This part specifies the methods and basic requirements for field efficacy plot tests of fungicides for the control of citrus scab (Sphaceloma fawcettii). This part applies to field efficacy plot tests and efficacy evaluation of fungicides for the control of citrus scab for registration. GB/T 17980.102-2004 Guidelines for field efficacy tests of pesticides (II) Part 102: Efficacy tests of fungicides for the control of citrus scab GB/T17980.102-2004 Standard download decompression password: www.bzxz.net
This part specifies the methods and basic requirements for field efficacy plot tests of fungicides for the control of citrus scab (Sphaceloma fawcettii). This part applies to field efficacy plot tests and efficacy evaluation of fungicides for the control of citrus scab for registration.

GR/T1798G.1022CC4
In vitro efficacy test is one of the contents of pesticide management and an important technical basis for formulating agricultural product labels. Labels are the only way to ensure safe and reasonable use of pesticides. In order to standardize the content of pesticide test methods, make the test more scientific and unified, and keep pace with international standards, so that my country's in vitro efficacy test has international recognition, we have formulated the national standards for in vitro efficacy test. This series of standards includes the European and Mediterranean Plant Protection Organization (EPPOIH) pesticide test guidelines and the United Nations Food and Agriculture Organization (FAO) Asia-Pacific region guidelines, and is formulated based on my country's actual situation after large-scale in vitro efficacy test tests. It is necessary to use the first-line pesticide system for prevention and control, in order to determine the most suitable dosage for prevention and control of the disease, test the effect of the pesticide on fruit trees and non-target beneficial organisms, and evaluate the efficacy and safety of the registered fungicide. Because the technical data for combined use are not available, this part of GB/T 17989 is specially formulated. This part is one of a series of standards, but it is an independent part issued by the Ministry of Agriculture of the People's Republic of China, and most of the units involved are the Institute of Pesticide Inspection of the Ministry of Agriculture. The main drafters of this part are: Wu Xinban, Bai Wenxing, Liu Naimei, Gu Baogen, Liu Qi, Xiao Hui, and Lu Weihai. The Institute of Pesticide Inspection of the Ministry of Agriculture is responsible for interpretation. 1
■ Standard National
Guidelines for In-house Efficacy Tests (II)
Part 102: Fungicides for Control of Citrus Scabies (/ 17930.102-702 This section stipulates the methods and basic requirements for inter-plot efficacy trials of fungicides for the treatment of citrus diseases. This section applies to inter-plot efficacy trials of fungicides for the treatment of citrus diseases. The evaluation of the efficacy of fungicides is as follows: 2. Test items 2.1 Selection of test objects, crops and varieties The test objects are citrus scab, the test crops are trees, sensitive varieties are selected, the variety name and tree age are recorded. 2.2 Environmental conditions The inter-plot trials should be carried out in the year when the management is similar or the subject fertilizer is applied. The cultivation conditions of all test plots (soil type, fertilizer application, plant age, row spacing, etc.) should be consistent and in line with local agricultural practices (G 3.1.1 The test drug shall be clearly identified by its name or code, generic name, prototype and manufacturer. The test drug shall be treated with no less than three doses specified in the plan or the agreement (the agreement signed between the test entrusting party and the test contractor). 3.1.2.2.3.4.5.6.7.7.8.9.10.11.1.2.2.3.4.6.8.9.11.2.2.3.4.7.8.9.12.13.2.2.3.4.7.8.9.13.13.2.2.3.4.7.8.9.13.13.2.2.3.4.7.8.9.13.13.2.2.3.4.7.8.9.13.13.2.2.3.4.7.8.9.13.13.2.2.3.4.7.8.9.13.13.2.2.3.4.7.8.9.13.13.13.2.2.3.4 ... 3.2.2 Plot area and weight
Plot area: 200-3000m2 of mature fruit trees.
Repetition rate: no less than one repetition.
3.3 Application method
3.3.1 Usage method
Follow the instructions and label instructions: Application should be in accordance with local scientific agricultural practices. 3.3.2 Type of equipment used
Select the equipment used in production, record the type of equipment used and all the information of the working conditions (non-strenuous work, manual), and record the dosage deviation of ±10%.
3.3.2 Time and frequency of application of pesticides
According to the requirements of the equipment and the label instructions, the application of pesticides is generally carried out from March to May. For the duration of application of pesticides and the duration of the effect of the pesticide, the maximum is two days. 3.3.4 Use dosage and volume
According to the dosage requirements and the dosage specified in the label, the dosage of the active ingredients of the drug is usually expressed as ng/kg or g/plant, and the dosage and the dosage of the liquid used per tree are recorded (1/hm2). 3.3.5 Data requirements for drugs for controlling other diseases and insect pests If other drugs are to be used, drugs that can be tested and have no effect on the test should be selected, and all small operations should be carried out in the same place, and they should be used separately with the test drugs and control drugs to control the positive interference of these drugs in the small process, and record the data used for such drugs.
4 Inspection, recording and measurement methods
4. 1 Meteorological and soil data
4.1.1 Meteorological data
During the test, rainfall (rainfall type and total rainfall, expressed in mr.) and temperature (daily average high and low temperatures, expressed in mr.) data should be obtained from the test site or the nearest meteorological station. In special conditions, additional data should be collected. The adverse climatic factors that affect the test results during the entire test period, such as severe weather and long-term droughts, rains, etc., should be recorded. 4.1.2 Soil data
Record soil type, temperature, and humidity. H. Organic matter content, moisture (dry, micro or labor), soil cover (effective crops, difficult to grow, weeds) and other information, 4.2 Survey method, time start number
4.2.1 Survey method
Investigate two plants in each cell, and each forest junction east, west, south, north and middle hill points: annual point adjustment, two sales of all leaves and 6 fruit leaves. Grading method:
(Grade: disease-free:
1 fluff, 1 to 5 per diseased rice,
3 destruction; 5 diseased leaves: ~10
level 5; 11-1 diseased spots on each leaf,
level 7; 16-20 diseased spots on each leaf;
level 1, 1 diseased spot on each leaf. Www.bzxZ.net
fruit grading method,
(level: none:
level 1; diseased spots are taken from the entire fruit area: level 5: the diseased spots are connected and account for 6% to 10% of the entire fruit area; level 7: diseased spots are connected and account for 11%-25% of the fruit area. Accurately identify 26%-50% of the fruit surface area: Level 9: The lesions are connected on two pieces of the entire fruit area more than 51%: 4.2.2 Investigation time and frequency
Investigation of disease base before application The last time is about 1-2℃ day after the final confirmation of the violation, or according to the requirements of the agreement, 4.2.3 Efficacy calculation method
Disease index = 2 each spit (the whole fruit is generally 1C5..
Total leaves (fruits) are returned for inspection and entered 9
Control effect (%)- (1-equal amount of drug before drug treatment index treatment area drug treatment data observation) × 1GG (2) blank control area disease index after drug treatment × treatment area disease index before drug treatment! Or both treatment effect [, no basic effect before drug application (for 》 = blank control medical tumor stimulation grape number need medical disease index × 1GG ... (3) blank control area disease index
4.3 Other effects on crops
GD/T 17953.102-20J4
Observe whether the crops have drug damage. If there is drug damage, the degree of drug damage should be recorded. In addition, other effects on crops should also be recorded (such as promoting maturity, stimulating growth, etc. 1. Drug records are as follows:) The drug can be measured or calculated, and the value table is not used. b) In other cases, the rate and intensity of the drug can be expressed in two ways: 1) Record the drug damage situation of each plot according to the drug classification method. One, ten, eleven, ten ten ten, one ten ten ten represent one; no drug content;
ten: mild drug damage, no impact on normal growth; ten ten: obvious drug damage, can be recovered, small production 1! : still considered drug damage, affecting normal growth, and causing certain losses to production and quality, "generally require compensation for part of the economic loss:
ten ten ten ten: serious drug damage, growth retardation, strict quantity and dish placement, must compensate for economic losses. 2) Compare the drug-treated area with the blank area, and determine the total percentage of drug damage. At the same time, the drug damage symptoms (image, shape) should be accurately described. And provide real photos, pregnancy images, etc. 4.4 Impact on other organisms
4.4.1 Impact on other organisms
Any signs of other harmful effects should be recorded, including beneficial and negative effects: 4.4.2 Effects on other non-target organisms
Record the effects of the pesticide on endogenous organisms and beneficial insects in the test area. 4.5 Product production and quality
It is necessary to record:
5 Results
The results obtained from the trial should be analyzed by biostatistics (using the [MRT method]), and a results report should be written in a formal format, and the test results should be analyzed. The original data should be kept for record verification
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