Table 5
Test (automatic scanning)
Instrument noise will meet the requirements,
Type (automatic scanning)
Influence of power supply
Basic operating range (A)
The influence of power supply voltage (220+22)V on the instrument when the temperature is changed should meet the requirements, 3
10 Spurious emission rate
Change
1.1 Calibration type only Spurious emission rate: Newly manufactured instruments shall not exceed 0.6; Instruments in use and after repair Not more than (.8%.
1I.2 The stray radiation rate of the grating instrument shall meet the requirements of the clothing, Table 8
The matching of the absorption cell
The matching of the absorption cell shall meet the requirements of Table 9. Table 9
Absorption cell category
Insulation resistance
The insulation resistance of the instrument shall not be less than 5M
13 Verification environment conditions
Hunger length/nm
Verification conditions
Sensitive radiation rate/(%)
Matching error/()||t t||JJG375—1996
13.1 Temperature: 10～30℃ Relative humidity: not more than 85%, 13.2 Power supply: voltage (220±22)V: frequency (50±1)Hz13.3 The instrument should not be exposed to strong light, no strong magnetic field, electric field disturbance, no strong air flow, no gas modification: 14 Calibration equipment
14.t Voltage transformer: 500W; output 0--250V. 14.2 Frequency: 45-65Hz: accuracy ±0.5% AC voltage meter: 150--300V: accuracy 1.0. 14.3 Megaohmmeter, test voltage 50V: accuracy 1.0. Stopwatch: graduation value 0.18
15 Standard instruments and standard materials
15.1 Jasmine lamp.
15.2 Oxide glass filter, oxidation liquid, spectrum neodymium, zirconium glass filter. It is required to have wavelength standard values under 2nm and 5nm bandwidth,
15.3 0.6)/1 perchloric acid standard solution with mass fraction of potassium dichromate. 15.4 Spectral filter, nominal value is 10%, 20%, 30% (4%) 15.5 Standard absorption laser: specification error is 0.2% for 10.0m+, 15.6 Stray radiance standard drop or filter, the cut-off wavelength of the stray radiance filter is not less than 225tm and 365 respectively, the absorbance in the cut-off area is not less than 3, and the transmittance in the optical zone is not less than 0%: pass: the above is approved by the National Technical Supervision Bureau of Beijing and meets the requirements of this regulation. IV. Verification Items and Verification Methods
16 External Regulations According to the requirements of the first article, the instrument is calibrated after preheating. 17 Stability
17.1 When the instrument is operating without receiving light, adjust the instrument quarter point so that the display value is 0%, and observe 3mil, record the maximum change of the transmittance value, which is the dark current stability. 17.2. When the receiving element needs to be illuminated: open the wavelength range of the instrument and adjust the transmittance by 10nm, observe for 3min, and record the maximum change of the transmittance indication, that is, the stability of the photocurrent (when the wavelength is changed, allow 5min for the light to stabilize).
Note: 17 is recorded from the beginning of the instrument. 18. Wavelength accuracy and wavelength stability
18, 1. Use 5 evenly distributed spectrum lines in the spectral line of the lamp as reference, and measure the minimum bandwidth (automatic scanning instrument, scanning speed is about 0m/uit), adjust the slit instrument to a smaller slit such as 0.02D1m, then use the 486.13Lm spectrum line of the hydrogen lamp (the 486.00nm spectrum line of the fluorine lamp), and the tungsten lamp to use the absorption film of about 10nm (subject to the actual calibration) as the reference, and measure again, and measure the above three times in succession.
18.2 Wavelength accuracy is calculated according to formula (2): 5
The measured value of the first wave:
The standard value of the first wave.
Wavelength repeatability is calculated according to formula (3):
JJG 375-
4. - 1/3%,- 2
o- nax,2:1/322.
wash: note the total exchange of the bureau's shape, the fixed view of the weekly food chemical reduction efficiency less source clean city work special stimulation growth. 19 fixed sensitivity
nuclear instrument slow down the length of the world at 20m, 625mm, adjust the midstream to zero, open the light door, the word section I degree, the signal transmission is 10, read the corresponding slit width. The small ice section fan
light book type false instrument with recording lamp 435.83m digital instrument with atmosphere atmosphere) lamp material evaluation line,: 656, "m556.31), there are more than 2 pin width must be recorded, set the instrument center to borrow width, measure the maximum transmission ratio of the spectrum line, the dynamic type only by the short wave long liquid side to ask enough dynamic, automatic scanning phase device directly scan out the picture, record the center wavelength two measured transmission ratio value The difference between the sensitivity readings,, and, when the value is reduced by %, is the actual bandwidth of the instrument. 21. Accuracy of transmittance reduction and repeatability of transmittance
21.1 Use the standard wavelength mentioned in 15.3] 15.5 standard absorption pool: take .(1111:/.peroxy acid as the wavelength, spectral bandwidth 27m (the instrument with detailed bandwidth adjustment is not available), measure the transmittance of the product at 25, 257, 313, 350m respectively, and measure it three times in succession. 212 Use the light filter with the nominal transmittance value of %.20%,%: refer to 21.1 for the remaining conditions, measure the transmittance at 4, 6351m wavelength respectively, with air as the reference, and measure the transmittance in the air for three times. 21.3 Direction (4) to calculate the accuracy of remote transmittance reduction:
4, — 1/32t
wherein is the first transmission value of the test insect;
F.—Transmittance standard value:
According to formula (5), the transmittance repeatability is calculated as:
G, — x [, -1/32; 1
In the formula, take the light ratio of 30% of the nominal value of the reflectance ratio at: 5462m7 wave, 22 benzene line half straightness
The instrument filter length is placed in the starting position, with party 2, the light receiving room is at +0, the intercept and reference back are blank, and the gold station is set to the south, the difference between the starting point and the most human shift of the star map (the recording pen is allowed to move when the light source is formed into a light film)
3.1 The instrument is set at ±]nm, the light width is 2r, the product and the image are all blank, the small egg process is recorded, the direction time is not three 1, the fixed liquid length is 2, and the peak-to-peak difference is recorded on the spectrum, that is, 100% noise
JJG3751996
23.2 The wavelength of the instrument is set at 5001m, and the minimum distance is taken. The light shield is inserted into the sample beam. According to the method of 23.1, the noise is detected
Note: The results of 22 and 23 are based on the type instrument. 24 Power supply voltage effect
The wavelength of the instrument is set at 500mrm, and the voltage regulator is used to input 220V. The transmittance indication is adjusted to 1[: Change the input voltage: record the transmittance indication of the instrument at 198, 242V (the voltage changes during the adjustment at 220V to 100%!).
Liu reverse reflection This pizza is only equipped with (·-100% block, 95% can be used instead of 100% 25 stray roll rate
25.1 The quality of the film is more than 10 sodium iodide standard solution, 10mm standard quartz absorption cell, distilled water as reference, with a light band adjustment device set to the maximum spectral band search (continued to adjust the instrument, under the full height of the shoe tea) at 220m, measure the radiation ratio of the liquid: 25.2 Use the quality of the sub-pin age sodium standard drop liquid of 50k, at 36Unm wavelength, the rest is 24.1, measure the mother solution radiation ratio.
The above Both models can also use stray radiation sheets that meet the requirements of 15.6. 25.3 When it is necessary to measure the low forbidden radiation rate value of the instrument, use an attenuation sheet with a transmittance of 0.1%. First observe the transmittance value of the attenuation sheet, and use the attenuation sheet as a reference to measure the reflectance value of the standard material. The product of the two transmittance values is the frame scattered radiation rate.
26 Absorption cell matching
The same light quartz absorption cell attached to the instrument is filled with 220.700m of distilled water; the glass absorption cell is filled with potassium chromate solution containing 30mg of chromate, and at 440nm, 700m of distilled water is added. m, adjust the transmittance ratio of one cell to 100%, and measure the transmittance ratio of other filters. For the detector with a transmittance range of only 100%, 95% can be used instead of 100%: 27 Insulation resistance
Use a 5V megohmmeter to measure the insulation resistance between the power input terminal and the housing (or auxiliary terminal) of the detector (during the test, the instrument is in a non-working state).
28 For other types of single-beam UV-visible spectrophotometers whose measurement methods are completely included in the scope of this specification, the main technical indicators can be verified with reference to the above verification method, and the technical requirements can be verified according to the verification method specified in the instrument. The index requirements are specified.
V. Processing of calibration results and calibration cycle
29 Newly manufactured instruments should be fully inspected according to 16.-1 and 21-27, and must be inspected according to 20; instruments that have been used or repaired do not need to undergo the calibration listed in 20, 24, and 27, but must be calibrated when necessary. 30 For instruments that have passed the calibration according to this procedure, a calibration certificate will be issued, and the category will be indicated. If the indicators in the required inspection items (excluding 2G) do not meet the requirements of Class A, it will be classified as Class I use. If it does not meet the requirements of Class 1, it will be judged as unqualified, and a calibration result notice will be issued, indicating the test data. J.1G 3751996
31: The calibration cycle is 1 year. During this period, if the instrument has been repaired or there is doubt about the measurement results, it should be inspected in time.The peroxy acid is used as the multi-bandwidth, the spectral bandwidth is 27m (the instrument with bandwidth adjustment is not available), the transmittance of the product is measured at 25, 257, 313, 350m, and the measurement is repeated three times. The nominal transmittance is %.20%, and the light filter is generated in the wavelength range of 21.2: the rest of the conditions refer to 21.1, and the transmittance is measured at 4, 6351m, 6351m, and 6351m respectively, with air as the reference. The transmittance of the air is measured three times in a row according to 21.3. The transmittance is calculated by formula (4):
4, — 1/32t
wherein the transmittance is the measured value for the first time;
F.—Transmittance standard value:
According to formula (5), the transmittance repeatability is calculated as:
G, — x [, -1/32; 1
Wherein, 2Take the light ratio of 30% of the nominal value of the reflection ratio at: 5462m7 wave, 22 benzene line half straightness
Instrument filter length is placed in the starting position, with party 2, the light receiving room is at +0, the intercept and reference back blank, and the gold station is set to the south, the difference between the starting point of the star map and the most human shift (the recorder is allowed to move when the light source is formed into a light sheet)
3.1 Instrument method is set at ±]nm, the light width is 2r , the items and the objects are all blank, the government records the small egg process, the time is not three 1, the liquid is fixed for 2 certificates, the medical spectrum is recorded on a group of peaks and the final difference is recorded, that is, the road will be 100% called sound
JJG3751996
23.2 The instrument wavelength is set at 5001m, the minimum disk range is taken, the light baffle is inserted into the sample beam, and the method in Article 23.1 is used to detect % noise Lu
Note: The results of Articles 22 and 23 are self-reflective instruments, etc. 24 Power supply voltage E influence
Instrument wavelength is set at 500mrm, input 220V voltage with voltage regulator, adjust transmittance indication to 1 [: change input voltage E: record the transmittance indication of the instrument at 198, 242V (voltage changes during the 220V position to re-adjust to 100%!
Liu reverse reflection This pizza is only (·-100% block of the device, 95% can be used instead of 100% 25 stray roll rate
25.1 film quality is more than 10 sodium iodide standard solution, 10mm standard quartz absorption cell, distilled water as reference, with a light orange band adjustment block of the simulator set to the most people spectral band search (continued case adjustment instrument, under the shoe full height teaware ) At 220m, measure the radiation ratio of the liquid: 25.2 Use a standard sodium standard liquid with a mass of 50k, at a wavelength of 36Unm, and the rest of the time according to 24.1, measure the radiation ratio of the mother solution.
The above two items can also use the stray box rate data sheet that meets the requirements of 15.6. 25.3 When it is necessary to measure the low forbidden radiation rate value of the instrument, use an attenuation sheet with a transmittance of 0.1%, first observe the transmittance value of the attenuation sheet, and use the attenuation sheet as a reference to measure the radiation ratio of the mother standard material. The product of the two transmittance values is the frame radiation ratio.
26 Absorption cell matching
The same light quartz absorption cell attached to the instrument is filled with distilled water 220.700mm; the glass absorption cell is filled with potassium chromate solution containing 30mg of chromate, at 440nm, add distilled water at 700mm, adjust the transmittance ratio of one cell to 100%, and measure the transmittance ratio of other filters. For the detector with a transmittance range of only 100%, 95% can be used instead of 100%: 27 Insulation resistance
Use a 5V megohmmeter to measure the insulation resistance between the power input terminal of the detector and the casing (or auxiliary terminal) (during the test, the instrument is in a non-working state).
28 For other types of single-beam UV-visible spectrophotometers whose measurement methods are fully included in the scope of this specification, the main technical indicators can be found in Calibration shall be carried out according to the above calibration method. The technical requirements may be in accordance with the index requirements specified in the instrument specification.
V. Processing of calibration results and calibration cycle
29 Newly manufactured instruments shall be fully inspected according to 16.-1 and 21-27, and according to 20 when necessary; instruments after use and repair may not be calibrated according to 20, 24, and 27, but shall be calibrated when necessary. 30 For instruments that have passed the calibration according to this procedure, a calibration certificate shall be issued, and the category shall be indicated. If the indicators in the required inspection items (excluding 2G) do not meet the requirements of Class A, it shall be classified as Class I use; if it does not meet the requirements of Class 1, it shall be judged as unqualified, and a calibration result notice shall be issued, and the test data shall be indicated. J.1G 3751996
31: The calibration cycle is 1 year. During this period, if the instrument has been repaired or there is doubt about the measurement results, it should be inspected in time.The peroxy acid is used as the multi-bandwidth, the spectral bandwidth is 27m (the instrument with bandwidth adjustment is not available), the transmittance of the product is measured at 25, 257, 313, 350m, and the measurement is repeated three times. The nominal transmittance is %.20%, and the light filter is generated in the wavelength range of 21.2: the rest of the conditions refer to 21.1, and the transmittance is measured at 4, 6351m, 6351m, and 6351m respectively, with air as the reference. The transmittance of the air is measured three times in a row according to 21.3. The transmittance is calculated by formula (4):
4, — 1/32t
wherein the transmittance is the measured value for the first time;
F.—Transmittance standard value:
According to formula (5), the transmittance repeatability is calculated as:
G, — x [, -1/32; 1
Wherein, 2Take the light ratio of 30% of the nominal value of the reflection ratio at: 5462m7 wave, 22 benzene line half straightness
Instrument filter length is placed in the starting position, with party 2, the light receiving room is at +0, the intercept and reference back blank, and the gold station is set to the south, the difference between the starting point of the star map and the most human shift (the recorder is allowed to move when the light source is formed into a light sheet)
3.1 Instrument method is set at ±]nm, the light width is 2r , the items and the objects are all blank, the government records the small egg process, the time is not three 1, the liquid is fixed for 2 certificates, the medical spectrum is recorded on a group of peaks and the final difference is recorded, that is, the road will be 100% called sound
JJG3751996
23.2 The instrument wavelength is set at 5001m, the minimum disk range is taken, the light baffle is inserted into the sample beam, and the method in Article 23.1 is used to detect % noise Lu
Note: The results of Articles 22 and 23 are self-reflective instruments, etc. 24 Power supply voltage E influence
Instrument wavelength is set at 500mrm, input 220V voltage with voltage regulator, adjust transmittance indication to 1 [: change input voltage E: record the transmittance indication of the instrument at 198, 242V (voltage changes during the 220V position to re-adjust to 100%!
Liu reverse reflection This pizza is only (·-100% block of the device, 95% can be used instead of 100% 25 stray roll rate
25.1 film quality is more than 10 sodium iodide standard solution, 10mm standard quartz absorption cell, distilled water as reference, with a light orange band adjustment block of the simulator set to the most people spectral band search (continued case adjustment instrument, under the shoe full height teaware ) At 220m, measure the radiation ratio of the liquid: 25.2 Use a standard sodium standard liquid with a mass of 50k, at a wavelength of 36Unm, and the rest of the time according to 24.1, measure the radiation ratio of the mother solution.
The above two items can also use the stray box rate data sheet that meets the requirements of 15.6. 25.3 When it is necessary to measure the low forbidden radiation rate value of the instrument, use an attenuation sheet with a transmittance of 0.1%, first observe the transmittance value of the attenuation sheet, and use the attenuation sheet as a reference to measure the radiation ratio of the mother standard material. The product of the two transmittance values is the frame radiation ratio. www.bzxz.net
26 Absorption cell matching
The same light quartz absorption cell attached to the instrument is filled with distilled water 220.700mm; the glass absorption cell is filled with potassium chromate solution containing 30mg of chromate, at 440nm, add distilled water at 700mm, adjust the transmittance ratio of one cell to 100%, and measure the transmittance ratio of other filters. For the detector with a transmittance range of only 100%, 95% can be used instead of 100%: 27 Insulation resistance
Use a 5V megohmmeter to measure the insulation resistance between the power input terminal of the detector and the casing (or auxiliary terminal) (during the test, the instrument is in a non-working state).
28 For other types of single-beam UV-visible spectrophotometers whose measurement methods are fully included in the scope of this specification, the main technical indicators can be found in Calibration shall be carried out according to the above calibration method. The technical requirements may be in accordance with the index requirements specified in the instrument specification.
V. Processing of calibration results and calibration cycle
29 Newly manufactured instruments shall be fully inspected according to 16.-1 and 21-27, and according to 20 when necessary; instruments after use and repair may not be calibrated according to 20, 24, and 27, but shall be calibrated when necessary. 30 For instruments that have passed the calibration according to this procedure, a calibration certificate shall be issued, and the category shall be indicated. If the indicators in the required inspection items (excluding 2G) do not meet the requirements of Class A, it shall be classified as Class I use; if it does not meet the requirements of Class 1, it shall be judged as unqualified, and a calibration result notice shall be issued, and the test data shall be indicated. J.1G 3751996
31: The calibration cycle is 1 year. During this period, if the instrument has been repaired or there is doubt about the measurement results, it should be inspected in time.
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