This standard applies to calcium lactate synthesized from lactic acid and calcium hydroxide (or calcium carbonate) for use as a food additive. GB 6226-1986 Food additive calcium lactate GB6226-1986 Standard download decompression password: www.bzxz.net

National Standard of the People's Republic of China
Food additiveCalcium lactate
FoodadditiveCalcium lactate
GB/T6226-86
This standard applies to calcium lactate synthesized from lactic acid and calcium hydroxide (or calcium carbonate), used as a food additive. Molecular formula: C6H10Ca06·xH20
Molecular weight: 218.22 (anhydrous) (according to the international atomic weight in 1983) Technical requirementsbzxZ.net
1.1 Appearance: White to milky crystalline powder or granules, odorless. 1.2 Food additive calcium lactate should meet the requirements of the following table. Item
Calcium lactate content, %
Water solubility test
Loss on drying, %
Free acid (calculated as lactic acid), %
Free alkali
Volatile fatty acids
Arsenic (calculated as As), %
Heavy metals (calculated as Pb), %
Magnesium and alkali metals, %
2 Test method
By test
The reagents and water used in this standard are all analytically pure reagents, distilled water or ion exchange water. 2.1 Identification
2.1.1 Reagents and solutions
2.1.1.1 Potassium permanganate (GB643-77): 0.1N, prepared according to GB601-77. 2.1.1.2 Ammonium oxalate (HG3-976-81): 4% solution. 2.1.1.3 Sulfuric acid (GB625-77).
2.1.2 Identification method
In one sample solution, add sulfuric acid to make it acidic, add an appropriate amount of 0.1N potassium permanganate, and heat it, and the special odor of acetaldehyde will be generated. In another sample solution, add 4% ammonium oxalate solution, and a white precipitate will be generated, which is soluble in hydrochloric acid but insoluble in acetic acid.
2.2 Determination of calcium lactate content
2.2.1 Reagents and solutions
2.2.1.1 Disodium ethylenediaminetetraacetate (GB1401-78): 0.05M standard solution, prepared and calibrated according to GB601-77. 2.2.1.2 Sodium hydroxide (GB629-81): 10% solution. 2.2.1.3 Calcium mixed indicator: 0.1g calcium reagent carboxylic acid sodium salt and 20g sodium chloride dried at 110℃ (GB1266-77) are mixed and ground.
2.2.2 Determination method
Weigh 0.3g of the sample after loss on drying, accurate to 0.0002g. First, drop 25ml0.05M disodium ethylenediaminetetraacetic acid standard solution, add 5ml10% sodium hydroxide solution, 0.1g calcium mixed indicator, and continue to titrate with 0.05M disodium ethylenediaminetetraacetic acid standard solution until blue.
2.2.3 Expression and calculation of results
The percentage content of calcium lactate (X) is calculated according to formula (1). VXCX0.2182
Wherein: V-
Amount of disodium ethylenediaminetetraacetic acid standard solution, ml; Molar concentration of disodium ethylenediaminetetraacetic acid standard solution: -Weight of sample, g;
Number of grams of calcium lactate per milligram molecule.
2.3 Water solubility test
Weigh 1g of sample, accurate to 0.01g. Place in a colorimetric tube, add 20ml of water, heat in a water bath to dissolve, the solution should be colorless and almost clear.
2.4 Loss on drying
2.4.1 Determination method
Weigh 1g of sample, accurate to 0.0002g. Dry at 120℃ for 4h, calculate the percentage of loss on drying from the weight reduction, and it should meet the following requirements:
5 Water salt 22~27%;
3 Water salt 15~20%;
1 Water salt 5~8%;
Dry type is not more than 3%.
2.4.2 Expression and calculation of results
The percentage of loss on drying X1 is calculated according to formula (2). mi
Where: m1-
The weight reduction of the sample after drying, g;
-The weight of the sample, g.
2.5 Determination of free acid and free base
2.5.1 Reagents and solutions
2.5.1.1 Phenolic indicator solution: 1% ethanol solution, prepared according to GB603-77. (2)
2.5.1.2 Sodium hydroxide (GB629-81): 0.1N standard solution, prepared and calibrated according to GB601-77. 2.5.2 Determination method
Weigh 1g sample and weigh it to 0.01g. Dissolve in 20ml water. Add 2 drops of 1% phenolphthalein indicator solution, no red should be produced: add 0.5ml 0.1N sodium hydroxide standard solution, it should be red. 2.6 Determination of volatile fatty acids
2.6.1 Reagents
2.6.1.1 Sulfuric acid (GB625-77).
2.6.2 Determination method
Weigh 0.5g sample and weigh it to 0.01g, place it in dry evaporating blood, add 1ml sulfuric acid, heat it in a water bath, no fatty acid smell should escape.
2.7 Determination of arsenic
2.7.1 Reagents and solutions
2.7.1.1 Hydrochloric acid (GB622-77).
2.7.1.2 Potassium iodide (GB1272-77).
2.7.1.3 Arsenic-free metallic zinc (GB2304-80). 2.7.1.4
Stannoic chloride (GB638-78): 40% solution, prepared according to GB603-77. 2.7.1.5 Lead acetate cotton: prepared according to GB603-77. 2.7.1.6 Mercuric bromide test paper: prepared according to GB603-77. 2.7.1.7 Arsenic standard solution (1 ml solution contains 0.01 mg arsenic): prepared according to GB602-77 and diluted 10 times. 2.7.2 Instruments
Monument: According to the device in Figure 1 of GB610-77 "Determination of Arsenic". 2.7.3 Determination method
Weigh 1g sample, accurate to 0.01g. Place in a wide-mouth bottle, add 70ml water to dissolve, add 6ml hydrochloric acid, shake well, add 1g potassium iodide and 0.2ml 40% stannous chloride solution, shake well, and leave for 10min. Add 2.5g arsenic-free metal zinc, immediately install the glass tube with lead acetate cotton and mercuric bromide test paper, and place it in the dark at room temperature for 1 to 1.5h. The brown-yellow color of mercuric bromide test paper shall not be darker than the standard.
The standard is to take 0.3ml arsenic standard solution and treat it in the same way as the sample. 2.8 Determination of heavy metals
2.8.1 Reagents and solutions
2.8.1.1 Glacial acetic acid (GB676-78).
2.8.1.2 Saturated hydrogen sulfide water: Prepare according to GB603-77. 2.8.1.3 Lead standard solution (1 ml solution contains 0.1 mg lead): Prepare according to GB602-77. 2.8.2 Determination method
Weigh 1g sample, accurate to 0.01g. Dissolve in 1ml glacial acetic acid, dilute to 40ml, take 30ml, dilute to 40ml, add 10ml of freshly prepared saturated hydrogen sulfide water, shake well, and let stand for 10min. The dark color should not be darker than the standard. The standard is to take the remaining 10ml sample solution and 0.1ml lead standard solution and dilute to 40ml, and treat it in the same way as the same volume of sample solution.
2.9 Determination of magnesium and alkali metals
2.9.1 Reagents and solutions
2.9.1.1 Sulfuric acid (GB625-77).
2.9.1.2 Ammonia water (GB631-77).
2.9.1.3 Oxalic acid (HG3-988-76) 5% solution. 2.9.1.4 Methyl red indicator: 0.1% ethanol solution, prepared according to GB603-77. 2.9.2 Determination method
Weigh 1g sample, accurate to 0.01g. Heat and dissolve in 40ml water, add 40ml 5% oxalic acid solution, add 2 drops of 0.1% methyl red indicator, neutralize with ammonia water until the solution turns yellow, dilute to 100ml, shake well, leave overnight, filter, take 50ml, add 1ml sulfuric acid, evaporate on a water bath until almost dry, heat until sulfuric acid vapor escapes. Leach the residue with 25ml hot water, filter the filtrate and place it in a constant weight crucible, evaporate to dryness. Burn at 800℃ to constant weight. The weight of the residue shall not exceed 5mg. 3 Acceptance Rules
3.1 This product shall be inspected and accepted by the quality supervision department of the manufacturer in accordance with the product standards. The manufacturer shall ensure that all products leaving the factory meet the requirements.
3.2 The user department has the right to sample and accept batches of products. The sampling volume shall be 5% of the packaging volume of each batch. If there is one unqualified item or there is a dispute between the two parties, the number of samples shall be doubled and re-inspected. Even if there is one unqualified item, the entire batch of products shall be considered unqualified. If the supply and demand parties have objections to the inspection results, they can be handled in accordance with the relevant provisions of the "Food Hygiene Law of the People's Republic of China (Trial)". 3.3 The weight of each batch of this product shall not exceed 500kg. Packaging, marking, storage and transportation
4.1 Packaging: Packed in woven bags lined with polyethylene plastic bags, with a net weight of 15 to 20kg per bag. Seal to prevent moisture absorption. 4.2 The name of the manufacturer, product name, batch number, net weight and the words "food additive" should be stated on the outer packaging. Transportation and storage: During storage and transportation, care should be taken to keep the packaging intact, without damage or leakage. It is strictly prohibited to store or mix it with toxic substances. 4.3
Additional instructions:
This standard was proposed by the Ministry of Chemical Industry of the People's Republic of China and is under the jurisdiction of the Beijing Chemical Research Institute of the Ministry of Chemical Industry and the Food Hygiene Supervision and Inspection Institute of the Ministry of Health.
This standard was drafted by Xi'an Chemical Reagent Factory and Xi'an Health and Epidemic Prevention Station. The main drafters of this standard are Guo Zhujun, Zhu Zemin and Jiang Li. Issued by the State Bureau of Standards on March 26, 1986
Implementation on December 1, 1986
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