This standard provides the main morphological and structural characteristics, growth and reproduction, genetic characteristics, and detection methods of grass carp (Ctenopharyngodon idellus). This standard is applicable to the identification of grass carp germplasm. GB 17715-1999 Grass carp GB17715-1999 Standard download decompression password: www.bzxz.net
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GB17715 ---1999 This standard is formulated to identify grass carp, protect and preserve the excellent traits and germplasm of grass carp original species, avoid germplasm mixing and degradation during seed production, and carry out effective monitoring of grass carp germplasm. This standard is mainly the result of the "Eighth Five-Year Plan" scientific and technological research, and absorbs the results of the "Sixth Five-Year Plan" and "Seventh Five-Year Plan" scientific and technological research and related achievements of predecessors. Appendix A of this standard is a prompt appendix. This standard is proposed by the Ministry of Agriculture of the People's Republic of China. This standard is under the jurisdiction of the Freshwater Aquaculture Sub-Technical Committee of the National Technical Committee for Aquatic Standardization. The drafting units of this standard are: Shanghai Fisheries University, Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences. The main drafters of this standard are: Li Sifa, Zhao Jinliang, Cai Wanqi, Zhou Biyun, Lv Guoqing, Fan Zhaoting, Xu Zhongfa. This standard shall be implemented from July 1, 1999. 77 1 Scope National Standard of the People's Republic of China Grass carp GB 17715—1999 This standard gives the main morphological and structural characteristics, growth and reproduction, genetic characteristics, and detection methods of grass carp (Ctenopharyngodon idellus). This standard is applicable to the identification of grass carp germplasm. 2 Referenced standards The provisions contained in the following standards constitute the provisions of this standard through reference in this standard. When this standard is published, the versions shown are valid. All standards are subject to revision, and parties using this standard should explore the possibility of using the latest version of the following standards. GB17716—1999 Black carp 3 Name and classification 3.1 Scientific name Grass carp (Ctenopharyngodon idellus). 3.2 Classification position Belongs to the order Cypriniformes, the family Cyprinidae, the subfamily Leuciscinae, and the genus Ctenopharyngodon. Main morphological and structural characteristics 4.1 External morphological characteristics 4.1.1 Appearance The body is long, slightly rounded in the front, slightly flattened in the back, and the abdomen is round without abdominal ridges. The mouth is terminal and arc-shaped, with the upper jaw slightly protruding from the lower jaw. The snout is slightly blunt and round. The eyes are medium-sized and located below the lateral axis of the body. The scales are large and the lateral line is complete. The gill rakers are short and stick-shaped and sparsely arranged. The caudal fin is deeply forked, with the upper and lower lobes of equal length. The body is grass yellow, the back is blue-gray, the abdomen is silvery white, and the fins are light gray. The external morphology of grass carp is shown in Figure 1. 4.1.2 Countable traits 4.1.2.1 Dorsal fin type: D3.6~7. 4.1.2.2 Anal fin type: A3.7~8. bzxZ.net 3 Lateral line scale type: 37 6~47. 4. 1. 2. 3 4.1.2.4 Number of gill rakers on the outer side of the first gill arch: 15~24. 4.1.3 Measurable traits For individuals with a body length of 19.6~93.4cm and a body weight of 155.0~~12750.0g, the proportion values of the measured traits are shown in Table 1. Approved by the State Administration of Quality and Technical Supervision on April 5, 199978 Implementation on July 1, 1999 Total length/body length Body length/body height Body length/head length GB 17715-1999 Figure 1 Appearance of grass carp Table 1 Proportion of measured measurable traits Head length/snout length Head length/eye diameter Head length/distance between eyes 1.147±0.0274.262±0.5454.467±0.3953.313±0.4847.371±1.3101.742±0.179 4.2 Internal structural characteristics Cellular chamber, lip chamber is relatively slender. 4.2.2 Hypopharynx teeth 2 rows, sickle-shaped, with serrations. Tooth formula 2.4~5/4~5.2.4.2.3 Vertebrae Total number of vertebrae: 39~45. 4.2.4 Peritoneum Gray-black. Growth and reproduction 5.1 Growth Body length/tail peduncle length 8.002±1.637 Caudal peduncle length/tail peduncle height 1.138±0.308 The actual values of body length and weight of fish of different age groups are shown in Table 2. The average decalculated values of body length and weight of fish of different age groups corresponding to Table 2, growth equation, and body length and weight relationship are shown in Appendix A (suggested appendix). Table 2 Actual values of body length and weight of fish of different age groups Age! Measured body length Measured weight Measured body length Measured weight 1) Age is mainly determined by the number of annual rings on the scales. 5.2 Reproduction 3 618~ 5.2.1 Sexual maturity age: female fish 4 to 6 years old, male fish 3 to 5 years old. 5.2.2 The gonads of sexually mature individuals mature once a year and spawn once. 4+ 4000~ 11 000~~ 10 500~ 12000~ GB 17715—1999 5.2.3 Egg carrying capacity: The egg carrying capacity of individuals in different age groups is shown in Table 3. Table 3 The number of eggs in individuals of different age groups Age, age Weight, Absolute number of eggs, grains Relative number of eggs, grains/g 6 Genetic characteristics 6.1 Cytogenetic characteristics 6.1.1 Chromosome number 7 916. 7±736. 0 (9. 62±3. 79)X105 122.2±46.8 Somatic cell chromosome number is 2 times, 2n=48. 6.1.2 Karyotype 9 670. 8±1 107. 1 (10.44±4.04)X105 106.8±38.1 13 371.1±1 057. 4 (12.36±4.49)×105 92.7±33.6 13 877. 3±1 195. 1 (14.31±4. 02)X105 103.6±29.5 13 pairs of mid-centromere chromosomes (m group); 7 pairs of sub-centromere chromosomes (sm group); 4 pairs of sub-terminal centromere chromosomes (st group), number of chromosome arms (NF) 88 (see Figure 2). Yes Figure 2 Karyotype of grass carp 6.2 Biochemical genetic characteristics 6.2.1 Lactate dehydrogenase (LDH) isozyme in muscle 6.2.1.1 The enzyme spectrum of lactate dehydrogenase (LDH) isozyme in muscle is shown in Figure 3. 6.2.1.2 The enzyme band scanning diagram of lactate dehydrogenase (LDH) isozyme in muscle is shown in Figure 4. LDHi Figure 3 LDH isozyme electrophoresis enzyme spectrum of grass carp muscleFigure 4 LDH isozyme band scanning of grass carp muscleFigure 6.2.1.3 The activity intensity of lactate dehydrogenase (LDH) isozyme bands in muscle is shown in Table 4. Table 4 Activity intensity of LDH isozyme bands in grass carp muscle Enzyme bands Activity intensity GB 17715 ---1999 4 Population variation range: The proportion of polymorphic loci in grass carp population is 20.0%~~33.3%, and the average heterozygosity is 0.0454~6.2.1.4 0.1076. Detection method 7.1 Identification of age According to the provisions of GB17716. Determination of fertility According to the provisions of GB17716. 7.3 Chromosome detection Perform according to the provisions of GB17716. Lactate dehydrogenase I.DH) isozyme spectrum analysis 7.4 Perform according to the provisions of GB17716. GB17715 —1999 Appendix A (Suggestive Appendix) Calculated values of body length and weight of grass carp in different age groups, growth equation, body length and weight relationship formula Body length and weight calculated values The average calculated values of body length and weight of fish in different age groups corresponding to Table 2 are shown in Table A1. Table Al Average calculated values of body length and weight of fish in different age groups Age·Age Calculated body length Calculated weight Calculated body length Calculated weight Growth equation Female fish: Male fish: L, = 113.6[1 — e-0 191 1(e+0.116 5)W, 23 916. 1[1 - e-0. 1911(+0.11 5)]3L 102. 7[1 - e-0. 231 7(α+0 164 5)]W, -16 360. 8[1 - e-0 231 7(+0.164 5)J3In the formula: L. is the body length of fish at -1 age, cmW, — is the body weight of fish at t age, g; C---natural logarithm; 1——age of fish. A3 Body length and weight relationship A3.1 Fish species stage W = 0. 029 7 × L2. 858 0 W: fish body weight, L: fish body length, cm. 2 Edible commercial fish stage W 0. 078 1 X L2. 605 8 A3.3 Broodstock stage Female fish: Male fish: W = 0. 047 1 X L2 76 0 W = 0. 074 7 X L2. 667 1 ...(Ai) ...(A2) (A3) ·(A4) ·(A5) ·(A6) ........................+*..( A7 )..(A8) Tip: This standard content only shows part of the intercepted content of the complete standard. 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