This standard provides the main morphological and structural characteristics, growth and reproduction, genetic characteristics, and detection methods of grass carp (Ctenopharyngodon idellus). This standard is applicable to the identification of grass carp germplasm. GB 17715-1999 Grass carp GB17715-1999 Standard download decompression password: www.bzxz.net

GB17715 ---1999
This standard is formulated to identify grass carp, protect and preserve the excellent traits and germplasm of grass carp original species, avoid germplasm mixing and degradation during seed production, and carry out effective monitoring of grass carp germplasm. This standard is mainly the result of the "Eighth Five-Year Plan" scientific and technological research, and absorbs the results of the "Sixth Five-Year Plan" and "Seventh Five-Year Plan" scientific and technological research and related achievements of predecessors. Appendix A of this standard is a prompt appendix.
This standard is proposed by the Ministry of Agriculture of the People's Republic of China. This standard is under the jurisdiction of the Freshwater Aquaculture Sub-Technical Committee of the National Technical Committee for Aquatic Standardization. The drafting units of this standard are: Shanghai Fisheries University, Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences. The main drafters of this standard are: Li Sifa, Zhao Jinliang, Cai Wanqi, Zhou Biyun, Lv Guoqing, Fan Zhaoting, Xu Zhongfa. This standard shall be implemented from July 1, 1999. 77
1 Scope
National Standard of the People's Republic of China
Grass carp
GB 17715—1999
This standard gives the main morphological and structural characteristics, growth and reproduction, genetic characteristics, and detection methods of grass carp (Ctenopharyngodon idellus).
This standard is applicable to the identification of grass carp germplasm. 2 Referenced standards
The provisions contained in the following standards constitute the provisions of this standard through reference in this standard. When this standard is published, the versions shown are valid. All standards are subject to revision, and parties using this standard should explore the possibility of using the latest version of the following standards. GB17716—1999 Black carp
3 Name and classification
3.1 Scientific name
Grass carp (Ctenopharyngodon idellus). 3.2 Classification position
Belongs to the order Cypriniformes, the family Cyprinidae, the subfamily Leuciscinae, and the genus Ctenopharyngodon.
Main morphological and structural characteristics
4.1 External morphological characteristics
4.1.1 Appearance
The body is long, slightly rounded in the front, slightly flattened in the back, and the abdomen is round without abdominal ridges. The mouth is terminal and arc-shaped, with the upper jaw slightly protruding from the lower jaw. The snout is slightly blunt and round. The eyes are medium-sized and located below the lateral axis of the body. The scales are large and the lateral line is complete. The gill rakers are short and stick-shaped and sparsely arranged. The caudal fin is deeply forked, with the upper and lower lobes of equal length. The body is grass yellow, the back is blue-gray, the abdomen is silvery white, and the fins are light gray. The external morphology of grass carp is shown in Figure 1.
4.1.2 Countable traits
4.1.2.1 Dorsal fin type: D3.6~7.
4.1.2.2 Anal fin type: A3.7~8.
3 Lateral line scale type: 37 6~47.
4. 1. 2. 3
4.1.2.4 Number of gill rakers on the outer side of the first gill arch: 15~24. 4.1.3 Measurable traits
For individuals with a body length of 19.6~93.4cm and a body weight of 155.0~~12750.0g, the proportion values ​​of the measured traits are shown in Table 1. Approved by the State Administration of Quality and Technical Supervision on April 5, 199978
Implementation on July 1, 1999
Total length/body length
Body length/body height
Body length/head length
GB 17715-1999
Figure 1 Appearance of grass carp
Table 1 Proportion of measured measurable traits
Head length/snout length
Head length/eye diameter
Head length/distance between eyes
1.147±0.0274.262±0.5454.467±0.3953.313±0.4847.371±1.3101.742±0.179
4.2 Internal structural characteristics
Cellular chamber, lip chamber is relatively slender.
4.2.2 Hypopharynx teeth
2 rows, sickle-shaped, with serrations. Tooth formula 2.4～5/4~5.2.4.2.3 Vertebrae
Total number of vertebrae: 39~45.
4.2.4 Peritoneum
Gray-black.
Growth and reproduction
5.1 Growth
Body length/tail peduncle lengthwww.bzxz.net
8.002±1.637
Caudal peduncle length/tail peduncle height
1.138±0.308
The actual values ​​of body length and weight of fish of different age groups are shown in Table 2. The average decalculated values ​​of body length and weight of fish of different age groups corresponding to Table 2, growth equation, and body length and weight relationship are shown in Appendix A (suggested appendix). Table 2 Actual values ​​of body length and weight of fish of different age groups Age!
Measured body length
Measured weight
Measured body length
Measured weight
1) Age is mainly determined by the number of annual rings on the scales. 5.2 Reproduction
3 618~
5.2.1 Sexual maturity age: female fish 4 to 6 years old, male fish 3 to 5 years old. 5.2.2
The gonads of sexually mature individuals mature once a year and spawn once. 4+
4000～
11 000~～
10 500～
12000~
GB 17715—1999
5.2.3 Egg carrying capacity: The egg carrying capacity of individuals in different age groups is shown in Table 3. Table 3 The number of eggs in individuals of different age groups
Age, age
Weight,
Absolute number of eggs, grains
Relative number of eggs, grains/g
6 Genetic characteristics
6.1 Cytogenetic characteristics
6.1.1 Chromosome number
7 916. 7±736. 0
(9. 62±3. 79)X105
122.2±46.8
Somatic cell chromosome number is 2 times, 2n=48.
6.1.2 Karyotype
9 670. 8±1 107. 1
(10.44±4.04)X105
106.8±38.1
13 371.1±1 057. 4
(12.36±4.49)×105
92.7±33.6
13 877. 3±1 195. 1
(14.31±4. 02)X105
103.6±29.5
13 pairs of mid-centromere chromosomes (m group); 7 pairs of sub-centromere chromosomes (sm group); 4 pairs of sub-terminal centromere chromosomes (st group), number of chromosome arms (NF) 88 (see Figure 2). Yes
Figure 2 Karyotype of grass carp
6.2 Biochemical genetic characteristics
6.2.1 Lactate dehydrogenase (LDH) isozyme in muscle 6.2.1.1 The enzyme spectrum of lactate dehydrogenase (LDH) isozyme in muscle is shown in Figure 3. 6.2.1.2 The enzyme band scanning diagram of lactate dehydrogenase (LDH) isozyme in muscle is shown in Figure 4. LDHi
Figure 3 LDH isozyme electrophoresis enzyme spectrum of grass carp muscleFigure 4 LDH isozyme band scanning of grass carp muscleFigure 6.2.1.3 The activity intensity of lactate dehydrogenase (LDH) isozyme bands in muscle is shown in Table 4. Table 4 Activity intensity of LDH isozyme bands in grass carp muscle Enzyme bands
Activity intensity
GB 17715 ---1999
4 Population variation range: The proportion of polymorphic loci in grass carp population is 20.0%~~33.3%, and the average heterozygosity is 0.0454~6.2.1.4
0.1076.
Detection method
7.1 Identification of age
According to the provisions of GB17716.
Determination of fertility
According to the provisions of GB17716.
7.3 Chromosome detection
Perform according to the provisions of GB17716.
Lactate dehydrogenase I.DH) isozyme spectrum analysis 7.4
Perform according to the provisions of GB17716.
GB17715
—1999
Appendix A
(Suggestive Appendix)
Calculated values ​​of body length and weight of grass carp in different age groups, growth equation, body length and weight relationship formula Body length and weight calculated values
The average calculated values ​​of body length and weight of fish in different age groups corresponding to Table 2 are shown in Table A1. Table Al
Average calculated values ​​of body length and weight of fish in different age groups Age·Age
Calculated body length
Calculated weight
Calculated body length
Calculated weight
Growth equation
Female fish:
Male fish:
L, = 113.6[1 — e-0 191 1(e+0.116 5)W, 23 916. 1[1 - e-0. 1911(+0.11 5)]3L 102. 7[1 - e-0. 231 7(α+0 164 5)]W, -16 360. 8[1 - e-0 231 7(+0.164 5)J3In the formula: L. is the body length of fish at -1 age, cmW, — is the body weight of fish at t age, g;
C---natural logarithm;
1——age of fish.
A3 Body length and weight relationship
A3.1 Fish species stage
W = 0. 029 7 × L2. 858 0
W: fish body weight,
L: fish body length, cm.
2 Edible commercial fish stage
W 0. 078 1 X L2. 605 8
A3.3 Broodstock stage
Female fish:
Male fish:
W = 0. 047 1 X L2 76 0
W = 0. 074 7 X L2. 667 1
...(Ai)
...（A2)
（A3)
·（A4）
·（A5)
·（A6)
........................+*..( A7 )..(A8)
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