Standard number: GB/T 38505-2020
Standard name: General detection methods of genetically modified products
English name: General detection methods of genetically modified products ||
tt||Standard format: PDF
Release time: 2020-03-06
Implementation time: 2020-03-06
Standard size: 513K
Standard introduction: 1 Scope
This standard specifies the qualitative detection method of genetically modified products.
This standard is applicable to the real-time fluorescence PCR universal detection of genetically modified components in rice, corn, soybean, rapeseed, potato, beet, alfalfa, etc. and their processed products.
The minimum detection limit of this standard method is 0.1% (mass fraction).
This standard specifies the qualitative detection method of genetically modified products. This standard is applicable to the universal detection of genetically modified ingredients in rice, corn, soybean, rapeseed, potato, sugar beet, alfalfa and their processed products by real-time fluorescence PCR. The minimum detection limit of this standard method is 0.1% (mass fraction).

ICS07.080
iiiKAacJouaKAa
National Standard of the People's Republic of China
GB/T38505—2020
General detection methods of genetically modified products
General detection methods of genetically modified products2020-03-06Issued
State Administration for Market Regulation
National Standardization Administration
Implementation on 2020-03-06
This standard was drafted in accordance with the rules given in GB/T1.1-2009. This standard was proposed and managed by the National Technical Committee for Standardization of Biochemical Testing (SAC/TC387). iiiKAa~cJouaKAa
GB/T38505—2020
The drafting units of this standard are: China Inspection and Quarantine Scientific Research Institute, Shenzhen Yirui Biotechnology Co., Ltd., Inspection and Quarantine Technical Center of Liaoning Exit-Entry Inspection and Quarantine Bureau, Gansu Zhongshang Food Quality Inspection and Testing Co., Ltd., Inspection and Quarantine Technical Center of Guangxi Exit-Entry Inspection and Quarantine Bureau, Jilin Academy of Agricultural Sciences, Shanghai Institute of Metrology and Testing Technology Gansu Commercial Science and Technology Research Institute Co., Ltd. The main drafters of this standard are: Fu Wei, Wang Chenguang, Zhu Pengyu, Du Zhixin, Wei Shuang, Zhu Hai, Hong Xia, Zheng Qiuyue, Fu Shi, He Haining, Li Feiwu Jinhong, An Xiaoping, Zhu Shuifang, Ma Tao, Liu Gang, Yang Guangrui, Cao Jijuan, Yuan Shunjie, Huang Wensheng 1
1Scope
General detection method of genetically modified products
This standard specifies the qualitative detection method of genetically modified products. iiiKAa~cJouaKAa
GB/T38505—2020
This standard applies to the real-time fluorescence PCR universal detection of transgenic solid components in water frost, corn, soybean, rapeseed, potato, beet, radish, etc. and their processed products.
The minimum detection limit of this standard method is 0.1% (mass fraction) 2 Normative reference documents
The following documents are indispensable for the application of this document. For all dated reference documents, only the dated version applies to this document. For all undated reference documents, the latest version (including all amendments) applies to this document. GB/T6682 Specifications and test methods for analytical laboratories GB/T19495.1 Detection of genetically modified products
General requirements and definitions
GB/T 19495.3
GB/T19495.7
3 Terms and definitions
Detection of genetically modified products
Nucleic acid uptake and purification methods
Detection of genetically modified products
Sampling and sample preparation methods
The following terms and definitions apply to this document. 3.1
18SrRNAgene
18SrRNAgene
Transcribed from 18SrRNADNA, one of the components of cellular ribosomes, as an endogenous gene in plants. 3.2
CaMV 35S promoter 35S promoter from Cauliflower mosaic virus (Caulifloermnsaicwirus + CaMV) 3.3
CaMv35S terminator 35S terminator fromCamliflowermosaicvirus 35S terminator fromCauliflouermosatcwirus (CaMV) 3.4
NOs terminator of nopalinesynthase gene 3.5
E9 terminator of ribulose-1.5-biphosphate carboxylase small subunit 3' terminator sequence from ribulose-1,5-biphosphate carboxylase small subunit gene.
par gene
phosphinothricin acetyltransderase gene comes from Strebtonceuiridochromogene (phosphinothricin1
GB/T38505—2020
acetyltransderase.PAT).
Note, pa gene is tolerant to the herbicide glufosinate.3.7
Pin I terminator of proteinase inhibitor Ⅱ terminator from potato proteinase inhibitor Ⅱ (PinID).3.8
RbeS4 promoter promoter of ribulose-1.5-biphosphate carboxylase small subunit 1ATiiKAa~cJouaKAa
Promoter of the gene encoding ribulose-1,5-biphosphate carboxylase small subunit 1A (RbcS4) from Arabidopsis thaliana. 3.9
DAS402785 border sequence 5-endevent-specificsequence of DAS40278DAS40278 transformant exogenous insertion fragment 5 ° end and maize genome connection sequence. Note: This sequence includes part of the vector sequence of the exogenous temporary fragment and part of the library sequence of the maize gene red. 3.10
DP3054233 border sequence 3-end event-specific sequence of DP305423DP305423 transformant exogenous insertion fragment 3 end and soybean genome connection sequence. Note: This sequence includes part of the vector sequence of the exogenous fragment and part of the sequence of the soybean genome. 3.11
Cv1275' border sequence 5'-end event-specific sequence of Cv127CV127 transformant exogenous insertion fragment 5 end and soybean genome junction sequence. Note: This sequence includes part of the vector sequence of the exogenous temporary insertion fragment and part of the soybean genome sequence. 3.12
cyclethreshold
The number of cycles experienced when the fluorescence signal in the reaction tube reaches the set reading value. 4 Reagents and materials
Unless otherwise specified, all reagents are analytical grade or biochemical reagents. The test water meets the specifications of first-class water in GB/T6682. 4.1 Real-time fluorescence PCR premixwwW.bzxz.Net
Use the real-time fluorescence PCR premix that has been verified to meet the requirements of real-time fluorescence PCR. 4.2500mmol/L disodium ethylenediaminetetraacetic acid solution (pH8.0) Weigh 18.6g disodium ethylenediaminetetraacetic acid. Add it to 70mL water, and adjust the pH value to 8.0 with NaOH solution, add water to 100mL, and sterilize it at 103.4kPa (121C) for 20min. 4.31mol/L tris(hydroxymethyl)aminomethane-salt solution (pH8.9) Weigh 121.1g tris(hydroxymethyl)aminomethane and dissolve it in 800mL water. Adjust pH to 8.0 with hydrochloric acid. Add water to make up to 1000mL. Incubate at 103.4kPa (121C) for 20min. 4.4TE buffer (pH8.0)
Separately weigh 10mL 1mol/L tris(hydroxymethyl)aminomethane-salt solution (4.3) and 2ml.500mmol/l disodium ethylenediaminetetraacetic acid solution (4.2). Add water to make up to 1000mL. Incubate at 103.Sterilize at 4 kPa (121C) for 20 min2
Primers and probes
TiiKAa~cJouaKAa
GB/T38505—2020
Sequences of primers and probes for endogenous and exogenous gene or element detection are shown in Table 1. Primers and probes were diluted with TE buffer or double distilled water, and the dilution concentrations are shown in Table 1.
The 5 end of the probe is labeled with a fluorescent reporter group (such as FAM, HEX, etc.), and the 3 end is labeled with a fluorescent quencher group (such as TAMRA, BHQI, etc.)
Primer and probe information table
18SFRNA
Endogenous gene
CaMV35S
Promoter
CaMV35s
Terminator
NOS terminal positive
pat base
Piar terminator
E9 terminates at
RacS4 promoter
DAS402785| |tt||Border sequence
Primer name
Upstream primer
Downstream primer
Upstream primer
Downstream primer
Upstream primer
Downstream primer
Upstream primer
Downstream primer
Upstream primer
Downstream primer
Upstream primer
Downstream primer
Upstream primer
Downstream primer
Sequence (6'-3\ ) | |tt | CATGAGCG AAACCCTATAA | | tt | GC | | tt | ACAATTTC | | tt | |size/bp
GB/T385 05—2020
DP3054233
Boundary sequence
CV1275\
Near-boundary sequence
Primer name
Upstream primer
Downstream primer
Upstream primer
Downstream primer
Table 1 (continued)
Sequence (5'-3\)
CGTGTTCTCTTTTTGGCTAGC
GTGACCAATGAATACATAACACAAACTATGACACAAATGATTTTCATA CAAAAGTCGAGAAACAGAAGTTTCCGTTGAGCTTTAAGACCATTCGTAGCTCGGATCGTGTAC
TTTGGGGAAGCTGTCCCATGCCC
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Final dilution concentration
(nmol/L)
Target fragment
Size/bp
For samples that are not sure whether they are transgenic products, all endogenous and exogenous genes should be selected for detection. For transgenic samples without any genes, genes or elements should be selected for detection according to Table 2. Determine the species and select the gene or component
Soybean and its processed products
Rice and its processed products
Oil and its processed products
Watermelon and its processed products
Potato and its processed products
Sweet and its processed products
Instruments and equipment
Analytical balance: sensitivity 0.1mg.
Biological safety cabinet.
Real-time fluorescence PCR instrument.
Water purifier.
Vortex vibrator.
Micropipette.
Operation steps
Selected genes/elements
Endogenous gene, CaMV35S promoter, NOS terminator, pa gene, PinI terminator, E9 terminator, RbeS4 promoter.DP3054233 border sequence.CV1275 border sequence endogenous gene.CaMV35S promoter.CaMV35S terminator, NOS terminator, paI gene, Pi# terminator DAS402785\ border sequence endogenous gene Solid, CaMV35S promoter. CaMV35S terminator NOS terminator, E9 terminator. PinaⅡ terminator
Endogenous gene solid. CaMV35S promoter, CaMV35S terminator, NOS terminator Endogenous gene, NOS terminator, RkS4 promoter Endogenous gene, NOS terminator, E9 terminator Endogenous gene, E9 terminator
Follow the provisions of GB/T19495.1 and GB/T19495.7. 6.2 Sample preparation
Follow the provisions of GB/T19495.1 and GB/T19495.7 6.3 Sample pretreatment
Follow the provisions of GB/T19495.1 and GB/T19495.3. DNA template preparation
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GB/T38505—2020
Follow the provisions of GB/T19495.1 and GB/T19495.3. Alternatively, a plant-based solid tissue DNA extraction kit with the same effect can be used for DNA template preparation.
SDNA concentration determination
Use ultraviolet spectrophotometry to determine the DNA concentration. Dilute the DNA solution appropriately, measure its absorbance at 260nm, and calculate the DNA concentration based on the measured OD value (1OD at 260nm = 50pg/mL double-stranded DNA). The OD value should be in the range of 0.2--0.8. Measure its absorbance at 280nm, and calculate the ODzmam/ODmm ratio of the DNA solution based on the measured OD value. The ratio should be between 1.8 and 2.0
6.6 Real-time fluorescence PCR detection
6.6.1 Setting of negative control, positive control and blank control. The non-transgenic sample is used as the negative control. The corresponding transgenic plant sample strain or the genomic DNA of the transgenic plant sample containing the corresponding exogenous gene, or the plasmid standard molecule DNA containing the above fragment is used as the positive control, and water or TE buffer is used as the blank control. 6.6.2
Real-time fluorescence PCR reaction system
The PCR reaction system is shown in Table 3 or prepared according to the recommended system of the kit that has been verified to meet the requirements. Prepare 2 parallel tubes for each DNA sample. When adding samples, the sample DNA should be completely added to the reaction solution without sticking to the tube wall. The tube cap should be tightly closed as soon as possible after adding samples. Table 3
Real-time fluorescence PCR reaction system
Reagent name
Real-time fluorescence PCR premix
Upstream primer (10pmot/L)
Downstream primer (0μmel/L
Probe (10pmol/L)
DNA template (50ng/μL)
Add water to
0.4 μmol/1
0.4 μmol/L
0.2 pmol/L
4.0 ng/μl
The amount of each reagent in the reaction system can be adjusted appropriately according to the total volume of the reaction system. Instrument settings
Sample volume/nt
Set the fluorescence signal collection conditions of the PCR reaction tube, which should be consistent with the reporter group labeled by the probe. For specific setting methods, please refer to the instrument manual.
GB/T385052020
PCR reaction parameters
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Real-time fluorescence PCR amplification parameters: 50C/2min: 95C/10min: 95C/15s.60C/60s, greater than or equal to 40 cycles.
Note: 95C /10min is specially suitable for hot start Ta enzyme with chemical conformational change. The above parameters can be adjusted according to different models of real-time fluorescence PCR instrument and the selected PCR amplification reagent system.
SPCR reaction operation
Place the PCR reaction tubes on the real-time fluorescence PCR instrument in sequence. Before loading the instrument, check whether the reaction tubes are tightly covered to prevent light leakage and contamination of the instrument. Start running the instrument for real-time fluorescence PCR reaction. 7. Result analysis
7.1 Threshold setting
After the real-time fluorescence PCR reaction is over, set the fluorescence signal threshold. The threshold setting principle is adjusted according to the instrument noise situation, and the threshold line just exceeds the highest point of the normal negative sample amplification curve. 7.2 Quality control
Blank control: The internal reference gene detection does not show a typical amplification curve, all exogenous gene detections do not show a typical amplification curve, or the value is greater than or equal to 40.
Expression control: The internal reference gene detection shows a typical amplification curve, and the value is less than or equal to, all exogenous gene detections do not show a typical amplification curve, or the threshold value is greater than or equal to 40. Positive control: The detection of the internal reference gene shows a typical amplification curve, and the Ct value is less than or equal to 30; the detection of all exogenous genes shows a typical amplification curve, and the Ct value is less than or equal to 34. 8 Result judgment and expression
8.1 Result judgment
If all the test samples are reacted in parallel, the detection of the exogenous gene does not show a typical amplification curve or the C value is greater than or equal to 0: The detection of the endogenous gene shows a typical amplification curve, and the Ct value is less than or equal to 30, it can be judged that the sample does not contain the exogenous gene tested. If all the test samples are reacted in parallel, the detection of the exogenous gene shows a typical amplification curve, and the Ct value is less than or equal to 36. The detection of the endogenous gene shows a typical amplification curve, and the Ct value is less than or equal to 30, it can be judged that the sample contains the corresponding exogenous gene. If all the test samples are reacted in parallel, the detection of the exogenous gene shows a typical amplification curve, but the Ct value is between 36 and 40, and the Ct value of the endogenous gene detection shows a typical amplification curve and is less than or equal to 30, the sample should be reprocessed and tested on the machine after eliminating contamination. After re-amplification, if the endogenous gene detection shows a typical amplification curve, and the Ct value is less than or equal to 30, and the exogenous gene detection shows a typical amplification curve, and the t value is still less than 40, it can be determined that the sample contains the exogenous gene to be detected. After re-amplification, if the endogenous gene detection shows a typical amplification curve, and the Ct value is less than or equal to 30, and the exogenous gene detection does not show a typical amplification curve, or the Ct value is greater than or equal to 40, it can be determined that the sample does not contain the exogenous gene to be detected.
8.2 Result expression
As follows:
No exogenous gene was detected in the sample.
Exogenous gene was detected in the sample.
Storage period of sample for inspection
The storage period of sample for inspection shall be implemented in accordance with the provisions of GB/T19495.1. Transformant information
For the transformant information covered by this standard, please refer to Appendix A. iiKAa~cJouaKAa
GB/T38505—2020
GB/T38505—2020
For information on the transformation covered by the standard, please see Table A.1
Number and variety survey
MON810
MON89034
MON88017
MON87460
MON87427
MON863
MIR604
MIR162
TC1507
VCO-01981-5
DAS40 278
GTS40-3-2
MON89788
MON87701
MON87705
MON87708
MON87769
MON87751
DP305423
DP356043
Promoter
Appendix A
(Informative Appendix)
Standard Coverage Transformant Information
Table A.1 Standard Coverage Transformant Information
Terminator
Terminator
Terminator
Terminator
Promoter
pat gene
bee stopper
TiiKAacJouaKA
DAS40278
DP305423CV122
A5547-127
A2704-12
SYHTOH2
DAS44406-6
DAS68416-4
DAS81419
RT73(GT73
MON88302
Topas19/2
OXY-235
LL RICE62
TT51-1
AM04-1026
EH92-527-1
PH05-026-0048
AV-42-6-G7
Promoter
Terminator
RibeS4
Promoter
par基国
Terminator
TiiKAacJouaKA
GB/T38505—2020
D/AS10278 DP305423CV1260C/60s, greater than or equal to 40 cycles.
Note: 95C/10min is specifically suitable for hot start Ta enzymes with chemical conformational changes. The above parameters can be adjusted according to different models of real-time fluorescence PCR instruments and the selected PCR amplification reagent system.
SPCR reaction operation
Place the PCR reaction tubes in turn on the real-time fluorescence PCR instrument. Before loading the instrument, check whether the reaction tubes are tightly covered to prevent light leakage and contamination of the instrument. Start running the instrument for real-time fluorescence PCR reaction. 7. Result analysis
7.1 Threshold setting
After the real-time fluorescence PCR reaction is completed, set the fluorescence signal threshold. The threshold setting principle is adjusted according to the instrument noise situation, and the threshold line just exceeds the highest point of the normal negative sample amplification curve. 7.2 Quality control
Blank control: The internal reference gene detection does not show a typical amplification curve, all exogenous gene detections do not show a typical amplification curve, or the value is greater than or equal to 40.
Positive control: The detection of the internal reference gene shows a typical amplification curve, and the Ct value is less than or equal to 30, and the detection of all exogenous genes shows no typical amplification curve, or the Ct value is greater than or equal to 40. Positive control: The detection of the internal reference gene shows a typical amplification curve, and the Ct value is less than or equal to 30, and the detection of all exogenous genes shows a typical amplification curve, and the Ct value is less than or equal to 34. 8 Result judgment and expression
8.1 Result judgment
All test samples are reacted in parallel. The detection of exogenous genes does not show a typical amplification curve or the Ct value is greater than or equal to 0: The detection of endogenous genes shows a typical amplification curve, and the Ct value is less than or equal to 30, then it can be judged that the sample does not contain the exogenous gene being tested. All test samples are reacted in parallel. The detection of exogenous genes shows a typical amplification curve, and the Ct value is less than or equal to 36. The detection of endogenous genes shows a typical amplification curve, and the Ct value is less than or equal to 30, then it can be judged that the sample does not contain the exogenous gene being tested. All test samples are tested in parallel. The detection of exogenous genes shows a typical amplification curve, but the Ct value is between 36 and 40. The detection of endogenous genes shows a typical amplification curve and the Ct value is less than or equal to 30. The sample should be reprocessed and tested on the machine after eliminating contamination. The detection of endogenous genes after re-amplification shows a typical amplification curve, and the Ct value is less than or equal to 36. The detection of exogenous genes shows a typical amplification curve, and the Ct value is still less than 40. It can be determined that the sample contains the exogenous gene to be tested. The detection of endogenous genes after re-amplification shows a typical amplification curve, and the Ct value is less than or equal to 30. The detection of exogenous genes does not show a typical amplification curve, or the Ct value is greater than or equal to 40. It can be determined that the sample does not contain the exogenous gene to be tested.
8.2 Result expression
is as follows:
No exogenous gene was detected in the sample.
Exogenous gene was detected in the sample.
Storage period of samples for inspection
The storage period of samples for inspection shall be in accordance with the provisions of GB/T19495.1. Transformant information
For information on transformants covered by this standard, please refer to Appendix A. iiKAa~cJouaKAa
GB/T38505—2020
GB/T38505—2020
For information on the transformation covered by the standard, please see Table A.1
Number and variety survey
MON810
MON89034
MON88017
MON87460
MON87427
MON863
MIR604
MIR162
TC1507
VCO-01981-5
DAS40 278
GTS40-3-2
MON89788
MON87701
MON87705
MON87708
MON87769
MON87751
DP305423
DP356043
Promoter
Appendix A
(Informative Appendix)
Standard Coverage Transformant Information
Table A.1 Standard Coverage Transformant Information
Terminator
Terminator
Terminator
Terminator
Promoter
pat gene
bee stopper
TiiKAacJouaKA
DAS40278
DP305423CV122
A5547-127
A2704-12
SYHTOH2
DAS44406-6
DAS68416-4
DAS81419
RT73(GT73
MON88302
Topas19/2
OXY-235
LL RICE62
TT51-1
AM04-1026
EH92-527-1
PH05-026-0048
AV-42-6-G7
Promoter
Terminator
RibeS4
Promoter
parbase
Terminator
TiiKAacJouaKA
GB/T38505—2020
D/AS10278 DP305423CV1260C/60s, greater than or equal to 40 cycles.
Note: 95C/10min is specifically suitable for hot start Ta enzymes with chemical conformational changes. The above parameters can be adjusted according to different models of real-time fluorescence PCR instruments and the selected PCR amplification reagent system.
SPCR reaction operation
Place the PCR reaction tubes in turn on the real-time fluorescence PCR instrument. Before loading the instrument, check whether the reaction tubes are tightly covered to prevent light leakage and contamination of the instrument. Start running the instrument for real-time fluorescence PCR reaction. 7. Result analysis
7.1 Threshold setting
After the real-time fluorescence PCR reaction is completed, set the fluorescence signal threshold. The threshold setting principle is adjusted according to the instrument noise situation, and the threshold line just exceeds the highest point of the normal negative sample amplification curve. 7.2 Quality control
Blank control: The internal reference gene detection does not show a typical amplification curve, all exogenous gene detections do not show a typical amplification curve, or the value is greater than or equal to 40.
Positive control: The detection of the internal reference gene shows a typical amplification curve, and the Ct value is less than or equal to 30, and the detection of all exogenous genes shows no typical amplification curve, or the Ct value is greater than or equal to 40. Positive control: The detection of the internal reference gene shows a typical amplification curve, and the Ct value is less than or equal to 30, and the detection of all exogenous genes shows a typical amplification curve, and the Ct value is less than or equal to 34. 8 Result judgment and expression
8.1 Result judgment
All test samples are reacted in parallel. The detection of exogenous genes does not show a typical amplification curve or the Ct value is greater than or equal to 0: The detection of endogenous genes shows a typical amplification curve, and the Ct value is less than or equal to 30, then it can be judged that the sample does not contain the exogenous gene being tested. All test samples are reacted in parallel. The detection of exogenous genes shows a typical amplification curve, and the Ct value is less than or equal to 36. The detection of endogenous genes shows a typical amplification curve, and the Ct value is less than or equal to 30, then it can be judged that the sample does not contain the exogenous gene being tested. All test samples are tested in parallel. The detection of exogenous genes shows a typical amplification curve, but the Ct value is between 36 and 40. The detection of endogenous genes shows a typical amplification curve and the Ct value is less than or equal to 30. The sample should be reprocessed and tested on the machine after eliminating contamination. After the endogenous gene detection is amplified again, a typical amplification curve is shown, and the Ct value is less than or equal to 36. The detection of exogenous genes shows a typical amplification curve, and the Ct value is still less than 40. It can be determined that the sample contains the exogenous gene to be detected. After the endogenous gene detection is amplified again, a typical amplification curve is shown, and the Ct value is less than or equal to 30. The detection of exogenous genes does not show a typical amplification curve, or the Ct value is greater than or equal to 40. It can be determined that the sample does not contain the exogenous gene to be detected.
8.2 Result expression
is as follows:
No exogenous gene is detected in the sample.
Exogenous gene is detected in the sample.
Storage period of samples for inspection
The storage period of samples for inspection shall comply with the provisions of GB/T19495.1. Transformant information
For information on transformants covered by this standard, please refer to Appendix A. iiKAa~cJouaKAa
GB/T38505—2020
GB/T38505—2020
For information on the transformation covered by the standard, please see Table A.1
Number and variety survey
MON810
MON89034
MON88017
MON87460
MON87427
MON863
MIR604
MIR162
TC1507
VCO-01981-5
DAS40 278
GTS40-3-2
MON89788
MON87701
MON87705
MON87708
MON87769
MON87751
DP305423
DP356043
Promoter
Appendix A
(Informative Appendix)
Standard Coverage Transformant Information
Table A.1 Standard Coverage Transformant Information
Terminator
Terminator
Terminator
Terminator
Promoter
pat gene
bee stopper
TiiKAacJouaKA
DAS40278
DP305423CV122
A5547-127
A2704-12
SYHTOH2
DAS44406-6
DAS68416-4
DAS81419
RT73(GT73
MON88302
Topas19/2
OXY-235
LL RICE62
TT51-1
AM04-1026
EH92-527-1
PH05-026-0048
AV-42-6-G7
Promoter
Terminator
RibeS4
Promoter
par基国
Terminator
TiiKAacJouaKA
GB/T38505—2020
D/AS10278 DP305423CV121
Number Survey
MON810
MON89034
MON88017
MON87460
MON87427
MON863
MIR604
MIR162
TC1507
VCO-01981-5
DAS40278
GTS40-3-2
MON89788
MON87701
MO N87705
MON87708
MON87769
MON87751
DP305423
DP356043
Promoter
Appendix A
(Informative Appendix)
Standard Coverage Transformant Information
Table A.1 Standard Coverage Transformant Information
Terminator
Terminator
Terminator
Promoter
pat gene
Terminator
TiiKAac JouaKA
DAS40278
DP305423CV122
A5547-127
A2704-12
SYHTOH2
DAS44406-6
DAS68416-4
DAS81419
RT7 3(GT73
MON88302
Topas19/2
OXY-235
LLRICE62
TT51-1
AM04-1026
EH92-527-1
PH05-026-0048
AV-42-6-G7
Promoter
Stop
Stop
Table A.1 (continued)
Terminator
RibeS4
Promoter
parbase
Terminator
TiiKAacJouaKA
GB/T38505—2020
D/AS10278 DP305423CV121
Number Survey
MON810
MON89034
MON88017
MON87460
MON87427
MON863
MIR604
MIR162
TC1507
VCO-01981-5
DAS40278
GTS40-3-2
MON89788
MON87701
MO N87705
MON87708
MON87769
MON87751
DP305423
DP356043
Promoter
Appendix A
(Informative Appendix)
Standard Coverage Transformant Information
Table A.1 Standard Coverage Transformant Information
Terminator
Terminator
Terminator
Promoter
pat gene
Terminator
TiiKAac JouaKA
DAS40278
DP305423CV122
A5547-127
A2704-12
SYHTOH2
DAS44406-6
DAS68416-4
DAS81419
RT7 3(GT73
MON88302
Topas19/2
OXY-235
LLRICE62
TT51-1
AM04-1026
EH92-527-1
PH05-026-0048
AV-42-6-G7
Promoter
Stop
Stop
Table A.1 (continued)
Terminator
RibeS4
Promoter
parbase
Terminator
TiiKAacJouaKA
GB/T38505—2020
D/AS10278 DP305423CV12
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