Pesticide guidelines for the field efficacy trials—Part 28:Insectides against broad-leaved tree longicorn beetle
Some standard content:
ICS 65.100
Agricultural Industry Standard of the People's Republic of China
NY/T1464.28--2010
Pesticide guidelines for the field efficacy trialsPart z8: Insecticides against broad-leaved tree longicorn beetle2010-05-20 issued
2010-09-01 implementation
The Ministry of Agriculture of the People's Republic of China issued
NY/T1464 "Standards for the Test of Pesticides for Outdoor Efficacy" is a series of standards, including 36 parts: Part 1: Cides for the control of locusts; Part 2: Insecticides for the control of rice water bugs; Part 3: Cides for the control of cotton stink bugs; Part 4: Insecticides for the control of pear yellow powder; Part 5: Insecticides for the control of apple aphids; Part 6: Insecticides for the control of vegetable thistles; Part 7: Fungicides for the control of tobacco Anthracnose of grass: Part 8: Fungicides for the control of tomato virus diseases; Part 9: Fungicides for the control of pepper virus diseases; Part 10: Fungicides for the control of mushroom wet bubble disease; Part 11: Fungicides for the control of banana black spot; Part 12: Fungicides for the control of grape powdery mildew; Part 13: Fungicides for the control of grape anthracnose; Part 14: Fungicides for the control of water spores; Part 15: Fungicides for the control of wheat fusarium head blight; Part 16: Fungicides for the control of wheat root rot; Part 17: Herbicides for the control of weeds in mung bean fields; Part 18: Herbicides for the control of weeds in the sesame order; Part 19 : Herbicides for controlling weeds in wolfberry fields: - Part 20: Herbicides for controlling weeds in tomato fields: - Part 21: Herbicides for controlling weeds in cucumber fields; - Part 22: Herbicides for controlling weeds in garlic fields; - Part 23: Herbicides for controlling weeds in hunting fields; - Part 24: Herbicides for controlling red peduncle weeds; - Part 25: Herbicides for controlling tea weeds in tobacco beds; - Part 26: Test for cotton adhesion promoters; - Part 27: Herbicides for controlling aphids in cruciferous vegetables; - Part 28: Herbicides for controlling longhorn beetles in loblolly trees - Part 29: Insecticides for controlling brown pine beetles; - Part 30: Fungicides for controlling cold tobacco hornworms Diseases: Part 31: Fungicides for the control of ginger blight; Part 32: Fungicides for the control of tomato bacterial wilt; Part 33: Fungicides for the control of bean rust; Part 34: Fungicides for the control of eggplant verticillium wilt; Part 35: Herbicides for the control of old weeds in direct-seeded vegetables; Part 36: Herbicides for the control of weeds in spinach fields. This part is Part 28 of the "Guidelines for the Four-room Efficacy Test of Pesticides": NY/T1464.28—2010
NY/T1464.28—2010
This part is proposed and managed by the Crop Production Management Department of the Ministry of Agriculture of the People's Republic of China. The drafting unit of this part is the Pesticide Control Institute of the Ministry of Agriculture. The main drafters of the wood section: Ji Lixin, Zhang Tiandong, Zhu Rui, Chen Jingyuan, Zhang Wenjun, Tao Lingmei, 1 Scope
Guidelines for field efficacy tests of pesticides
Part 28: Pesticides for controlling broadleaved tree beetles NY/T1464.28—2010
This part specifies the methods and requirements for field efficacy tests of pesticides for controlling broadleaved tree beetles [including mulberry beetles (Aprion agermuri), Arwlo-phoraglahripennis, Batocera harsfieldi, Aprion sreuinsoni, etc. of the Cerambycidae family].
This part is applicable to the field efficacy tests and efficacy evaluation of pesticides for controlling broadleaved tree beetles. Field efficacy tests for other pests of the Cerambycidae family can be used as a reference. 2 Test conditions
2.1 Test objects and crops
The test objects are mulberry beetle, glabripennis beetle, clouded beetle, rusty grain beetle, etc. of the Cerambycidae family, and the test crops are the main local tree species that are sensitive to longhorn beetles (such as poplar, mulberry, fig, willow, ash, Sophora japonica, Robinia pseudoacacia, citrus cabinet, etc.): record the name of the tree species.
2.2 Environmental conditions
Select representative forest land (forest, forest belt, street tree) where forest water longhorn beetles have occurred seriously in previous years, and generally require the trees to grow normally. The main trunk, branches, main shoots and branches of target pests are used as test objects. 3. Experimental design and arrangement
3.1 Experimental treatment
3.1.1 Pesticide
3.1.1.1 Experimental pesticide
The experimental pesticide treatment shall be no less than 3 doses or the dosage specified in the agreement (the experimental agreement signed by the test entrusting party and the test requesting party). Record the generic name (in Chinese or English) or code of the pesticide, dosage form, content, manufacturer and treatment agent (expressed in effective concentration mg/L or mg/L).
3.1.1.2 Control pesticide
The control pesticide shall be a registered pesticide that has been proven to be effective in practice, and its type and mode of action shall be similar to that of the experimental pesticide. The control pesticide shall be used at the local conventional application rate. Special circumstances may depend on the purpose of the test. If the test agent is a single agent, at least another local commonly used single agent shall be used as a control agent; if the test agent is a mixture, each single agent and the local commonly used agent shall be used as a control agent.
Record the generic name, dosage, content, manufacturer, and application amount of the control agent. 3.1.2 Blank control
Set the special agent treatment as a blank control:
3.2 Number of repetitions
Each treatment shall be repeated at least 4 times.
3.3 Plot arrangement
3.3.1 Plot area
NY/T 1464.28—2010
When the control targets are larvae and eggs: select 3 trees with a distance of 1.5m and set up protection rows. When the control targets are adults, select 5 or 7 saplings 1.5m apart as standard plants, one of which is a blank control with clean water, and the others are used for pesticide treatment. Use a wire mesh cage with a length of 0.6m, a width of 0.6m and a height of 1.5m to cover the saplings or wood sections and branches to prevent the escape of adult longhorn beetles. 3.3.2 Plot arrangement
Each plot is arranged in random blocks, and the plot arrangement diagram is recorded. 3.4 Requirements for pesticides used to control pests other than the test object If other pesticides are needed to control diseases, insects, and pests other than the test object during the test, pesticides that have no effect on the test pesticide and the test object should be selected, and they must be used separately from the test pesticide and the control pesticide. All test plots should be treated evenly. Record the data of the application of such pesticides (pesticide name, content, dosage form, manufacturer, application dosage, application method and application time): 4 Application
4.1 Application equipment
Use commonly used equipment for application, or select equipment according to the requirements of the agreement. Record all information on the type of equipment used and operating conditions (spray height, nozzle diameter and single plant variable dosage, etc.). Application should ensure that the dosage is accurate and evenly distributed. If the dosage has a deviation of more than 10%, it should be recorded.
4.2 Application method
Spray the test trees one by one, or according to the requirements of the agreement and label instructions. Test for prevention and control of adult beetles: release the adults after applying the pesticide to the standard plant, and cover it with wire mesh, remove the ground cover or lay plastic film for use in the prevention and control effect investigation. 4.3 Application time
Apply the pesticide according to the agreement requirements or label instructions during the appropriate period for dog and cattle control. Record the date of application, the growth period of the tree and the occurrence period of longhorn beetles when applying the pesticide.
4.4 Number of applications
Carry out according to the requirements of the agreement, record the amount of pesticide applied each time and the period of application. 4.5 Application amount
Determine the application amount according to the application method, tree type, growth period, and plot, or carry out according to the requirements of the agreement. Record the application amount, expressed as effective concentration (mg/kg).
5 Investigation
5.1 Investigation of control effect
5.1.1 Investigation method
Eggs: Investigate 3-5 plants in each plot, mark the grooves where eggs are laid, check whether there is fresh feces at the original mark, and peel the bark of the groove with a knife (the eggs are born between the wood and phloem, about 5mm from the top of the groove), and check the survival of eggs: Young: Investigate 3-5 plants in each plot, mark the holes where fresh feces are discharged, and check whether there is new feces at the original mark. No fresh feces means that the young have died. After the completion of the industry, investigate the number of live insects on the plants buried in the cage. 5.1.2 Investigation time and frequency
Before applying the pesticide, investigate the base number of insects. After applying the pesticide, investigate the number of survivors at 3d, 5d, 10d, and 15d respectively. According to the test requirements and the characteristics of the pesticide, the number of investigations can be increased or the investigation time can be extended. 5.2 Investigation of direct impact on crops
Observe whether the pesticide has any effect on the trees. If any damage occurs, record the symptoms, type and severity of the damage. In addition, the beneficial effects on forestry (such as rapid maturity, increased vitality, etc.) should also be recorded. Record the damage in the following ways:
NY/T 1464.28-—2010wwW.bzxz.Net
a) If the damage can be counted or measured, it should be expressed in absolute values, b)
In other cases, the degree and frequency of damage can be estimated by the following two methods, and the symptoms of damage to the trees (dwarfing, chlorosis, jagged shape, etc.) should be accurately described, and physical photos, videos, etc. should be provided. 1) According to the pesticide damage classification method, record the pesticide damage situation in each plot, with -, +, \+, "++,!-→+" indicating: ·: no pesticide, etc.;
-: mild pesticide damage, with visible damage symptoms, which does not affect the normal growth of the tree; ++: obvious pesticide damage, which can be recovered and will not cause a reduction in tree production; ++: severe pesticide damage, which affects the normal growth of the tree and causes a certain degree of loss in tree production and quality; ++: severe pesticide damage, which hinders tree growth and causes serious losses in tree production and quality: economic losses must be compensated. 2) Compare the pesticide-treated area with the blank control area to evaluate the percentage of pesticide damage. 5.3 Investigation of the impact on other organisms
5.3.1 Impact on other diseases and insect pests
Any response to other diseases and insect pests should be recorded. 5.3.2 Impact on other non-target organisms
Record the impact of the pesticide on other non-target organisms and The influence of the weather, 5.4 Other data
5.4.1 Meteorological data
Xie checked and recorded the meteorological data of the test site during the entire test period, including rainfall (rainfall type and daily rainfall, expressed in mm), temperature (average temperature, maximum and minimum temperature, expressed in H) and humidity (maximum and minimum humidity, expressed in %), etc.: 5.4.2 Soil data
Record soil type, soil fertility, tree dust growth, soil cover such as trees or weeds, etc. 5.4.3 Forest phase data
Record altitude, slope, canopy density, forest age, tree height, weak diameter and forest density, etc. 6 Calculation formula and data analysis
The control effect is calculated according to formula (1):
Wuzhong:
PT—control effect, %,
CKXT)
CKIX PT
Po is the number of live insects in the treatment area before application of pesticides, in units of heads; PT, is the number of live insects in the treatment area after application of pesticides, in units of heads; the number of live insects in the blank control area before application of pesticides, in units of heads; CK
is the number of live insects in the blank control area after application of pesticides, in units of heads. The calculation results are retained to two decimal places: the results are analyzed by biostatistical methods (DMRT method). 7 Evaluation and report writing of pesticides
Analyze and evaluate the pesticides according to the results, and issue a formal test report, listing the original data. (1)
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