General directives for the conduct of tests of distinctness,uniformity and stability for new varieties of plants
Some standard content:
[CS 65.020.20 National Standard of the People's Republic of China GB/T19557.1—2004 Ceneral directives for the conduct of tests of distinctnessuniformity and stabilily for new varielies of plants2004-06-11 issued
General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China Standardization Administration Committee
2004-12-01 implementationbZxz.net
CB/T13557.1—2004
Model references
Technical economy and definitions
DLS test requirements
Test characteristics
Specialty identification method
Respect for property judgment method
3 Qualitative identification method for non-Southern villages
A specification for commercial pools)
Appendix specification is also recorded online!
Technical Questions and Conditions
Testing and Reporting Format of New Plant Varieties
This standard is except for Appendix A. Appendix B is the appendix of this standard. This standard is issued by the Agricultural Science and Technology Development Center of the Ministry of Agriculture. The main parties of this standard are: the Ministry of Agriculture, ... 19557.1—20Q4
This standard specifies the general principles for formulating the test guide for new plant varieties (hereinafter referred to as ILS), the general technical requirements for conducting the test, the determination method and the technical basis for the test results, and the format.
This standard is based on the relevant testing conditions for new plant products during use, and is used to determine or identify the heterogeneity, consistency and qualitative properties of new plant varieties through their characteristics. 2 Normative referenced documents
The clauses in the following documents become clauses of the standard through reference in this standard. The dated referenced documents, all subsequent amendments (excluding errata or revisions) and the standards are not applicable to wood standards, however, the latest editions of these documents may not be used by the parties concerned. The product edition year is not applicable to this standard. GB clothing crop cloth brown collection idea || tt || JGP/! Light heterogeneous test (FxiriningTislim:luessTCP—Test (ExamiimpTnifarmiyIGP Qualitative test (FxamiangStaslity) TGP/Drf2TG/Zhongtejun period total household editing fee understand music unified i party CYD (IumctTCP/U\Exaaiaing ianckars\Seetan Tr/n,i.Racamncrded Stniotien Mohuc~cyL)IGP/1u.3.1Jraft2TGP/lu in a political test recommended system: COYL (TncumertIG2: 1\Examiniux I.uifunmi.yswr:in TGP/10. d. I.Rmnr.mended S.utist.cal Methodls: COY J) 3 Terms and Definitions
The following terms and definitions apply to this standard. 3.1
Variety
A plant variety of a known plant species. Whether or not a new variety meets the following conditions:
A plant variety that has a characteristic phenotype and is distinguished from other plants by the expression of at least the above characteristics;
A plant variety that has been artificially bred or developed from a wild plant species and has new, distinctive, unique and unique characteristics and is appropriately named;
Application variety and idute vnriety
The products that have been applied for protection of the variety rights should be tested for distinctiveness, consistency and total innovation. GB/19557.1—2004
Known varieties are the existing known varieties, including new varieties that have been applied for protection, and the designated varieties, which are described in publications in the region. The varieties that can be bred,
The variety that is closest to the known varieties, such as the relevant certificates, etc., are the most similar to the Chinese varieties. 3.6
Standard variety exumplevnriel3
In the test of new varieties of citrus, the reference standard of character classification is used to judge the reliable positioning of new varieties. 3.7
Holding heterosexual distinctiveness
3.8
uniformities
The new plant varieties for which the application for variety right is submitted shall be clearly distinguished from the specification varieties that have been designated before the submission of the application for approval. 3.9
stability
The new plant varieties for which the application for variety right is submitted shall, after breeding, except for the changes found in the items, have their specific characteristics or various properties unchanged after the new plant varieties have been bred or at the end of a specific breeding cycle. 3.11
DUS test
The approval authority shall entrust the relevant test structure to be determined, adopt the relevant test technology and standards ([test measures]), and conduct the specification test [internal and external] or indoor analysis test on the products and the specificity, efficacy and qualitative properties. 3.12
Dust
TTIS test guide
is the first technical specification of the first level of the organization's work, and also the market approval of new products, the standard of the competition for the determination of the components,
Dts to test the characteristics of hararterlstlcsusedmDlisting used to conduct DLS test "characteristics", including the external shape of the body (quality characteristics, quantitative characteristics and single characteristics) the main analytical part (specific characteristics of the new characteristics), the text can be divided into basic characteristics, essential characteristics, grouping and supplementary characteristics, 3. t4
Quality traits qualitat/vecheracteristles plants in the present small continuous variation traits related to the field sex: male plants into sex description (1) the beginning of the taste of the plant (2 carving sleep to the ball of the total sex 3). Attached to the ball of the two Huayan (Ting, 3.15
number of disc traits qwantitatlvecharacteristies compensation to continue, carrying the equivalent of the full description of the traits, it shows the traits from: one extreme to the other extreme of the continuous change. For Ding a certain - the description of the trait will be described in several times [such as stem length, very short 1) from (3) single (5), long (7) very long 9). 2
from quality makeup psudur-yualitatichuraclurisicsCB/I *9557.1—2094
The expression of a trait is at least partially continuous and its range of variation is multiple: oval (1-shaped (>), angular (5), ovate (door, new expressions need to be determined within the trait description. 3. 1/
Special traits are traits other than quantitative traits, quantitative traits, and quantitative traits, including resistance traits, product traits, and combined traits. 3. 18
Resistance traits are the responses to external factors, such as living organisms [disease resistance traits) or chemical substances (weed resistance traits>). 3.19
Quality traitscharacteristicexpressed withchemical constituents, such as end content, lipid content3.20
Combined traitscombinedcharacteristis a combination of non-characteristics, single combination of traits, length-to-width ratio. 3.21
New traitstypesnewtypesnfcharacleristirsplantation morphologyspecial main traits, such as, divided into traits:3. 22
Based on the most basic and well-tested varieties, the ice shape was adopted. 3.23
to characterize the cat breed used in international standard breed description of the world's important test characters, including all non-sequential characters in the original index 3.24
grouping character groupingcharacteristic character grouping character 3.25
additionalchararteristiccharacteristic not yet tested by human beings, including some new characters. Addition: white character, molecular characteristics: 3. 26
heteromorphic strain (atypical dead strain)
nff-type
a plant in the same breed that is in normal growth condition, but the majority of its body parts have obvious differences in morphology
variation Variance
Type (non-real city miserable point urgent industry value of the rate, 4DUS test basic requirements
4.1131S test report
In the Liu specific range of the test: should be in accordance with the heart of your foreign proposed rules and requirements, Taiwan cabinet new variety DUS test guide provisions, the same as the DS test single technical basis 3
CB/T 19557,1—2004
.2 US test design
Design of the test kit before testing or other clearance tests: The design file shall include the following contents, test period, test layout, test quantity, observation method, etc.
2.3 DLS test characteristics
The product must be accurately described step by step, and then the requirements can be provided to review whether it meets the "S standard". The "S" variety is specified to describe its growth conditions, because these characteristics are more important than IS test growth conditions, which are strictly included in the technical volume (see Appendix A). 4.4 Material requirements for S test
4. 4.1 Quantity and quality of materials
The number and quality of materials submitted for testing shall be in accordance with the requirements. For materials that need to be tested, the manufacturer must ensure that all materials of the element are available at the time of testing. 4.4.? Representative implant materials
The whole test materials should be typical of the applied product and similar varieties, varieties with specific elements, and must be a combination of two varieties, with the longest shelf life.
4.4.3 Material health requirements
The materials submitted for testing shall be of the highest quality. The materials should be healthy, vigorous and free from diseases. Seeds should meet the requirements of G354.
4.4.4 Material treatment
When any treatment is required for materials that do not meet the requirements, it must be approved by the examination and approval authority, and the method and process should be described in detail. 4.5 Test record and report requirements
The original data such as the period, point, method, stage, observation results, calculation process, etc. of the test should be recorded in a unified form, and the calculated results should be converted into a code format for output. The original claim must be reviewed and approved by the market. 4.6 If any infringement occurs after the investment, the applicant shall conduct quantitative testing and the whole breeding materials and production materials submitted by the applicant when submitting the application shall be tested for specificity, efficacy and stability. 5DLI test traits
5.1 Selection of traits
The selected product shall report the basic traits of the product, that is, the results of the combination of specific drugs or compounds shall be added as follows: h) Under a certain environment, it has sufficient usability to determine the specific superiority of the product; h) There is enough difference in the product tax table to determine the specific superiority! Can be determined and identified
heart) to achieve an effective external requirement:
meet the requirements of stability, that is, after each reproductive cycle, the result is only one sex and egg phenomenon, the listed traits have two industry value improvement needs, as the world's economic benefits can reach the level of continuous consumption, and can be used as US test supplementary traits.
5.2 Expression state of traits
The state in the test form is divided into different states: each state is described by a code and its corresponding trace: the expression of the trait allows the class to collect the source of the money. In order to better determine the variation, the test product is used to clarify the expression of the traits. 5.3 Types of trait expression In the test, the test traits are divided into three categories: whole body traits, whole body traits, and single traits. 5.2 Observation of traits In the test, the test area, sample size, number of replicates and growth cycle should be clearly defined in the test so as to determine and compare the traits. 5.5 Special traits 5.5.1 Anti-proliferation traits G1/T 19557.1--2004 3.1 Requirements: Such traits should be accurately defined and appropriate test methods should be selected to ensure the consistency of the test.
5.5.2 Quality traits
The properties expressed by the chemical components of the base should meet the requirements of .1. Therefore, it is recommended to define the trait and select appropriate test methods to ensure the consistency of the test.
5.5.3 Combination traits
Combination traits with biological significance can be directly used as test materials, and other traits can be tested for consistency and stability. For these combination traits, the evaluation should use technical means such as image analysis. 5.6 New trait testing
When testing, new trait types including the classified traits can be used. 5.7 Functional classification of traits
The functional classification of traits is shown in Table 1.
Basic work of testing method
Matching traits
Classification technology
Foreign production length
6 Specific identification method
6.1 Differentiation of new varieties
6.1.1 Variety comparison
Functional classification table of traits
PV recognizes two S secondary selections: Each component can be selected from, 1. Naturally achieve.: The extremely determined [S trait is used quickly to note the makeup of the country's conditions.
The level of the agent should be able to apply the actual variety of the delay and property requirements: the source of the standard
2. The description of the product must be made in a complete pressure
3, the loss of personality characteristics will be explained by the hand of the other ministers
1. This type of property will be its necessary activity. And be prepared by the members of the Sichuan works to point out the selection,
2. Should be particularly cautious about the salt reaching rate of the elimination of Lan I as the radical change than Hong.
To be sent as a net recent fake product book according to the fee: in the special guide 1. It can be the quality gas state, the policy decision or the reading layer of the grass property test will exclude the public variety related to the property from the school to let the school
2. The test period of the cocoa variety can be delayed in the same memory,
. In the use of the month: for the future
DLS intense test guide to prepare the properties of compensation. However, the records of the differences in the area cannot be used to determine the activity of the species. 2. In the end, the external state in the technical document must be secreted. 2. In theory, the specific test of the variety will be compared with all other products. However, the actual operation method is to compare the single 5 GA/T 19557.1—2C04
The product should be compared with the varieties already mentioned and their characteristics should be identified. If the variety can be identified by comparing the variety descriptions in the previous article, then planting tests are necessary: otherwise, planting tests should be carried out to determine the characteristics of the product and its characteristics. For details, see the International Union for the Protection of New Plants document 1TGP/9).
This document (Appendix A) indicates the important characteristics of the variety most similar to the application variety. These varieties should be compared with the application variety according to the planting plan.
6.1.2. Differentiating varieties by trait differences
If a trait only causes a difference in appearance, but does not cause a difference in the overall expression of the trait in the variety, then this difference cannot be used as evidence to determine specificity. 6.1.3 Specificity discrimination
If a trait of a variety is significantly different in appearance, then the variety is considered to have significant differences. 6.1.3.1 Constancy of differences
To demonstrate that a trait observed in a planting trial is constant, the trait should be tested in at least two independent situations. This can be done by planting annual and perennial varieties in two different soils and observing them separately. Or for perennial varieties, observing them over two different growing seasons after one planting, subjecting them to small conditions, such as temperature and light. Two growing seasons should be observed.
A new variety of a related species should be observed in the same growing period as required to show constancy of trait (planned growth in two independent locations or environments). 6.1.3.2 The difference between the varieties depends on the actual reason. The difference between the two varieties depends on the expression of the tested trait, that is, the expression of the tested trait, the quantitative trait or the pseudo-quantitative trait. 6.3.2.1 Quality traits For quality traits, if the expression of the trait is different in the test index, the two varieties are considered to have different characteristics. If the expression of the trait is the same, the difference is not considered to be obvious. 6.1.3.2.2 Quantitative traits The test method based on the variety breeding method is to use the quantitative characteristics as the specific characteristics. Other methods are specified in 6 and 53 of this standard. E, 1.3.2.3 Pseudo-quality traits The test guide for different levels may not be sufficient to determine the difference. 6.2 Explanation of observations without statistical methods for evaluating specificity If the cultivars themselves are extremely variable, the specificity can be determined by statistical methods. If the expression of one or more of the growth characteristics of two varieties is not a criterion in the test guide, it can be assumed that the two varieties have significant differences. For quantitative differences, the differences in the expression of the two characteristics are usually obvious, but this does not define slight differences. Because the products are affected by factors such as location, year, and environmental differences in expression, the differences can be small or large. The DS test method for new varieties of plants and animals shall not be used to determine the specificity. 6.3 Explanation of observations without statistical methods for evaluating specificity If the cultivars themselves are extremely variable, the specificity can be determined by statistical methods. If the expression of one or more of the growth characteristics of two varieties is not a criterion in the test guide, it can be assumed that the two varieties have significant differences. For quantitative differences, the differences in the expression of the two characteristics are usually obvious, but this does not define slight differences in expression. Because the products are affected by factors such as location, year, and environmental differences in expression, the differences can be small or large. The DS test method for new varieties of plants and animals shall not be used to determine the specificity. For the interpretation of the test results, an appropriate method should be chosen. Only the combined test traits must meet the efficacy criteria. Only the combined test traits can be used. Special traits: 6.3.1 Test traits 6.3.1.1 Quality traits (GH/T19557.1-2004) are often used to evaluate the quality of production traits. The expression of the trait can be directly compared with the expression of the trait. Under normal circumstances, there is no need to use statistical methods to analyze the results and make a determination. E.2.1.2 Quantitative traits In addition to measurement and counting, quantitative traits can be evaluated by other methods. Since direct pairwise comparison is not necessary, it is recommended to compare two similar varieties directly. If there are obvious differences between the varieties in the pairwise comparison (for example, for the expression of a certain trait, variety A is always better than variety 10), and it can be reproduced in the modified test: the product has a certain number of differences, then it is clear that the product is different. E.3.1.3 False quality traits |Whether the evaluation of general quality traits makes the research method determine the specific situation, E.3.2 Quality traits
6.3.2. Self-pollinated and asexually propagated varieties, at the appropriate growth stage and specified level, if the difference in the quality of the two varieties is greater than the small difference in maturity (1 door), even if the expression of the state is busy, it is considered that there is only a significant difference in growth, 6.3.2.2 Cross-pollinated varieties
The specificity of heterogeneous tree varieties should be determined by the analysis method: it can refer to the "comprehensive analysis method of specificity and treatment of annual" (\)YD> of the International Plant Variety Protection Association, this method takes into account the variation of the year. 7 Consistency judgment method
7.1 Related traits
Any obvious growth condition 7.2. Determination of homogeneity of varieties
The requirement for consistency of varieties should be based on the test results. If all plants of a variety are very similar, especially for the unpollen varieties and the pollinated flowers, the obvious differences in the production (opening type) can be compared with all the plants of the middle variety through the variation range of the medium. In order to determine whether it is old, you can estimate the test number.
7.2. Self-pollinating and asexually propagated varieties
7.2.7.1 Determination of heteromorphic plants by visual inspection In the 1US test, the expression of all or part of the traits of a certain variety is consistent with the trait expression of the variety. When evaluating the heterotypic characteristics of a species, the determination of differences between heterotypic samples and marketed products and between common varieties and common varieties is generally the same as in Section 4.3.11. 7.2.1.2 Use measurement methods to determine heterotypic characteristics of self-pollinated varieties and wood varieties obtained by comparing the majority of common traits of a group of plants obtained at one time, etc. The purpose is to: estimate the heterotypic characteristics of self-pollinated, mouth-pollinated and normally-pollinated varieties: The method for processing the measurement data of a single plant can be used as a document related to the International Union for the Protection of New Varieties of Plants (TPP1017.2.1.3 Determine the number of heterotypic samples and the rate of increase in tree roots, and determine the maximum allowable value of the daily height of a small heterotypic plant. If it is possible to determine the size of all plants of a variety according to the percentage of acceptable morphological characteristics, the population size should be determined in the pool area and the maximum allowable value of the morphological characteristics should be clearly stated in the test documents of the new variety. The specific method can be found in the International Plant Variety Protection Association (TGPO) 1.2.1.3.1 Special provisions for sexually propagated and strictly self-pollinating varieties and varieties without the potential for self-fertilization. The maximum allowable value of morphological characteristics can be found in the three. T
GB/T19557.1—2G04
Table 2 Relationship between sample size and allowable value of heteromorphic varieties
36--82
$3--137
:58 --198
:09XR:
7.2.1.3.2 Interaction of parental inbred lines of cross-pollinated varieties and hybrids
In the test households, cross-pollinated varieties are often used in combination with self-pollinated varieties. Compared with cross-pollinated varieties and non-standard varieties, the allowable values of parental inbred lines of cross-pollinated varieties and hybrids can be appropriately relaxed. 7.2.2 Cross-pollinated varieties
Cross-pollinated varieties include inbred lines that are more likely to show differences within the variety than asexual varieties, and are more difficult to distinguish between heteromorphic plants. The degree of variation of the variety shall be determined by comparison with known closely related varieties or types, and the consistency of the variety shall not be significantly lower than that of the closely related varieties. 7.2.2.1. The single trait
is required for the purpose of verifying the acceptable degree of variation of the variety. The acceptable degree of variation of the variety cannot be significantly reversed by the variation of its closely related varieties. 7.2.2.2.| The degree of variation of the variety shall be significantly higher than that of the closely related varieties. Specific international plant variety control measures include the "4 years of comprehensive analysis of the recognition of the heart", 7.2.3 The determination of the consistency of the hybrids is based on the cross between the hot and cold varieties, and whether the hybrids are made by the cross between the white emperor cattle or the cold-blooded varieties. The hybrids can be tested under certain conditions to determine the stability and stability of the hybrids. 7.2.3.1, from the self-crossing The method for judging the single-cross
of the pollinated varieties is to ensure that the percentage of self-pollination cannot be determined in the planting experiment: the DLS test guide for new plant varieties is related to the maximum allowable value. 7.2.3.2 For other types of single-cross
varieties with at least one parent being cross-pollinated, as long as there are other methods for judging the consistency of flowering and pollination varieties or comprehensive varieties, the method can be the same as that for flowering and pollination varieties.7.2.3.3 Other hybrids
For non-monopoly hybrids: three-cross and modified crosses, some characteristics will produce segregation, and the commercial selection should be in line with the current acceptable microbial breeding of the variety. The method for determining the sex of non-monopoly hybrids is as follows: If the genetic characteristics of the individual characteristics that require segregation are known, the method for determining the sex of the non-monopoly hybrids is as follows: 1) If the characteristics of the segregation characteristics are not difficult to detect, the other characteristics of the cross-pollination variety shall be determined according to the determination method of other characteristics of the cross-pollination variety. That is, the variety should be compared with its similar varieties to determine the maximum allowable value of self-pollination bacteria, see the cross-breeding method. 7.3 Unrelated and completely different plants
The test report may contain plants that are completely different or unrelated to the test variety. People cannot classify them as heteromorphic plants, nor can they classify them as the variety: if the presence of these plants does not affect the required test characteristics or test progress: they can be ignored. On the contrary, the state should ignore the road,
8 Qualitative judgment method
8.1 Related or main characters
GB/T 19557.1-2004
should include all the characters used in the DUS and the characteristics mentioned in the product at the time of authorization, regardless of whether they appear in the test. 8.2 Stability judgment
If there are varieties that meet the requirements of the release of the quality, it can be considered that the variety has the process of qualitative. If the variety does not have stability and the breeder can not provide effective breeding materials with the characteristics of the variety, then the variety must be planted for a long time or the seeds of the new variety must be planted for a long time. Whether the characteristics of the previous cross-comparison materials are consistent with the specifications: whether it is stable or not. Only the document on qualitative testing (GP/11) of the International Plant Variety Protection Association can be used.
In addition to testing the stability of the test itself, the stability of the test is also determined through the effectiveness of the test case. 9 Composition of the test guide
9.1 The scope of the test of new varieties is determined by the direct species genus and as the basic classification. If the differences between the species within the species are small, the genus is used as the basic unit. In addition, if the differences are large, the winter species should be used as the basic unit. In special cases, if the differences between subspecies within a species are very significant, the subspecies can be considered as the basic unit. 9.2 Contents of the test guide
a. General technical requirements of the test
h. Character table:
) Character explanation:
small) Technical volume,
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