This standard specifies the design, field operation, data analysis, fertilizer effect evaluation and report writing of the field test for fertilizer effect identification. This standard is applicable to the field test for fertilizer effect identification. NY/T 497-2002 Technical Specification for Field Test for Fertilizer Effect Identification NY/T497-2002 Standard download decompression password: www.bzxz.net

Agricultural Industry Standard of the People's Republic of China
NY/T 497 -- 2002
Field experiment technology of fertilizer effect testing2002-01-04Promulgated
Implementation on 2002-02-01
Promulgated by the Ministry of Agriculture of the People's Republic of China
NY/T497-2002
Appendix ^ of this standard is a normative appendix, and Appendix B is an informative appendix. This standard was proposed by the Ministry of Agriculture of the People's Republic of China. The drafting units of this standard are: National Agricultural Technology Extension Service Center, Soil and Fertilizer Research Institute of Chinese Academy of Agricultural Sciences, and Huazhong Agricultural University. The main drafters of this standard are: Gao Xiangzhao, Ma Changbao, Yang Fan, Chen Shoulun, Du Sen, Zhu Yu, Xing Wenying, Li Jiakang, and Wang Yunhua. 534
1 Scope
Technical Procedures for Field Tests for Fertilizer Effect Identification NY/T 497--2002
This standard specifies the design of the program, field operation, data analysis, fertilizer effect evaluation and report writing of the field test for fertilizer effect identification. This standard applies to field tests for fertilizer effect identification. 2 Normative References
The clauses in the following documents become the clauses of this standard through reference in this standard. For all dated referenced documents, all subsequent amendments (excluding errata) or revisions are not applicable to this standard. However, the parties to the agreement based on this standard are encouraged to study whether to use the latest versions of these documents. For all undated referenced documents, the latest versions are applicable to this standard. GB/T6274-1997 Terminology of fertilizers and soil conditioners 3 Terms and definitions
The following terms and definitions apply to this standard. 3.1
Compound fertilizer See 2.1.17 of GB/T 6274-1997.
Trace element (micronutrient) trace element; micronutrient See 2.1.25.3 of GB/T6274-1997. 3.3
Secondary element
General name for calcium, magnesium and sulfur.
4 Microbial manure
Microbial fertilizer
Living microbial products made from beneficial microorganisms and playing a major role in improving the nutritional conditions of crops. 3.5
regular fertilizing
Conventional fertilization
Also known as customary fertilization, it refers to the average amount of fertilizer (mainly nitrogen, phosphorus and potassium fertilizers), fertilizer varieties and fertilization methods in the local area in the previous three years. 3.6
Blank control
control
No fertilizer treatment, used to determine the absolute value of fertilizer effect, evaluate soil natural productivity and calculate fertilizer utilization rate. 4 Basic provisions
4.1 Experimental design
4.1.1 Experimental plan
4.7.1.1 Experimental treatment: Design the experimental treatment according to the experimental gate and fertilization method (see Table 1). 535
NY/T 497 -2002
Fertilizer boat method
Table 1 Experimental treatment design
Number of treatments
Compound fertilizer
Not less than
Middle element and trace element fertilizer
Not less than 2
Not less than 2
Experimental treatment
Treatment 1: Test fertilizer
Treatment 2: Conventional fertilization
Treatment 3: Blank control (no fertilizer)Treatment 1: Test fertilizer + conventional fertilization
Treatment 2: Conventional fertilization
Treatment 1: Test fertilizer + conventional fertilization
Treatment 2: Equal amount of fine soil + conventional fertilization
Treatment 1: Test fertilizer + conventional fertilization
Treatment 2: Equal amount of clean water + conventional fertilization
Microbial fertilizer Based on Table 1, in each fertilization method, add a treatment, namely, matrix control (matrix refers to the test sample of active microbial fertilizer, which is sterilized by radiation or medical high pressure and high temperature to meet certain requirements); other fertilizers, according to their functional characteristics, are set separately
4.1.1.2 Experimental repetition: The number of experimental repetitions shall not be less than 4 times. 4.1.2 Experimental method
The field test of two treatments adopts paired design, and the field test of more than two treatments adopts completely randomized block design. The plot area is 20m=~50m. The densely planted crops are smaller, and the cultivated crops are larger. The plot width is not less than 3m for densely planted crops and not less than 4m for cultivated crops. For fruit trees, select plots with small differences in soil fertility and single adult fruit trees of the same age, relatively consistent plant shape and yield for the test, with no less than 6 trees in each plot. The test should select representative soils and no less than 3 test sites. 4.2 Field operations
4.2.1 Test crops and test years
4.2.1.1 Test crops: Select crop varieties suitable for the test fertilizers. 4.2.1.2 Test years: - Two seasons for general crops, and no less than three years for fruit trees. 4.2.2 Test site selection and test preparation
4.2.2.1 Test site selection
The test site should be flat, neat, medium fertility, uniform, and representative. Slopes should be selected with gentle slopes and small differences in fertility; the test site should avoid special plots such as roads and composting sites. 4.2.2.2 Test preparation
Land preparation, setting of protection rows, and division of test areas; irrigation and rowing of plots to avoid cross-irrigation and cross-rowing; analysis of soil nutrient status in the test plot, including organic matter, total nitrogen, available phosphorus, available potassium, pH value, etc., and other items shall be tested according to test requirements; analysis of nutrients (or active substances) of test fertilizers, or the company shall submit a recent test report from the statutory testing department. 4.2.3 Fertilization measures
Perform field operations according to the test plan and test fertilizer requirements. 4.2.4 Field management and observation records
4.2.4.1 Field management: Except for fertilization measures, other management measures shall be consistent and meet production requirements, and shall be completed by dedicated personnel in the same field.
4.2.4.2 Observation records: Field observation records include (see Appendix A for details): Experimental layout;
"Basic situation of the experimental site:
...room operation;
Biological traits;
Experimental results.
4.2.5 Harvest and yield calculation
Harvest and yield calculation should correctly reflect the experimental results: each plot is single-planted, single-harvested, single-yield calculated, or representative sample plots are taken for yield measurement; first harvest the plants in the protection row;
Crops that are harvested in batches, such as cotton, tomatoes, cucumbers, and watermelons, should be harvested and yield calculated in batches, and accumulated after harvesting; NY/T 497.--2002
Indoor test samples should be taken as required and labeled, recording the plot number, treatment name, sampling date, sampler, etc. 4.3 Experimental data analysis and fertilizer efficiency evaluation
4.3.1 Experimental data analysis
Statistical analysis of test results (see Appendix B for details): For paired design with two treatments, the t-test should be conducted according to the paired design; for completely randomized block design with more than two treatments, variance analysis should be used, and multiple comparisons should be performed using the PLSD method. 4.3.2 Fertilizer efficiency evaluation
The fertilizer efficiency evaluation (except microbial fertilizers) mainly compares the differences between treatment 1 and treatment 2; microbial fertilizers mainly compare the differences between microbial fertilizers and matrix controls. 4.3.2.1 Fertilizer products whose main function is to increase yields are effective if they meet the following indicators: the number of test points with an increase in yield of more than 5% in field trials is not less than two-thirds of the total number of test points; the difference is statistically significant in single-factor field tests. The number of test points with the same level shall not be less than two-thirds of the total number of test points. 4.3.2.2 Fertilizer products with the main function of improving quality or improving the environment can refer to the indicators of 4.3.2.1 of this standard selectively according to the specific situation. 4.4 Test report writing
4.4.1 Test source and purpose.
4.4.2 Test time and place.
4.4.3 Materials and methods:
…Test soil;
…Test soil Test fertilizers;
Test crops;
Test plan and methods.
4.4.4 Test results and analysis:
Effects of different treatments on crop biological traits; Effects of different treatments on crop yield and output value;… Input-output ratio of different treatments;
Statistical analysis results of test data.
4.4.5 Test conclusions.
4.4.6 Test execution unit and host.
NY/T 497.-2002
Test layout
Test location
Test time
Test plan design
Test treatment:
Number of repetitions:
Test method design
Plot area: length (m) × width (m) =
Plot arrangement: (using diagram)
Basic information of the test site
Topography of the test site:
Soil type:
Fertility level:
Name of previous crop:| |tt||Previous crop yield:
Soil analysis results:
Analysis items
Organic matter
Available phosphorus
Quick-acting potassium
Appendix A
(Normative Appendix)
Observation record form of field test for identification of fertilizer effectObservation record form of field test for identification of fertilizer effect Township
Soil texture:
Representative area:
Amount of fertilizer applied for previous crop: Organic fertilizer
Soil analysis results of test site
Nitrogen (N) Phosphorus (P,O,)
Potassium (K,0) Others
Analysis results
I-room operation
Tested crops
Sowing period and sowing products
Fertilization time and quantity
Irrigation time and quantity
Other farming activities and disasters
Biological traits of cattle
Test results
Test treatments
Treatment 1
Treatment 2
Treatment 3
Treatment 4||tt ||Experimental treatment
Treatment 1
Treatment 2
Treatment 3
Treatment 4
Repeat 1
Plot area
Repeat 2
Plot yield results
Repeat 1
Public yield results
kg/hm\
Repeat 3
Repeat 2
Repeat 4
Plot yield
Repeat 3
Average value
NY/T 497--2002bzxZ.net
Repeat 4
Yield increase rate
Average value
NY /T 497.--2002
Paired design (see Table B.1)
Treatment 1
Single standard deviation Sd
Mean standard deviation S:=
Appendix B
(Informative Appendix)
Example of analysis of test results
Statistics of paired design test results
Treatment 2
5. 31/2= 2. 66
[X,-X,1
40.75/2.6615.3
Unit is in grams per metric item
(d;d)2
Degree of freedom n—1—4—1-3, from the table, we get to.05=3.18, to.01-5.84. Because t=15.3≥to.ol—5.84, the difference between treatment 1 and treatment 2 is extremely significant, indicating that the fertilizer has an extremely significant effect. B.2
Randomized block design (see Table B.2)
Treatment!
Treatment 2
Treatment 3
Treatment 4
Statistics of randomized block design test results
Total sum of squares SS=（332+342++20°）Total degrees of freedom dff=4×4-1=15
Sum of squares between treatments SSA=（1282→1152+1102+79）÷4Degrees of freedom between treatments dfA4—1—3
Sum of squares between blocks SS#=（1102+113\+105+104）÷45 40
Unit is kilogram per metric item
Between-block variance d—4—1=3
Error sum of squares SS=382—323.5-13.5=45Within-treatment (error) variance df.—15—3—39The above results are tabulated for variance analysis (see Table B.3): Table B.3 Variance analysis of randomized block design
Between-blocks
Between treatments
Total variation
Sum of squares
Degrees of freedom
NY/T 497—2002
When F value ≥ F, it means that the difference between treatments is extremely significant, and "" is marked above the F value; when F\.>F value ≥ F, it means that the difference is significant, and "" is marked above the F value; when F value3 Analysis of variance for randomized block design
Between blocks
Between treatments
Total variation
Sum of squares
Degrees of freedom
NY/T 497—2002
When F value ≥ F, it means that the difference between treatments is extremely significant, and “” is marked above the F value; when F\.>F value ≥ F, it means that the difference is significant, and “” is marked above the F value; when F value3 Analysis of variance for randomized block design
Between blocks
Between treatments
Total variation
Sum of squares
Degrees of freedom
NY/T 497—2002
When F value ≥ F, it means that the difference between treatments is extremely significant, and “” is marked above the F value; when F\.>F value ≥ F, it means that the difference is significant, and “” is marked above the F value; when F value
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