This standard specifies the determination method of sodium propionate and calcium propionate in soy sauce, vinegar, bread and cakes. This standard is applicable to the determination of propionate in soy sauce, vinegar, bread and cakes. The detection limit of this method is 0.05k/kg for bread and cakes, and 0.02g/kg for soy sauce and vinegar. GB/T 5009.120-2003 Determination of sodium propionate and calcium propionate in food GB/T5009.120-2003 Standard download decompression password: www.bzxz.net

ICS67.040
National Standard of the People's Republic of China
GB/T 5009.120-2003www.bzxz.net
Substitute GB/T14940--1994
Determination of sodium propionate and calcium propionate in food
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2003-08-11 Issued
Ministry of Health of the People's Republic of China
China National Standardization Administration
2004-01-01
B/T5009.120—2003
This standard replaces GB/T11C—1994 Micro-determination method for domestic sale and domestic brand in foods 31994. The main modifications are as follows
This standard is different from GB/T14940
The Chinese name of the standard has been changed to [Determination of propionic acid and calcium propionic acid in foods] ：According to: B/T20WU1,4-.2(11 standard writing rules.
This standard is proposed by the Ministry of Health of the People's Republic of China. Part 1: Chemical analysis method. The structure of the original standard is revised. The drafting units of this standard are: Food Health Supervision and Inspection Institute of the Ministry of Health, Wuhan Municipal Health and Epidemic Prevention Station, Beidian Municipal Health and Epidemic Prevention Station. The drafters of the standard are: Wang Zhutian, Song Fengying, Gao Heqi, Guan Fengwu, Guan Hehua. The original standard was published in 1994. This standard is the first revision and was first issued in 2003. E
1 Scope
This standard specifies the determination of sodium bicarbonate and calcium bicarbonate in foods. This standard specifies the determination of sodium bicarbonate in soy sauce, esters, bread and cakes. This standard is applicable to the determination of propionaldehyde in oil, vinegar, bread and cakes. The detection limit of this method is 15g/kg for bread and cakes: 15g/kg for soy sauce, 10g/kg for cakes. 2 Principle
GB/:6009.120—2003
After the sample is deionized, the acid is converted into propionaldehyde, which is then passed through a water vaporizer and then connected to a phase chromatography + hydrogen ionization instrument for detection. The result is compared with the standard.
3.1 Take 1% phosphoric acid from phosphoric acid solution. (%) add water to 1ml.3.2 Acid drop: take 1ml. 0950mL.
3.3 Silicon pool:
3.4 ​​Acrylic acid standard solution standard spectrum filter (10mg/mL) accurately weigh 250mL of acid dry ml. Filter, add water to the scale. The seat is full, and the equipment is made of water! /~2/ standard carving series 4 according to the instrument
4.1 Gas plate chromatograph: with chlorine flame ionizer (FI1). 4.2 Water gas combustion equipment,
5 Analysis steps
5.1 Root report
Accurately weigh 3 pre-mixed and precipitated samples (drawing package, point or sample needs to be air-dried at room temperature, grind), bone 500m. Steam Rinse with 50ml water for 1 week and transfer to the steamer. Put 250ml of filter oil in the ice cube tray as the collection liquid. When the steam is about 250ml, take it out and place it at room temperature for 10 minutes. Drain to the mark and pipette 11mL of sensitive liquid into the test tube and add 0.ml formic acid. According to the color supply order. 2 Chromatographic components: 3m inner diameter, 1 month Porpak QS, instrument performance: column temperature 130℃, injection port, exchange speed 220℃ gas sieve system: standard gas 50mL/min gas 5mL/min gas: 50ml /min
5.3 Determination
Take various concentrations of standard solution in the standard series, add 5ml of A, mix, take, and inject into gas chromatography to obtain the peak quotient of different concentrations of acid. According to the concentration and peak height, prepare the standard curve. At the same time, inject the sample, and compare the peak height of the sample with the standard curve for quantification.
GB/T5009.120—2003
5.4 Calculation of results
Calculate according to the following formula:
In the formula:
Propionic acid content in the same solution, unit is gram per dry gram (s/kg)A
Propionic acid content in the solution to be determined, unit is microgram calorimeter (L) Sample mass unit is gram (g).
Sodium bisulfite content = propionic acid content × t.256. Propionic acid content × 1.2569.
Propanediol content
The calculated result shall be within two significant figures, and the absolute difference of the instantaneous independent measurement results obtained under repeatability conditions shall not exceed 10% of the arithmetic half mean.
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